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Citations for Thermo Fisher :
6751 - 6800 of 10000+ citations for 2 3 5 6 Tetrahydroxy 4 phosphonooxycyclohexyl dihydrogen phosphate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... The cDNA was then diluted 20 times and 5 µl of the dilution was mixed with 2×VeriQuest Fast SYBR Green qPCR Master Mix (Affymetrix, 75690) and primers for the real-time qPCR analysis ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were loaded on pre-column (C18, 5 µm, 100 A°, 100 µ, 2 cm Nano-viper column # 164564, Thermo Scientific) at 5 µl/min flow rate for about 10 minutes and then peptides were separated on a 50 cm column (C18 ...
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bioRxiv - Physiology 2023Quote: ... Real-time PCR was performed using RealQ Plus 2× Master Mix Green (Ampliqon, #A324402) on a QuantStudio 5 machine (Applied Biosystems). Expression was calculated using the delta-delta-Ct method ...
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bioRxiv - Developmental Biology 2023Quote: ... NAE staining was followed by 5-ethynyl-2⍰-deoxyuridine (EdU) detection using Click-iT Edu Alexa Fluor 488 or 594 Imaging kits (Life Technologies) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... Samples were then resuspended in 300 μl PBS + 2% FBS together with 5 μl CountBright™ Absolute Counting Beads (ThermoFisher Scientific) and run on a 5-laser BD Fortessa (BD Biosciences) ...
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bioRxiv - Cell Biology 2023Quote: ... Biotinylation and streptavidin-bead binding were performed as above with the exception that in this case the flowthrough was discarded and bead-bound nascent RNA was eluted by incubating the beads at 2 x 5 min at 65C in 100mM TCEP (Thermo Scientific), 10mM EDTA ...
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bioRxiv - Synthetic Biology 2022Quote: ... a 1:10 dilution was performed and 2 μl of diluted DNA was amplified by means of a QuantStudio 5 (Applied Biosystems) thermal cycler using the SYgreen Blue Mix (PCRbio ...
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bioRxiv - Molecular Biology 2022Quote: ... Five microliters of sample were loaded onto the trapping column (Acclaim PepMap RSLC, 100 μm × 2 cm, nanoViper, 5 μm 100Å C 18 particles, Thermo Scientific) using 0.1% acetic acid at a flow rate of 7000 nl/min for 3 minutes at RT ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were washed with 2× saline sodium citrate (SSC) buffer for 5⍰min and blocked with UltraPure Salmon Sperm DNA (Thermo Fisher) in QuikHyb Hybridization Solution (Agilent Technologies ...
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bioRxiv - Microbiology 2022Quote: ... the robot adds 2 volumes of 30% PEG 6000 (in 1.6 M NaCl) and 35ul of 1:5 diluted Glycoblue (Invitrogen, Thermo Fisher) to each well ...
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bioRxiv - Cell Biology 2024Quote: ... Two sets of 12 100 µL 2.66 ng/ µL enriched resuspended mitochondria aliquots in 200 µL PCR tubes were made per condition and heated on a temperature gradient of 42-64°C (2°C steps) for 5 minutes at each temperature using a Veriti 96-well thermal cycler (Applied Biosystems).
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bioRxiv - Cell Biology 2024Quote: All experiments were carried out in HeLa cells ATCC CCL-2 (ECACC General Collection) grown in a humidified incubator at 37°C with 5% CO2 in DMEM (Gibco-ThermoFisher) supplemented with 10% fetal bovine serum (Corning ...
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bioRxiv - Bioengineering 2023Quote: ... mRNA was co-transcriptionally capped using m7(3’OMeG)(5’)ppp(5’)(2’OMeA)pG capping reagent (Hongene Biotech) in a “one-pot” reaction followed by digestion with DNase I (Thermo Fisher). Linear mRNA was purified using Dynabeads MyOne Carboxylic Acid beads (Thermo Fisher).
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bioRxiv - Cell Biology 2024Quote: ... Samples were loaded at 10 μL/min onto a trap column (100 μm × 2 cm, PepMap nanoViper C18 column, 5 μm, 100 Å, ThermoFisher) equilibrated in 0.1% trifluoroacetic acid (TFA) ...
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bioRxiv - Microbiology 2024Quote: ... Nuclei were labelled with a PBS-Hoechst solution at 2 μg/mL for 5 min at RT and samples were covered in mounting medium (ProLong P36930, Thermofisher scientific). Labelled cells were analysed with an inverted LSM 980 confocal microscope with Airyscan 8Y (Carl Zeiss Ltd.) ...
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bioRxiv - Pathology 2024Quote: ... 2 µl drops of yeast suspension were plated in five 1:5 serial dilutions on 100 mm square plates (Thermo Fisher) with selective SD medium and increasing concentrations (0.07–7 µg/ml ...
