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Citations for Thermo Fisher :
6501 - 6550 of 10000+ citations for 5 Nitrofluorescein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... supplemented with L-glutamine (4 mM) and 5% foetal bovine serum (Thermo Scientific, Waltham, MA, USA). Cell culture medium was supplemented with 0.1 mM NaI and 1 mM NaBr (to model physiological availability of iodine and bromide) ...
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bioRxiv - Cell Biology 2023Quote: ... Cybrid cells were cultured at 37⁰C and 5% CO2 in DMEM high glucose (25mM, Gibco) medium supplemented with sodium pyruvate (1mM) ...
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bioRxiv - Cancer Biology 2023Quote: ... with a final protein concentration of 5 μΜ and the fluorescent dye SYPRO Orange (Invitrogen, 10338542) at a dilution of 1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... HeLa cells (ATCC) were cultured at 37°C with 5% CO2 in DMEM + GlutaMAX medium (GIBCO) supplemented with 10% heat-inactivated fetal bovine serum ...
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bioRxiv - Microbiology 2023Quote: ... Sf1Ep cells were incubated in a 5% CO2 atmosphere in a HeraCell 150 incubator (Thermo Fisher). Following treponemal cell inoculation ...
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bioRxiv - Microbiology 2022Quote: ... 5 mM or 20 mM sodium butyrate using the Ambion Ribo-Pure Bacteria Kit (ThermoFisher Scientific). The RNA samples were subsequently DNase I treated ...
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bioRxiv - Molecular Biology 2023Quote: ... the peptides were sampled on a precolumn (300 μm x 5 mm PepMap C18, Thermo Scientific) and separated in a 75 μm x 250 mm C18 column (PepMap C18 ...
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bioRxiv - Microbiology 2023Quote: ... followed by lipid-staining with 20 μL of 5 μg/mL FM4-64 (Thermo Fisher Scientific) at 20 °C for 30 min in the dark ...
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bioRxiv - Immunology 2022Quote: ... Tumor cell lines were cultured at 37°C and 5% CO2 in culture media [DMEM (Gibco) supplemented with 10% heat-inactivated FBS (Atlanta Biologicals) ...
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bioRxiv - Genomics 2023Quote: ... This mixture was incubated at 65°C for 5 min in an ABI2700 thermocycler (Applied Biosystems) then ramped down to 4°C ...
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bioRxiv - Developmental Biology 2023Quote: The medusae were incubated with 150 μM 5-ethynyl-2’-deoxyuridine (EdU) (EdU kit; Invitrogen, C10337) in ASW for 1 h or 24 h ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were cultured in DME/F12 media supplemented with 5% horse serum (Thermo Fisher Scientific, 16050122), 20 ng/mL human Epidermal Growth Factor (hEGF ...
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bioRxiv - Genomics 2023Quote: ... This mixture was incubated at 65°C for 5 min in an ABI2700 thermocycler (Applied Biosystems) then ramped down to 4°C ...
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bioRxiv - Physiology 2023Quote: ... larval fat bodies at 5 and 12 days AEL were dissected in Schneider’s S2 medium (GIBCO). After incubation in 30 μM DHE (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... 5 µl of ligation product was transformed into 50 µl of Stbl3 competent cells (Invitrogen, #C737303). Insertion of guide RNA into lentiviral CRISPR plasmid was verified by Sanger sequencing under U6-forward primer (5’-GACTATCATATGCTTACCGT-3’ ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were split every 5-7 days by incubation with 0.5 μM EDTA (Thermo Fisher Scientific) for 4 min at room temperature and clumps were dissociated into small clumps by pipetting ...
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bioRxiv - Microbiology 2023Quote: ... Ten μL reaction volumes containing 5 μl SYBR Select Master Mix (Life Technologies, Grand Island, NY), 400 nM of primer attR-F and attL-R (Table 2) ...
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bioRxiv - Microbiology 2023Quote: ... based on a similar strategy previously reported [5] but using the Phi29 DNA Polymerase (Thermo Scientific) and newly designed primers (Table S1) ...
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bioRxiv - Neuroscience 2023Quote: ... AF 488 rat anti-mouse TMEM119 (cat # 53-6119-82, Invitrogen, clone V3RT1GOsz, 5 ng/μl), and Alexa 488 rat anti-mouse NeuN (cat # MAB377X ...
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bioRxiv - Neuroscience 2023Quote: Stably expressing 5-HT receptor Flp-In 293 T-Rex Tetracycline inducible system (Invitrogen, mycoplasma-free) were used for calcium flux assays ...
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bioRxiv - Neuroscience 2023Quote: ... followed by an approximately 5 minute incubation with EDTA (0.5 mM, pH 8.0, Thermo Fisher Scientific) for maintenance or TrypLE (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were grown at 37°C and 5% CO2 in DMEM high glucose medium (Gibco, #11965092) supplemented with 10% FBS (Corning Cellgro ...
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bioRxiv - Physiology 2023Quote: ... R: 5’ TGCCTTCGTGCCGTACTG 3’) and confirmed by DNA sequencing following sub-cloning into pCRTM-Blunt (Invitrogen). Further genotypes were determined by real-time PCR with specific probes designed for each gene (Transnetyx ...
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bioRxiv - Microbiology 2023Quote: BSRT7/5 cells at 90% confluence in 96-well dishes were transfected using Lipofectamine 2000 (Invitrogen) with a plasmid mixture containing 62.5 ng of pGaussia/Firefly minigenome ...
