Labshake search
Citations for Thermo Fisher :
201 - 250 of 711 citations for Diphtheria Toxin CRM197 Mutant since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2020Quote: ... The mutant constructs were prepared by PCR mutagenesis using the Phusion polymerase (ThermoFisher Scientific). The TOP5 and FOP5 luciferase constructs were described previously (28) ...
-
bioRxiv - Molecular Biology 2020Quote: ... the RNase E mutant was grown in 25 µg/ml kanamycin (Kan) (Fisher Scientific), the SgrS A177U ...
-
bioRxiv - Cell Biology 2020Quote: ... and the (AP)6 and GS Linker SNAP25b mutants were synthesized by Thermo Fisher Scientific (Loughborough ...
-
bioRxiv - Biochemistry 2020Quote: ... The wild-type or mutant 5-HT2C was cloned into the pcDNA3.1 vector (Invitrogen) for transfection ...
-
bioRxiv - Cancer Biology 2021Quote: ... mutant or empty vector (0.5 μg) was combined with 2 μL Lipofectamine 2000 (ThermoFisher) in OptiMEM medium (Gibco ...
-
bioRxiv - Microbiology 2021Quote: ... Wild type and mutant plasmids were transformed into MAX Efficiency Stbl2 Competent Cells (Invitrogen) for maxi-DNA preparations ...
-
bioRxiv - Immunology 2022Quote: ... was used to generate Flag-tagged Prom1 clones and deletion mutants in pcDNA3.1+ (Invitrogen). The primers used for cloning are listed in Supplementary Table 1.
-
bioRxiv - Neuroscience 2022Quote: ... AAV.DUX4 mutant constructs.V5 or pCINeo (transfection control plasmid) using Lipofectamine 2000 reagent (Thermo Scientific). Cells were harvested 24 hours post-transfection in RIPA buffer containing 10 mM Tris-Cl (pH 8.0) ...
-
bioRxiv - Cell Biology 2022Quote: Total RNA was extracted from control or dwg8 mutants using TRIzol® reagent (Invitrogen). We synthesized the first strand cDNA with 1 µg of total RNA using iScriptTM Reverse Transcription Supermix (BIO-RAD ...
-
bioRxiv - Biochemistry 2023Quote: ... and AtSWEET1 mutants were cloned into pDRf1-GW or p112A1NE using Gateway cloning (Invitrogen) for yeast expression ...
-
bioRxiv - Biochemistry 2023Quote: ... C35S (CxxS) and the double mutant C32S/C35S (SxxS) was cloned into pDONR221 (Invitrogen) by Gateway recombination ...
-
bioRxiv - Microbiology 2023Quote: ... The mutant sequences of each frame were amplified with Phusion DNA polymerase (Thermo Scientific) by a pair of primers that bound to the flanking sequences on the oligonucleotides ...
-
bioRxiv - Microbiology 2023Quote: ... fumigatus maiA mutant strains were performed using Hygromycin B (Thermo Fisher, Waltham, MA, USA) as a selection marker ...
-
bioRxiv - Biochemistry 2023Quote: ... ClpL NTD mutants A-E were generated utilizing synthetic DNA as template (Invitrogen™) and restriction cloning ...
-
bioRxiv - Genetics 2023Quote: Each sample (wild type and mutant variants) was amplified using SimpliAmp thermocycler (Applied Biosystems). For each sample ...
-
bioRxiv - Cancer Biology 2024Quote: BRAF mutant melanoma cell lines were grown in DMEM/F12 (Gibco Ref 11330-032) supplemented with 10% fetal bovine serum and 1% pen/strep ...
-
bioRxiv - Developmental Biology 2024Quote: ... Wildtype and mutant PCR fragments were TOPO cloned into pCR8/GW/TOPO (Invitrogen, K250020) and sequenced (Psomagen) ...
-
bioRxiv - Neuroscience 2019Quote: The retrograde tracers were cholera toxin b subunit (CTb) conjugated to Alexa Fluor 488 (CTb-488) (Cat#C34775 ThermoFisher Scientific) or Alexa Fluor 555 (CTb-555 ...
