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Citations for Thermo Fisher :
6751 - 6800 of 10000+ citations for rno mir 134 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: PCR reactions were performed on a Real-time PCR System (7500 Real-time PCR System, Applied Biosystems, Thermo Fisher Scientific, Foster City, CA, USA) following the programs ...
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bioRxiv - Microbiology 2022Quote: ... and gene-specific primers were used to perform quantitative reverse transcription-PCR (qRT-PCR) on 5 ng/μL cDNA in a Real-Time PCR System (Applied Biosystems ViiA 7, USA). Table S2 lists the primers used in qRT-PCR ...
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bioRxiv - Neuroscience 2023Quote: ... Quantitative PCR was performed using the Applied Biosystems StepOnePlus Real-Time PCR System with SYBR Green PCR master mix (Life Technologies, cat. no. 4364344) Primer sets used included ...
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bioRxiv - Microbiology 2023Quote: ... RNA samples were analyzed by qRT-PCR with Thunderbird Probe One-step qRT-PCR Kit (Toyobo) and QuantStudio 7 Flex Real-time PCR system (Applied Biosystems; Thermo Fisher Scientific). Target RNA levels were normalized to hamster Actb and calculated by the relative standard curve method ...
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bioRxiv - Cell Biology 2023Quote: The novel entry modules created for this study were cloned by insertion of PCR-amplified sequences in pJet1.3 or pMiniT 2.0 following manufacturer’s instructions (CloneJET PCR Cloning Kit, Thermo Scientific; NEB® PCR Cloning Kit, NEB). BsaI recognition sites followed by module-specific overhangs were added to each primer used to amplify entry sequences ...
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bioRxiv - Cancer Biology 2023Quote: ... and 3 of BCL2L1 were amplified by PCR and cloned into pCR-Blunt-TOPO vector using Zero Blunt TOPO PCR Cloning Kit (Thermo Fisher Scientific Cat# 450245) The primers used for PCR are exon 1&2 F1 ...
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bioRxiv - Microbiology 2020Quote: ... all PCR products were ligated into the pCR®-Blunt II-TOPO® Vector (Invitrogen) according to the manufacturer’s recommendation.
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bioRxiv - Plant Biology 2019Quote: ... The PCR amplified cDNA construct was purified using the GeneJET PCR Purification Kit (ThermoFisher Scientific) according to the manufacturer’s instructions and then separated on 6% polyacrylamide gel ...
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bioRxiv - Neuroscience 2021Quote: ... Quantitative PCR was conducted on a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems) using 12 ng cDNA per reaction ...
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bioRxiv - Cancer Biology 2021Quote: ... The PCR amplifications were performed using the SYBR-Green PCR Master Mix (Applied Biosystems, USA). Evaluation of relative RNA levels was carried out with 2−ΔΔCt method ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR products were cloned using the Zero Blunt PCR Cloning Kit (Thermo Fisher Scientific, K270040) and 20 clones were sequenced per cell line to verify successful genome editing.
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bioRxiv - Developmental Biology 2021Quote: ... Gene expression was quantified by real-time PCR (StepOnePlus Real-Time PCR System, Applied Biosystems) using 1 μL of cDNA ...
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bioRxiv - Cell Biology 2020Quote: ... standard qRT-PCR was carried out with a StepOnePlus Real-Time PCR system (Applied Biosystems) using TaqMan Fast Universal PCR Master Mix (Applied Biosystems) ...
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bioRxiv - Cell Biology 2019Quote: ... Real-time PCR was carried out on the 7500 Real-Time PCR System (Thermo Fisher), using a pre-PCR step of 20 seconds at 95°C ...
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bioRxiv - Cell Biology 2020Quote: ... qRT-PCR was performed on Step One Plus Real-Time PCR System (Applied Biosystems, USA) with SYBR Premix Ex Taq II Kit (Clontech Laboratories ...
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bioRxiv - Cell Biology 2020Quote: ... The PCR reaction was performed on a ViiA 7 Real-Time PCR System (Applied Biosystems). Samples were run in triplicate and analyzed using comparative CT (2-ΔΔCT ...
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bioRxiv - Developmental Biology 2021Quote: ... Real-time PCR was performed using Power SYBR Green PCR Master Mix (Applied Biosystems 4367659). Thermal conditions were 2 min at 50 °C ...
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bioRxiv - Immunology 2021Quote: ... cDNA was used for subsequent PCR amplification using the viia7 Real-time PCR system (Thermofisher) and the Fast SYBR Green Master Mix (Thermofisher) ...
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bioRxiv - Microbiology 2021Quote: ... PCR products were analyzed on an ABI 7500 Fast real-time PCR system (Applied Biosystems).
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bioRxiv - Molecular Biology 2021Quote: ... qRT-PCRs were performed using the SuperScript III Platinum One-Step qRT-PCR kit (ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Quantitative PCR (qPCR) was performed in a StepOnePlus Real-Time PCR system (Thermo Fisher Scientific). Reaction conditions were optimised using a gradient PCR ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Purification of the PCR products used ExoSAP-IT™ PCR Product Cleanup Reagent (ThermoFisher Scientific) and products were then sent to GATC Biotech for sequencing ...
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bioRxiv - Developmental Biology 2020Quote: ... Resulting clones were screened by PCR (DreamTaq Green PCR Master Mix, thermo Fisher Scientific; K1081), restriction digest ...
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bioRxiv - Neuroscience 2022Quote: ... qRT-PCR was carried out with Power SYBR Green PCR Master Mix (Applied Biosystems, #4367659). Two pairs of primers were used to amplify two different exonic regions of the PCDH19 transcript (5’ end ...
