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Citations for Thermo Fisher :
6951 - 7000 of 10000+ citations for 2 3 5 6 Tetrahydroxy 4 phosphonooxycyclohexyl dihydrogen phosphate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... samples were first boiled with 6x Laemmli SDS buffer ar 95°C for 5 minutes and proteins were separated on Bolt 4-12% Bis-Tris gels (Thermo Fisher, NWO4122BOX and NWO4127BOX). Gels were washed in water ...
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bioRxiv - Neuroscience 2023Quote: ... or Tris-Glycine-SDS sample buffer (Novex) at 95°C for 5 min and separated using 4–20% Tris-glycine (ThermoFisher, #EC6025 or Invitrogen # XP04200). Proteins were transferred to nitrocellulose membranes (BioRad ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were first boiled with 6x Laemmli SDS buffer at 95°C for 5 minutes and then proteins were separated on Bolt 4-12% Bis-Tris gels (Thermo Fisher, NWO4122BOX and NWO4127BOX), transferred to Nitrocellulose membranes using the Mini Trans-Blot Cell (BioRad ...
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bioRxiv - Neuroscience 2023Quote: For FVB and P301L brain microscopy, animals were sequentially transcardially perfused with PBS at Janssen Laboratory (Beerse, Belgium) 4% paraformaldehyde (PFA) (Affymetrix USB. J19943; 5 min) at 4 ml/min ...
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Bacterial Polyphosphates Induce CXCL4 and Synergize with Complement Anaphylatoxin C5a in Lung InjurybioRxiv - Immunology 2022Quote: ... the cells were washed in ice-cold sterile PBS (650g, 5 min, 4°C) and stained for 30 min with fixable viability dye eFluor 780 (Thermo Fisher Scientific, 1:1000) using heat-killed (1 min ...
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bioRxiv - Genomics 2022Quote: ... bone marrow was extracted and rinsed in a small glass Petri dish with 5 ml chilled Roswell Park Memorial Institute (RMPI) 1640 (Gibco, chilled at 4°C) culture medium and then transferred to a centrifuge tube containing 10 ml RPMI ...
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Optimisation of immunocytochemistry methodology for the detection of endogenous eIF2B localised focibioRxiv - Cell Biology 2022Quote: ... Cells were then washed with PBST 4 times for 5 min and mounted using ProLong™ Gold Antifade Mountant with DAPI 220 (Invitrogen, Thermo Fisher Scientific). Cells were viewed on a Zeiss LSM 800 confocal microscope.
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bioRxiv - Immunology 2023Quote: ... NIKWT and NIKKO BMDMs were treated with 20ng/mL IL-4 for 5 hours before cells were detached using PBS containing 1mM EDTA (Gibco, ThermoFisher Scientific; Waltham, MA, USA). Cells were then stained with a combination of Mitotracker Green FM ...
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bioRxiv - Microbiology 2024Quote: ... Each pooled seagrass sample was homogenized in a BashingBead Lysis Tube containing 2 mm ceramic beads and 800 μL RNA lysis bu?er (provided in the kit) for 5 minutes at maximum speed using a FisherbrandTM Bead Mill 4 Homogenizer (Fisher Scientific, Waltham, MA, USA). Tissue homogenates were centrifuged at maximum speed (21,130 g ...
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bioRxiv - Immunology 2024Quote: ... The cells were washed in serum-free cRPMI medium and treated with 5 nM Fluo-4 calcium indicator dye (Invitrogen, Thermo Fisher Scientific, Massachusetts, USA) with Pluronic (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... Protein samples were denatured at 100 °C for 5 minutes and briefly centrifuged before loading onto a 4-12% Tris-Glycine Novex Wedgewell gel (Fisher Scientific, Cat no. XP04125BOX). SDS-PAGE was performed using standard running buffer in Xcell Surelock Electrophoresis Cell (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... The pellet was collected by centrifugation at 300x g at 4°C and incubated in 5 mL of 1x RBC lysis buffer (Thermo Fisher Scientific, Massachusetts, USA) per spleen for 5 min at room temperature with occasional shaking to remove blood cells ...
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bioRxiv - Developmental Biology 2024Quote: ... aegypti embryos at specified developmental stages (0-1h, 4-5h, 5-6h, 23-24h, 47-48h, and 71-72h) using Trizol® reagent (Ambion by Life Technologies), following the manufacturer’s instructions ...
