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Citations for Thermo Fisher :
7101 - 7150 of 10000+ citations for 2 3 5 6 Tetrahydroxy 4 phosphonooxycyclohexyl dihydrogen phosphate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... the samples were loaded via an Acclaim PepMap 100 trap column (100 µm x 2 cm, nanoViper, C18, 5 µm, 100 Å; Thermo Scientific) onto an Acclaim PepMap RSLC analytical column (75 µm x 50 cm ...
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bioRxiv - Immunology 2020Quote: ... cells were resuspended at 5×106 cells/mL in 1 mM MgCl2/PBS and treated with 2 mM DSG cross-linker (Thermo Fisher Scientific) at room temperature for 30 min on a rotator ...
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bioRxiv - Neuroscience 2022Quote: ... except that they also received 2 injections of 10 mg/kg 5-ethynyl-2’-deoxyuridine (EdU) from the Click-iT EdU Alexa Fluor 647 imaging kit (Invitrogen, Carlsbad, CA), the day after DOX injection (4 DPN ...
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bioRxiv - Bioengineering 2022Quote: ... 2.2 mM LAP was added to half of the samples and 10 µM 5-ethynyl-2’-deoxyuridine (EdU) solution from a Click-iT Plus EdU Cell Proliferation Kit (cat. no. C10637; Invitrogen, Waltham, MA) was added to the other half according to manufacturer instructions ...
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bioRxiv - Cell Biology 2022Quote: ... HeLa cells (CCL-2; ATCC) were cultured at 37°C and 5% CO2 in DMEM/high-glucose/GlutaMAX medium (10564029; Thermo Fisher Scientific) supplemented with 10% fetal bovine serum Thermo Fisher ...
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bioRxiv - Immunology 2022Quote: ... 2 μL of combined 1μM forward and reverse primers (see Supplementary table 2) and 5 μL SYBR green master mix (#A25742; Applied biosystems, Waltham, USA) and run on the QuantStudio7Flex (Applied Biosystems ...
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bioRxiv - Molecular Biology 2022Quote: ... was mixed with 2 µl of recombinant yeast Poly(A) Polymerase (PAP) together with 5 µl of 5X PAP buffer (Thermo Fisher Scientific), 2.5 µl of 10 µM GTP/ITP/MgCl2 (Jena Bioscience) ...
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bioRxiv - Neuroscience 2022Quote: ... and the trap column used was Acclaim PepMap 100 (100 μm x 2 cm, nanoViper, C18, 5 μm, 100 Å, Thermo Scientific). Initial data analysis was performed using Proteome Discoverer (Thermo Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were loaded at 10 μL/min onto a trap column (100 μm × 2 cm, PepMap nanoViper C18 column, 5 μm, 100 Å, Thermo Scientific) equilibrated in 0.1% trifluoroacetic acid (TFA) ...
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bioRxiv - Microbiology 2024Quote: ... Peptides were trapped on an Acclaim Pepmap 100 C18 trap column (100 μm x 2 cm, particle size 5 μm, Thermo Fisher Scientific) and separated on an in-house packed analytical column (35 cm x 75 μm ...
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bioRxiv - Immunology 2024Quote: ... 2 μg of each sample were loaded at 15 μL/min onto a trap column (100 μm × 2 cm, PepMap nanoViper C18 column, 5 μm, 100 Å, Thermo Scientific) equilibrated in 0.1% TFA ...
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bioRxiv - Molecular Biology 2023Quote: ... Then 3 µl of sample at 0.07 mg/ml concentration were applied to the glow-discharged grids and blotted for 2 s with blotting force -5 using a FEI Vitrobot (Thermo Fisher Scientific) at a temperature 10 °C and 100% humidity ...
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bioRxiv - Neuroscience 2024Quote: ... Digests were loaded onto a trapping guard column (EASY-column, 2 cm long, ID 100 μm, packed with Reprosil C18, 5 μm particle size from Proxeon, Thermo Fisher Scientific) at a maximum pressure of 160 Bar ...
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bioRxiv - Cell Biology 2024Quote: Sor-treated myotubes on 18 mm laminin-coated coverslips were loaded with 5 µM Ca2+ indicator FURA-2 AM (#F1221, Thermo Scientific, US) along with 2.5 mM Probenecid (#P36400 ...
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bioRxiv - Cancer Biology 2024Quote: ... Reverse transcription (RT-PCR) was performed on 2-5 μg of RNA using the SuperScript III First-Strand Synthesis System (ThermoFisher Scientific, 18080051). Real time qPCR was conducted in a 384-well PCR microplate (Fisher scientific ...
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bioRxiv - Cell Biology 2024Quote: ... 12 alternating fractions were resuspended in 2% formic acid/5% acetonitrile and subsequently injected for analysis on an Orbitrap Eclipse Tribrid (Thermo Fisher Scientific). For the yeast cell PISA ...
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bioRxiv - Biochemistry 2023Quote: ... 1.5 μg aliquot of each sample were loaded at 15 μL/minute onto a trap column (100 μm × 2 cm, PepMap nanoViper C18 column, 5 μm, 100 Å, Thermo Scientific) equilibrated in 0.1% trifluoroacetic acid (TFA) ...
