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Citations for Thermo Fisher :
6801 - 6850 of 10000+ citations for 2 3 5 6 Tetrahydroxy 4 phosphonooxycyclohexyl dihydrogen phosphate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... 400,000 cells were stained with 2 mL of 5 µM C11-Bodipy 581/591 fluorescent probe in Live Imaging Solution (Thermo Fisher) or left unstained (unstained control group) ...
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bioRxiv - Neuroscience 2024Quote: 5-ethynyl-2’-deoxyuridine (EdU)-incorporation assay was performed using Click-iT Plus EdU Alexa Fluor 488 Imaging Kit (Invitrogen, C10637). Animals were injected intraperitoneally with EdU reagent at a dose of 10 µl per 1 g animal (stock solution 20 mM solution in saline) ...
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bioRxiv - Synthetic Biology 2024Quote: ... The sample was then dialyzed for 5 hours in 10x PBS pH 8.4 buffer with a 2 kDa membrane (Fisher Scientific, #15310692) and quantified with a NanoDrop ...
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bioRxiv - Physiology 2024Quote: Detached corneal epithelial sheets and dissociated cells were placed in the recording chamber (Warner Instruments (Hamden, CT, USA)) and loaded with Fura-2 AM (5-10 µM, Life Technologies) for 30 to 45 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... IP samples used for the broad peptide screen in Figure 2 were prepared using 100 mg HEK293TLD / pLD401 powder and 5 µl (slurry) Dynabeads M-270 Epoxy (ThermoFisher Scientific) conjugated with α-ORF2p cl ...
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bioRxiv - Molecular Biology 2024Quote: ... Trapping was performed at 20 μl/min for 2 min in loading solvent A on a 5 mm trapping column (Thermo scientific, Pepmap ...
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bioRxiv - Neuroscience 2024Quote: ... sections were washed 2×5 min in TBS and cover-slipped with ProLong Diamond mounting media 275 containing DAPI (#P36962, ThermoFisher Scientific).
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bioRxiv - Biochemistry 2024Quote: Cells were treated as described in the figure legends and 1 hour prior to harvest incubated with 10μM 5-ethynyl-2-deoxyuridine (EdU; Click-iT EdU Flow Cytometry Kit, ThermoFisher, Loughborough, UK). Cells were harvested by trypsinisation and fixed with 4% paraformaldehyde/PBS for 10 min at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... cells were treated as described in the figure legends and 1 hour prior to harvest incubated with 10μM 5-ethynyl-2-deoxyuridine (EdU; Click-iT EdU HCS Kit, ThermoFisher, Loughborough, UK). Fixed and processed as above and EdU detected according to the manufacturer’s protocol.
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bioRxiv - Biochemistry 2024Quote: ... cells were beadloaded with 6 µL of 5 µM probe and 100 nM HaloTag-JF669 ligand and 2 drops of NucBlue live cell stain (Life Technologies) were added at the time of beadloading to stain the HaloTag-G3BP1 protein and illuminate the nucleus ...
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bioRxiv - Bioengineering 2024Quote: ... and resuspended at 5×106 cells/mL in 1× PBS containing 2 µM CellTrace Violet (CTV, Thermo Fisher, USA Cat#C34571). Cells were incubated at 37°C (protected from light ...
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bioRxiv - Cancer Biology 2024Quote: 293FT cells cultured in antibiotic-free media (DMEM, 10% FBS, 2 mM glutamine) were transfected in 5% Lipofectamine™ 2000 (Invitrogen) using packaging plasmids Δ8.9 ...
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bioRxiv - Bioengineering 2024Quote: ... media containing lentivirus was replaced with fresh media and cells were allowed to recover for another 24 hours before starting selection with 2 μg/mL puromycin for 5 days (Gibco, A1113803).
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bioRxiv - Cell Biology 2024Quote: ... 2 μL of 5 fold diluted cDNA product was subjected to RT-qPCR in StepOnePlus Real-Time PCR System (Applied Biosystems) using SYBR Green PCR Master Mix reagent as the detector ...
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bioRxiv - Biochemistry 2024Quote: ... Peptides were trapped at 20 μL/min in a loading solvent (2% ACN, 0.05% trifluoroacetic acid) on a 5 mm × 300 μm C18 PepMap cartridge pre-column (Thermo Fisher Scientific) for 5 min ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were blocked with TBST + 5% nonfat dry milk then immunoblotted with anti-alpha-actinin 2 (Invitrogen EA-53; mouse monoclonal) and anti-actin (GeneTex HL1926 ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA was diluted 1:5 in nuclease-free water and 2 µL was mixed with 10 µL 2X SYBR Select MasterMix (ThermoFisher 4472908) and 250 nM forward and reverse qPCR primers in a 20 µL final volume per sample ...
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bioRxiv - Microbiology 2021Quote: ... 5% human serum and 5% AlbuMAX-II (Gibco)) was replaced daily until reaching maturity at day 14-post induction ...
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bioRxiv - Immunology 2023Quote: ... 2mg of 5-ethynyl uridine (5-EU, Thermofisher) was injected intraperitoneally at D12 following SRBC immunisation and similar preparation and labelling steps described for the ex vivo 5-EU assay were followed ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed with PBS and incubated overnight at 4°C with primary antibody solution: Antibody in IF buffer containing 3% Saponin (Fisher Scientific, Cat. No. 55-825-5100GM). Antibodies used were as follows ...
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bioRxiv - Developmental Biology 2021Quote: ... Quantitative PCR (QuantStudio 6, Applied Biosystems, Waltham, MA) was used to measure gene expression levels and normalized to the housekeeping gene hypoxanthine guanine phosphoribosyl transferase (Hprt) ...