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Direct analysis of ribosome targeting illuminates thousand-fold regulation of translation initiationbioRxiv - Molecular Biology 2020Quote: ... and 3′ biotinylated using the Pierce RNA 3′end biotinylation kit (Thermo Scientific 20160).
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bioRxiv - Molecular Biology 2021Quote: ... counted and reseeded in 3 mL A medium (1:3 mix DMEM/F12 (Gibco) and Neurobasal (Gibco) ...
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bioRxiv - Cell Biology 2021Quote: ... 3 μl were sampled on a 3 well Diagnostika slides (X1XER303B) from Thermo scientific for observation on an Zeiss LSM710 confocal microscope equipped with a Plan-Apochromat 63×/1.4 Oil objective and 405 nm and 488 nm lasers ...
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bioRxiv - Neuroscience 2022Quote: ... 3 μg DNA and 3 μL Lipofectamine in 300 μl Optimem (Thermofisher Scientific, USA) were used per well containing 700 μl DMEM ...
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bioRxiv - Bioengineering 2023Quote: ... and EDC (1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... wells were treated with Caspase-3/7 (CellEvent™ Caspase-3/7 Green, Invitrogen) 1:1000 in treatment media ...
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bioRxiv - Microbiology 2023Quote: ... 3′RNA-seq libraries were analyzed on a Qubit 3 Fluorometer (Thermo Fisher Scientific) and an Agilent 4200 TapeStation System prior to paired- end sequencing using the HiSeq 2500 system (Illumina).
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bioRxiv - Neuroscience 2024Quote: ... 3 mg of solid red DiI (1,1’-dioctadecyl-3,3,3’,3’-tetramethylindocarbocyanine perchlorate, Molecular Probes) dissolved in methylene chloride were mixed with 50 mg of tungsten beads (1.3 microns in diameter ...
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bioRxiv - Bioengineering 2022Quote: ... 4 μl of 4 mM resazurin sodium salt (Acros Organics, Belgium), 0.002 U purified Castellaniella defragrans geraniol dehydrogenase ...
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bioRxiv - Cell Biology 2022Quote: ... Itgb1 (forward 5’-ATGCCAAATCTTGCGGAG AAT, reverse 5’-TTTGCTGCGATTGGTGACATT), and GAPDH (forward 5’– GGTCATCCATGACAACTT, reverse 5’–GGGGCCATCCACAGTCTT) are designed and synthesized by Invitrogen (Shanghai, China).
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bioRxiv - Evolutionary Biology 2020Quote: ... Cellular debris was removed by centrifugation at 5,488x g for 40 min at 4°C (Heraeus™ Megafuge™ 40R) and incubated for 2 h with 500 μl HisPurTM Cobalt Resin (89964, ThermoFisher Scientific) at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Zebrafish larvae were incubated in 1x E3-medium buffered with 4mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) (Life Technologies, Carlsbad, CA, USA). Seahorse XF Cell Mito Stress kit (Agilent Technologies ...
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bioRxiv - Systems Biology 2020Quote: ... and 4 μL of this dilution were used to transform One ShotTM ccd B Survival 2 T1R competent cells (Thermo Fisher Scientific, A10460). Transformants were selected with 33 μg/mL chloramphenicol (Merck ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated with goat anti-mouse IgG1 Alexa 488 secondary antibody (1:500 for 2 h at 4°C; Invitrogen by ThermoFisher Scientific) in solution with PBS ...
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bioRxiv - Immunology 2020Quote: ... Lysate equivalent to 2×106 cells per well was run on a NuPAGE 4 to 12% Bis-Tris gel (Thermo Fisher Scientific, USA). Western blots were performed on samples and membranes were probed with anti-PTPN22 (clone D6D1H ...
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bioRxiv - Biochemistry 2021Quote: ... The cells were then fixed by resuspending the cell pellet in 100 uL 2% paraformaldehyde (PFA) - 4% solution diluted in Pbs (Invitrogen, Thermo Fisher, UK) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... 1000 μg of total protein was incubated overnight at 4 °C with 2 μg α-GFP (Life Technologies/ThermoFisher Scientific, Carlsbad, CA, USA) and 20 μL of a 50% slurry of sepharose Protein A (Rockland Immunochemicals ...
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bioRxiv - Microbiology 2021Quote: ... The denatured protein samples were separated by Novex™ WedgeWell™ 4-20% SDS-PAGE Tris-Glycine gel and transferred to PVDF membrane (iBlot™ 2 Transfer Stacks, Invitrogen) using iBlot 2 Dry Blotting System (Cat ...
