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Citations for Thermo Fisher :
7151 - 7200 of 10000+ citations for 2 3 5 6 Tetrahydroxy 4 phosphonooxycyclohexyl dihydrogen phosphate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Samples were loaded at 10 μl/min onto a trap column (100 μm × 2 cm, PepMap nanoViper C18 column, 5 μm, 100 Å, Thermo Scientific) equilibrated in 0.1% trifluoroacetic acid (TFA) ...
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bioRxiv - Immunology 2024Quote: ... Brain areas were individually Dounce homogenized in 6ml ice cold FACS buffer (5% Bovine Serum Albumin Proteins, 2 mM Ethylenediaminetetraacetic Acid (EDTA) in PBS (Gibco 14190-144), sterile filtered ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were then incubated in freshly prepared blocking buffer (10% goat serum/1X PBS/0.2% TritonX-100) for an hour at room temperature before incubating in Alexa Fluor 488 conjugated anti-Claudin-5 (ThermoFisher #4C3C2, #352588), diluted in blocking reagent (1:100 dilution ...
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bioRxiv - Immunology 2024Quote: ... Sample were loaded at 10 μL/min onto a trap column (100 μm × 2 cm, PepMap nanoViper C18 column, 5 μm, 100 Å, Thermo Scientific) equilibrated in 0.1% trifluoroacetic acid (TFA) ...
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bioRxiv - Plant Biology 2024Quote: ... Gels were semi-dry transferred to PVDF membranes and then blocked with 5% non-fat milk in Tris-buffered saline with Tween (TBST) (iblot 2, Thermo Fisher Scientific). All antibodies were diluted in 1:2,000 in TBST with 5% milk ...
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bioRxiv - Physiology 2024Quote: ... the cell suspension was centrifuged at 300xg for 5 minutes before being resuspended in a pre-warmed neurobasal medium supplemented with 2% B27 supplement (Gibco Invitrogen, CA, USA), 0.25% GlutaMAX (Gibco Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... Peptides were trapped on an Acclaim Pepmap 100 C18 trap column (100 μm x 2 cm, particle size 5 μm, Thermo Fisher Scientific) and separated on an in-house packed analytical column (75 μm x 30 cm ...
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bioRxiv - Plant Biology 2024Quote: ... 7.5 % (v/v) glycerol and 0.01 % (w/v) bromophenol blue) containing 2 mM of thiol-modifying reagent AMS (Invitrogen, Cat. No. A485). After labeling for 1 h at 25 °C in the dark ...
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bioRxiv - Microbiology 2024Quote: Electrocompetent B31-e2 or 297 cells were transformed by electroporation (37) with 500 ng to 5 μg of plasmid in 2-mm gap cuvettes (Thermo Scientific; 5520) using a Bio-Rad MicroPulser on EC2 setting ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were exposed to the medium supplemented with 100 nM HaloTag TMR ligand and 10 µM 5-ethynyl-2′-deoxyuridine (EdU) (C10337; Thermo Fisher Scientific) for 30 min immediately after washing out 7BRO ...
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bioRxiv - Cancer Biology 2024Quote: ... an equivalent of 1 µg of each fraction was loaded at 15 μL/min onto a trap column (100 μm × 2 cm, PepMap nanoViper C18 column, 5 μm, 100 Å, Thermo Scientific) equilibrated in 0.1% TFA ...
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bioRxiv - Cancer Biology 2024Quote: Cell proliferation in response to GNE-3511 treatment was assessed by 5-ethynyl-2′-deoxyuridine (EdU) incorporation using the Click-iT EdU Alexa Fluor 488 cell proliferation assay kit (Invitrogen, Carlsbad, CA). CAL33 cells were seeded in µ-Plate 24 Well plates (Ibidi GmbH ...
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bioRxiv - Cancer Biology 2024Quote: ... A volume of 6 µL for tumor glycopeptide samples or 1-6 µL for PHEA-enriched fractions was injected onto an Acclaim PepMap 100 column packed with 2 cm of 5 µm C18 material (Thermo Fisher, 164564) using 0.1% FA in water (solvent A) ...
