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Citations for Thermo Fisher :
6651 - 6700 of 10000+ citations for 5 Nitrofluorescein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... samples were solubilized in 50 µL of extraction buffer containing 5% sodium dodecyl sulfate (SDS, Affymetrix), 50mM TEAB (pH 8.5 ...
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bioRxiv - Neuroscience 2023Quote: ... FFPE slides of 5 micrometres were prepared with a microtome (Thermo Fisher Scientific, Microm HM 355S).
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bioRxiv - Genomics 2023Quote: Wildtype MCF10A cells (ATCC #CRL-10317) were cultured at 5% CO2 in DMEM/F12 (ThermoFisher, #11320033) supplemented with 5% Horse Serum (ThermoFisher ...
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Cas13d-mediated isoform-specific RNA knockdown with a unified computational and experimental toolboxbioRxiv - Genomics 2023Quote: ... A375 cells were maintained at 37°C with 5% CO2 in DMEM media (ThermoFisher Cat #11965118) supplemented with 10% serum (Serum Plus II ...
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bioRxiv - Microbiology 2023Quote: NaOCl disinfectant was purchased as a solution containing 5% or 50,000 ppm active chlorine (Acros Organics) and stored at 4°C until use ...
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bioRxiv - Microbiology 2023Quote: ... Cells transduced with Lenti-Spike were treated with 5 µg/ml Hoechst solution (Molecular Probes®) before being analyzed under a microscope ...
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bioRxiv - Bioengineering 2023Quote: The primer and template labeled at the 5’-terminus by Cy5 dye were ordered from Invitrogen. The primer-template (P/T-2Cy5 ...
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bioRxiv - Cell Biology 2023Quote: ... Secondary AlexaFluor-conjugated antibodies (Life Technologies; 1:500 in 5% FBS, 0.1% Triton-X in PBS) with Hoechst 33342 dye were incubated for 1 h at room temperature in the dark ...
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bioRxiv - Cell Biology 2024Quote: ... the peptides were sampled on a precolumn (300 μm x 5 mm PepMap C18, Thermo Scientific) and separated in a 200 cm µPAC column (PharmaFluidics ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were boiled for 5 min and loaded into SDS-PAGE gels (Invitrogen, XP04122BOX or NP0322BOX). Western blot membranes were blocked in 5% BSA in TBST (TBS + 0.5% Tween20) ...
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bioRxiv - Genomics 2024Quote: ... 20 µl of 100 mM CaCl2 and 5 µl of 100U MNase (88216, Thermo Fisher Scientific) were added ...
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bioRxiv - Microbiology 2024Quote: ... UK) and BSR-T7/5 cells [44] were cultured in Glasgow minimal essential medium (GMEM, Gibco), supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2024Quote: ... anti-GPIX antibodies (5 µg/ml) and AlexaFluor647-labeled human fibrinogen (Life Technologies, 100 µg/ml) were added to WB ...
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bioRxiv - Cell Biology 2024Quote: SUM159 cells69 were cultured at 37°C and 5% CO2 in DMEM/F12 (Corning or Gibco), supplemented with 5% FBS (Gibco) ...
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bioRxiv - Molecular Biology 2024Quote: ... the peptides were sampled on a precolumn (300 μm x 5 mm PepMap C18, Thermo Scientific) and separated in a 200 cm µPAC column (PharmaFluidics) ...
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bioRxiv - Biophysics 2024Quote: ... and full-length MLC-5 were purified from mammalian cultured cells (FreeStyle 293-F cell, Invitrogen). For purifying hsDHC-1382k ...
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bioRxiv - Bioengineering 2024Quote: EVs were labeled using either a 5 µM solution of CellTraceTM Far Red (Thermo Fisher Scientific) or 5(6)-carboxyfluorescein diacetate N-succinimidyl ester (CFDA-SE ...
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bioRxiv - Microbiology 2024Quote: ... Fecal supernatant (140 µL) was treated with 5 units of Pierce™ Universal Nuclease (ThermoFisher Scientific) for 10 minutes at room temperature prior to viral DNA extraction to remove free DNA/RNA molecules ...
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bioRxiv - Cancer Biology 2024Quote: ... The macrosections were stained with 0.7 µL of DAPI (5 µg/mL, Thermo Scientific, Cat#: 62248) and 5 µL of each fluorescently labeled anti-CK8 ...
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bioRxiv - Genetics 2024Quote: ... cell lysates were pre-cleared with 5 µl of unbound protein G magnetic beads (10004D, Invitrogen) for 30 min with rotation at 4°C ...
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bioRxiv - Immunology 2024Quote: Cells were stimulated with ultrapure LPS (5 ng/ml unless specified otherwise) (E.coli 0111:B4, Invitrogen), recombinant human IFN-γ (2 ng/ml ...
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Autophagy suppression in DNA damaged cells occurs through a newly identified p53-proteasome-LC3 axisbioRxiv - Cell Biology 2024Quote: ... 20 μL (approximately 5% of total immunoprecipitation volume) of pre-washed Protein A magnetic Dynabeads (Invitrogen) were added to each lysate sample ...
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bioRxiv - Cancer Biology 2024Quote: A 5-ethynyl-2′-deoxyuridine (EdU) incorporation assay was performed according to the manufacturer’s protocol (Invitrogen). Hydrogel-embedded PCLS were incubated for 16 hours in complete culture media with 10 μM EdU ...
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bioRxiv - Microbiology 2024Quote: ... Amplifications were achieved using a 3-step program on a QuantStudio 5 system (Thermo Fisher Scientific). Transcript abundance was calculated using the ΔΔCT method (75 ...