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bioRxiv - Molecular Biology 2020Quote: ... The elute was run 2 cm into a 1.5 mm NuPAGE™ Novex™ 4-12% Bis-Tris protein gel (Thermo Fisher Scientific), stained with Coomassie blue and distained with distaining solution (10% acetic acid and 50% methanol) ...
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bioRxiv - Microbiology 2023Quote: ... The number of copies was calculated from the standard curve obtained from running pCR 4-TOPO or pCR-XL-2-TOPO plasmid (ThermoFisher, Waltham, Massachusetts, USA) containing an insert spanning ITS1-5.8S rRNA-ITS2-D1/D2 region of 28S rRNA from a pure culture strain ...
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bioRxiv - Cancer Biology 2023Quote: ... and primary antibodies were incubated overnight at 4°C before a 2-h incubation with secondary antibodies (1:500, Invitrogen, #A21428 and #A21235) diluted in blocking buffer ...
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bioRxiv - Microbiology 2023Quote: ... An equivalent of 2×107 parasites whole protein lysate was loaded on a NuPAGE 4-12% Bis-Tris protein gel (Invitrogen, cat log # NP0321BOX) followed by transfer onto a PVDF membrane (Thermo Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... After a clarifying spin for 15 min at 14,000 ×g and 4°C 2 μl (MICOS complex) or 7 μl (all other mitochondrial protein complexes) G-250 Sample Additive (Invitrogen, Waltham, Massachusetts, USA) containing 40% glycerol were added ...
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bioRxiv - Cell Biology 2022Quote: ... washed with PBS and stained overnight at 4 °C with 20 μg/mL of LAMP-2 primary antibody (Thermo Fisher Scientific, MA, USA) in 1 % BSA (in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were incubated with goat anti-rabbit IgG Alexa 488 secondary antibody (1:500 for 2 hours at 4°C; Invitrogen by ThermoFisher Scientific) in solution with PBS ...
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bioRxiv - Microbiology 2023Quote: ... [72] at 4 °C overnight followed by staining with 2 µg/ml of secondary AlexaFluor647-conjugated Donkey anti-human IgG antibody (ThermoFisher Scientific, OR, USA) at room temperature for 45 min ...
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bioRxiv - Neuroscience 2024Quote: ... They were then mechanically chopped at the start of the glial enrichment phase and cultured for 2-4 weeks in base medium supplemented with EGF (Gibco, 20 ng/ml) and leukaemia inhibiting factor (LIF ...
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bioRxiv - Genomics 2024Quote: ... After 2–4 weeks the clones were screened for homologous deletion using the kit Phire Tissue Direct PCR Master Mix (Thermo Fisher Scientific, F170L). Clones with homologous insertion were those showing a mutant band of the expected size and no wild-type band ...
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bioRxiv - Genomics 2024Quote: ... After 2–4 weeks the clones were screened for homologous deletion using the kit Phire Tissue Direct PCR Master Mix (Thermo Fisher Scientific, F170L). Clones with homologous deletion were those showing a mutant band of the expected size and no wild-type band ...
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bioRxiv - Physiology 2024Quote: ... were aspirated using a 21-gauge needle attached to a 5 mL disposable syringe in the presence of 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES)-buffered TCM-199 medium (Gibco, Life Technologies, Milan, Italy) and 0.005% (w:v ...
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bioRxiv - Developmental Biology 2024Quote: ... The IVM medium consisted of 25 mM 4-(2-hydroxyethyl) piperazine-1-ethanesulfonic acid-buffered tissue culture medium 199 (M199; Gibco, Paisley, Scotland, UK) supplemented with 5% calf serum (CS ...
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bioRxiv - Immunology 2021Quote: ... 3 μM Hoechst (Life Technologies) was added to each well to stain nuclei for cell counting ...
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bioRxiv - Immunology 2021Quote: ... 3 μM Hoechst (Life Technologies) was added to each well to stain nuclei for cell counting ...
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bioRxiv - Genomics 2021Quote: ... 3 mL (Thermo Fisher Scientific) overnight at 4°C ...
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bioRxiv - Genomics 2020Quote: ... 3 washes 5x SSCT (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... 3 washes 5x SSCT (ThermoFisher Scientific 15557044 ...
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bioRxiv - Immunology 2021Quote: ... supplemented with 3% FBS (Gibco) and 100 U/mL penicillin-streptomycin (Gibco) ...