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Citations for Thermo Fisher :
351 - 400 of 711 citations for Diphtheria Toxin CRM197 Mutant since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... 20 WT and 20 s741 mutant larvae were incubated with 6.4 μM BODIPY FL C5 (Invitrogen, Waltham, MA) that was suspended in an egg yolk feed at room temperature with agitation for 7 hours ...
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bioRxiv - Immunology 2022Quote: ... or its truncation mutant were single- or co-transfected into HEK 293T cells by Lipofectamine LTX (ThermoFisher, 15338100) for 16 h ...
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bioRxiv - Biophysics 2020Quote: Capped cRNAs encoding WT and mutant GLIC were transcribed in vitro using the mMessage mMachine T7 kit (Ambion). Oocytes were obtained from an in-house X ...
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bioRxiv - Neuroscience 2019Quote: ... with the wildtype and mutant constructs was performed using lipofectamine 2000 according to the manufacturer’s recommendations (ThermoFisher Scientific). Successful transfectants were selected using 100 µg/ml hygromycin B (ThermoFisher Scientific ...
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bioRxiv - Synthetic Biology 2019Quote: ... The soluble recombinant MDH mutants were purified by using HisPur™ Ni-NTA Spin columns (ThermoFisher Scientific, USA). The columns with loaded supernatants were washed with Wash buffer (0.1 M Potassium phosphate buffer ...
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bioRxiv - Synthetic Biology 2020Quote: ... Each mutant was individually transformed to BL21(DE3) cells for downstream protein expression and purification steps (Thermo Fisher). Tagged nucleotides were synthesized as described previously28 ...
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bioRxiv - Cancer Biology 2019Quote: ... the wild-type and mutant 3’UTRs were cloned into a luciferase reporter vector (pmiR-REPORT, Ambion, USA) at the SpeI and HindIII site to generate Luc-HIF1α-WT and Luc-HIF1α-Mut constructs ...
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bioRxiv - Developmental Biology 2019Quote: Cortices of control and Prdm16 KO mutant E13.5 embryos were dissected for RNA extraction using Trizol reagent (Invitrogen). RNA quality of three biological replicates was tested by Agilent Bioanalyzer ...
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bioRxiv - Developmental Biology 2019Quote: ... day 4- and day 6-control and mutant ESC-derived neurospheres were collected and treated with Trizol (Invitrogen). RNAs were quantified by a Nanodrop instrument ...
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bioRxiv - Physiology 2021Quote: ... M7kd or its mutants encoded in pIRES2-EGFP vectors (2.5 µg/dish) were transfected using lipofectamine 3000 (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... The DNA template for the mutant and the wild type MLKL154 were sub-cloned into pEGFPN2 (Life Technologies) vector using XhoI and BamHI (NEB ...
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bioRxiv - Cell Biology 2020Quote: ... Both WT SOD2 and SOD2 mutant plasmids were transfected into ZF4 cells using Lipofectamine 3000 Transfection Reagent (Invitrogen).
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bioRxiv - Molecular Biology 2022Quote: ... and the ABE 7.10 mutant region was PCR amplified with Phusion High-Fidelity PCR Master Mix (Thermo Fisher) for 20 cycles and sequenced by Genewiz amplicon-EZ sequencing ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... cells stably expressing human M4 mAChR or mutant constructs were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Invitrogen) containing 5% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2022Quote: ... Wildtype and mutant coding sequences were transferred to the pEF1/V5-His vector (cat. no. V920; Life Technologies) for overexpression ...
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bioRxiv - Biochemistry 2022Quote: ... baculoviruses harboring yCAF-1 subunits and mutants were made using the Gateway technology subcloning system (Thermo Fisher Scientific). The pDONR/Zeo plasmid containing the sequence encoding Cac1 subunit (Cac1 ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA was isolated from hearts of wild type and mutant mice using the RNAwiz kit (ThermoFisher, #Am1925). The RNA was reverse transcribed by SuperScript reverse transcriptase (Invitrogen) ...
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bioRxiv - Biochemistry 2023Quote: ... The DNA-transfection reagent complex was prepared by combining 4 µg of pcDNA3.1_Mb4.WT (hGOAT) or mutant plasmid and 9 µL Lipofectamine 2000 transfection reagent (Invitrogen) in a total volume of 500 µL supplement free DMEM followed by incubation for 30 min at room temperature ...
