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Citations for Thermo Fisher :
651 - 700 of 711 citations for Diphtheria Toxin CRM197 Mutant since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: Wild-type and mutant (C139A) USP39 with a Flag-tag at its N-terminal were cloned into the pLenti6/V5 lentiviral vector (Invitrogen, California, USA). Specific shRNA oligonucleotides targeting USP39 were cloned into the pLL3.7 lentiviral vector (Addgene) ...
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bioRxiv - Genetics 2023Quote: ... 60-90% confluency) were transfected with 2 µg of plasmids carrying the indicated ROMK mutants using Lipofectamine 2000 (Invitrogen, Waltham, MA, USA), and the media was replaced after 4 hr ...
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bioRxiv - Microbiology 2023Quote: Expi293F cells were transfected with 500 ng of pCEP4-myc-EFNB2 (wildtype or mutants) per mL of culture at 2 ξ 106 cells/mL using Expifectamine (ThermoFisher Scientific). At 24 h post-transfection ...
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bioRxiv - Microbiology 2023Quote: Expi293F cells were transfected with 500 ng of pCEP4-myc-EFNB2 (wildtype or mutants) per mL of culture at 2 ξ 106 cells/mL using Expifectamine (ThermoFisher Scientific). At 24 h post-transfection ...
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bioRxiv - Molecular Biology 2023Quote: ... Wild type TGM2 and the TGM2-C277A mutant were overexpressed in HEK 293T cells using Lipofectamine 2000 Transfection Reagent (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2024Quote: Protein extracts from wt and CG1603 mutant larvae tissues (48h after egg laying) were prepared using the RIPA buffer (MilliporeSigma) with Halt Protease Inhibitor Cocktail (Thermo Fisher Scientific), 5 mM NaF (MilliporeSigma ...
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bioRxiv - Immunology 2024Quote: ... BMMCs were transfected with Regnase-1 or −3 (WT or RNase-inactive mutant D141N or D252N) IVT mRNA using the 10 µl Neon Transfection System Kit (Thermo Fisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Plant Biology 2024Quote: ... The ripE1 fragment in pDONR207 was used as template to generate ripE1 phosphosite mutants by site-directed mutagenesis using the QuickChange Lightning Site-Directed Mutagenesis Kit (Life technologies, USA) following the manufacturer’s instructions and the primers indicated in Table S2 ...
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bioRxiv - Biochemistry 2023Quote: ... and WT or mutant hTR (pBSU1-hTR) were transfected at a 1:3 molar ratio using lipofectamine 2000 (11668019, Thermo Fisher Scientific). The cells were further expanded 3-fold 24 h after transfection and then 24 h later either harvested or used in DMS probing reactions ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: The activity of CYP3A5 promoted by WT or mutant POR was tested using the fluorogenic substrate BOMCC (Invitrogen Corp, Carlsbad, CA, United States). The purified CYP3A5 (CYPEX ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: The activity of CYP2C9 promoted by WT or mutant POR was tested using the fluorogenic substrate BOMCC (Invitrogen Corp, Carlsbad, CA, United States). The purified CYP2C9 (CYPEX ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: The activity of CYP2C19 promoted by WT or mutant POR was tested using the fluorogenic substrate EOMCC (Invitrogen Corp, Carlsbad, CA, United States). The purified CYP2C19 (CYPEX ...
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bioRxiv - Biochemistry 2019Quote: The thermal stability (Tm) for the mutants were determined using the Protein Thermal shift (PTS)™ kit made by Applied BioSystem ® from Thermo Fisher. Following the standard protocol by the manufacturer ...
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bioRxiv - Immunology 2019Quote: ... the wild-type and mutant (C302/305R) recombinant Mul1 proteins were produced in Sf9 cells using the Bac-to-Bac Baculovirus expression system (Invitrogen, Carlsbad, CA, USA) and purified by a nickel-chelating resin (Life Technologies ...
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bioRxiv - Plant Biology 2020Quote: ... Three wild-type or five rdr2 mutant embryos were pooled before DNA extraction with the GeneJET Plant Genomic DNA Purification Kit (Thermo Fisher Scientific, K0791). Embryos from rdr2/RDR2 heterozygous mothers were individually collected ...
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bioRxiv - Biophysics 2020Quote: All pET28a-N proteins (WT and mutants) and nsp12 protein were labeled by incubating with a 1:1 molar ratio of Alexa Fluor™ 546 carbox (Thermo Fisher Scientific) for 1 h at room temperature with continuous stirring ...
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bioRxiv - Molecular Biology 2020Quote: ... The copy ratio of mutant to wild type allele in each sample was determined using QuantStudio™ 3D AnalysisSuite™ software (Applied Biosystems). The DNA from the sequenced samples was tested along with DNA from uninfected wheat leaf ...
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bioRxiv - Molecular Biology 2020Quote: ... Resulting Bgt cytb wild type (G143 allele; strobilurin-sensitive) and mutant (A143 allele; strobilurin-resistant) primers and probe combinations were ordered as a pre-mix (Thermo Fisher Scientific, Australia). The probe targeting A143 allele was labelled with the fluorophore FAM on its 5’ end and the probe targeting the G143 allele labelled with VIC ...