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bioRxiv - Molecular Biology 2024Quote: ... Digestions were incubated for 1 hour at 37 ℃ and then diluted 1:5 with UltraPure water before loading on E-gel EX 2% Agarose gels (Invitrogen, G401002). Backbones were purified using the Monarch Gel Extraction Kit (Monarch) ...
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bioRxiv - Immunology 2024Quote: ... The cDNA generated was then diluted 2-5 fold in RNase- and DNase-free water and mixed with PowerUP SYBR Green Master Mix (Applied Biosystems) and primers (listed in Supplemental Table 3 ...
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bioRxiv - Cancer Biology 2024Quote: ... TRACER rolls and unrolled control samples were incubated at 37 °C in complete organoid media with 50 μM of 5-Iodo-2’deoxyuridine (IdU, Fisher Scientific) and 20 uM of Telox64 (courtesy of Mark Nitz ...
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bioRxiv - Neuroscience 2023Quote: ... Two larvae at a time were cold anesthetized on ice in a custom recording chamber for 5 minutes and immobilized dorsal side up in 2% low melting point agarose (BP1260-100, Fisher Scientific). Recording chambers were placed on the stage of an upright microscope (Olympus BX-51W ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were washed in PBS (2 x 5 min) and incubated in 0.1% Tween in PBS containing streptavidin Alexa 594 conjugate (ThermoFisher, 1:1500) for 2 hours at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Peptides were desalted on-line using a capillary trap column (Acclaim Pepmap100, 100 μm, 75 μm × 2 cm, C18, 5 μm (Thermo Scientific)) and then separated using a 180 min RP gradient (4–30% acetonitrile/0.1% formic acid ...
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bioRxiv - Bioengineering 2023Quote: Proliferative activity of 2D hydrogel cell cultures was measured using an EdU (5-ethynyl-2’-deoxyuridine) labeling kit (Click-iT™ EdU Cell Proliferation Kit for Imaging, Invitrogen). 5 μM EdU solution was dosed to cells 12 h prior to fixing with 4% paraformaldehyde ...
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bioRxiv - Cancer Biology 2023Quote: ... SCC25-Luc-GFP cells co-cultured with mouse or naked mole-rat fibroblasts were incubated with 10 µM 5-ethynyl-2’-deoxyuridine (EdU) (Thermo Fisher) for 1.5 hours ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Amoebae were grown from frozen spores at room temperature on 100 x 15mm plates filled with 30mL SM/5 agar (2 g glucose (Fisher Scientific), 2 g Bacto Peptone (Oxoid) ...
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bioRxiv - Neuroscience 2023Quote: ... tissues were placed in a digestion solution containing HBSS-glucose and 5 mM CaCl2 (Cat. #: 1564A930, Fischer Scientific) and a combination of collagenase II (2 mg/ml, Cat. #: 17101-015, Life Technologies) and dispase (5 mg/ml ...
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Pathogenic CD8 T cell responses are driven by neutrophil-mediated hypoxia in cutaneous leishmaniasisbioRxiv - Immunology 2023Quote: ... 20 U/mL recombinant human IL-2 at 37°C and 5% CO2 for 24 hours in RPMI 1640 (Gibco, Canada) containing 100 Units of penicillin and 0.1 mg/mL of Streptomycin (Sigma ...
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bioRxiv - Pathology 2024Quote: ... The samples were loaded onto in Acclaim PepMap 100 trap column (100 μm x 2 cm, nanoViper, C18, 5 μm, 100Å; Thermo Scientific) and separated on an Acclaim PepMap RSLC (75 μm x 50 cm ...
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bioRxiv - Systems Biology 2024Quote: ... comprising a Acclaim PepMap 100 trapping column (2 cm x 100 µm i.d., 5 µm 100 Å C18, Thermo Fisher Scientific) and a consecutive nanoEase M/Z Peptide BEH analytical column (25 cm x 75 µm i.d. ...
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bioRxiv - Bioengineering 2022Quote: Cell proliferation in T-SLICE zones was analyzed using an EdU (5-ethynyl-2’-deoxyuridine) Cell Proliferation Kit (Click-iT− EdU Cell Proliferation Kit for Imaging, Alexa Fluor™ 488 dye: Invitrogen). Cell culture media was supplemented throughout with 10 uM EdU ...