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bioRxiv - Plant Biology 2022Quote: ... qRT-PCR was performed using a StepOne Real-time PCR Instrument (Applied Biosystems, CA, USA) with KAPA SYBR FAST PCR Master Mix (Kapa Biosystems ...
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bioRxiv - Microbiology 2022Quote: ... PCR products and restriction digest products were purified using GeneJET PCR purification kit (Thermo Fisher) per manufacturer protocols ...
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bioRxiv - Molecular Biology 2021Quote: ... qRT-PCR was carried out on QuantStudio 3 Real-Time PCR System (Thermo Fisher Scientific) with iTaq SYBR Green Supermix (Bio-Rad Laboratories).
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bioRxiv - Molecular Biology 2020Quote: ... Real-time PCR reactions were cycled in StepOne™ Real-Time PCR System (Applied Biosystems) using the following primers in Supplemental Materials ...
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bioRxiv - Molecular Biology 2020Quote: ... and the PCR product was inserted into pCR-Blunt II-TOPO vector (Thermo Fisher Scientific). Furthermore ...
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bioRxiv - Molecular Biology 2021Quote: ... qRT-PCR was performed on a QuantStudio Real-Time PCR Light Cycler (Thermo Fisher Scientific) in technical triplicates ...
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bioRxiv - Molecular Biology 2019Quote: ... PCR amplicons were first cloned in pJet1.2/blunt (CloneJet PCR Cloning Kit; Thermo Fisher Scientific) and then subcloned into conjugative plasmid ...
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bioRxiv - Microbiology 2019Quote: ... PCR products and restriction reactions were purified using the PureLink Quick PCR Purification Kit (Invitrogen). When needed ...
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bioRxiv - Genomics 2020Quote: ... The PCR was performed in a QuantStudio 5 Real-Time PCR System (Thermo Fisher Scientific) using the following parameters ...
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bioRxiv - Developmental Biology 2020Quote: ... qRT-PCR was performed with Power Sybr Green PCR Master Mix (Thermo Fisher Scientific, 4367659) on the Applied Biosystems ViiA™ 7 Real-Time PCR System ...
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bioRxiv - Molecular Biology 2020Quote: ... Genotyping PCR was performed using Platinum green hot start PCR master mix (Invitrogen, Carlsbad, CA). Lysis buffer components ...
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bioRxiv - Cell Biology 2019Quote: ... cloned into the pCR-Blunt TOPO vector (Zero Blunt TOPO PCR cloning kit, ThermoFisher Scientific), and transformed into TOP10-competent E ...
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bioRxiv - Genomics 2019Quote: ... qRT-PCR reactions were performed in a 7500 Fast Real-Time PCR system (Applied Biosystems) with a GoTaq® qPCR master mix kit (Promega) ...
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bioRxiv - Physiology 2020Quote: ... qRT-PCR was performed using a ViiA Real-Time PCR System (Applied Biosystems, California, USA) using SYBR green master mix (Promega ...
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bioRxiv - Genetics 2019Quote: ... Real-time PCR was performed on ABI-7500 real-time PCR machine (Applied Biosystems, USA) and β-actin was used to normalize the expression values(67).The primer sequences used in qRT-PCR are listed in tables 1 ...
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bioRxiv - Immunology 2019Quote: ... Real-time PCR was performed with Taqman Fast Universal PCR Mastermix (4352042, Thermo Fisher Scientific) and Taqman Gene Expression Assays (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... The PCRs were performed in a ProFlex PCR System (Applied Biosystems via ThermoFisher Scientific, Germany) with the following cycling conditions ...
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bioRxiv - Microbiology 2020Quote: ... The PCRs were performed in a ProFlex PCR System (Applied Biosystems via ThermoFisher Scientific, Germany) with the following cycling conditions ...
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bioRxiv - Cancer Biology 2019Quote: ... qRT-PCR assay was performed by using the 7500 Real Time PCR machine (Applied Biosystems) with FastStart Universal SYBR Green Master (ROX ...
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bioRxiv - Immunology 2020Quote: ... This PCR fragment was cloned into the pCR-Blunt TOPO kit (ThermoFisher Scientific, cat. K280002) and DNA from 7-10 clones from before and after FLPo-mediated recombination was prepared by miniprep (Qiagen ...
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bioRxiv - Cell Biology 2020Quote: ... PCR products were then purified using the GeneJET PCR Purification Kit (Thermo Fisher, Waltham, MA) and used as templates for PCR amplification using the WIP1-F1 and WIP1-R2 primer set ...
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bioRxiv - Microbiology 2019Quote: ... Real-time quantitative PCR analysis was performed (Applied Biosystems ViiA 7 Real-Time PCR System).
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bioRxiv - Physiology 2019Quote: ... PCR products were column-purified with PureLink Quick PCR Purification Kit (Life Technologies, Burlington, ON) and amplified in a subsequent PCR reaction to generate cDNA products with incorporated T7 promoter sequence (see Table S1 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... PCR amplifications were performed with Phusion High Fidelity PCR Master Mix (Invitrogen, Carlsbad CA, USA). PCR conditions included an initial denaturation step of 98 °C for 30 s ...
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bioRxiv - Cell Biology 2019Quote: ... The PCR product was purified using a PureLink® PCR Purification Kit (Invitrogen, K3100-01), digested by NheI-HF and XhoI restriction enzymes (New England Biolabs ...
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bioRxiv - Plant Biology 2019Quote: ... PCR reactions were then cleaned using ExoSAP-IT PCR Product Cleanup Reagent (ThermoFisher, Waltham, MA). Sequencing reactions were performed with the forward and reverse primers for each of the three regions ...