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Citations for Thermo Fisher :
6601 - 6650 of 10000+ citations for hsa mir 23b Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... Reactions were assembled in MicroAmp Optical 96-well reaction plates and carried out in a QuantStudio-7 Flex real-time PCR instrument (Applied Biosystems). PCR conditions were as follows ...
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bioRxiv - Neuroscience 2022Quote: ... Gene expression was quantified by real-time PCR with marmoset specific TaqMan Gene Expression Assays (Applied Biostems) and TaqMan Fast Advanced Master Mix (Applied Biosystems) on a QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems).
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bioRxiv - Cancer Biology 2022Quote: ... qPCR was conducted in a Bio-Rad CFX96 real-time PCR system using TaqMan gene-specific primer/probe sets (Applied Biosystems) and SsoAdvanced master mix (Bio-Rad) ...
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bioRxiv - Microbiology 2022Quote: ... The qPCR plate was sealed and read in the FAM and HEX channels using a QuantStudio™ 5 Real-Time PCR system (ThermoFisher) under the following cycling protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... A final cDNA concentration of 5ng/µl was used for semi-quantitative real time PCR analysis performed in Thermocycler ABI7500 Fast (Applied biosystems), using PowerUp SYBR Green Master Mix (Applied biosystems ...
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bioRxiv - Biophysics 2022Quote: The structural integrity of the protein at increasing temperature was performed using SYPRO Orange as external fluorophore in a 7500 Real-Time PCR System (Applied Biosystems). 20□μl samples were prepared at 5x SYPRO Orange fluorophore (Sigma) ...
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bioRxiv - Cancer Biology 2022Quote: ... mixed with indicated cDNAs and primers (primer sequences are listed in Supplemental Table S1) in a QuantStudio Real-Time PCR system (Applied Biosystems). Expression levels were normalized by 18S rRNA.
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bioRxiv - Cell Biology 2022Quote: ... Genotyping was performed using KASPar assays (LGC Genomics) in an Applied Biosystems 7500 Fast Real-Time PCR system (Thermo Fisher Scientific), according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... The transcripts of lysosome biogenesis genes and their transcription factors were amplified from the cDNA in QuantStudio 6 Flex real-time PCR system (Applied Biosystems). As a control 18S rRNA or β-Actin were amplified along with the lysosomal genes ...
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bioRxiv - Plant Biology 2021Quote: ... Quantitative real-time PCR reactions were performed using Brilliant III Fast SYBR-Green QPCR Master Mix on an QuantStudio 3 real-time PCR instrument (Life Technologies) with the following program ...
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bioRxiv - Neuroscience 2019Quote: ... in the three brain regions was measured using a StepOne Plus real-time thermocycler with a Fast SYBR Green PCR master mix (4385612; Applied Biosystems). Primers were purchased from Qiagen or Eurofins Genomics and designed according to GenBank sequence information at the National Center for Biotechnology Information (NCBI ...
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African Swine Fever Virus CD2v protein promotes β-Interferon expression and apoptosis in swine cellsbioRxiv - Microbiology 2020Quote: ... IFN-β and ISGs mRNAs were quantified using ABI and QuantStudio-3 Real time PCR system (Applied Biosystems, Foster city, CA), Power SYBR Green PCR Master Mix (Applied Bio ...
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bioRxiv - Cancer Biology 2021Quote: ... The cDNA was then used as templates and qPCR was performed using the SYBR Green qPCR Master Mix (SolomonBio, QST-100) on the QuantStudio 3 Real-Time PCR system (Applied Biosystems). Primers used in qPCR were listed in Supplementary Table 8.
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bioRxiv - Immunology 2020Quote: ... Real-time PCR was performed using an UltraSYBR Mixture (Beijing CoWin Biotech, Beijing, China) on a ViiA 7 real-time PCR system (Applied Biosystems). Viral rRNAs were normalized to swine 28S rRNA expression ...
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bioRxiv - Cancer Biology 2020Quote: ... Data analysis was performed with the QuantStudio 6 and 7 Flex Real-Time PCR System Software v1.0 (Applied Biosystems, Life Technologies) using the ΔΔCt method.
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bioRxiv - Cell Biology 2021Quote: ... The resulting cDNA was amplified quantitatively using PerfeCTa SYBR Green Mix (Quanta) on a 7500 Fast Real-time PCR System (Applied Biosystems). The relative gene expression was determined using differences in Ct values between gene of interest and house-keeping control genes β-actin and HPRT1.