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bioRxiv - Neuroscience 2024Quote: Human CSF2RB WT (exons 11 to 14) and A455D mutant donor targeting sequences were cloned into pDONR221 (ThermoFisher) with the following layout (Supplementary Figure S1) ...
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bioRxiv - Biochemistry 2023Quote: The protein (wildtype or Y20A mutant) was pegylated with MS(PEG)12 methyl-PEG-NHS-ester (Thermo Fisher) at 1:30 or 1:60 molar ratio on ice for 2 hours and then proceed to grid freezing ...
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bioRxiv - Cell Biology 2024Quote: Primary human fibroblasts with or without mutant mitochondrial DNA (m.3243G) were routinely maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) containing 25 mM glucose ...
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bioRxiv - Molecular Biology 2024Quote: ... The stable INTS6-GFP and hSSB1-GFP mutants were generated in HeLa WT with Lipofectamine LTX (Invitrogen, 15338100) transfection (2μg plasmid ...
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bioRxiv - Developmental Biology 2024Quote: ... E13.5 or E14.5 control (Shox2dmCherry/+;RosaEYFP/+) or mutant embryos were micro-dissected in 1X DPBS (Gibco, 14190-094) and placed in 1.5ml tubes ...
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bioRxiv - Neuroscience 2021Quote: Retrograde labeling from the CC and the cortical plate were performed by injecting subunit B of cholera toxin (CTB) conjugated to Alexa Fluor 555 (#C-34776, ThermoFisher Scientific. Massachusetts, the U.S.). Injections were performed in the CC ...
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bioRxiv - Neuroscience 2020Quote: ... and axial or intercostal muscles were injected with a solution of Cholera Toxin B Subunit-conjugated Alexa555 (Molecular Probes, Life Technologies, Burlington, ON, Canada), following the manufacturer’s recommendation ...
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bioRxiv - Neuroscience 2019Quote: ... axons of the visual pathway were labeled by intravitreal injection of 2 µl of Alexa Fluor 555-conjugated cholera toxin β subunit (0.5% CTB, in PBS; Molecular Probes, Carlsbad, Eugene, USA) and tissues isolated after another week.
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bioRxiv - Microbiology 2021Quote: ... 50 μl PCR reactions were carried out using 10 ng of sample DNA template and toxin-specific gene primers (Table 3) along with Platinum™ Taq Polymerase (Invitrogen/ThermoFisher, Carlsbad, CA) in accordance with the manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2022Quote: ... All animals were injected with the retrograde tracer Cholera Toxin Subunit B in both the left (Alexa Fluor 647 Conjugate, C34778, Invitrogen, Carlsbad, CA, United States) and right (Alexa Fluor 488 Conjugate ...
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bioRxiv - Neuroscience 2023Quote: P29 mice were anesthetized with isoflurane (1–3%) and 50 nl of the retrograde tracer cholera toxin subunit B (5% wt./vol in PBS, ThermoFisher Scientific, Cat # C-34775) were injected at 10nl/sec into the left SLM (ML ...
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bioRxiv - Developmental Biology 2021Quote: Sperm were isolated from 2-4 wild-type and mutant male fish and kept in 200 μl Hank’s saline containing 0.5 μM MitoTracker Deep Red FM (Molecular Probes) on ice ...
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bioRxiv - Developmental Biology 2021Quote: Sperm were isolated from 2-4 wild-type and mutant male fish and kept in 150 μl Hank’s saline containing 0.5 μM MitoTracker Deep Red FM (Molecular Probes) for >10 minutes on ice ...
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bioRxiv - Developmental Biology 2021Quote: Spermatozoa were squeezed from 2–4 wild-type and mutant male fish and kept in 150 µL Hank’s saline containing 0.5 µM MitoTracker Deep Red FM (Molecular Probes) for >10 minutes on ice ...
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bioRxiv - Developmental Biology 2021Quote: Micro-dissected individual pairs of either control or mutant E12.5 proximal and distal forelimbs were stored at -80°C in RNAlater stabilization reagent (Ambion) before further sample processing ...
