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Citations for Thermo Fisher :
551 - 600 of 711 citations for Diphtheria Toxin CRM197 Mutant since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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TrkB induced metastatic potential of cancer by suppression of BMP mediated tumor inhibitory activitybioRxiv - Cancer Biology 2020Quote: ... to generate TrkB overexpression cells [24] and and the TrkB K588M mutant using previously described [42] was generated by site-directed mutagenesis with Site-Directed Mutagenesis Kit (ThermoFisher Scientific). To generate a stable knockdown of TrkB ...
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bioRxiv - Cell Biology 2021Quote: ... GFP or GFP-tagged USP7 WT and mutants were then subcloned into the pcDNA5/FRT/TO vector (Invitrogen, Thermo Fisher Scientific) and sequence verified before generating inducible FlpIn T-Rex cell lines ...
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bioRxiv - Cell Biology 2021Quote: ... GFP or GFP-tagged USP7 WT and mutants were then subcloned into the pcDNA5/FRT/TO vector (Invitrogen, Thermo Fisher Scientific) and sequence verified before generating inducible FlpIn T-Rex cell lines ...
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bioRxiv - Bioengineering 2019Quote: ... were tested before deletion mutant promoters were synthesized by modifying the RCV promoter 25 or GRK1 promoter 26 (Figure S1c, Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... total cellular RNA was isolated from MLL and SETD1A mutant cells or parent U-2OS cells using the TRIzol (Ambion, Inc.,) or using an RNA isolation kit (Zymo Research) ...
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bioRxiv - Biochemistry 2022Quote: ... and co-transfected with wild type and mutant constructs of PKD 20 h post-seeding with 250 ng of wild-type and 250 ng mutant plasmid DNA per well using TurboFect (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: Total RNA was extracted from ∼2 mg of right atrium (RA) tissues of 1-year-old Dnajb6+/- heterozygous mutant hearts and WT sibling controls using Trizol reagent (ThermoFisher Scientific) following the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2022Quote: ... 1.0X106 of patient cells carrying the MED13L mutant allele were suspended in 80 μl of Opti-MEM medium (Invitrogen, Carlsbad, CA). The cell suspension was combined with 20 μl of genome editing constructs containing either 6 μg of Cas12a dRT and 6 μg of the matching sgRNA/Cas12a expression plasmid or 6 μg of Cas9 dRT and 6 μg of the matching sgRNA/Cas9 expression plasmid ...
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bioRxiv - Biophysics 2022Quote: ... and SN25 FL (1–206, R59C) or truncation mutant (11–206, R59C) were labeled with 5×molar excess Tetramethylrhodamine-5-maleimide (TMR) (Molecular Probes) in 25 mM HEPES pH 7.4 ...
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bioRxiv - Microbiology 2021Quote: Point mutations to generate the mutants were introduced by site-directed mutagenesis of phCMV-SARS-CoV-2-Spike using Q5 polymerase (Thermo Scientific) and validated by sanger sequencing ...
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bioRxiv - Cell Biology 2022Quote: ... and pT2A-CMH-FLAG-NUP98-HOXA9 or its mutants (Kawakami and Noda, 2004; Urasaki et al., 2006) using Lipofectamine 2000 (Life Technologies). After 2 days ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells containing WT or mutant HPV16 were grown in the same medium also containing 150 μg/mL G418 (Thermo Fisher Scientific). All cells were routinely checked for mycoplasma contamination.
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bioRxiv - Cell Biology 2019Quote: ... THD-EGFP wild-type or mutant sequences downstream of the Tet-On responsive element were cloned into the pcDNA5/FRT plasmid (Life Technologies) expressing the Tet-On transactivator from the mouse PGK promoter ...
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bioRxiv - Cell Biology 2019Quote: ... RNA was isolated from whole eyes of 6-month old cep290 mutant and sibling control animals (n = 9 per group) with Trizol (ThermoFisher 15596026). Reverse transcription using 1 μg RNA was performed with the iScript cDNA Synthesis kit (Bio-Rad 1708891) ...
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bioRxiv - Synthetic Biology 2020Quote: ... and 0.06 μg of CMV-promoter driven plasmid encoding SARS-CoV-2 wild type or mutant spike variants using Lipofectamine™ 2000 transfection reagent (ThermoFisher). Pseudovirus was harvested by collecting supernatant 48 h after transfection and filter sterilized (0.44 μm ...
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bioRxiv - Genetics 2020Quote: We PCR amplified and cloned the IL7 wildtype (rs201412253-Val18) and mutant (rs201412253-18Ile) open reading frame (ORF) in the pcDNA5/FRT vector (ThermoFisher Scientific) using HindIII and BamHI restriction sites (see Supplementary Table 22 for ORF and primer sequences) ...