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bioRxiv - Cell Biology 2024Quote: ... 50 uL of TCM + 40U/mL of IL-2 was added to one side of the channel and 50uL of TCM + 40 U/mL of IL-2 + 5-10ug/mL of 10kDa AF647-Dextran (Invitrogen, Cat#: D22914) + 200nM recombinant human CCL19 (Biotechne ...
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bioRxiv - Developmental Biology 2024Quote: ... Cell dissociation was stopped by adding an equal volume of STOP solution (5% FBS, 2% BSA in DMEM)(Corning 35-010-CV/Millipore Sigma A9418-5G/Gibco A1896701) and gently inverting the tube ...
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bioRxiv - Cell Biology 2024Quote: The proliferation rate was assessed by quantifying 5-ethynyl-2’-deoxyuridine (EdU) cellular incorporation using the Click-iTTM EdU Alexa FluorTM 488 Imaging kit (Thermo Fisher Scientific). A number of 4.0x104 ECFCs were plated on collagen-coated flasks 1.86 cm2 surface area ...
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bioRxiv - Bioengineering 2023Quote: ... stained with 5 ng/mL dichlorotriazinylaminofluorescein (5-DTAF, Invitrogen), and mounted in custom grips at a 10 mm gauge length ...
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bioRxiv - Cell Biology 2024Quote: ... 5% CO2 and 5% O2 in RPMI 1640 (Gibco) with 15% FBS (Gibco ...
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bioRxiv - Cell Biology 2021Quote: Human LAMP2A was synthesized into pUC57 Plasmid with the sequence: (5’-CAAGTTTGTACAAAAAAGCAGGCTCTCGAGCAatgGTGTGCTTCCGCCTCTTCCCGGTTCCGGGCTCAGGGCTCGTTCTGGTCTGCCTAGTCCTGGGAGCTGTGCGGTCTTATGCATTGGAACTTAATTTGACAGATTCAGAAAATGCCACTTGCCTTTATGCAAAATGGCAGATGAATTTCACAGTACGCTATGAAACTACAAATAAAACTTATAAAACTGTAACCATTTCAGACCATGGCACTGTGACATATAATGGAAGCATTTGTGGGGATGATCAGAATGGTCCCAAAATAGCAGTGCAGTTCGGACCTGGCTTTTCCTGGATTGCGAATTTTACCAAGGCAGCATCTACTTATTCAATTGACAGCGTCTCATTTTCCTACAACACTGGTGATAACACAACATTTCCTGATGCTGAAGATAAAGGAATTCTTACTGTTGATGAACTTTTGGCCATCAGAATTCCATTGAATGACCTTTTTAGATGCAATAGTTTATCAACTTTGGAAAAGAATGATGTTGTCCAACACTACTGGGATGTTCTTGTACAAGCTTTTGTCCAAAATGGCACAGTGAGCACAAATGAGTTCCTGTGTGATAAAGACAAAACTTCAACAGTGGCACCCACCATACACACCACTGTGCCATCTCCTACTACAACACCTACTCCAAAGGAAAAACCAGAAGCTGGAACCTATTCAGTTAATAATGGCAATGATACTTGTCTGCTGGCTACCATGGGGCTGCAGCTGAACATCACTCAGGATAAGGTTGCTTCAGTTATTAACATCAACCCCAATACAACTCACTCCACAGGCAGCTGCCGTTCTCACACTGCTCTACTTAGACTCAATAGCAGCACCATTAAGTATCTAGACTTTGTCTTTGCTGTGAAAAATGAAAACCGATTTTATCTGAAGGAAGTGAACATCAGCATGTATTTGGTTAATGGCTCCGTTTTCAGCATTGCAAATAACAATCTCAGCTACTGGGATGCCCCCCTGGGAAGTTCTTATATGTGCAACAAAGAGCAGACTGTTTCAGTGTCTGGAGCATTTCAGATAAATACCTTTGATCTAAGGGTTCAGCCTTTCAATGTGACACAAGGAAAGTATTCTACAGCTCAAGACTGCAGTGCAGATGACGACAACTTCCTTGTGCCCATAGCGGTGGGAGCTGCCTTGGCAGGAGTACTTATTCTAGTGTTGCTGGCTTATTTTATTGGTCTCAAGCACCATCATGCTGGATATGAGCAATTTTAGGGTACCACCCAGCTTTCTTGTACAAAGTGGACGCGT-3’) Human LAMP2A was subcloned into pLenti6 (Thermo Scientific), a Gateway adapted vector using BP/LR Clonase II (Thermo Scientific) ...
