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Citations for Thermo Fisher :
6901 - 6950 of 10000+ citations for 2 3 5 6 Tetrahydroxy 4 phosphonooxycyclohexyl dihydrogen phosphate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... on a QuantStudio™ 6 Flex (Applied Biosystems), where β-actin was examined as an internal control for normalization ...
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bioRxiv - Synthetic Biology 2024Quote: ... 6-well plates in E8 medium (Gibco: A1517001). Kidney organoids were derived using a previously published differentiation protocol 21,37 ...
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bioRxiv - Cell Biology 2024Quote: ... 6-well plate in E8 (Gibco, MA, USA) medium supplemented with Y-27632 ROCK Inhibitor (10uM ...
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bioRxiv - Neuroscience 2021Quote: ... PCR amplification was used to add NotI/XbaI restriction sites to the 5’ and 3’ ends followed by digestion and ligation into a modified version of the pUASp vector (Invitrogen; kindly provided by Tom Millard) which confers ampicillin resistance and tags the construct N-terminally with eGFP (referred to as pUASp-eGFP) ...
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bioRxiv - Bioengineering 2023Quote: Single colonies were inoculated in 3 mL LB broth (10 g/L peptone, 5g/L yeast extract, and 5 g/L NaCl) (Thermo Fisher Scientific, Waltham, MA, USA) containing the respective antibiotics at 37°C at 220 rpm overnight ...
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bioRxiv - Immunology 2023Quote: ... D1-3 and D4-5 proteins were fluorescently labelled with Alexa Fluor™ 594 or 488 Microscale Protein Labeling Kits (Invitrogen™, A30008 or A30006) as described [15] ...
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bioRxiv - Cell Biology 2021Quote: ... 4% KSR (ThermoFisher), 1% ITS-X supplement (ThermoFisher) ...
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bioRxiv - Cell Biology 2021Quote: ... 4% KSR (ThermoFisher), 1% ITS-X supplement (ThermoFisher) ...
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bioRxiv - Genetics 2021Quote: ... SSEA-4 (ThermoFisher), Sox2 (Epitomics ...
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bioRxiv - Physiology 2021Quote: ... fluo-4 (Invitrogen). Ca2+ and Lifeact images were acquired at 1 image/10 seconds.
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bioRxiv - Bioengineering 2022Quote: ... 4% Glutamax (ThermoFisher), 1% Sodium Pyruvate (ThermoFisher ...
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bioRxiv - Bioengineering 2022Quote: ... 4% Glutamax (Gibco), 1% Sodium Pyruvate (Gibco ...
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bioRxiv - Microbiology 2021Quote: ... 4 % FCS (Gibco), 200 units/mL penicillin and 0.2 mg/ mL streptomycin ...
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bioRxiv - Genomics 2021Quote: ... 4% Glutamax (Gibco), 1% Sodium Pyruvate (Gibco ...
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bioRxiv - Cancer Biology 2020Quote: ... 4% Glutamax (Gibco), 1% Sodium Pyruvate (Gibco ...
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bioRxiv - Molecular Biology 2023Quote: ... 4% B27-(ThermoFisher), 100 µM Palmitate (Sigma) ...
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bioRxiv - Microbiology 2024Quote: ... claudin-4 (Invitrogen), occludin (Cell Signaling Technologies) ...
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bioRxiv - Microbiology 2024Quote: ... claudin-4 (Invitrogen), occludin (Cell Signaling Technologies) ...
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bioRxiv - Cell Biology 2020Quote: ... Grids were blotted for 2-3 seconds before plunge freezing in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific, Waltham, MA, USA) at 4 °C and 100% humidity.
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bioRxiv - Neuroscience 2021Quote: ... Hippocampal neurons were seeded onto polyethylenimine-coated 3-cm dishes (2 × 105 cells/dish) and cultured in neurobasal medium (Gibco; Thermo Fisher, Waltham, MA, USA) supplemented with B-27 and 2 mM Glutamax (Gibco ...
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bioRxiv - Plant Biology 2021Quote: ... proteins were digested with both trypsin and chymotrypsin and the resultant peptides were loaded onto a reverse phase C18 trap column (Acclaim PepMap 100, 75 μm x 2 cm, nano viper, C18, 3 μm, 100 Å, ThermoFisher, Waltham, MA, U.S.A) using an Ultimate 3000 and washed with 50 μL of solvent A at 10 μl/min ...