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bioRxiv - Biochemistry 2021Quote: Thermal unfolding experiments were carried out through preferential binding of a fluorophore to unfolded protein using a ViiA7 real-time PCR instrument (Applied Biosystems). In brief ...
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bioRxiv - Microbiology 2021Quote: RT-qPCR assays were performed using 5x HOT FIRE Pol EvaGreen qPCR Mix Plus (Solis Biodyne) in a QuantStudio 3 Real-Time PCR System (ThermoFisher Scientific) machine ...
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bioRxiv - Neuroscience 2021Quote: ... Cx3cr1 and 18S RNA (internal control) were measured by semiquantitative real-time PCR on an ABI Prism 7900 HT (Applied Biosystems).
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bioRxiv - Biochemistry 2021Quote: ... Murine PNPLA2 mRNA levels relative to HPRT transcript levels were measured by the QuantStudio 7 Flex Real-Time PCR System using Taqman® gene expression assays (PNPLA2, Mm00503040_m1; HPRT, Mm00446968_m1, Thermo Fisher Scientific). PNPLA2 relative expression to HPRT was calculated using the comparative ΔΔCt method.27
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bioRxiv - Microbiology 2021Quote: Real-time PCR was performed using primers (Integrated DNA Technologies, Coralville, IA, USA) and probes (Thermo Fisher Scientific, Waltham, MA, USA) with sequences and concentrations listed in Table 1 in combination with QuantiTect Multiplex PCR Kit (Qiagen ...
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bioRxiv - Cancer Biology 2020Quote: ... Data analysis was performed with the QuantStudio 6 and 7 Flex Real-Time PCR System Software v1.0 (Applied Biosystems, Life Technologies) using the ΔΔCt method.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... analysis was performed using a QuantStudio 12 K Flex real-time PCR system (Applied biosystem) and Luminaris Probe qPCR Master Mix (Thermo Scientific), following the manufacturers’ instructions ...
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bioRxiv - Immunology 2022Quote: ... The reactions were set up using the manufacturer’s guidelines and run on a StepOnePlue Real-Time PCR Machine (Thermo Fisher Scientific). Values are represented as the difference in cycle threshold (Ct ...
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bioRxiv - Immunology 2022Quote: ... cDNA was amplified using the Fast SYBR Green Master Mix on a QuantStudio 7 Flex Real Time PCR Systems (Applied Biosystems). The sequences of primer pairs (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... and Il-1β (in aortic CD3- cells) mRNA levels were assessed by qPCR using QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems), and initial differences in RNA quantity were corrected for using the housekeeping genes 36b4 and Cyclophilin B.
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bioRxiv - Developmental Biology 2022Quote: ... with cDNA (diluted 1:5) and gene-specific primers (Key Resources Table) on a QuantStudio 3 Real-Time PCR System (Applied Biosystems) in triplicate ...
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bioRxiv - Developmental Biology 2022Quote: ... with cDNA (diluted 1:10) and gene-specific primers (Key Resources Table) on a QuantStudio 3 Real-Time PCR System (Applied Biosystems) in triplicate ...
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bioRxiv - Synthetic Biology 2022Quote: ... PCR was performed for 55-60 cycles for maximal saturation of signal with final extension at 72 °C for 7 min in a 7500 Fast Real-Time PCR System (Applied Biosystems). The primer sequences used were ...
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bioRxiv - Plant Biology 2019Quote: ... was performed in triplicate on a 96-well plate in the Step-One Plus Real-Time PCR system (Applied Biosystems, USA). The melon Cyclophilin A gene (Melo3C013375 ...
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bioRxiv - Genomics 2020Quote: ... The cDNA of all target genes was amplified for 50 cycles and quantified with a SYBRGreen-based real-time PCR (Applied Biosystems) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... which was performed with the Applied Biosystems 7500 Fast Real-Time PCR System using Power SYBR Green Master Mix (Applied Biosystems) and qRT-PCR primers (listed in Supplementary table S1) ...
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bioRxiv - Molecular Biology 2019Quote: ... analysis was performed using ABI MicroAmp Optical 384-well Reaction plates on an ABI 7900 real-time PCR instrument (Applied Biosystems). The qPCR was performed using TaqMan Gene Expression Assays that consisted of forward and reverse primers with TaqMan minor groove binder (MGB ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and Gnat2 (a cone-specific gene) was measured from retinal cDNA using custom probe based quantitative real-time PCR (qPCR) assays (Taqman® probes (Invitrogen)) (See Supplementary Table 4 for primer/probe sequences and efficiencies) ...
