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Citations for Thermo Fisher :
551 - 600 of 756 citations for Diphtheria Toxin CRM197 Mutant since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2023Quote: ... or mutant PADI2 (with L642A or T159 or W161A or L642A/W161A, or L642A/W161A/T159A) using Lipofectamine 3000 (Invitrogen) in 96-well plates ...
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bioRxiv - Biochemistry 2023Quote: ... and p300BRPHZT 4A- and 4E-substituted mutant proteins were expressed in baculovirus-infected High Five insect cells (Thermo Fisher Scientific). Baculoviruses were produced using the Bac-to-Bac baculovirus expression system (Invitrogen) ...
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bioRxiv - Cancer Biology 2023Quote: Cells were transfected with PB2 full-length and mutant overexpression plasmids via a Lipofectamine 3000 Transfection Kit (Thermo Fisher #L3000015). Cells were plated at 300k cells/well in a 6-well plate the day prior to transfection ...
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bioRxiv - Biophysics 2023Quote: ... were transiently co-transfected together with MKK6DD mutants and p38α pcDNA3.1 plasmids (or empty vector for controls) with Lipofectamin2000 (Thermo Scientific) (Fig ...
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bioRxiv - Cell Biology 2023Quote: ... PCR products of ame1 and okp1 point mutants (with C-terminal TAF tag and G418 marker) were amplified from the mutant plasmids using a high-fidelity DNA polymerase (SuperFi, Invitrogen) and transformed into the respective haploid ame1 and okp1 null mutants ...
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bioRxiv - Molecular Biology 2023Quote: ... Sequences encoding KNL12026-2342 and ZWINT wild-type and mutants were cloned into a pCDNA5/FRT/TO-EGFP-IRES plasmid and co-transfected with pOG44 (Invitrogen), encoding the Flp recombinase ...
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bioRxiv - Systems Biology 2023Quote: ... NSP16 and NSP10 mutant cDNA entry clones were generated by gene synthesis and subcloning into pDONR221 (GeneArt, Thermo Fisher Scientific). All cDNA clones were sequence verified and shuttled into LuTHy destination plasmids ...
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bioRxiv - Cell Biology 2023Quote: Day 6 moDCs were transfected with GFP-WT cofilin or mCherry-labelled mutant cofilin using a Neon electroporator (Thermofisher Scientific). moDCs were pulsed twice at 1000 V for 40 ms and using 10 µg of the corresponding plasmid ...
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bioRxiv - Plant Biology 2023Quote: ... The WT and mutant NICA PCR products were recombined into the intermediate vector pDonor207 using the BP recombination kit (Invitrogen), and then recombined into the destination vector pEarleygate104 using the LR recombination kit (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... The point mutants of CNNMs were obtained by mutagenesis using the QuikChange Lightning Site-Directed Mutagenesis Kit (Thermo Fisher Scientific). The amino acid sequence of the CBS-pair domain of murine CNNM2 (495-582 ...
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bioRxiv - Biophysics 2023Quote: ... 26 The αSA140C and αSA18C mutants were conjugated with AlexaFluor 647 and AlexaFluor 568 NHS-esters (Thermo Fisher Scientific, USA) targeting the cysteine residues ...
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bioRxiv - Genomics 2022Quote: ... and old (50 days) wDah control and RpII215C4 mutant animals were dissected in replicates of 5 and collected in DMEM (#41965-047, Gibco) without supplements ...
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bioRxiv - Plant Biology 2023Quote: ... genomic DNA from each mutant plant was isolated by using the Phire Tissue Direct PCR Master Mix (Thermo Fisher Scientific), followed by amplifying the sgRNA-targeted regions and subsequent Sanger sequencing of the obtained PCR fragments ...
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bioRxiv - Plant Biology 2023Quote: ... the wild-type and mutant versions were inserted in the pIX-HALO destination vector using the Gateway LR Clonase II Enzyme Mix (Invitrogen). Positive clones were screened by colony PCR and confirmed by Sanger sequencing ...
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bioRxiv - Microbiology 2023Quote: ... We transfected HEK293T cells with pCAGGS alphavirus E3–E2–(6K/TF)– E1 wild-type or mutant expressor plasmids using Lipofectamine 3000 (Invitrogen) or an empty pCAGGS vector ...
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bioRxiv - Evolutionary Biology 2023Quote: ... were transfected with either wild-type PADI2 (WT) or the PADI2 triple mutant (with L642A/W161A/T159A) using Lipofectamine 3000 (Invitrogen) in 60mm plates ...
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bioRxiv - Cell Biology 2023Quote: ... mRNAs encoding Xenopus SART1 full-length and deletion mutants were prepared from the pCS2+ vectors using mMESSAGE mMachine SP6 kit (Invitrogen). The mRNAs were added to the depleted extract at the beginning of the cell-cycle reactions.