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bioRxiv - Cell Biology 2022Quote: ... Immunostained cells were then washed 2 times with DPBS for 5 minutes at room temperature and mounted on glass slides using Fluoromount G (Thermo Fisher Scientific) and a coverslip ...
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bioRxiv - Immunology 2023Quote: ... Duplicate wells of 200,000 CFSE-stained cells per sample were then incubated for 91 hours at 37°C (5% CO2) at a 1:2 bead-cell ratio with Human T-Activator CD3/CD28 DynaBeadsTM (Thermo Fisher, #11161D) and 1 U/mL of recombinant human IL-2 (BioLegend ...
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bioRxiv - Genetics 2023Quote: ... Ovaries were then washed 3 times for 5 min with PBTx followed by a blocking step in PBTx supplemented with 2% normal goat serum (NGS, Invitrogen, ThermoFisher Scientific) and 0.5% bovine serum albumin (BSA ...
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bioRxiv - Biophysics 2023Quote: ... Peptides were trapped on an Acclaim Pepmap 100 C18 trap column (100 μm x 2 cm, particle size 5 μm, Thermo Fisher Scientific) and separated on an in-house packed analytical column (75 μm x 35 cm ...
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bioRxiv - Cell Biology 2023Quote: ... Percent of cell death was determined by the staining with 5 µM Hoechst 33342 and 2 µM propidium iodide (PI) (Invitrogen, Carlsbas, CA). Images were taken and counted by Zeiss microscope equipped with automated computer-assisted software (Axiovision 4.6 ...
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bioRxiv - Cell Biology 2023Quote: ... Quantification of neuronal survival was determined by staining treated cultures with 5 µM Hoechst 33342 and 2 µM propidium iodide (PI) (Invitrogen, Carlsbad, CA) 1 ...
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bioRxiv - Cancer Biology 2023Quote: ... Peptides were trapped on an Acclaim Pepmap 100 C18 trap column (100 μm x 2 cm, particle size 5 μm, Thermo Fisher Scientific) and separated on an in-house packed analytical column (75 μm x 35 cm ...
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bioRxiv - Molecular Biology 2023Quote: ... Peptides from each fraction were resuspended in 50 µl 1% formic acid and aliquots of 4 μL were loaded at 10 μL/min onto a trap column (100 μm × 2 cm, PepMap nanoViper C18 column, 5 μm, 100 Å, Thermo Scientific) equilibrated in 0.1% TFA ...
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bioRxiv - Developmental Biology 2023Quote: COV434 cells were transfected with the plasmid cloned with the gRNA directed toward the 5’ region of exon 2 of the NR2F2 gene using Lipofectamine 3000 (Thermo Fisher Scientific). Blue fluorescence protein (BFP)-positive cells were observed and photographed using a fluorescence microscope (Zeiss ...
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bioRxiv - Biochemistry 2023Quote: ... and Caco-2 cells engineered to express ACE2 88 were cultured at 37°C in 5% CO2 in DMEM containing GlutaMAX (Gibco, Thermo Fisher) supplemented with 10% (v/v ...
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bioRxiv - Cancer Biology 2023Quote: ... Reactions were diluted 2–5 fold with H2O and qPCR was performed using PowerUp SYBR Green PCR Master mix (Thermo Fisher Scientific), 2 μL diluted cDNA preparation ...
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bioRxiv - Neuroscience 2023Quote: ... the fish were paralyzed before being mounted in agarose by bathing them for 2-5 min in a solution of 1 mg/mL α-bungarotoxin (Thermofisher Scientific) in E3 medium ...
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bioRxiv - Microbiology 2023Quote: ... Cells were transfected with 1 ng of coding plasmid diluted in 1.5 μg of pCEP4-△CMV carrier plasmid (77) per mL of culture at 2 ξ 106 cells/mL using Expifectamine (ThermoFisher Scientific). The medium was replaced 2 h post-transfection ...
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bioRxiv - Cancer Biology 2023Quote: Human/PDX/PBMC cells were resuspended in PBS+2% FBS at a final concentration of 10×106 cells/mL in 5 mL FACS tubes after filtering (Fisher Scientific #352235). Cells were blocked with mouse IgG (Sigma #I5381 ...
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bioRxiv - Cell Biology 2023Quote: ... intestinal fragments were incubated in epithelial cell solution (10 mM EDTA [Invitrogen, #15575020]-HBSS, 5 mM HEPES [Invitrogen, #15630080], 100 U/ml Pen-Strep [Invitrogen, #15140122], and 2% FBS [Life Technologies, #26140079]) for 30 min on ice with continuous gentle shaking ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Plates were measured at A470 and 25 °C every 5 min for 2 h using a Varioskan Flash multimode reader (Thermo Scientific, USA). For dose-dependent assays ...
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bioRxiv - Cell Biology 2023Quote: ... the cells (or fibers) were washed with PBS and counterstained with 4’6- diamidino-2-phenylindole (DAPI, 5 mg/ml, Molecular Probes, Invitrogen, Carlsbad, USA) in PBS to label nuclei.
