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Citations for Thermo Fisher :
451 - 500 of 711 citations for Diphtheria Toxin CRM197 Mutant since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... the cells with either ATF6 plasmids or ATF6-mutant plasmids were transfected with 200 ng of FGF2-ERSE-luciferase-reporter plasmids using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2021Quote: Total RNA was isolated from adult DRG from 6-month old mutant and wild-type mice (4 per genotype, mixed male and female groups) using the RNaqueous Micro kit (Ambion). All DRGs were pooled for each sample to obtain enough RNA for analysis ...
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Interaction of the Xanthomonas effectors XopQ and XopX results in induction of rice immune responsesbioRxiv - Molecular Biology 2020Quote: The wild-type copy of the xopX gene and its 14-3-3 protein binding motif mutants were cloned in the yeast two-hybrid vector pDEST32 (Invitrogen) using the Gateway cloning system (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... or 1 μg of Super8-FOPflash (8 mutant TCF-binding sites) reporter plasmids (kindly provided by Randall Moon, University of Washington, Seattle) using Lipofectamine 2000 (Invitrogen); a thymidine kinase-Renilla plasmid (0.1 μg ...
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bioRxiv - Developmental Biology 2022Quote: RNA was isolated from heart ventricles of G2-Gata4+/+;Itga4flox/+ control and G2-Gata4Cre/+;Itga4flox/flox mutant E11.5 embryos using the Arcturus Picopure RNA isolation kit (Applied Biosystems), including a step for removing genomic DNA ...
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bioRxiv - Cell Biology 2019Quote: ... HeLa cell lines with single integrated copies of KIF4A wild type and the mutants described in the figures were created using the T-Rex doxycycline-inducible Flp-In system (Invitrogen). HeLa cell lines with GFP or mScarlet inserted in frame at the C-terminus of KIF4A and SMC2 in the endogenous loci were constructed using CRISPR/Cas9 (Alfonso-Perez et al. ...
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bioRxiv - Biochemistry 2019Quote: ... the polynucleotides encoding GtACR1 mutants were fused in frame with a C-terminal eight-His tag and subcloned into the pPIC9K vector (Invitrogen) between EcoRI and NotI sites ...
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bioRxiv - Neuroscience 2019Quote: A pool of twenty 5dpf WT and rnaset2sh532 mutant zebrafish larvae were incubated at 27°C for 20 minutes in 5µg/ml of Acridine Orange (Life Technologies) diluted in zebrafish E3 medium ...
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bioRxiv - Microbiology 2019Quote: ... were infected subcutaneously (sc) in each footpad with 103 pfu of nLuc-expressing EEEV mutant viruses in 10 ul of OptiMEM media (Invitrogen). For WEEV ...
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bioRxiv - Microbiology 2021Quote: The parB (WT/mutant) genes were recombined into a Gateway-compatible destination vector pMT571-1xFLAG-DEST via LR recombination reaction (Invitrogen). For LR recombination reactions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and 0.2 μg empty pQCXIP or pQCXIP-WT IFITM3 or the indicated mutant fused with amino-terminal FLAG using Lipofectamine2000 (Invitrogen). At 48 hours post-transfection ...
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bioRxiv - Neuroscience 2020Quote: ... The pCAG-DCC:TDTOMATO wildtype and missense mutant receptor constructs (0.2 µg) were transfected into COS-7 cells using Lipofectamine® 2000 (Invitrogen). After 48 hours ...
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bioRxiv - Neuroscience 2020Quote: The pCAG-DCC:TDTOMATO wildtype and missense mutant receptor constructs (0.2 µg) were transfected into COS-7 cells using Lipofectamine® 2000 (Invitrogen). After 48 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were co-transfected with either a constant concentration of 0.1 μg of NF1-NanoLuc pcDNA expression plasmid or CRAF RBD-NanoLuc pcDNA expression plasmid with 0.2 μg of GFP-tagged KRAS (WT or Mutant) with 0.25 μl of Lipofectamine 2000 per well following the manufacturer’s protocol (Thermo Fisher Scientific). Twenty-four hours later ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were transiently transfected with mCherry-tagged wild type or mutant ORP1L at 70% confluency using Lipofectamine 2000 reagent (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: Atg18WT and mutant DNA were amplified from the corresponding pRS316 plasmids and cloned into a pEXP5-NT/TOPO vector (Invitrogen). Plasmids were transformed into E ...