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bioRxiv - Microbiology 2021Quote: Two μg of wild-type or mutant S was transfected into Vero or A549 cells using the Lipofectamine 3000 system (Invitrogen; ratios described above). Eighteen to twenty-four hours post transfection ...
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bioRxiv - Cancer Biology 2021Quote: ... PC-3 cells were mock-transfected or transfected with 0.5 to 4 μg LDHA-WT or LDHA-mutant using Lipofectamine 3000 (Invitrogen, Thermo Fisher Scientific, Bremen, Germany). For the migration assay ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: The activity of CYP2C9 promoted by WT or mutant POR was tested using the fluorogenic substrate BOMCC (Invitrogen Corp, Carlsbad, CA, United States). The purified CYP2C9 (CYPEX ...
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Mechanotransduction-dependent control of stereocilia dimensions and row identity in inner hair cellsbioRxiv - Cell Biology 2019Quote: Inner ears were isolated from C57BL/6J mice or mutant mice littermates at the indicated ages and dissected in cold Hank’s balanced salt solution (Cat#14025076, Thermo Fisher Scientific, Waltham, MA) supplemented with 5 mM HEPES ...
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bioRxiv - Biochemistry 2020Quote: CHO-K1 cells in 10-cm dishes were transfected with complementary DNA encoding FLAG-tagged mLPCAT2 or one of several FLAG-tagged mLPCAT2 mutants using Lipofectamine 2000 (Thermo Fisher Scientific, USA). At 48 h post-transfection ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: The activity of CYP2C19 promoted by WT or mutant POR was tested using the fluorogenic substrate EOMCC (Invitrogen Corp, Carlsbad, CA, United States). The purified CYP2C19 (CYPEX ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: The activity of CYP3A5 promoted by WT or mutant POR was tested using the fluorogenic substrate BOMCC (Invitrogen Corp, Carlsbad, CA, United States). The purified CYP3A5 (CYPEX ...
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bioRxiv - Immunology 2020Quote: Wild-type Phl p 6 and its mutants were expressed from pET17b constructs in Escherichia coli strain BL21 Star (DE3; Invitrogen/Thermo Fisher Scientific), as described in the supplement.
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bioRxiv - Biochemistry 2021Quote: ... Synthetic DNA encoding the genomic sequence for both WT and mutant versions of each gene were purchased as ‘Strings DNA Fragments’ from GeneArt (Thermo Fisher Scientfic, UK). These were cloned into the vector pAW8-natMX6 (Watson et al ...
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bioRxiv - Biochemistry 2021Quote: The ATPase activity of WT SidJ and its mutants was measured in a UV-transparent microplate using the EnzChek Pyrophosphate Assay Kit (ThermoFisher Scientific, E-6645). The assay was performed in triplicates ...
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bioRxiv - Microbiology 2021Quote: Two μg of wt or mutant S protein was transfected into Vero or A549 cells using the Lipofectamine 3000 system (Invitrogen; ratios described above). Eighteen to twenty-four hours post transfection ...
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bioRxiv - Immunology 2021Quote: The SARS-CoV-2 receptor-binding domain (RBDwt) and the RBD mutant (RBDmut) (K417N, E484K, N501Y) were expressed using Expi293F cells (Invitrogen, ThermoFisher Scientific, MA, USA). The genes that encode SARS-CoV-2 RBDwt (residues Arg319-Phe541 ...
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bioRxiv - Molecular Biology 2020Quote: ... 3) and four tyr (clutch no. 7) mutants using the Invitrogen TOPO TA kit according to the manufactures protocol (Invitrogen catalogue no. K4575J10), and Sanger sequenced the extracted plasmids from 6-10 colonies per embryo (Fig ...
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bioRxiv - Immunology 2021Quote: The SARS-CoV-2 receptor-binding domain (RBDwt) and the RBD mutant (RBD417, RBD501, RBD484 and RBDtrip) were expressed using Expi293F cells (Invitrogen, ThermoFisher Scientific, MA, USA). The genes that encode SARS-CoV-2 RBDwt (residues Arg319-Phe541 ...
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bioRxiv - Immunology 2022Quote: ... Synchronized L4 stage WT and pals-25(Q293*)icb98 mutant animals were collected and RNA was extracted by standard procedure using TRIzol (Life Technologies, Carlsbad, CA). RNA samples were sequenced by Genewiz (Azenta Life Sciences ...
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bioRxiv - Neuroscience 2022Quote: Stable HEK293 cells exhibiting tetracycline-inducible expression of wild type (WT) and mutant (R92C) GLT8D1 were generated using the Flp-In™ T-REx™ system (ThermoFisher Scientific). The pGKFLPobpA vector (Addgene ...
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bioRxiv - Molecular Biology 2022Quote: ... Wild-type HEK293T and two independent clonal lines of CRISPR Serf2 KO mutant HEK 293T cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Gibco; high glucose, pyruvate, 41966052), supplemented with 10 % Bovine Cow Serum (BCS ...