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bioRxiv - Cell Biology 2022Quote: ... Full length Cavin1 and ΔDR1 mutants were expressed and purified from suspension cells HEK-293F (Invitrogen, Carlsbad, CA, USA) as described previously (33) ...
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bioRxiv - Molecular Biology 2020Quote: ... HEK293 cells were transfected with either NSUN6 wild-type and the miCLIP-mutant construct using Lipofectamine 2000 (Life Technologies) and harvested 24 hours later.
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bioRxiv - Cell Biology 2019Quote: 70 % of confluence C2C12 cells were transfected with 3 μg GFP lamin mutants by lipofection (Lipofectamine 3000, Life Technologies) following manufacturer instructions ...
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bioRxiv - Cell Biology 2019Quote: ... FLAG-tagged human β2AR with S204/207A double mutations (FLAG-mutant) was generated by Gibson assembly method (Thermo Fisher) using FLAG-β2AR and synthetic gBlocks with the double mutations as templates (Integrated DNA Technologies) ...
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bioRxiv - Genetics 2019Quote: ... Wildtype and mutant ΔN-p73 were amplified and cloned into the Gateway donor vector pDONR221 through BP reactions (Invitrogen). Next ...
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bioRxiv - Plant Biology 2019Quote: RipE1C172A and RipE1 ΔAD mutant variants were generated using the QuickChange Lightning Site-Directed Mutagenesis Kit (Life technologies, USA) following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... plasmids bearing wild type HSA and the mutants were transfected to HeLa cells using Lipofectamine 3000 transfection kit (Invitrogen) and Opti-MEM (Gibco ...
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bioRxiv - Biophysics 2021Quote: ... The genes encoding MICU1 mutants were cloned into a pcDNA3.1(+) vector for transient expression using Lipofectamine 3000 (Life Technologies). Site-directed mutagenesis was performed using a Quick Change kit (Agilent ...
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bioRxiv - Neuroscience 2020Quote: ... and both WT and mutant VAMP2 sequences were shuttled into Gateway™ pDEST™ 26 Vector (6xHis tag, Invitrogen). The in-frame V5-STX1a and 6xHis-(m)VAMP2 sequences were verified by DNA sequencing.
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bioRxiv - Microbiology 2021Quote: ... and the cap59Δ mutant (cap59) strains were incubated in YPD medium at 30°C and CO2-independent medium (Gibco) at 37°C until saturation ...
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bioRxiv - Systems Biology 2020Quote: ... Plasmids containing the expression cassettes containing the wild type and mutant bla gene were transformed into Top10 cells (Invitrogen) and treated with various Amp concentrations as described above.
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bioRxiv - Microbiology 2021Quote: ... were transfected with pcineo plasmids (0.4 μg) carrying the RSV codon-optimised NS1 or FLAG-NS1 WT or mutant constructs using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2021Quote: LGMN KO cells were transiently transfected with pLVX-Hhi3 encoding WT or mutant LGMN genes using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2020Quote: ... retroviral particles were generated by co-transfecting 1.25 μg pBABE-puromycin retroviral vectors expressing WT KRAS or each KRAS mutant with 1.25 μg/μl PCL10A pack vector using 6.25 μg Lipofectamine 2000 (ThermoFisher Scientific) into HEK293T cells seeded in a 6 cm cell culture plate per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Ming-Chih Lai) (50 ng) or mutant MT-STAT1-5’UTR-Luciferase (50 ng) using Lipofectamine 3000 (Life Technologies). In HepAD38 cells ...
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bioRxiv - Microbiology 2021Quote: 400,000 CEM-SS or CEM-T4 cells were infected with 1.6 ng of p24-CA of wildtype or mutant NL4-3 virus in serum-free RPMI medium (Invitrogen) at 37 °C ...
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bioRxiv - Cell Biology 2022Quote: HEK 293T cells were transfected with 1 µg of plasmids containing either FL (wild-type), various deletions (ΔTM, ΔNΔTM, NCC, CC, N) or mutant FLAG-KASH5 constructs with Lipofectamine LTX (Invitrogen) transfection reagent using the manufacturer’s instructions ...