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Citations for Thermo Fisher :
451 - 500 of 10000+ citations for TIM 3 Human HEK 293 Fc His since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... FreeStyle 293-F cells were grown in Freestyle 293 Expression Medium (ThermoFisher Scientific). DLD1-FlpIn-TREx cells were grown in DMEM supplemented with 10% (v/v ...
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Polo-like kinase 1 independently controls microtubule-nucleating capacity and size of the centrosomebioRxiv - Cell Biology 2020Quote: ... elegans γ-tubulin complex was reconstituted by co-expression in human FreeStyle 293-F cells (Thermo Fisher Scientific). The GIP-2 and γ-tubulin (TBG-1 ...
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Targeted degradation of PCNA outperforms stoichiometric inhibition to result in programed cell deathbioRxiv - Cell Biology 2022Quote: Human embryonic kidney T-REx 293 cells which express the tetracycline repressor protein were purchased from Thermo Fisher Scientific and cultured in Eagle’s Minimum Essential Medium (MEM ...
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bioRxiv - Microbiology 2021Quote: ... Recombinant human IgG1 mAbs were produced by co-transfection of Freestyle 293-F suspension cells (Thermo Fisher Scientific) as previously described 57 and purified by affinity chromatography using protein G sepharose 4 fast flow beads (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: ... Recombinant human IgG1 mAbs were produced by co-transfection of Freestyle 293-F suspension cells (Thermo Fisher Scientific) as previously described 48 and purified by affinity chromatography using protein G sepharose 4 fast flow beads (GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: Human Embryonic Kidney Cells 293 (HEK293T cell) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Scientific™) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2024Quote: Human 293F cells were maintained at 37°C with 5-8% CO2 in FreeStyle 293 Expression Medium (ThermoFisher) supplemented with penicillin and streptomycin ...
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bioRxiv - Microbiology 2024Quote: ... Human 293F cells were maintained at 37°C with 5-8 % CO2 in FreeStyle 293 Expression Medium (ThermoFisher) supplemented with 100 I.U./mL penicillin and 100 μg/mL streptomycin.
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bioRxiv - Microbiology 2022Quote: ... pTG-Luc and SARS2-COV2 spike expression vector were transfected into HEK 293T cells at the ratio of 3:4:3 using Lipofectamine 3000 transfection reagent (ThermoFisher Scientific, Waltham, MA). Accession IDs of the spike proteins used in the study were ...
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bioRxiv - Microbiology 2021Quote: ... which is composed of two ACE2 ectodomains linked to the Fc portion of the human IgG (Anand et al., 2020).Alexa Fluor-647-conjugated goat anti-human Abs (Invitrogen) were used as secondary antibodies to detect ACE2-Fc and plasma binding in flow cytometry experiments.
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bioRxiv - Microbiology 2020Quote: ... 3 μg of mammalian expression vector plasmids based on pEF1α V5 His C (Invitrogen) encoding codon-optimized gH ...
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bioRxiv - Cancer Biology 2023Quote: ... and 10ng/ml recombinant human IL-3 (Gibco). Cultured cells were infected with lentivirus encoding GFP/Scrambled control shRNA overnight and fresh media was added the following day ...
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bioRxiv - Developmental Biology 2021Quote: ... and HEK-A (ThermoFisher) were maintained in Dulbeccos Modified Medium (DMEM ...
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Rab35 Governs Apicobasal Polarity Through Regulation of Actin Dynamics During Sprouting AngiogenesisbioRxiv - Developmental Biology 2022Quote: ... and HEK-A (ThermoFisher) were maintained in Dulbeccos Modified Medium (DMEM ...
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bioRxiv - Bioengineering 2020Quote: HEK 293F cells (Invitrogen) were cultured in Freestyle medium (Gibco ...
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bioRxiv - Cell Biology 2021Quote: ... and HEK-A (ThermoFisher) were maintained in Dulbeccos Modified Medium (DMEM ...