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bioRxiv - Neuroscience 2020Quote: ... CO slices were perfused at a rate of 2-3 mL/min in oxygenated MitoTracker staining solution containing: ACSF and MitoTracker™ Red CMXRos (Invitrogen™, M7512, 100nM) for 30 minutes at 29–32°C in the dark ...
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bioRxiv - Cell Biology 2021Quote: ... We isolated neonatal cardiomyocyte (postnataTl 2 – 3 days) from Gja1M213L/M213L and Wildtype (WT) mice using PierceTM Primary Cardiomyocyte Isolation Kit (Thermo Fisher Scientific, Walthman, MA) following manufacturer protocol ...
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bioRxiv - Neuroscience 2023Quote: ... and IVH-PHH (Supplementary Figure 2) P7 rats underwent intraventricular injection of 20 µl of 3 kDa Red Dextran Tetramethyl rhodamine (RD/TMR) (D3307, Thermo Fisher Scientific, Waltham, MA), 1.9-nm (1102 ...
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bioRxiv - Physiology 2022Quote: ... Brains were quickly transferred to 10 μL of the same saline in a 200 μL microcentrifuge tube kept at 2-3°C in a Boekel Scientific TropiCooler Benchtop Incubator (model 260014, Fisher Scientific, Ottawa, ON, Canada), and once 30 brains were collected (2-2.5 min per brain ...
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bioRxiv - Microbiology 2023Quote: ... determined by preparing a slurry with 1 part soil to 2 parts deionized water and measuring (n=3) with an Orion Star A111 pH Meter (Thermo Scientific, Waltham, MA, USA), was 5.87 ± 0.42 in November 2020 and 6.28 ± 0.07 in April 2021.
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bioRxiv - Immunology 2024Quote: Vero-CCL81 [for MuV and VSV_RUBV] or Vero-hSLAM [for MeV-IC323] target cells which were seeded at a density of 2 × 104 cells per well in flat-bottom 96-well plates (Fisher Scientific, #08-772-3). The cells were incubated at 37 °C/5% CO2 overnight (∼20 hours) ...
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bioRxiv - Physiology 2023Quote: ... HBMECs were washed by 2% BSA (3×, 5min) followed by secondary antibody incubation with either Alexa Fluor 488 goat anti-mouse (ThermoFisher Scientific; Catalogue number: A11001) or Alexa Fluor 555 goat anti-rabbit (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... organoids were washed 3 times in DPBS and incubated for 2 hours with Alexa Fluor 647-conjugated anti-mouse secondary antibody (Thermo Fisher Scientific, 1:1000) in blocking buffer at RT ...
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bioRxiv - Biochemistry 2023Quote: ... and ∼5-10 µL of peptide was loaded onto a reverse-phase trap column (Acclaim PepMap 100, 75 µm × 2 cm, NanoViper, C18, 3 µm, 100 Å, ThermoFisher, Waltham, MA, USA) using an Ultimate 3000 autosampler and washed with 40 µL of buffer A at a flow rate of 20 µL min−1 ...
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bioRxiv - Systems Biology 2024Quote: ... 2-3 butanediol, and erythritol), and organic acids (acetate, succinate, tartrate, citrate, and malate) were determined using an HPLC (Thermo Fisher Scientific, Waltham, MA) equipped with a refraction index and UV/VIS (210nm ...
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bioRxiv - Pathology 2024Quote: ... and predesigned TaqMan-based qPCR assays for target and housekeeping genes (Table 2) and run on the 7500 Fast Real-Time PCR System and QuantStudio 3 thermocyclers (Applied Biosystems; Waltham, CA, USA). Relative expression values were normalized by the expression of housekeeping genes using the 2-ΔΔCt method ...
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bioRxiv - Plant Biology 2024Quote: ... Peptides were pre-concentrated on an Acclaim PepMap 100 pre-column (75 µM x 2 cm, C18, 3 µM, 100 Å; Thermo Fisher Scientific, USA) using the loading pump and buffer A (water ...
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bioRxiv - Bioengineering 2024Quote: ... consisting of a trap column (C18, 75 µm ID x 2 cm, particle size 3 µm pore size 100 Å; Thermo Fisher Scientific, 164946) and an analytical column (75 µm ID x 50 cm ...
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bioRxiv - Immunology 2022Quote: ... 0.5% 2-mercaptoethanol (2-ME) (GIBCO) and 1% HEPES (GIBCO) ...