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bioRxiv - Systems Biology 2023Quote: ... and washed again in PBS (3 x 5 min at RT) before being mounted in Prolong Gold (Invitrogen by Thermo Fisher Scientific, Grand Island, NY, USA) and allowed to cure for 48 hours at RT before imaging ...
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bioRxiv - Biophysics 2021Quote: ... The coverslips were then rinsed three times with phosphate-buffered saline (PBS; Thermo Fisher Scientific, Germany) and stored at 4 °C overnight.
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bioRxiv - Cancer Biology 2021Quote: ... 10 mM Sodium phosphate (pH 7.2 at 25°C) and 0.2 U/µl RNase T1 (ThermoFisher). Reaction mixtures were incubated at 24°C for 10 min or at 37°C for 4 min ...
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bioRxiv - Biochemistry 2020Quote: ... HAP1 cells and fibroblasts were washed twice in ice-cold Dulbecco’s Phosphate-Buffered Saline (DPBS, Gibco). The second PBS wash contained the protease inhibitors (0.23 mM PMSF and 0.64 mM Benzamidine) ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were blocked in 0.1% Tween20 in Phosphate buffered saline (PBS) starting block (Thermo Fisher 37538) and incubated with primary antibodies diluted in the same buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... retinas were homogenized in cold lysis buffer (1X phosphate-buffered saline pH 7.4 (Thermo Fisher Scientific), Pierce® protease inhibitor ...
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bioRxiv - Genomics 2020Quote: ... Samples were then resuspended in phosphate buffered saline with 0.04% bovine serum albumin (Thermo Fisher Scientific) at 1⎕×⎕106 cells per ml.
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Definition and Signatures of Lung Fibroblast Populations in Development and Fibrosis in Mice and MenbioRxiv - Cell Biology 2020Quote: ... In adult mice the lungs were cleared of blood by flushing phosphate buffered saline (#10010023, Gibco, Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... S protein binding was detected using 25 µL/well of PNPP (p-nitrophenyl phosphate;Thermo Fisher) and the reaction was measured at a wavelength of 405 nm by the Varioskan Lux Reader (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2021Quote: ATP hydrolysis was analysed in steady state reactions using an ENZCheck Phosphate Assay Kit (Life Technologies). 150 μL samples containing standard reaction buffer supplemented with 2 mM of ATP were assayed in a 18 BMG Labtech PherAstar FS plate reader at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... and pellets were washed with sterile 1X phosphate buffered saline (PBS, Thermo Fisher Scientific, Emeryville, CA) three times prior to creating glycerol stocks that were frozen for later use ...
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bioRxiv - Bioengineering 2021Quote: ... Excess collagen solution was flushed out of the chamber with phosphate-buffered saline (PBS pH7.4, Invitrogen), and the device was UV-sterilized for 10 min ...
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bioRxiv - Genomics 2021Quote: ... Each worm was rinsed with 1 mL of PBS (Phosphate Buffered Saline, pH 7.4 Life Technologies) to remove fish fluids and then immediately put in a 2 mL tube of PBS ...