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bioRxiv - Immunology 2023Quote: ... Samples were loaded at 10 μL/min onto a trap column (100 μm × 2 cm, PepMap nanoViper C18 column, 5 μm, 100 Å, Thermo Scientific) equilibrated in 0.1% trifluoroacetic acid (TFA) ...
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bioRxiv - Neuroscience 2023Quote: ... and the cultures were incubated at 37°C with 5% CO2 for 2 hours before the culture medium was replaced with growth medium (Neurobasal A (Thermo Fisher, 12349015) supplemented with 1 x B27 Plus (Thermo Fisher ...
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bioRxiv - Immunology 2023Quote: To analyze proliferation in vitro, B cells (2 x 106, purified as above) were labeled with 5 µM CellTrace Violet (CTV) (Invitrogen, Waltham, MA) and then activated and cultured as above ...
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Activation of glucocorticoid receptor signaling inhibits KSHV-induced inflammation and tumorigenesisbioRxiv - Cancer Biology 2023Quote: ... Cell cycle was examined by flow cytometry following 5′-bromo-2′-deoxyuridine (BrdU) labeling (BrdU: Sigma-Aldrich, MB5002; BrdU antibody: Invitrogen, B35129) and propidium iodide (PI ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Peptides were trapped on an Acclaim Pepmap 100 C18 trap column (100 µm x 2 cm, particle size 5 µm, Thermo Fisher Scientific) and separated on an in-house packed analytical column (35 cm x 75 µm ...
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bioRxiv - Developmental Biology 2023Quote: ... three T175 flasks of Ishikawa cells at 60-70% confluence were supplemented with 1 µM 5-ethynyl-2’-deoxyuridine (EdU, Thermo Fisher Scientific) and incubated overnight to allow EdU incorporation into the DNA ...
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bioRxiv - Immunology 2024Quote: ... Sample were loaded at 10 μL/min onto a trap column (100 μm × 2 cm, PepMap nanoViper C18 column, 5 μm, 100 Å, Thermo Scientific) equilibrated in 0.1% trifluoroacetic acid (TFA) ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were then incubated in freshly prepared blocking buffer (10% goat serum/1X PBS/0.2% TritonX-100) for an hour at room temperature before incubating in Alexa Fluor 488 conjugated anti-Claudin-5 (ThermoFisher #4C3C2, #352588), diluted in blocking reagent (1:100 dilution ...
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bioRxiv - Immunology 2024Quote: ... Sample were loaded at 10 μL/min onto a trap column (100 μm × 2 cm, PepMap nanoViper C18 column, 5 μm, 100 Å, Thermo Scientific) equilibrated in 0.1% trifluoroacetic acid (TFA) ...
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bioRxiv - Immunology 2024Quote: ... 1.5 μg aliquot of each sample was loaded at 15 μL/min onto a trap column (100 μm × 2 cm, PepMap nanoViper C18 column, 5 μm, 100 Å, Thermo Scientific) equilibrated in 0.1% trifluoroacetic acid (TFA) ...
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bioRxiv - Developmental Biology 2024Quote: ... Phase separation in cells lysed in TRIzol was induced with 50% isopropanol and 0.5% 2-mercaptoethanol and RNA was extracted from the aqueous phase using the MagMAX mirVana Total RNA Isolation Kit (Thermo Fisher, A27828) on a KingFisher Flex Magnetic Particle Processor according to the manufacturer’s protocol with 1-2 million cells input ...
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bioRxiv - Neuroscience 2024Quote: Whole brains were extracted from euthanized mice (5% isoflurane exposure followed by cervical dislocation) and incubated for 5 minutes on ice in cellular stabilizing solution: 45% HEBG medium (2% 50X B27 (Thermo Fisher; 17504044) and 0.25% 100X Glutamax (Thermo Fisher ...
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Structure, dynamics and evolution of the Candida albicans multi-drug resistance ABC transporter CDR1bioRxiv - Microbiology 2024Quote: ... The grid was blotted for 2 seconds at 5°C and 100% humidity using a Vitrobot Mark IV (Thermo Fisher Scientific, USA). A total of 3650 movies were collected by SerialEM software46 under counting mode at a magnification of 100,000× (pixel size 1.25Å ...
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bioRxiv - Immunology 2024Quote: ... Brain areas were individually Dounce homogenized in 6ml ice cold FACS buffer (5% Bovine Serum Albumin Proteins, 2 mM Ethylenediaminetetraacetic Acid (EDTA) in PBS (Gibco 14190-144), sterile filtered ...
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bioRxiv - Physiology 2024Quote: ... the cell suspension was centrifuged at 300xg for 5 minutes before being resuspended in a pre-warmed neurobasal medium supplemented with 2% B27 supplement (Gibco Invitrogen, CA, USA), 0.25% GlutaMAX (Gibco Invitrogen ...
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bioRxiv - Plant Biology 2024Quote: ... Gels were semi-dry transferred to PVDF membranes and then blocked with 5% non-fat milk in Tris-buffered saline with Tween (TBST) (iblot 2, Thermo Fisher Scientific). All antibodies were diluted in 1:2,000 in TBST with 5% milk ...