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bioRxiv - Neuroscience 2024Quote: ... Then 2-5 µg of RNA were reverse transcribed using the Superscript III reverse transcriptase (Invitrogen) and oligo dT primer (Invitrogen) ...
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bioRxiv - Cancer Biology 2024Quote: ... was used to perform qPCR in triplicates using the QuantStudio 5 Real-Time PCR System (ThermoFisher). Data were normalized against Gapdh.
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bioRxiv - Cancer Biology 2024Quote: hiPSCs were cultured at 37 °C and 5% CO2 on Geltrex-coated (Thermo Fisher Scientific, #A1413301) culture plates in mTeSR Plus (Stemcell Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... and were passaged in aggregates every 3-5 days using Versene solution (Thermo Fisher Scientific, #15040066) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... and pVSV-G (390 ng) packaging plasmids in 5 × 105 HEK293T cells with Lipofectamine 2000 (Invitrogen). The supernatant was collected 40 hours later ...
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bioRxiv - Molecular Biology 2024Quote: Unfragmented UHRR or HEK-293T RNAs (5 μg) were DNased with 2 U TURBO DNase (Invitrogen) and purified using an RNA Clean & Concentrator kit (Zymo Research ...
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bioRxiv - Bioengineering 2024Quote: ... This mixture was heated at 95 °C for 5 min using a heating block (Thermo Scientific). The samples and the premixed molecular weight markers (ladder ...
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bioRxiv - Molecular Biology 2024Quote: ... Fluorescence melt curve data was acquired using a QuantStudio 5 Real-Time PCR System (Applied Biosystems) with the following method ...
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bioRxiv - Molecular Biology 2024Quote: ... Semi-quantitative reverse transcription PCR was done on a QuantStudioTM 5 Real-Time PCR (Thermofisher ScientificTM) with specific Taqman probes targeting the genes of interest using the TaqMan Fast Advanced Master Mix for qPCR (Applied Biosystems #4444963) ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were incubated at 37°C and 5% CO2 and split using 0.25% Trypsin (Gibco #15090046) when 80% confluency was reached.
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bioRxiv - Neuroscience 2024Quote: ... The fixed cells were then blocked with 5% normal goat serum (NGS) (Thermofisher Scientific, Cat# 31872) for 1 h at room temperature ...
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bioRxiv - Physiology 2024Quote: ... samples were blocked in PBS+0.3% Triton-X with 5% normal goat serum (NGS, Thermo Fisher) at room temperature for 2 hours ...
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bioRxiv - Neuroscience 2024Quote: ... Quantitative real-time PCR was conducted using a QuantStudio 5 Real-Time PCR System (ThermoFisher Scientific). All samples were run in triplicate ...
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bioRxiv - Immunology 2024Quote: ... IL-5 levels were quantified in undiluted BAL fluid by ELISA following the manufacturer’s instructions (Invitrogen).
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bioRxiv - Neuroscience 2024Quote: ... Neurons were imaged using the Helios 5 Hydra CX Dual Beam system (Thermo Scientific, The Netherlands) operating at 2kV and 0.4 nA beam current ...
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bioRxiv - Genomics 2024Quote: ... A 25 cm2 flask was pre-coated with 5 μg/mL Laminin (ThermoFisher, cat. no. 23017015) in PBS for 8 - 12 hours ...
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bioRxiv - Biochemistry 2024Quote: ... 5% (v/v) glycerol) then was loaded onto a POROS™ HS 20 µm column (ThermoFisher) and eluted using a 200 to 1000 mM NaCl gradient ...
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bioRxiv - Genetics 2024Quote: ... Fungal nuclei were stained 5 min before imaging with 2 µg/ml Hoechst 33342 (Invitrogen™) in water in the dark ...
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bioRxiv - Molecular Biology 2024Quote: Confluent HFF cells were infected with ME49ΔKu80 parasites (1 parasite: 5 hosts) in DMEM media (Invitrogen) with 10% (v/v ...
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bioRxiv - Neuroscience 2024Quote: ... then 5 μL samples were used to coat an ELISA plate (Nunc, MediSorp, Thermo Fisher Scientific) overnight at 4°C with agitation ...
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bioRxiv - Cancer Biology 2024Quote: ... in the presence of 5 μg/mL Brefeldin A (BFA, Thermo Fisher Scientific, #00-4506-51) and 5 μg/mL monensin (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... Cells were maintained at 37 □C and 5% CO2 in Dulbecco’s Modified Eagle medium (DMEM; Gibco) supplemented with 10% fetal calf serum ...
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bioRxiv - Cell Biology 2024Quote: ... and 5% CO2 and were passaged twice per week using DBPS (Gibco, Cat no. 14190-094) and Trypsin-EDTA (Gibco ...
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bioRxiv - Systems Biology 2024Quote: ... Colons were collected within 5 min of death and flushed using ice-cold 1x PBS (Gibco) using a straight gauge needle with a 2.4mm tip (Kent Scientific Corporation ...
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bioRxiv - Systems Biology 2024Quote: ... Plasmids went through an additional step of 5’ dephosphorylation using FastAP Thermosensitive Alkaline Phosphatase (Thermofisher, EF0654) to avoid self ligation ...
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β-1,6-glucan plays a central role in the structure and remodeling of the bilaminate fungal cell wallbioRxiv - Microbiology 2024Quote: ... After 16 h incubation at 37°C in a 5% CO2 chamber (Cytoperm 2, ThermoFisher Scientific), the culture supernatants were collected and stored at –20°C until further analysis.