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Citations for Thermo Fisher :
6451 - 6500 of 10000+ citations for 5 Nitrofluorescein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... Fluorescence melt curve data was acquired using a QuantStudio 5 Real-Time PCR System (Applied Biosystems) with the following method ...
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bioRxiv - Genetics 2024Quote: ... samples were solubilized in 50 µL of extraction buffer containing 5% sodium dodecyl sulfate (SDS, Affymetrix), 50mM TEAB (pH 8.5 ...
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bioRxiv - Microbiology 2023Quote: ... and cDNA was synthesized from 5 µg RNA with the SuperScript VILO cDNA synthesis kit (Invitrogen). Real-time qPCR was performed using a LightCycler®480 Real-Time PCR system (Roche Diagnostics ...
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bioRxiv - Cell Biology 2024Quote: ... and reversed transfected at a concentration of 5 nmol/L using Lipofectamine RNAiMAX transfection reagent (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: ... Cells were collected into fluorescence-activated sorting tubes and co-stained with 5 µM 7AAD (Invitrogen) and 1X BD Pharmingen™ PE Annexin V (BD Biosciences ...
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bioRxiv - Cell Biology 2024Quote: ... Complete culture media included media supplemented with 5% (v/v) Fetal Bovine Serum (FBS, Life Technologies) and 1% (v/v ...
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bioRxiv - Molecular Biology 2023Quote: ... after the addition of cel-miR-39 spike-in for normalization (5 pmol/µl) (ThermoFisher Scientific). MicroRNAs and U6 small nuclear RNA (supplementary table 2 ...
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bioRxiv - Bioengineering 2023Quote: The primer and template labeled at the 5’-terminus by Cy5 dye were ordered from Invitrogen. The primer-template (P/T-2Cy5 ...
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bioRxiv - Molecular Biology 2024Quote: ... Hybridization was performed using 5’-labeled oligonucleotides atpB (CC2437) and glnA (CC2438) using Ultra-Hyb (Ambion) hybridization buffer at 42°C for a minimum of 4 hours ...
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bioRxiv - Immunology 2023Quote: ... Anti-flavivirus E protein mAb 4G2 was conjugated to Alexa Fluor 488 5-SDP (Life Technologies) following the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: GL261 cells (4 × 107/mL) were incubated with 5 μM CFSE solution (Invitrogen Molecular Probes, USA) diluted in PBS for 15 min at 37 °C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: GL261 cells (4 × 107/mL) were incubated with 5 μM CFSE solution (Invitrogen Molecular Probes, USA) diluted in PBS for 15 min at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... previously preincubated overnight with 5% BSA and 667 µg/ml Ultra-Pure salmon sperm DNA (Invitrogen), and incubated for 3 h at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... The fecal supernatant (140 µL) was treated with 5 units of Pierce Universal Nuclease (ThermoFisher Scientific) for 10 minutes at room temperature prior to viral DNA extraction to remove free DNA/RNA molecules ...
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bioRxiv - Microbiology 2024Quote: ... 5 μl of DNase I buffer (Fermentas) and 0.5 μl of Superase In (Thermo Fisher Scientific) were added to the denatured RNA and incubated at 37°C for 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... Nucleus were stained for 5 min with Hoechst n°33342 (ref H3570, Invitrogen, Carlsbad, California, USA) at 1:2000 ...
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bioRxiv - Cancer Biology 2024Quote: ... were obtained from American Type Culture Collection (ATCC) and cultured at 37°C and 5% CO2 in either RPMI-1640 (Gibco 11835-030) or Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Cell Biology 2024Quote: ... qRT-PCR was performed on a QuantStudio 5 Real-Time PCR System (Applied Biosystems, Waltham, MA) using KAPA SYBR FAST qPCR Master Mix (Kapa Biosystems ...
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bioRxiv - Developmental Biology 2024Quote: ... Intracellular ROS was measured by incubating oocytes or follicles with 5 µM CM-H2DCFDA (C6827, Invitrogen). Mitochondrial ROS was measured by incubating oocytes with 500 nM MitoSOX Red (M36008 ...
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bioRxiv - Developmental Biology 2024Quote: ... organoids were further digested for 5 min with Trypsin + 0.25% EDTA (Gibco, Catalog No. 25200-072) at 37 °C and pipetted until the obtention of a single cell suspension ...
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bioRxiv - Developmental Biology 2024Quote: ... and cells were passaged every 4-5 days using 0.5 mM EDTA (Life Technologies, #15575-020) to dissociate cells maintaining clumps ...
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bioRxiv - Immunology 2024Quote: ... CHO cells were kept at 37°C and 5% CO2 in OptiMEMTM with GlutamaxTM (Life Technologies) supplemented with 10% heat-inactivated endotoxin-low FCS ...
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bioRxiv - Genetics 2024Quote: ... we mixed 5 µL of PCR product with 1 µL of 6X loading dye (ThermoFisher Scientific) and ran samples on 2% agarose gels stained with RedSafe (FroggaBio ...
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bioRxiv - Immunology 2024Quote: ... Residual red blood cells were lysed by 5 mL of ACK lysing buffer (Gibco, Waltham, MA) for 10 mins ...
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bioRxiv - Biochemistry 2024Quote: HEK293T cells were grown at 37°C in a 5% CO2 atmosphere in DMEM (Gibco 11995065), supplemented with 10% FBS ...