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bioRxiv - Molecular Biology 2023Quote: 2×105 HeLa cells stably expressing wild-type hSSB1-GFP and 102A/115A/102&115A mutants were seeded onto CELLview™ Culture dish (35mm) (Greiner, 627860. After 16h, 10 µM Hoescht 33342 (Thermo Scientific, H3570) was used to sensitize cells for 10 min before laser damage ...
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bioRxiv - Molecular Biology 2023Quote: Recombinant pPICZαA plasmids containing the WT and mutant THCAS were used to transform the Komagataella phaffii strain X-33 (Invitrogen™, Thermo Fisher Scientific), as specified by the supplier ...
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bioRxiv - Molecular Biology 2023Quote: Recombinant pPICZαA plasmids containing the WT and mutant THCAS were used to transform the Komagataella phaffii strain X-33 (Invitrogen™, Thermo Fisher Scientific), as specified by the supplier ...
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bioRxiv - Biochemistry 2023Quote: Kinetic measurements of TrpBwt and its mutants were performed by monitoring 5-fluoro-L-Trp formation in a plate reader (Thermo Scientific Varioskan Lux) over 20 min at 290 nm using ΔE290 = 1.89 mM−1·cm−1 ...
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bioRxiv - Neuroscience 2023Quote: ... cDNAs encoding for CpxII or its mutant variants were sub-cloned into the first open reading frame (ORF) of a bicistronic Semliki Forest vector (pSFV1, Invitrogen, San Diego, CA). Enhanced-GFP expression from the second ORF allowed for the identification of infected cells.
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bioRxiv - Molecular Biology 2023Quote: Plasmids for transient expression of Gal4-tagged UNK mutants (Figures 1 and 2) were created using the Gateway cloning strategy where UNK mutants in the pENTR/D-TOPO vector backbone (Thermo Fisher Scientific, K240020) were transferred in an LR reaction using the Gateway LR Clonase II Enzyme mix (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... L380A mutants) of lamin A/C and were inserted into the pProEx-HTa vector for overproduction of the lamin fragments (Thermo Fisher Scientific, MA, USA). The used oligonucleotide primer sequences are listed in Table EV1 ...
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bioRxiv - Cell Biology 2022Quote: ... and the S1PR1 mutant 3’UTR plasmid (100 ng) with miR155 (50 nM) were co-transfected into HEK293T cells by lipofectamine 2000 (Thermo Fisher Scientific Inc. USA). After 48 h ...
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bioRxiv - Biophysics 2022Quote: ... was spiked with 1% of Alexa 488-labeled HU-B mutant (containing an N-terminal Cys conjugated with Alexa Fluor™ 488 C5 Maleimide from Thermofisher; Cat no. A10254). The 488 nm laser was set at 100% for efficient bleaching and focused on a small circular region with a diameter of 1 μm ...
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bioRxiv - Microbiology 2021Quote: ... mutants were generated by transforming the generated parC mutants with the respective gyrA non-WT alleles and selecting on TSYA plates supplemented with 12 mg/L ciprofloxacin (Acros Organics, New Jersey, USA). GyrA and parC allelic replacements in the transformants were confirmed by PCR and Sanger sequencing.
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bioRxiv - Immunology 2021Quote: The SARS-CoV-2 receptor-binding domain (RBDwt) and the RBD mutant (RBD417, RBD501, RBD484 and RBDtrip) were expressed using Expi293F cells (Invitrogen, ThermoFisher Scientific, MA, USA). The genes that encode SARS-CoV-2 RBDwt (residues Arg319-Phe541 ...
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bioRxiv - Immunology 2021Quote: The SARS-CoV-2 receptor-binding domain (RBDwt) and the RBD mutant (RBDmut) (K417N, E484K, N501Y) were expressed using Expi293F cells (Invitrogen, ThermoFisher Scientific, MA, USA). The genes that encode SARS-CoV-2 RBDwt (residues Arg319-Phe541 ...
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bioRxiv - Biophysics 2023Quote: ... TDP-43 (wild-type and A315T/Q331K mutants), and circularly permutated Venus were joined (T43V, T43V173, and T43V195) and subcloned into pcDNA3.1(+) (Thermo Fisher Scientific, Waltham, MA, USA) (pcDNA-T43V ...
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bioRxiv - Plant Biology 2024Quote: ... Plasmids encoding fluorescently tagged mutant CMV 2b proteins were generated using Gateway® cloning following the protocol provided (Invitrogen, Thermo-Fisher Scientific, Paisley UK) with attB-flanked DNA fragments cloned into the pDONR221 donor plasmid ...
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bioRxiv - Plant Biology 2024Quote: ... Plasmids encoding fluorescently tagged mutant CMV 2b proteins were generated using Gateway® cloning following the protocol provided (Invitrogen, Thermo-Fisher Scientific, Paisley UK) with attB-flanked DNA fragments cloned into the pDONR221 donor plasmid ...