-
bioRxiv - Neuroscience 2021Quote: ... pairwise injections of 80 nL of 0.5% (wt/vol) fluorescently labeled cholera toxin B subunit (CTB-488, ThermoFisher C22841, CTB-647, ThermoFisher C34788). In C57BL6/J mice ...
-
bioRxiv - Biophysics 2020Quote: ... The lipid ordered phase was visualized through GM1 crosslinking by incubating the PMS in 10 µg/ml of Alexa-488 labeled cholera-toxin subunit B (Invitrogen) for 2 h at 37°C and IFITM3-iSNAP was stained with 3 µM SNAP-cell 647-SIR (New England Biolabs ...
-
bioRxiv - Neuroscience 2020Quote: ... Cholera Toxin subunit-B (Luppi et al., 1995) with a Alexa Fluor-647 (AF-647) conjugate (ThermoFisher, catalog number C34778) was prepared by dissolving 100μg in 20μL PBS ...
-
bioRxiv - Neuroscience 2020Quote: The retrograde tracer cholera toxin subunit B (CTB) conjugated with Alexa Fluor™ 555 (CTB-555) was purchased from Invitrogen, Thermo Fisher Scientific (Waltham ...
-
bioRxiv - Microbiology 2020Quote: ... Mouse monoclonal antibodies against binary toxin subunits CDTa and CDTb and anti-Clostridia GDH monoclonal mouse antibody (Clone: J44D, Invitrogen) were obtained from Merck ...
-
bioRxiv - Biophysics 2020Quote: ... DiIC18(1,1’-Dioctadecyl-3,3,3’,3’-Tetramethylindotri-carbocyanine Iodide) and the Alexa 488-labeled fragment B of cholera toxin were purchased from Thermo Fisher scientific (Waltham ...
-
bioRxiv - Neuroscience 2022Quote: Projection neurons were retrograde labeled from their axon terminals by pressure injection of Alexa-conjugated Cholera Toxin B (Ctb, Invitrogen) or Fast Blue (Polysciences ...
-
bioRxiv - Neuroscience 2022Quote: ... SCPN (CBN + CSN) or CSN only were retrogradely labeled by bilateral injection of Cholera Toxin B subunit (CTB; Thermo Scientific) into either cerebral peduncles (at 3 injection sites ...
-
bioRxiv - Microbiology 2020Quote: ... GM1 coated microtiter plates were incubated with a 1:20 dilution of culture supernatant and detected using primary anti-cholera toxin and secondary HRP-conjugated goat anti-rabbit IgG (Invitrogen). 1-Step Ultra TMB-ELISA (Thermo Scientific ...
-
bioRxiv - Neuroscience 2021Quote: ... nwS1 was stereotaxically injected with 80-100 nl cholera toxin subunit B (CTB) conjugated to Alexa Fluor 488 (CTB-488, Invitrogen), and wS1 with CTB-Alexa Fluor 555 (CTB- 555 ...
-
Cbln1 directs axon targeting by corticospinal neurons specifically toward thoraco-lumbar spinal cordbioRxiv - Neuroscience 2022Quote: CSN that project to lumbar spinal segments were retrogradely labeled at P5 with an Alexa Fluor 555-conjugated cholera toxin subunit B (CTB-555) recombinant retrograde tracer (Invitrogen). For these injections ...
-
bioRxiv - Neuroscience 2023Quote: P12 rats were deeply anesthetized with isoflurane and the sclera and cornea were pierced with a syringe containing a 1% solution of cholera toxin B subunit (Invitrogen) conjugated to either Alexa Fluor 488 (green ...
-
bioRxiv - Neuroscience 2023Quote: ... Drd1afl/fl mice received bilateral injections of Alexa Fluor 594-conjugated Cholera Toxin Subunit B in PBS (CTB-594, 1.0 mg mL−1, no. C-34776, Thermo Fisher) in either LH or VTA brain regions.
-
bioRxiv - Pathology 2023Quote: Vip3Aa toxin was biotinylated with a 1:30 (protein:biotin) molar ratio of EZ-Link Sulfo- NHS-LC-Biotin (Life Technologies) for 30 minutes on ice ...
-
bioRxiv - Bioengineering 2023Quote: ... the bacterial toxin ccdB was cloned with the enzymes KpnI and NheI using a dedicated commercial ccdB-resistant strain (ThermoFisher); the generated plasmid LentiCRISPRV1-gRNA-TetR-intron-ccdB was used to obtain the KpnI/NheI digested backbone for lentiviral library ...