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bioRxiv - Plant Biology 2024Quote: ... Clones for sub-cellular localization and mutant complementation were generated using Gateway® Technology as per the manufacturer’s instructions (Invitrogen). The sequences of interest were amplified from cDNA employing primers with attB1 and attB2 sites that flank gene-specific nucleotides ...
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bioRxiv - Biophysics 2024Quote: ... Then cRNA of WT and mutant KCNQ1 were synthesized using the mMessage T7 polymerase kit (Applied Biosystems-Thermo Fisher Scientific) for oocyte injections.
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bioRxiv - Plant Biology 2024Quote: An inducible MDF overexpressor transgenic line was designed for comparative studies with the loss-of-function mdf-1 mutant and constructed using Gateway cloning (Invitrogen). All the primers were designed by SnapGene software and sequences are available in Suppl ...
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bioRxiv - Biophysics 2024Quote: ... Then cRNA of WT and mutant KCNQ1 were synthesized using the mMessage T7 polymerase kit (Applied Biosystems-Thermo Fisher Scientific) for oocyte injections.
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bioRxiv - Bioengineering 2024Quote: ... Bands were detected with the SuperSignal West Pico Plus or SuperSignal West Femto (for the P41 mutants) substrates (Thermo Fisher) and a ChemiDoc XRS+ imaging system (Bio-Rad).
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bioRxiv - Microbiology 2024Quote: Mutant was verified by extracting DNA with the KingFisher using the KingFisher™ Ready DNA Ultra 2.0 Prefilled Plates (ThermoFisher). The library was prepared with the NEBNext Ultra II FS DNA Library Prep Kit for Illumina ...
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TAK1 operates at the primary cilium in non-canonical TGFB/BMP signaling to control heart developmentbioRxiv - Developmental Biology 2024Quote: ... The P19CL6 wildtype and Tak1 and Tab2 mutants were cultured in T25 flask at 37°C and 5% CO2 in MEM alpha medium (Gibco) supplemented with 10% FBS (Gibco ...
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bioRxiv - Microbiology 2024Quote: ... Mutant colonies were picked and AvrB presence was checked by PCR using Promega GoTaq™ DNA polymerase (Fisher Scientific, USA) and specific primers (FOR ...
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bioRxiv - Biochemistry 2024Quote: ... followed by an enzyme solution consisting of 8 nM wild-type PETase or its mutant labeled with 0.8 nM Qdot655 (Thermo Scientific), 2 mM 2-Mercaptoethanol (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... 100,000 cGAS knockout HeLa cells were transiently co-transfected with the cGAMP reporter (or cGAMP-blind mutant) with or without 10 µg cGAMP using the Neon transfection system (Invitrogen, 10 µl tips ...
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bioRxiv - Cell Biology 2024Quote: ... Rescue cell lines with stable expression of N-terminally-tagged Flag- or Strep-TTC5 (WT and mutants) were generated from the TTC5 knockout cells using the Flp-In system (K650001 Invitrogen) according to manufacturer’s protocol ...
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bioRxiv - Biophysics 2020Quote: The engineered Cys residue in β or PCNA mutants was covalently modified with tetramethylrhodamine-5-maleimide or Alexa Fluor 546 C5 maleimide (ThermoFisher) and purified from the free fluorophore as described previously (9,10) ...
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bioRxiv - Developmental Biology 2020Quote: HEK293 cells expressing wild-type or mutant ATF6 were lysed in SDS lysis buffer (2% SDS in PBS containing protease and phosphatase inhibitors (Thermo Scientific). Protein concentrations of the total cell lysates were determined by BCA protein assay (Pierce) ...
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Interaction of the Xanthomonas effectors XopQ and XopX results in induction of rice immune responsesbioRxiv - Molecular Biology 2020Quote: The wild-type copy of xopX and its 14-3-3 protein binding motif mutants were cloned by Gateway cloning (Invitrogen, California) from the pENTR clones to the BiFC vector pDEST-VYCE(R)GW carrying the C-terminal region of the Venus Fluorescent Protein (VFP ...
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bioRxiv - Cell Biology 2019Quote: ... Entry clones of myosin XI (WT and mutants) and 3mEGFP were cloned into a moss expression plasmid with LR clonase (Thermo Fisher), yielding pB1-MyoHNC-B5-gtail-mutation-B5-3mEGFP-B2 ...
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bioRxiv - Cell Biology 2019Quote: DNA fragments coding for MKLP1620-858 (wild type and AQA mutant) were generated by PCR and cloned into pDEST15 (Thermo Fisher) to express N-terminal GST tagged polypeptides in E ...