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bioRxiv - Biochemistry 2024Quote: ... were all grown at 37°C in 5% CO2 using DMEM/F-12 medium (Gibco, #10565042) supplemented with 5% horse serum (Gibco ...
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bioRxiv - Cell Biology 2024Quote: FlpIn CHO and HEK293 cell lines were purchased from Invitrogen and maintained in Dulbecco’s Modified Eagle’s Medium supplemented with 5% Foetal Bovine Serum (ThermoFisher Scientific (Gibco)) at 37°C ...
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bioRxiv - Bioengineering 2024Quote: ... qRT-PCR was then performed using the QuantStudio 5 Real-Time PCR system (Thermo Fisher Scientific). ΔCt values were calculated using GAPDH and compared between treatment groups ...
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bioRxiv - Bioengineering 2024Quote: ... IVT was then performed using MEGAscript® T7 Transcription Kit (Thermo Fisher Scientific, AMB-1334-5) according to the manufacturer’s instructions with modifications as using the CleanCap® Reagent AG (TriLink ...
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bioRxiv - Biophysics 2024Quote: ... The trapping column was PEPMAP NEO C18 (5 µm particle size, 300 µm*5mm, Thermo Scientific). Analytical column was nano EaseTM M/Z HSS C18 T3 (100Å ...
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bioRxiv - Cell Biology 2024Quote: HL-60 cells were cultured with IMDM containing 20% FBS (Gemini) and 5% Penicillin-Streptomycin (Gibco) and maintained at 1 x 105 and 1 x 106 cells/ml ...
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bioRxiv - Cell Biology 2024Quote: FlpIn CHO and HEK293 cell lines were purchased from Invitrogen and maintained in Dulbecco’s Modified Eagle’s Medium supplemented with 5% Foetal Bovine Serum (ThermoFisher Scientific (Gibco)) at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were selected and maintained in the presence of 5 μg/mL blasticidin (Thermo Fisher, MA). Monoclonal cells were isolated from a polyclonal pool of transduced stable cells using the limiting dilution assay ...
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bioRxiv - Neuroscience 2023Quote: ... the frozen sections were blocked with 5% normal goat serum (NGS) (Thermo Fisher Scientific, Cat# 31872) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... 15-20 pooled day 30 neurospheres and 5-7 pooled day 60 organoids using TRIzol (Invitrogen; Thermo Fisher Scientific Inc. ...
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bioRxiv - Cell Biology 2023Quote: ... Secondary AlexaFluor-conjugated antibodies (Life Technologies; 1:500 in 5% FBS, 0.1% Triton-X in PBS) with Hoechst 33342 dye were incubated for 1 h at room temperature in the dark ...
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bioRxiv - Biochemistry 2024Quote: ... at 37°C under 5% CO2 in Dulbecco’s Modified Eagle’s Medium (Gibco, Cat. no: 12800-017) supplemented with 10% FBS (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... and incubated with Alexa488-labelled goat anti-mouse IgG (A11001, Thermo Fisher Scientific, 5 μg/mL) together with Alexa594-labelled phalloidin (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... Up to 5 μg RNA was used for cDNA synthesis by RevertAid reverse transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... Peptides were separated onto a 300 μm × 5 mm PepMap C18 trapping column (Thermo Scientific, Lithuania) followed by DNV PepMap Neo 75umx150mm analytical column (Thermo Scientific ...
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bioRxiv - Microbiology 2023Quote: ... using a CuAAC reaction cocktail (0.2mL) containing 5 μM Alexa Fluor 488 azide (Thermo Fisher, USA), 1 mM CuSO4(aq ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA (1 μg) was ligated to the 5’-phosphorylated RNA adaptor using T4 RNA Ligase (Ambion). Following ligation ...
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bioRxiv - Neuroscience 2023Quote: ... PBS washes (3 × 5 min) and incubation with primary antibody (mouse anti-GFP, A11120, Life technologies; 1:200 ...
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bioRxiv - Microbiology 2023Quote: ... 5-10 μg of total RNA in Gel Loading Buffer II (GLII, Ambion; 95% (v/v) formamide ...
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bioRxiv - Synthetic Biology 2022Quote: ... Culture of transformed sensors (5 μL) was plated onto a 12-well plate (Thermo Fisher Scientific) for selection ...
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bioRxiv - Microbiology 2022Quote: ... 293T cells were maintained at 37°C and 5% CO2 in DMEM (Gibco catalog number 11875093) supplemented with 10% fetal bovine serum (ThermoFisher catalog number 11965084 ...
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bioRxiv - Bioengineering 2023Quote: ... followed by 1 h incubation in the blocking buffer (5% Fetal Bovine Serum (FBS, Gibco, USA) and 1% Bovine Serum Albumin (BSA ...
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bioRxiv - Systems Biology 2023Quote: ... the medium was changed to serum-starved medium supplemented with 5 µg/mL heparin (Thermo Scientific). After 24 hours ...
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bioRxiv - Systems Biology 2023Quote: ... Samples were centrifuged for 5 min at top speed in a Heraeus Pico21 centrifuge (Thermo Scientific). The organic phase ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 µg RNA was incubated with washed beads (washing buffer: 300 mM NaCl [#AM9760G; Thermo Fisher Scientific] ...