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bioRxiv - Biophysics 2020Quote: ... HEK 293F cells (Invitrogen) were transfected with the four plasmids and harvested after 60 hours ...
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bioRxiv - Cell Biology 2022Quote: HEK-293FT cells (Invitrogen) were cultured in 1x DMEM ...
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bioRxiv - Immunology 2022Quote: HEK-293T cells (Invitrogen) were transfected using SARS-CoV-2 S expression plasmid expression vector and lipofectamine (thermofisher ...
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bioRxiv - Immunology 2021Quote: ... or in sterile Sorting Medium [RPMI 1640 supplemented with 10% (v/v) Heat-Inactivated Fetal Bovine Serum (HI-FCS; A3840001; Gibco)] ...
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bioRxiv - Neuroscience 2024Quote: ... After blocking supernatant samples from different groups and standards (recombinant human-TREM2-His; Life Technologies) were incubated for 2 h at room temperature (RT ...
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bioRxiv - Neuroscience 2024Quote: ... PCR fragments of human αSyn were inserted into pTrc-His-TOPO vector (Thermo Fisher Scientific). His-tagged αSyn was purified by cOmplete™ His-Tag Purification Column (Roche) ...
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bioRxiv - Immunology 2022Quote: ... 3×106 HEK-293T cells were seeded in 10ml of complete DMEM Dulbecco’s Modified Eagle’s Medium (Gibco) supplemented with 10% FBS and 50μg/ml penicillin-streptomycin ...
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bioRxiv - Cancer Biology 2020Quote: ... Then the cells were stained with AlexFuor488-conjugated goat anti human Fc (Life technologies) at RT for 30 min and analyzed by flow cytometry ...
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bioRxiv - Immunology 2021Quote: ... Horseradish peroxidase (HRP)-conjugated antibody specific for the Fc region of human IgG (Invitrogen) was used as secondary antibody to detect antibody binding in ELISA experiments.
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bioRxiv - Microbiology 2021Quote: ... 100 μL of HRP-labelled anti-human IgG Fc antibody (Catalog# 31413, Thermo Fisher) was added to each well and incubated for 1 hour ...
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bioRxiv - Bioengineering 2021Quote: ... nanovials were modified with biotinylated goat anti-human IgG Fc antibody (Thermo Fisher Scientific). Samples were resuspended in DMEM and seeded in a well plate ...
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bioRxiv - Microbiology 2022Quote: ... Horseradish peroxidase (HRP)-conjugated Abs specific for the Fc region of human IgG (Invitrogen) were used as secondary antibodies to detect antibody binding in ELISA experiments ...
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bioRxiv - Immunology 2020Quote: ... Rabbit anti-Human IgG Fc HRP Secondary Antibody (ThermoFisher Scientific Catalog #31423, 1:10,000), and Rabbit anti-Human IgG F(ab’)2 HRP Secondary Antibody (ThermoFisher Scientific Catalog #31482 ...
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bioRxiv - Biochemistry 2021Quote: ... and 0.25 µL Goat anti-Human IgG Fc PE conjugate (ThermoFisher #12-4998-82). Protease susceptibility measurements were performed in biological replicates (n = 2 ...
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bioRxiv - Biochemistry 2021Quote: ... and 0.25 µL Goat anti-Human IgG Fc PE conjugate (ThermoFisher #12-4998-82). Titrations were performed in biological replicates (cultures from independent colonies grown on separate days ...
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bioRxiv - Immunology 2020Quote: ... followed by addition to the Fc-chimeric human ACE2-coated MaxiSORP ELISA plate (Nunc) for an hour at room temperature ...
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bioRxiv - Cell Biology 2023Quote: Human JAGGED1-Fc (Martin et al., 2023) was transfected into Expi293F cells (ThermoFisher, A14527) using FectroPro (Polyplus ...
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bioRxiv - Microbiology 2021Quote: ... HEK-293T overexpressing the human ACE2 were kindly provided by Integral Molecular Company and maintained in DMEM (Invitrogen) with 10% fetal bovine serum ...