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bioRxiv - Cell Biology 2019Quote: ... 10 μg of purified GST-MKLP1620-858 proteins (wild type and AQA mutant) bound to beads were phosphorylated in vitro with 2 μg of recombinant His-tagged Aurora B (Thermo Fisher) as described10,27 and then washed three times with 500 μl of phosphatase buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... coli cells expressing WT GST-vWA and GST-vWA-C152S mutant proteins were resuspended in PBS supplemented with 2 mM TCEP (Thermo scientific), 0.01 mg/mL lysozyme from chicken egg (SIGMA) ...
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TrkB induced metastatic potential of cancer by suppression of BMP mediated tumor inhibitory activitybioRxiv - Cancer Biology 2020Quote: ... to generate TrkB overexpression cells [24] and and the TrkB K588M mutant using previously described [42] was generated by site-directed mutagenesis with Site-Directed Mutagenesis Kit (ThermoFisher Scientific). To generate a stable knockdown of TrkB ...
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bioRxiv - Cell Biology 2021Quote: ... GFP or GFP-tagged USP7 WT and mutants were then subcloned into the pcDNA5/FRT/TO vector (Invitrogen, Thermo Fisher Scientific) and sequence verified before generating inducible FlpIn T-Rex cell lines ...
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bioRxiv - Cell Biology 2021Quote: ... GFP or GFP-tagged USP7 WT and mutants were then subcloned into the pcDNA5/FRT/TO vector (Invitrogen, Thermo Fisher Scientific) and sequence verified before generating inducible FlpIn T-Rex cell lines ...
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bioRxiv - Bioengineering 2019Quote: ... were tested before deletion mutant promoters were synthesized by modifying the RCV promoter 25 or GRK1 promoter 26 (Figure S1c, Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... total cellular RNA was isolated from MLL and SETD1A mutant cells or parent U-2OS cells using the TRIzol (Ambion, Inc.,) or using an RNA isolation kit (Zymo Research) ...
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bioRxiv - Biochemistry 2022Quote: ... and co-transfected with wild type and mutant constructs of PKD 20 h post-seeding with 250 ng of wild-type and 250 ng mutant plasmid DNA per well using TurboFect (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: Total RNA was extracted from ∼2 mg of right atrium (RA) tissues of 1-year-old Dnajb6+/- heterozygous mutant hearts and WT sibling controls using Trizol reagent (ThermoFisher Scientific) following the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2022Quote: ... 1.0X106 of patient cells carrying the MED13L mutant allele were suspended in 80 μl of Opti-MEM medium (Invitrogen, Carlsbad, CA). The cell suspension was combined with 20 μl of genome editing constructs containing either 6 μg of Cas12a dRT and 6 μg of the matching sgRNA/Cas12a expression plasmid or 6 μg of Cas9 dRT and 6 μg of the matching sgRNA/Cas9 expression plasmid ...
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bioRxiv - Biophysics 2022Quote: ... and SN25 FL (1–206, R59C) or truncation mutant (11–206, R59C) were labeled with 5×molar excess Tetramethylrhodamine-5-maleimide (TMR) (Molecular Probes) in 25 mM HEPES pH 7.4 ...
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bioRxiv - Microbiology 2021Quote: Point mutations to generate the mutants were introduced by site-directed mutagenesis of phCMV-SARS-CoV-2-Spike using Q5 polymerase (Thermo Scientific) and validated by sanger sequencing ...
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bioRxiv - Cell Biology 2022Quote: ... and pT2A-CMH-FLAG-NUP98-HOXA9 or its mutants (Kawakami and Noda, 2004; Urasaki et al., 2006) using Lipofectamine 2000 (Life Technologies). After 2 days ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells containing WT or mutant HPV16 were grown in the same medium also containing 150 μg/mL G418 (Thermo Fisher Scientific). All cells were routinely checked for mycoplasma contamination.
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bioRxiv - Cell Biology 2019Quote: ... THD-EGFP wild-type or mutant sequences downstream of the Tet-On responsive element were cloned into the pcDNA5/FRT plasmid (Life Technologies) expressing the Tet-On transactivator from the mouse PGK promoter ...
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bioRxiv - Cell Biology 2019Quote: ... RNA was isolated from whole eyes of 6-month old cep290 mutant and sibling control animals (n = 9 per group) with Trizol (ThermoFisher 15596026). Reverse transcription using 1 μg RNA was performed with the iScript cDNA Synthesis kit (Bio-Rad 1708891) ...
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bioRxiv - Synthetic Biology 2020Quote: ... and 0.06 μg of CMV-promoter driven plasmid encoding SARS-CoV-2 wild type or mutant spike variants using Lipofectamine™ 2000 transfection reagent (ThermoFisher). Pseudovirus was harvested by collecting supernatant 48 h after transfection and filter sterilized (0.44 μm ...