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bioRxiv - Genomics 2019Quote: ... Each reaction was performed in a 10-μl of reaction mixture simultaneously in cases and control in triplicate on a StepOnePlus Real-Time PCR System (Applied Biosystems) with SYBR Premix Ex Taq Tli RNaseH Plus (Takara Biotechnology ...
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bioRxiv - Plant Biology 2020Quote: ... followed by 40 cycles of 95°C for 15 s and 60°C for 10 min in a 7500 Real-Time PCR system (Life Technologies). Fluorescence intensities of three independent measurements (technical replicates ...
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bioRxiv - Immunology 2019Quote: ... with DNAse treatment was reverse transcribed into cDNA (Fermentas) and analyzed by real-time quantitative PCR (Fast SYBR Green;Applied Biosystems) on a 7500 Fast Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Developmental Biology 2020Quote: Relative quantity of mRNA expressed was determined by using 4ng of cDNA on a StepOnePlus Real-Time PCR System (Life Technologies). Commercially available Taqman probes from Invitrogen were utilized to implement q-PCR ...
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bioRxiv - Plant Biology 2020Quote: ... qRT-PCR was performed in 25 μL reaction volumes with gene-specific primers and conducted with a StepOne Plus Real-time PCR System (Applied Biosystems) using the SYBR Green qPCR Master Mix (Thermofisher) ...
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bioRxiv - Genetics 2019Quote: ... Genotyping of variants selected for validation in the replication cohort (described below) was performed using custom-designed TaqMan OpenArray Real-Time PCR assays (Applied Biosystems). SNPs that could not be genotyped using OpenArray assays were genotyped individually using TaqMan SNP Genotyping assays (Applied Biosystems) ...
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bioRxiv - Developmental Biology 2021Quote: ... Quantitative real-time polymerase chain reaction (RTqPCR) was then performed using Taqman gene expression assays on a QuantStudio 5 Real-Time PCR System (ThermoFisher Scientific). Gene expression was normalized to the placental reference gene Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein Zeta (YWHAZ ...
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bioRxiv - Microbiology 2020Quote: ... 40 cycles of 15 s at 95 ° C and 1 min at 60 ° C in Step-One-Plus Real-Time PCR System (Applied Biosystems). The standard curve was generated by diluting the DNA of the bacterium Escherichia coli ATCC 25922 in series of 102 – 10−8 (20 – 2 × 109 ng of DNA) ...
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bioRxiv - Physiology 2021Quote: ... Transcript levels of target genes in the equal amount of total cDNA were quantified with SYBR green chemistry (Quanta Biosciences) on a StepOnePlus Real-time PCR System (Applied Biosystems). All data were normalized to Hprt by the 2−ΔCt method ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 2019-nCoV RUO Kit (Integrated DNA Technologies) and 2019-nCoV_N positive Control (Integrated DNA Technologies) with a QuantStudio 7 Flex Real-Time PCR System (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2021Quote: ... cDNAs were quantified by qPCR using the prima-QUANT CYBR Master Mix (Steinbrenner Laborsysteme) in a QuantStudio 5 or 3 Real-Time PCR System (Applied Biosystems). The primers used are listed in Suppl ...
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bioRxiv - Neuroscience 2020Quote: ... mRNA levels were quantified by real-time PCR assay using the Quantstudio 3 System and RQ analysis software (Thermo Fisher Scientific). To ensure the specificity of the amplification ...
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bioRxiv - Microbiology 2020Quote: ... 20 ng cDNA in a 20 μL reaction and primers (all primer used for qPCR can be seen in Table S3) at 1 μM final concentration on a QuantStudio 12K Flex Real-Time PCR System (Applied Biosystems). Recorded Ct values were normalized to Ct of human HPRT1 and data plotted as ΔCt (Relative expression).
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bioRxiv - Microbiology 2020Quote: ... 40 cycles of 15 s at 95 °C and 1 min at 60 °C in Step-One-Plus Real-Time PCR System (Applied Biosystems). The proportion of bacteria in the microbiome was calculated based on the Ct (cycle threshold ...
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bioRxiv - Microbiology 2020Quote: ... A 10-ng sample of cDNA was used to perform qRT-PCR in a one-step reaction using Sybr Green master mix (Genaxxon) on a StepOne Plus real-time PCR system (Applied Biosystems). For primers used for qRT-PCR see Table S2 ...
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bioRxiv - Microbiology 2020Quote: ... followed by a final 68°C elongation step for 5 min on QuantStudio 12K Flex Real-Time PCR System (4471081, Applied Biosystems). Data were analysed using QuantStudio™ 12K Flex Software.