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bioRxiv - Microbiology 2020Quote: ... Human embryonic kidney (HEK) 293T cells (ATCC CRL-3216) were cultured in DMEM+GlutaMAX-I (Thermo Fisher Scientific) supplemented with heat-inactivated 8% FBS.
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bioRxiv - Microbiology 2021Quote: ... HEK-293T overexpressing the human ACE2 were kindly provided by Integral Molecular Company and maintained in DMEM (Invitrogen) with 10% fetal bovine serum ...
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bioRxiv - Microbiology 2022Quote: ... human embryonic kidney (HEK) 293T and Vero cells (ATCC) were maintained with Dulbecco’s Modified Eagle’s medium (DMEM) (Gibco), supplemented with 10% fetal calf sera (FCS ...
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bioRxiv - Microbiology 2023Quote: ... Human embryonic kidney 293T cells (HEK 293T) were maintained in DMEM GlutaMAX (Dulbecco’s Modified Eagle Medium, ThermoFisher Scientific) supplemented with decomplemented FCS 10% (ThermoFisher Scientific ...
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bioRxiv - Genetics 2024Quote: Human embryonic kidney (HEK)-293T cells (ATCC CRL-3216) were cultured in 10% FBS DMEM (Gibco 11965-092), supplemented with penicillin (50 U per mL ...
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bioRxiv - Molecular Biology 2022Quote: Female human embryonic kidney cells HEK-293FT and male human fibrosarcoma HT1080 cells were cultured in DMEM-GlutaMAX + Pyruvate (Life Technologies SAS, Saint-Aubin, France), supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Cell Biology 2021Quote: ... FreeStyle™ 293-F cells were grown in FreeStyle™ 293 Expression Medium (Gibco) under constant agitation at 37 °C with 5% CO2 in a humidified incubator.
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bioRxiv - Biochemistry 2021Quote: ... FreeStyle 293-F cells were grown in suspension in FreeStyle 293 Expression Medium (Gibco).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cDNA plasmids in pcDNA™4/TO Mammalian Expression Vector were transiently transfected into Expi293F™ cells stably expressing human FHF2b and human SCN1B subunit (polyclonal) background using ExpiFectamine™ 293 Transfection Kits (Gibco,Thermo Fisher Scientific CAT #: A14524). Induction was achieved using Tetracycline (Sigma Aldrich) ...
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bioRxiv - Microbiology 2024Quote: ... in 0.5% (w/v) skim milk/PBS-T of HRP-conjugated anti-human IgG Fc or anti-mouse IgG Fc (Invitrogen™, Waltham, MA, USA) antibodies for membranes incubated with 2G12 or anti-p24 mAbs ...
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bioRxiv - Molecular Biology 2020Quote: ... in 1.5ml of SD/-Leu/-His liquid medium (plus 3 mM 3-AT) were incubated in a 12-well microplate (Thermo Scientific) at 30°C for 96 hours (h) ...
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bioRxiv - Bioengineering 2023Quote: ... Protein expression was achieved by transient transfection of FreeStyle human embryonic kidney 293 (HEK293-F) cells (Thermo Fisher Scientific). Cells were grown in non-baffled shake flasks (Corning ...
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A quantitative analysis of the interplay of environment, neighborhood and cell state in 3D spheroidsbioRxiv - Systems Biology 2020Quote: T-REx-293 cells (Invitrogen) and DLD1 cells (Flp-In T-Rex DLD-1 ...
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bioRxiv - Microbiology 2021Quote: ... T-REx-293 cells (Invitrogen) were grown in DMEM supplemented with blasticidin (10 µg/mL ...
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bioRxiv - Microbiology 2021Quote: T-REx-293 cells (Invitrogen), which constitutively expresses the Tet repressor (TetR ...
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bioRxiv - Microbiology 2021Quote: FreeStyle 293-F (Thermo Fisher) cells were grown to a density of 1×106 cells/mL at 37°C with 8% CO2 with regular 135 rpm agitation ...