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bioRxiv - Genetics 2021Quote: ... The hGOT1-GFP and mutant hGOT1-GFP constructs were transfected into Chinese Hamster Ovary (CHO) (ExpiCHO-S) cells with an ExpiFectamine Transfection Kit (Gibco) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: RBDwt of SARS-CoV-2 and mutant RBDs (RBDK417N, RBDE484K, RBDN501Y, RBDK417N/E484K/N501Y, and RBDL452R/E484Q) were expressed using Expi293F cells (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and mutant GLP-1R constructs containing designed signal alanine mutation were integrated into CHOFlpIn cells using FlpIn Gateway technology system (Invitrogen). Stable CHOFlpIn expression cell lines were selected using 600 μg/ml hygromyocin B ...
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bioRxiv - Cell Biology 2021Quote: ... 100 μL reactions containing 10 μL FLAG-xCENP-C mutants in interphase CENP-C-depleted extract were added to 10 μL Protein A Dynabeads (Invitrogen) coupled to 2.5 μg mouse anti-FLAG antibody ...
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bioRxiv - Cell Biology 2021Quote: ... 100 μL reactions containing 10 μL FLAG-xHJURP truncations or mutants in CSF-arrested or interphase extract were added to 10 μL Protein A Dynabeads (Invitrogen) coupled to 2.5 μg mouse anti-FLAG antibody ...
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bioRxiv - Cell Biology 2020Quote: pENTR containing myc-6xHis Sec24C or Sec24D WT or mutants were used in a Gateway LR Clonase II Enzyme reaction (11791020, Invitrogen) according to manufacturer’s instructions with pLenti CMV/TO Puro DEST (670-1 ...
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bioRxiv - Cell Biology 2022Quote: Cells were transfected with the plasmid encoding the DN-KASH or the Rap1Q63E (Rap1*) mutant using Lipofectamin 2000 (Life Technologies).
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bioRxiv - Biochemistry 2022Quote: ... After 3hrs from release of the nocodazole block cells were double transfected with pEGFP-human LA (EGFP-wt LA and mutants) and RFP-HP1α using Lipofectamine 2000 (Invitrogen) in accordance with the manufacturer’s protocol at early passages having 60-70% confluency and were kept in culture for 24 hrs.
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bioRxiv - Plant Biology 2022Quote: ... and mCherry/pDONRP2rP3 into pK7m34GW (phospho-mutants) or pH7m34GW (truncations and DPF deletion) expression vectors (Karimi et al., 2002) by LR Clonase II (Invitrogen).
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bioRxiv - Plant Biology 2022Quote: ... Resulting mutant variants of CAP1 were cloned in versions with and without stop codons into pDONR221 Gateway entry vectors (Invitrogen) by BP Clonase ...
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bioRxiv - Biochemistry 2022Quote: Chimeric SHh precursor encoding HA-tagged NLuc fused to native and mutant forms of human Sonic HhC autoprocessing domain were assembled in pDisplay (Invitrogen). Fragments encoding Sonic HhC were inserted into pDisplay using PstI and XhoI ...
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bioRxiv - Cell Biology 2022Quote: ... Cells at ∼20% confluency were then transfected with 0.5 µg of the Sun1-myc and FLAG-KASH5 (FL, ΔTM, or mutant FL) constructs using Lipofectamine LTX and Plus reagents (Invitrogen) in Opti-MEM (Gibco) ...
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bioRxiv - Neuroscience 2020Quote: RNA extraction from 30 zebrafish larvae from the same genotype (homozygous mutant or wild-type) was performed using the TRIzol reagent (Invitrogen) according to the recommended protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 x 105 ESCs were co-transfected with 1.5 μg wild-type or mutants of PB-ATRX-HA-Neo and 1.5 μg Super PiggyBac transposase using Lipofectamine 3000 (Thermo Fisher). After two days of transfection ...
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bioRxiv - Biophysics 2020Quote: Wild-type and the three LXXAA mutants of TIF2NRID were amine-labelled with a fluorescent probe (Alexa 488 NHS Ester, ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... RELN or different RELN mutants (Y1821H, G1280E, I650S, D556V, R913C, C539R, R3207C) was performed for 4 hrs using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... 293T cells were transfected with 2.0 μg pBAC-BToV or -HE mutant and - EGFP with 1.5 μL Lipofectamine 3000 and 1.0 μL P3000 Regent (Thermo Fisher Scientific) and incubated at 37°C ...
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bioRxiv - Biochemistry 2021Quote: The wild-type (WT) and mutant GLP-1R constructs were integrated into FlpIn-CHO cells using FlpIn Gateway technology system (Invitrogen). Stable CHOFlpIn expression cell lines were selected using 600 μg/ml hygromyocin B ...