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bioRxiv - Cancer Biology 2022Quote: ... newly synthesized DNA was labeled using the Click-iT Plus 5-ethynyl-2’-deoxyuridine (EdU) Alexa Fluor 647 flow cytometry assay kit (ThermoFisher Scientific) according to manufacturer ‘s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... peptides were pre-concentrated with 0.1% TFA on a trap column (AcclaimR PepMap 100 column, 100 μm i.d. × 2 cm, C18, 5 μm particle size, 100 Å pore size; Thermo Scientific) at a flow rate of 7 μL/min for 10 min ...
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bioRxiv - Plant Biology 2023Quote: ... the media was aspirated from the cells and replaced with the Fura2-loading solution (5 µM Fura-2-AM and 0.15% Pluronic F127 (Invitrogen, Waltham, USA) dissolved in D-MEM/Ham’s F-12 with 10 %FBS) ...
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bioRxiv - Biochemistry 2023Quote: ... The temperature was increased from 5 °C to 95 °C over 2 hours and readings taken using a One Step Plus RT-PCR (Applied Biosystems).
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bioRxiv - Plant Biology 2023Quote: ... Peptides were trapped to a reverse phase trap column (Acclaim PepMap, C18 5 µm, 100 µm x 2 cm, Thermo Scientific) connected to an analytical column (Acclaim PepMap 100 ...
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bioRxiv - Cell Biology 2023Quote: ... From day 0 to day 5 the medium was changed daily with 2 mL DMEM/F-12 containing 20% KnockOut™ Serum (ThermoFisher), 1 x Non Essential Amino Acid (NEAA ...
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bioRxiv - Cancer Biology 2024Quote: ... 100% EtOH 2 min) and xylene (two 5 min incubations) and mounted using Fisher Chemical Permount Mounting Medium (Fisher Scientific SP15) and microscope cover glass (Fisher Scientific 12545F) ...
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bioRxiv - Plant Biology 2024Quote: In situ hybridization experiments were performed on 2 - 5 mm ear primordia and 10-21 day old seedling apexes fixed in PFA (Thermo Scientific), dehydrated in a graded ethanol series ...
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bioRxiv - Neuroscience 2024Quote: ... the electrodes were filled with 2 µl of 5 mM of Alexa Fluor 568 hydrazide (Invitrogen, diluted in 200 mM KCl) and backfilled with 10 µl of 200 mM KCl ...
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bioRxiv - Biochemistry 2024Quote: ... Dried cell lysates were digested using 2 μL of a digestion solution containing 5 ng/μL LCMS grade trypsin (Pierce) in 0.1% DDM (Thermo Fisher, 89902) and 50mM TEAB (Thermo Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... MDA-MB-231 and HEK-293T RNAs (5 μg) were incubated with 2 U TURBO DNase for 30 min at 37 °C (Thermo Fisher). After DNase digestion ...
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bioRxiv - Cell Biology 2024Quote: ... Cells obtained from fractions 2–5 were combined according to the genotype and expanded in α-MEM containing 10% FBS (Gibco), 100 U/ml penicillin ...
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bioRxiv - Genomics 2024Quote: ... A small piece of renal CTX tissue (approx. 2 mm) and the renal IM were transferred to 5 ml ice-cold PBS (Thermo Scientific). After centrifugation at 500 x g for 5 min at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... qRT‒PCR was performed using 2× RealStar Power SYBR qPCR Mix (Genstar #A304) in a QuantStudio 5 Real Time PCR system (Applied Biosystems). cDNA was synthesized using a StarScript III RT Kit (Genstar #A232) ...
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bioRxiv - Molecular Biology 2024Quote: Differentiated hiPSCs-derived control myotubes or human primary control myoblasts were transfected at day 5 of differentiation with 50 nM 2-OMe ASOs using RNAi Max transfection reagent (Life Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... 1 µl of the sample was loaded on an Acclaim PepMap 100 trap column (100 µm × 2 cm, C18 reversed phase, particle size: 5 µm; Thermo Scientific) using a flow rate of 7 µL/min ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then washed in TSB-T 2 x 5 min and rinsed with TBS before mounting with ProLongTM Diamond Antifade Mountant (Invitrogen, #P36961). Following staining with secondary antibodies ...
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bioRxiv - Immunology 2024Quote: ... 50 μL lysate (∼25 ug total protein) was mixed with 2 μg 5’-biotinylated dsDNA probe and 50 μL streptavidin agarose resin (Thermo Scientific) in PBS containing protease inhibitors (Roche ...
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bioRxiv - Cell Biology 2024Quote: ... was added per well and incubated for 2-5 minutes before adding 100 µL the ELISA stop solution (Thermo Fisher Scientific). Absorbance was measured on the Varioskan microplate reader at 450 nm (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2024Quote: ... the nucleus was counterstained with 0.5 µg/mL 4’6-diamidino-2-phenylindole (DAPI) for 10 minutes and slides were mounted with Prolong® Gold (Life Technologies).