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bioRxiv - Systems Biology 2023Quote: ... membranes were incubated for 5 min in SuperSignal West Pico PLUS chemiluminescent substrate (Thermo Fisher, 34579) according to manufacturer’s instruction just prior to detection on a Bio-Rad ChemiDoc ...
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bioRxiv - Systems Biology 2023Quote: ... and transferred to a conical tube containing 5 mL of 1X CD CHO Medium (Gibco, 10743011) bubbled with carbogen ...
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bioRxiv - Synthetic Biology 2023Quote: ... Samples (using 25 μL each) including 5 μL PageRuler Plus (Thermo Fisher Scientific, Waltham, MA, USA) or Precision Plus Dual Xtra (Bio-Rad ...
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bioRxiv - Developmental Biology 2023Quote: ... washed with PBS three times (5 min each) and mounted with Shandon Immuno-Mount (Thermo Scientific). The antibodies and dilutions employed for cell immunofluorescence were the following ...
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bioRxiv - Plant Biology 2023Quote: ... Further add 0.5 µl of NativePAGE 5% G250 sample additive (Invitrogen, Thermo Fisher Scientific, Carlsbad, CA) to the 19.5 µL of the previous mixture and mix it well ...
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bioRxiv - Biochemistry 2023Quote: ... the lipid mixture was supplemented with 5 mol% of R18 (100 mg/ml in DMSO; Invitrogen). Fluorometry experiments were performed using a FP-8500 spectrofluorometer (Jasco ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... The qPCR was run in duplicate using a QuantStudio 5 system (Applied Biosystems, Thermo Fisher Scientific), and the 20 µl reactions consisted of 10 µl Power SYBR Green PCR Master Mix (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 μg of sgRNA plasmid was transfected to PLC/PRF/5 cells using Lipofectamine 3000 (Invitrogen). After 2 days’ culture in DMEM medium ...
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bioRxiv - Cell Biology 2023Quote: ... TMT reaction was allowed for 2 hours and quenched with 5% hydroxylamine (90115, Thermo Fisher Scientific). All the samples were pooled and dried in a SpeedVac (EP022822993 ...
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bioRxiv - Cell Biology 2023Quote: Cells were cultured in media containing 5 μg/ml Hoechst 33342 Solution (Thermo Fisher Scientific, 62249) at 37°C for 30 - 60 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... an aliquot (30 μL) of resuspended beads and anti-Tau-5 antibody (Thermo Fisher, 1:250) were added to prepared lysates and incubated at 4 °C overnight with rotation ...
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bioRxiv - Neuroscience 2023Quote: ... and then blocked for two hours in 5% BSA/PBS (BP1600, Fisher Scientific, Fair Lawn, NJ) at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... Sample was loaded onto the trapping column (Thermo Scientific, PepMap100, C18, 300 um x 5 mm), using partial loop injection for 3 min at a flow rate of 15 µL/min with 0.1% (v/v ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were cultured (37°C, 5% CO2) in growth medium containing DMEM (Thermo Fisher Scientific, 11965092) with 10% Tet System Approved heat-inactivated FBS (Takara Bio ...
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bioRxiv - Molecular Biology 2023Quote: ... Each 10 µl reaction contained 5 µl SYBR GreenER™ qPCR SuperMix Universal (Thermo Fisher Scientific), forward and reverse primer at a concentration of 200 nM each ...
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bioRxiv - Immunology 2023Quote: ... 20-30 µg of proteins were resuspended in 5× Lane Marker Reducing Sample Buffer (Thermo Fisher), boiled for 5 min at 100°C ...
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bioRxiv - Neuroscience 2023Quote: ... the cells were lysed for 5 min with M-PER mammalian protein extraction reagent (Thermo Scientific) including Halt protease inhibitor (Thermo Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... Dissociated DRG were filtered (100µM, Fisher) and cultured in DRG media [5% fetal bovine serum (Gibco) and 1% penicillin/streptomycin (Corning ...
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bioRxiv - Neuroscience 2023Quote: ... 5 to 50mg of tissue was ground in N-PER lysis buffer (ThermoFisher Product No. 23225) with protease and phosphatase inhibitors (cOmpleteTM Protease Inhibitor Cocktail 11697498001 ...
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bioRxiv - Microbiology 2023Quote: ... and boiled for 5 min before separation on a 4-12% NuPAGE Bis-Tris gel (Invitrogen) for immunoblotting.
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bioRxiv - Genetics 2023Quote: ... The amplification was performed in a QuantStudio 5 Real-Time PCR System (Thermo Fisher Scientific, USA). Samples were amplified using RT2 SYBR® Green qPCR Mastermix with ROX (carboxy-X-rhodamine ...
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bioRxiv - Microbiology 2023Quote: ... and stained with 5 μg mL−1 Alexa Fluor 568 donkey anti-rabbit IgG (Invitrogen, A10042) for 1 h at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... the peptides were sampled on a precolumn (300 μm x 5 mm PepMap C18, Thermo Scientific) and separated in a 75 μm x 250 mm C18 column (Reprosil-Pur 120 C18-AQ ...
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bioRxiv - Molecular Biology 2023Quote: ... female) were grown at 37°C and 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM, Invitrogen) supplemented with fetal calf serum (EUROBIO ...
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bioRxiv - Pathology 2023Quote: ... Mice were perfused via the left ventricle with 5 mL of ice cold HBSS (14025092, Gibco) after which perfused kidneys were collected and placed on ice in HBSS ...
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bioRxiv - Plant Biology 2023Quote: ... the peptides were sampled on a precolumn (300 μm x 5 mm PepMap C18, Thermo Scientific) and separated in a 75 μm x 250 mm C18 column (Aurora Generation 2 ...