-
bioRxiv - Neuroscience 2023Quote: ... regenerating axons were anterogradely labeled by intravitreal injection of 2 µl Alexa Fluor 555-conjugated cholera toxin β subunit (CTB, 0.5% in PBS; Invitrogen).
-
bioRxiv - Genomics 2023Quote: ... 0.5 mg/mL hydrocortisone (SigmaAldrich, #H0888), 100 ng/mL Cholera toxin (SigmaAldrich, #C8052, and 10 µg/mL insulin (ThermoFisher #12585014). To generate MCF10A cells stably expressing PiggyBac-NLS-ZapCV2 ...
-
bioRxiv - Biophysics 2021Quote: ... Fn39-10 S1417C mutant was fluorescently labeled with Alexa Fluor 488 C5 maleimide (ThermoFisher Scientific) at residue Cys-1417 ...
-
bioRxiv - Developmental Biology 2019Quote: Lung tissues from Fstl1 mutant and control mice were collected and homogenized in Trizol (Ambion). RNA extraction and reverse transcription was performed following standard procedures (RevertAid First Strand cDNA Synthesis Kit ...
-
bioRxiv - Microbiology 2021Quote: ... crescentus ParB (WT/mutants) were amplified by PCR and Gibson assembled into plasmid pENTR (Invitrogen) so that parB is flanked by phage attachment sites attL1 and attL2 i.e ...
-
bioRxiv - Neuroscience 2020Quote: ... or its mutants in the pGEMSH vector using the mMessage mMachine kit (Thermo Fisher Scientific). cRNA (40 ng ...
-
bioRxiv - Plant Biology 2021Quote: ... IF1 mutant constructs were generated using the GeneArt Site-Directed Mutagenesis System (Thermo Fisher Scientific) and AccuPrime Pfx DNA Polymerase (Thermo Fisher Scientific) ...
-
bioRxiv - Plant Biology 2020Quote: ... All wild-type and mutant cording sequences were cloned into pCR8/GW/D-TOPO (Invitrogen) as level 0 modules (Weber et al. ...
-
bioRxiv - Cell Biology 2020Quote: Cells were transiently transfected with the indicated mutants using Lipofectamine 2000 (11668019; Thermo Fisher Scientific). After 24 hours ...
-
bioRxiv - Neuroscience 2022Quote: ... We then cloned the truncated mutants into a gateway entry vector pDONR221 (Invitrogen Cat# 12536017). We verified the sequence by sequencing and subcloned it into a yeast gateway vector to allow GAL-inducible expression of the ORF with a C-terminal EGFP or mRuby3 tag (pAG416GAL-ccdB-EGFP in Lindquist Advanced Gateway Vector Collection ...
-
bioRxiv - Cancer Biology 2020Quote: ... WT and mutant T-47D cells were cultured in RPMI 1640 media (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2019Quote: ... the above mutants are sub-cloned into pAc5.1/V5-His-A (Thermo Fisher Scientific, V411020) with EcoRI and XhoI (New England Biolabs).
-
bioRxiv - Molecular Biology 2021Quote: ... Recombinant baculoviruses expressing A2AAR WT or mutants were prepared using Bac-to-Bac system (Invitrogen). Spodoptera frugiperda 9 (Sf9 ...
-
bioRxiv - Biophysics 2020Quote: ... Mutant cRNA were made with the mMessage T7 polymerase kit (Applied Biosystems-Thermo Fisher Scientific).
-
bioRxiv - Biophysics 2020Quote: ... Mutant cRNA were made with the mMessage T7 polymerase kit (Applied Biosystems-Thermo Fisher Scientific).
-
bioRxiv - Genomics 2019Quote: ... RNA was isolated in triplicate for each mutant strain by the TRIzol reagent protocol (Invitrogen) followed by miRNeasy mini column (Qiagen) ...
-
bioRxiv - Immunology 2021Quote: ... SIVmac239 gp120 and other Env mutant constructs were transiently expressed in FreeStyle 293F cells (ThermoFisher) as described previously (von Bredow et al. ...