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bioRxiv - Cell Biology 2022Quote: ... on a QuantStudio 6 Flex instrument (Applied Biosystems) in a 96 well format ...
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bioRxiv - Immunology 2020Quote: ... using a Quant Studio Flex 6 (Applied Biosystems). Samples were run in triplicate wells in a 96-well or 384 well plate ...
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bioRxiv - Bioengineering 2021Quote: ... media was changed to Essential 6 media (ThermoFisher) and was replaced daily for 4 days ...
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bioRxiv - Bioengineering 2021Quote: ... 6 mL FACS Rinse (Thermo Fisher, BD 340346), 6 mL 80% ethanol ...
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bioRxiv - Bioengineering 2020Quote: ... 6-well culture plates (Corning; Thermo Fisher Scientific), and overlaid with glass coverslips to aid in tissue adherence ...
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bioRxiv - Biophysics 2021Quote: ... 6 mM L-glutamine (Thermo Fisher Scientific, USA), 0.2 % (v/v ...
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bioRxiv - Plant Biology 2021Quote: ... Gel (Novex TBE 6%, Invitrogen, cat. no. EC6265BOX) selection and purification was performed to enrich libraries containing siRNA fragments ranging from 20 to 30 nt ...
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bioRxiv - Genomics 2020Quote: ... in a QuantStudio™ 6 system (Applied Biosystems). See Supplementary Table 1 for primer sequences used in this study ...
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bioRxiv - Cancer Biology 2021Quote: ... or QuantStudio-6 Real-Time System (Applied Biosystems). Power SYBR Green Cells-to-Ct kit was used to perform quantitative PCR on 10,000 cells sorted from tumors ...
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bioRxiv - Neuroscience 2022Quote: ... in Essential 6 medium (Thermo Fisher Scientific, A1516401) supplemented with two SMAD pathway inhibitors - dorsomorphin (2.5 μM ...
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bioRxiv - Neuroscience 2022Quote: ... and 6 μl of NFW (Ambion™, AM9937). The PCR reaction was carried out using the following conditions ...
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bioRxiv - Biophysics 2022Quote: ... 6 μL PageRulerPlus Prestained Protein Ladder (ThermoFisher Scientific) was loaded to monitor the progress of the SDS-PAGE and to estimate the approximate size of the protein after staining the gel ...
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bioRxiv - Neuroscience 2021Quote: ... propanal (ACROS Organics, 1%, CAS# 123-38-6), and 1,4-diaminobutane (ACROS Organics ...
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bioRxiv - Pathology 2021Quote: ... qPCR was performed with QuantStadio 6 (Thermo Fisher). Taqman PCR probes (Life Technologies ...
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bioRxiv - Microbiology 2020Quote: ... A 6% TBE polyacrylamide gel (Novex, Thermo Scientific) was pre-run at 10 V for 10 min at 4 °C ...
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bioRxiv - Biochemistry 2020Quote: ... 6 µL of NativePAGE sample buffer (Invitrogen, BN20041) was added and 10 µL of sample was run on precast 3-12% NativePAGE gels (Invitrogen ...
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bioRxiv - Biophysics 2020Quote: ... transferred to a white 6-well plate (NUNC) and biotinylated by 30 min incubation in 4 ml ND96 solution supplemented with 0.5 mg ml−1 EZ-link sulfo-NHS-SS biotin ...
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bioRxiv - Systems Biology 2022Quote: ... ELISA assays for IL-6 (Invitrogen, catalog # KHC0061) and IL-8 (Invitrogen ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Essential 6 (E6; Thermo Fisher Scientific, A15165-01), supplemented with 1:2,000 Y-27632 (ROCK inhibitor) ...
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bioRxiv - Immunology 2020Quote: ... 6-incubation with 1 μg/mL DAPI (Invitrogen) and 2 μg/mL Wheat Germ Agglutinin (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... monocytes were cultured in 6-well plates (Nunc) in serum-free medium (5 x 106/4 ml of AIM-V ...
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bioRxiv - Microbiology 2020Quote: ... were grown in 6-well plates (Nunc™) in 2 ml 10% fetal calf serum (FCS)-enriched MEM-Eagle ...
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bioRxiv - Biochemistry 2021Quote: For MQAE (N-(Ethoxycarbonylmethyl)-6-Methoxyquinolinium Bromide) (ThermoFisher) assays U87 cells were seeded into dark 96-well microtiter flat-bottom plates at 4 × 104 cells·well-1 in 100 µL volumes DMEM media supplemented with 10% FBS and 1% Penicillin/Streptomycin and incubated overnight at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... to Essential 6 medium (Thermo Fisher Scientific, A1516401) for the first five days ...
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bioRxiv - Immunology 2021Quote: ... using a Quant Studio Flex 6 (Applied Biosystems). Samples were run in triplicate wells in a 96-well plate ...
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ORAI1 establishes resistance to SARS-CoV-2 infection by regulating tonic type I interferon signalingbioRxiv - Microbiology 2021Quote: ... or IL-6 (ThermoFisher Scientific, # 88-7066-22) using manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... suspended into GMP Essential 6 (A1516401, Life Technologies), counted with a haemocytometer and centrifuged at 300g for 5 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... in Essential 6 medium (Thermo Fisher Scientific, A1516401) supplemented with dorsomorphin (2.5 μM ...
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bioRxiv - Molecular Biology 2023Quote: ... on a QuantStudio 6 Flex instrument (Applied Biosystems) and a QuantStudio 5 Flex instrument (Applied Biosystems) ...