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bioRxiv - Biophysics 2020Quote: The tau cysteine mutants were buffer exchanged into 20 mM NaPi buffer (pH 8) via 7k MWCO Zeba spin desalting columns (Thermo Fisher). 200 μL of 50 μM protein aliquots were reacted with 50 molar equivalents of methyl 2,5-dibromopentanoate (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: COS-7 cells were seeded on 6-well plates and transfected the next day with 1 μg of WT-eNOS and 1 μg of Hsp90 mutant constructs or pcDNA using Lipofectamine 2000 (Life Technologies) in Opti-MEM I Reduced Serum Medium (Life Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... along with 50 ng and 100 ng of expression plasmids encoding 3xHA-tagged wildtype or mutant mouse PORCN-D as indicated using Lipofectamine 2000 (Thermo Fisher). 24 hours after transfection ...
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bioRxiv - Neuroscience 2019Quote: Full-length Eya1 wild type and mutant D328A protein in a GST-fusion format were generated using the Bac-to-Bac Baculovirus Expression system (ThermoFisher Scientific) and expressed in Sf9 insect cells ...
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bioRxiv - Developmental Biology 2019Quote: Ten to twenty manually dechorionated 24 hpf embryos of a germline mutant incross and a germline wildtype incross were homogenized in TRIzol (Ambion, 15596018). Subsequently ...
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bioRxiv - Biophysics 2020Quote: ATPase activity of prohead/gp16 (wt or mutant) motor complexes was determined by measuring production of inorganic phosphate by the EnzChek Phosphate Assay Kit (Life Technologies) as described previously (43) ...
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bioRxiv - Genomics 2020Quote: Total RNA was extracted from frozen mammary tissue of wild type and mutant mice using a homogenizer and the PureLink RNA Mini kit according to the manufacturer’s instructions (Thermo Fisher Scientific). Total RNA (1 μg ...
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bioRxiv - Cancer Biology 2021Quote: ... or the catalytically dead mutant H1304Y, D1306A (pcDNA3-Flag-TET2 mCD, addgene #72220, 18) were grown in high glucose and pyruvate containing DMEM (Gibco 41966) supplemented with 10% fetal calf serum (Eurobio S116365181H) ...
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bioRxiv - Genomics 2020Quote: ... Cre/LoxP site-specific integration of a Rarg-DamR126A fusion construct (subcloned from a previously published Tcf7l2-DamN126A mutant construct35 was performed followed by G418 (Life Technologies) selection (350 μg/mL ...
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bioRxiv - Immunology 2020Quote: ... HEK 293T cells were transfected with CARD8 WT and mutant UPA-CARD-mCherry constructs (0.02 μg) using lipofectamine 2000 (Thermo Fisher Scientific). 48 hours after transfection ...
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bioRxiv - Microbiology 2020Quote: ... Fluorescent signals of eGFP production in wild type and mutant strains were measured by flow cytometry (Attune NxT Flow Cytometer, Thermo Fisher) (53).
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bioRxiv - Genetics 2019Quote: ... and mutant detection was performed on adult legs with the Phire Animal Tissue Direct PCR kit (Thermo Fisher Scientific, Waltham, MA) with added LCGreen Plus+ Melting dye (Idaho Technology Inc. ...
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bioRxiv - Microbiology 2022Quote: ... cells were treated with compound at a final DMSO concentration of 2% prior to transfection with 100 ng of the wild-type or triple mutant (L50F E166A L167F) plasmid with Lipofectamine LTX (ThermoFisher#15338100) for 24h ...
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bioRxiv - Cell Biology 2022Quote: The trimeric S-protein and its mutants were produced by transient transfection of Expi293F high-density cells with the ExpiFectamine 293 (Thermo Fisher) lipid cationic transfection reagent according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... FLAG-tagged human DNA2 nuclease-deficient (D277A) and helicase-deficient (K654R) mutants were generated by Phusion site-directed mutagenesis (Thermo Fisher) of wild-type DNA2-FLAG (pIF494 ...
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bioRxiv - Biochemistry 2022Quote: Purified PPARγ AF-1 D33C+Q121C mutant protein at 50 µM was labeled with Alexa Fluor 488 and Alexa Fluor 647 maleimide dyes (Thermo Fisher) in 20 mM sodium phosphate pH 7.2 ...
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bioRxiv - Molecular Biology 2022Quote: ... HEK 293T cells were transiently transfected with 5 µg of FLAG tagged full-length HRI construct and 5 µg of GFP tagged DELE1 constructs (DELE1CTD or mutants) using Lipofectamine 3000 transfection reagent (L3000008, ThermoFisher Scientific). GFP empty vector was used as a negative control ...