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bioRxiv - Microbiology 2021Quote: ... MBCS mutant and S686G S were titrated by preparing 10-fold serial dilutions in Opti-MEM I (1X) + GlutaMAX (Gibco). Thirty μl of each dilution was added to monolayers of 2 × 104 VeroE6 ...
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bioRxiv - Neuroscience 2021Quote: ... we dissected wild-type or acj6 mutant brains expressing HRP-rCD2 by PNs in pre-chilled Schneider’s medium (Thermo Fisher), removed the optic lobes ...
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bioRxiv - Biophysics 2020Quote: ... the Wylie mutants were first sub-cloned into a pBAD202 expression vector available in the pBAD directional TOPO expression kit (Invitrogen). The native leader peptide sequence of the β-lactamase gene was retained to achieve periplasmic transport for proper folding of the enzyme ...
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bioRxiv - Systems Biology 2022Quote: ... an absence of Syn1 mRNA expression in ΔSyn1 mutants was confirmed by qPCR using High Capacity Reverse Transcriptase (Applied Biosystems) and Fast SYBR Green reagents in a StepOne Plus Real Time PCR System (Applied Biosystems ...
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bioRxiv - Cell Biology 2019Quote: Recombinant baculovirus carrying full-length USP7/wt or deletion mutants of USP7 were generated with the Bac-to-Bac System (Invitrogen). Infected Sf9 cells were grown in spinner culture for 48–96 h at 27°C and His-tagged protein-purified using Ni2+-NTA agarose (Invitrogen ...
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bioRxiv - Biophysics 2019Quote: ... Then cRNA of WT KCNQ1 and mutants were synthesized using the mMessage T7 polymerase kit (Applied Biosystems-Thermo Fisher Scientific) for oocyte injections.
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bioRxiv - Biophysics 2019Quote: ... Then cRNA of WT KCNQ1 and mutants were synthesized using the mMessage T7 polymerase kit (Applied Biosystems-Thermo Fisher Scientific) for oocyte injections.
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bioRxiv - Cell Biology 2019Quote: ... fap81 double mutant were imaged by cryo-ET using a Titan Krios transmission electron microscope (Thermo Fisher Scientific, Hillsboro, OR) equipped with a K2 direct electron detection camera (Gatan ...
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bioRxiv - Genetics 2021Quote: Total RNA was isolated from the eggs at 24 hpo from a wild-type strain (p50T) and three b-4 mutant strains (e36, e37, and No. 903) using TRIzol (Invitrogen). Reverse transcription was then performed using the oligo (dT ...
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bioRxiv - Physiology 2020Quote: ... stable inducible cell lines expressing WT or mutant EAAT2 were generated by selection of Flp-In T-Rex 293 cells (Invitrogen) transfected with pcDNA5/FRT/TO-EAAT2 and used either with or without induction with tetracycline (38,50,52).
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bioRxiv - Molecular Biology 2021Quote: The wild-type and mutant SARS-CoV-2 S glycoproteins were expressed transiently by a pcDNA3.1(-) vector (Thermo Fisher Scientific) (29) ...
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bioRxiv - Microbiology 2021Quote: ... cells transfected with the SARS-CoV-2 S protein deletion mutants and controls were washed once with DPBS (Fisher Scientific), fixed with 4% (w/v ...
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bioRxiv - Developmental Biology 2021Quote: ... Select rhoaa coding sequences were PCR-amplified from wild type or rhoaa-mutant (R17, Δ3) zebrafish cDNAs and cloned into pCRII-TOPO (Invitrogen), according to manufacturer’s specifications ...
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bioRxiv - Immunology 2021Quote: ... Cells grown to a density of 3 million cells per mL were transfected using pCMV::SARS-CoV-2_S_NTD derivative mutants with the ExpiFectamine™ 293 Transfection Kit (ThermoFisher Scientific) with and cultivated for five days at which point the supernatant was harvested ...
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bioRxiv - Developmental Biology 2019Quote: ... 3 control or 3 Prdm16 mutant neural stem cell cultures were grown for two days before RNA extraction by use of TRIzol reagent (Invitrogen). 4 ug of total RNA was further cleaned with Turbo DNase (Ambion ...
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bioRxiv - Biochemistry 2020Quote: ... T-rex 293 cells (approximately 3 × 106 cells per 10-cm dish) were transfected with 5-HT2C wild-type or mutant DNA using Lipofectamine 2000 (Invitrogen) following manufacturer’s protocols ...