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bioRxiv - Systems Biology 2021Quote: ... The cells were maintained in fibroblast media for 6 days then passaged onto Matrigel-coated dishes for further reprogramming in Essential 6 medium plus human bFGF (ThermoFisher Scientific). The iPSC colonies were manually picked onto Matrigel-coated plates to generate stable iPSC lines ...
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bioRxiv - Systems Biology 2021Quote: ... The cells were maintained in fibroblast media for 6 days then passaged onto Matrigel-coated dishes for further reprogramming in Essential 6 medium plus human bFGF (ThermoFisher Scientific). The iPSC colonies were manually picked onto Matrigel-coated plates to generate stable iPSC lines ...
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bioRxiv - Developmental Biology 2022Quote: ... embedded in paraffin using the Tissue-Tek VIP 6 (Sakura) tissue processor and of 6 µm sections obtained with a microtome (HM355S, Thermo Scientific). Z-shaped probes for Sema6d (565871) ...
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bioRxiv - Systems Biology 2020Quote: 1×10^6 cells were seeded in wells of 6-well plates (FALCON # 353046) in MEM without phenol red (Gibco # 51200038) supplemented with 0.5% Fetal Calf Serum (VWR #89510-184) ...
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bioRxiv - Systems Biology 2020Quote: 1×10^6 cells were seeded in wells of 6-well plates (FALCON # 353046) in MEM without phenol red (Gibco # 51200038) supplemented with 0.5% Fetal Calf Serum (VWR #89510-184) ...
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bioRxiv - Systems Biology 2020Quote: 1×10^6 cells were seeded in wells of 6-well plates (FALCON # 353046) in MEM without phenol red (Gibco # 51200038) supplemented with 0.5% Fetal Calf Serum (VWR #89510-184) ...
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bioRxiv - Microbiology 2023Quote: ... cells were plated at 650,000 cells per well of a 6 well plate and infected with 6 dilutions from a 10-fold dilution series in 1% FBS (Gibco, MA)/1X non-essential amino acids (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... The IL-6 levels in plasma were analyzed using a Mouse IL-6 Uncoated ELISA kit (#88-7064, Invitrogen, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: Cells were seeded at 30 000 cells/cm2 and were treated 6 h later with 6 µM of the calcium-sensitive Rhod2AM probe (ThermoFisher Scientific) for 1 h before refreshing the cell culture medium ...
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bioRxiv - Biochemistry 2024Quote: ... Transfection of 1 µg per well of plasmids encoding SARS-CoV-2 tag-free nucleocapsid protein was performed using 6 µL per well of Lipofectamine 2000 on HEK-293T cells with 70-80% confluency on 6-well plates (Invitrogen, 11668027). Compounds were added to the cells with fresh media after 6 hours of incubation with plasmids for 24-hour treatment before cell lysis and immunoblotting ...
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bioRxiv - Molecular Biology 2020Quote: ... After RT we digested all residual oligos using adding 5 µl mastermix of 4 µl nuclease free H2O (DNase/RNase-Free Distilled H2O, Thermo Fisher Scientific #10977-049), 0.5 µl of Exonuclease I (NEB #M0568 ...
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bioRxiv - Developmental Biology 2021Quote: Preparation of lipid/cholesterol depleted fetal bovine serum (FBS): 500mL FBS were stirred overnight at 4°C with 10g Cab-osil M-5 (ACROS Organics 7631-86-9). The mix was then centrifuged for 10min at 3000rpm and the supernatant filtrated under sterile conditions ...
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bioRxiv - Physiology 2021Quote: ... Samples were incubated on ice for 5 min before cellular debris was pelleted by centrifugation at 10,000 rpm for 5 min at 4°C and supernatant was removed (Thermo Scientific, Heraeus Pico 21 centrifuge). Centrifugation was repeated twice more to remove fat from the samples ...
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bioRxiv - Molecular Biology 2021Quote: ... A sample from each pull-down (5% of the total eluate) was separated by 4-20% SDS-PAGE and proteins were visualized using SilverQuest Stain (Thermo Fisher Scientific Life Technologies). Fractions containing a protein with the predicted molecular weight of the bait (usually fractions 2 and 3 ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue was washed with PBSt 4×5 min and were mounted and coverslipped with Fluoromount Aqueous Mounting Medium with DAPI (Thermo Fisher, 00-4959-52). Slides were stored at 4°C.