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bioRxiv - Microbiology 2022Quote: BHK-21 cells were transfected with 1 μg of pCAGGS plasmid encoding the NL09 wild-type or escape mutant HA proteins using Lipofectamine (Invitrogen, USA) and Plus reagent (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: BHK-21 cells in 96-well plate were transfected with 100 ng of pCAGGS NL09 HA (wild-type or escape mutants) using Lipofectamine 3000 (Invitrogen, USA). At 24 h post-transfection ...
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bioRxiv - Plant Biology 2022Quote: ... The basal 0.5-2.5 cm of leaf 4 from wprb1/+;wprb2/+ and wprb1/b1;wprb2/b2 mutants were fixed and stained with Alexa fluor 488-phalloidin (Thermo Fisher). Nuclei and cell walls were stained using 10 μg mL-1 propidium iodide (Thermo Fisher) ...
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bioRxiv - Microbiology 2023Quote: ... ModB R73A and rS1 mutants were generated by site-directed mutagenesis using a procedure based on the Phusion Site-Directed Mutagenesis Kit (Thermo Scientific). The resulting plasmids were sequenced and transformed into E ...
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bioRxiv - Biochemistry 2022Quote: ... Cac1 mutants from the pDONR/Zeo plasmids were then sub-cloned into the pDEST8 vector via Gateway technology (Thermo Fisher Scientific) followed by Sanger sequencing verification (Supplementary Table 1) ...
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bioRxiv - Genomics 2023Quote: Total RNA was extracted from frozen mammary tissue of wild type and mutant mice using a homogenizer and the PureLink RNA Mini kit according to the manufacturer’s instructions (Thermo Fisher Scientific). Total RNA (1 μg ...
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bioRxiv - Molecular Biology 2023Quote: ... The stable wild-type hSSB1-GFP and 102A/115A/102&115A hSSB1-GFP mutants were generated with Lipofectamine 3000 (Invitrogen, L3000001) transfection followed by 500 μg/ml hygromycin B (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... and two PALS1 deletion mutants (ΔECR: aa 118-674, ΔL27N: aa 178-675) using the pENTRTM Directional TOPO® Cloning Kit (Invitrogen). The pENTR constructs were shuttled into modified pQCXIH-GW-GFP or pQCXIH- GW-Halo plasmids using the L/R reaction of the GatewayTM Cloning system (Invitrogen) ...
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bioRxiv - Biophysics 2023Quote: 12-well cultures of Hep-2 cells at 70% confluence were transfected with 0.5 μg of wild-type or mutant UL34 or UL31-FLAG plasmid using Lipofectamine as described by the manufacturer (Gibco-BRL) and incubated at 37 °C overnight ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The transfection reagent was mixed with the pOG44 Flp-Recombinase expression vector and the pCDNA5/TO/FRT-3xMyc-eGFP-CLIP-170 WT or one of the mutant vectors with a 3:1 ratio in Opti-MEMTM I medium (Gibco, ThermoFisher Scientific). Selection started 48h after the transfection in a Dulbecco’s modified Eagle medium (DMEM ...
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bioRxiv - Biochemistry 2023Quote: ... HEK293 cells were transfected with PNKP-FLAG (WT and mutants) expressing vector (pcDNA3) in 6-well plates using Lipofectamine 2000 (ThermoFisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Relative expression levels for zebrafish vtg2 in F2 vtg2- mutant and wild type female liver were measured using SYBR GREEN qPCR Master Mix (SYBR Green Master Mix kit; Applied Biosystems) as indicated by the manufacturer in a total volume of 10 µl ...
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bioRxiv - Microbiology 2023Quote: ... and 3 µg of a plasmid expressing the WT or mutant C-prM-E structural genes of interest using Lipofectamine 3000 (Cat# L3000-015; ThermoFisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... cells were transfected with 1.5 ug of WT or mutant SARS-CoV-1 S plasmid using polyethylenimine (PEI) (Thermo Fisher Scientific). Cells were transfected for 24 hours ...
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bioRxiv - Cancer Biology 2023Quote: B-ALL cell lines (NALM6 and RS4;11) and NALM6 phospho-GR mutants (GR-S203A and GR-S226A) were tested with combinations of prednisolone (Acros Organics, #449470250) and the PI3Kδ inhibitor idelalisib (Gilead) ...
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bioRxiv - Cell Biology 2022Quote: Inner ears from C57BL/6J mice or mutant mice littermates dissected at the indicated ages in cold Hank’s balanced salt solution (Cat# 14025076, Thermo Fisher Scientific) supplemented with 5 mM HEPES ...
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bioRxiv - Cell Biology 2022Quote: ... Inner ears from C57BL/6J mice or mutant mice littermates dissected at the indicated ages in cold Hank’s balanced salt solution (Cat# 14025076, Thermo Fisher Scientific) supplemented with 5 mM HEPES ...