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bioRxiv - Neuroscience 2020Quote: ... 12.5 μg of total protein was diluted in Dulbecco’s phosphate-buffered saline (DPBS) (Gibco, Waltham, USA) with 2% SDS and 50 mM DTT and heat denatured at 95° C for 5 min ...
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bioRxiv - Microbiology 2020Quote: ... washed twice with 1x Dulbecco’s phosphate-buffered saline (DPBS; MT-21-030-CV, Thermo Fisher Scientific) supplemented with 5% Tween80 (v/v) ...
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bioRxiv - Microbiology 2021Quote: ... The cecal samples were dissolved in sterile phosphate-buffered saline (PBS; Life Technologies, Carlsbad, CA, USA) at a concentration of 1 g per 5 mL ...
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bioRxiv - Neuroscience 2020Quote: ... they were washed twice with 1x Dulbecco’s phosphate-buffered saline (dPBS; Thermo Fisher Scientific, Waltham, MA) and with serum free medium (SFM ...
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bioRxiv - Biophysics 2020Quote: ... washed three times in phosphate buffer solution (PBS, with no magnesium or calcium added, Thermo Scientific), and resuspended in homogenization buffer (0.5mM MgCl2 ...
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bioRxiv - Cancer Biology 2021Quote: ... cells gently washed with Dulbecco’s Phosphate Buffered Saline (PBS; without magnesium and calcium; Thermo Fisher #14190169), and differentiation induced by adding basic differentiation medium (BDM ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were blocked in 0.1% Tween20 in Phosphate buffered saline (PBS) starting block (Thermo Fisher 37538) and incubated with primary antibodies diluted in the same buffer ...
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bioRxiv - Biochemistry 2022Quote: ... HeLa cells were washed three times with Dulbecco’s Phosphate Buffered Saline (PBS) (Gibco Cat# 14190-094) and then incubated for 1 h in Earle’s Balanced Salt Solution (EBSS ...
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bioRxiv - Molecular Biology 2022Quote: ... washed with 1× phosphate buffered saline (PBS) and lysed with RIPA buffer (Thermo Scientific™, 89900) containing protease inhibitors ...
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bioRxiv - Genomics 2022Quote: ... the collected cells were resuspended in UV-treated Dulbecco’s phosphate-buffered saline (-) (DPBS, Thermo Fisher Scientific) and washed three times with DPBS ...
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bioRxiv - Bioengineering 2022Quote: Samples were fixed with 10% phosphate buffered formalin (Catalog No. SF100, Thermo Fisher Scientific, Waltham, MA) and embedded in paraffin following a series of graded ethanol and xylene incubations ...
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bioRxiv - Developmental Biology 2022Quote: ... Sections were rinsed with phosphate buffer and then mounted and cover-slipped with Fluoromount-G (ThermoFisher). For spinal cord analysis ...
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bioRxiv - Microbiology 2021Quote: ... Plates were then washed again and 100 μl p-nitrophenyl phosphate substate (Thermo Scientific Cat. 37620) was added and the plate was developed for 30 min at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells at 70-80% confluency were rinsed in PBS (1x Phosphate-buffered saline, Gibco, #14190-144) and trypsinised in Trypsin-EDTA (0.05% ...
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bioRxiv - Genomics 2020Quote: ... Spleens were mechanically dissociated into single cells suspensions in cold phosphate-buffered saline (PBS; Gibco: 10010023)/10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2020Quote: ... and the apical surface was washed with 1x Dulbecco’s phosphate-buffered saline (DPBS) (Thermo Fisher Scientific) once per week initially but more frequently when more mucus was observed on later days ...
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bioRxiv - Neuroscience 2021Quote: ... and transcardially perfused with 25 ml of 0.125 M phosphate buffered saline (PBS, pH 7.4, Gibco) and 40 ml of 4% paraformaldehyde (PFA ...
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bioRxiv - Immunology 2022Quote: ... washed 3x with wash buffer (KPL 10X Phosphate Buffered Saline with 0.05% Tween 20 (Fisher Scientific)) and blocked with Superblock (ScyTek ...