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Citations for Thermo Fisher :
6751 - 6800 of 10000+ citations for hsa mir 23b Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Quantitative real-time PCR was performed in triplicates using standard SYBR green reagents and protocols on a QuantStudio 5 Real-Time PCR system (Applied Biosystems). The target mRNA expression was quantified using the ΔΔCt method and normalized to ACTB (human ...
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bioRxiv - Bioengineering 2024Quote: ... RT-qPCR was performed using Firepol qPCR Master Mix (Solis BioDyne) and analyzed by 7900HT Fast Real-Time PCR System (Applied Biosystems). Fold changes were calculated using the delta Ct method ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA gene specific primers and SYBR green master mix were combined and then run in QuantStudio 5 Real-Time PCR System (Applied BioSystems). The fold-change of the genes were calculated using the ΔΔCt method using Actb as the housekeeping mRNA ...
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bioRxiv - Cell Biology 2023Quote: ... Triplicate reactions were performed using Sybr Master Mix (Life Tech-Power Sybr, 4367659) and a StepOnePlus Real-Time PCR system (Applied Biosystems).
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bioRxiv - Molecular Biology 2023Quote: qPCR was performed on MEFs using the SYBR Green Ready Master Mix in the ViiA7 Real-Time PCR System (Applied Biosystems) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: ... The mRNAs were quantified using individual TaqMan assays described in Supplemental Table 2 on an ABI QuantStudio 7 Real-Time PCR machine (Applied Biosystems) using TaqMan Fast Advanced Master Mix (Thermo Fisher ...
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bioRxiv - Molecular Biology 2022Quote: ... The reversed-transcribed RNA was used for real-time quantitative PCR (qPCR) by using Fast SYBR Green Mastermix (#4385612, Life Technologies) on a ViiA7-Real-time qPCR System from Life Technologies with the following master mix ...
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bioRxiv - Plant Biology 2023Quote: ... The PCR program comprised an initial denaturation for 30 s at 95 °C and amplification by 40 cycles of 15 s at 95 °C and 1 min at 60 °C and was ran in an ABI QuantStudio 7 Real Time PCR machine (Applied Biosystems). Expression values of DIN6 ...
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bioRxiv - Microbiology 2022Quote: ... CXR dye and primers listed in supplementary table 2 at a final concentration of 1 μM on the StepOnePlus Real-Time PCR system from Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... A volume corresponding to 5 ng of initial RNA was employed for each real-time PCR reaction using PowerUp SYBR Green Master Mix (Applied Biosystems) on a CFX Opus Real-Time PCR detection system ...
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bioRxiv - Pathology 2023Quote: ... The qPCR plates were read on a QuantStudio™ 5 Real-time PCR system (Thermo Fisher Scientific, Applied Biosystems™, MA). The reactions were done in triplicates ...
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Small Molecule Screen Identifies Pyrimethamine as an Inhibitor of NRF2-driven Esophageal HyperplasiabioRxiv - Cancer Biology 2022Quote: ... qPCR was performed to quantify the expression levels of genes of interest with relevant primers and TaqMan probes in a 96-well optical plate on an ABI 7900HT Fast Real-Time PCR system (Applied Biosystems).
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bioRxiv - Cell Biology 2023Quote: Gene expression of targets listed in supplemental table 2 were analysed by SYBR green-based qPCR analysis using Applied Biosystems QuantStudio 7 Real-Time PCR Systems (Thermo Scientific), following the standard PCR cycling sequence using GAPDH as an internal control ...
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bioRxiv - Cell Biology 2022Quote: ... Quantitative RT-PCR was performed using SYBR green Advanced Master Mix and run on a ViiA 7 Real-Time PCR System (all from Applied Biosystems). Primers were in-house designed (see table 1 ...
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bioRxiv - Molecular Biology 2023Quote: ... reactions were performed using HOT FIREPOL EvaGreen Supermix (Solis BioDyne) in a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems). Every sample was analyzed in triplicate ...
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bioRxiv - Neuroscience 2023Quote: ... RNA was reverse transcribed with qScript cDNA supermix (Quanta Bio, #95048-500) and qRT-PCR was performed with QuantaStudio 7 Real-time qPCR System (Thermofisher, #A43183). Transcript levels of Htr2c ...
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bioRxiv - Developmental Biology 2023Quote: ... and qPCR for Sfrp2 was performed using SYBR Green qPCR Reagent (Quantabio) on a ViiA 7 Real-Time PCR machine (Applied Biosystems). Primers used for qPCR are listed in Extended Data Table 2.
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bioRxiv - Neuroscience 2023Quote: ... at 1,000g for 2 min and then immediately loaded into the QuantStudio 6 and 7 Flex real-time PCR system (ThermoFisher Scientific). A two-step cycling protocol was implemented to collect cycle threshold (Ct ...
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bioRxiv - Microbiology 2023Quote: ... and 1 µL of template DNA (5-10 ng) were run on 96-well plates on a StepOne Plus Real-Time PCR system (ThermoFisher Scientific). A melting curve analysis was carried out to confirm the amplification of a single product in each reaction ...
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bioRxiv - Cancer Biology 2023Quote: ... and processed for quantitative Real-Time PCR using the Fast SYBR Green Master Mix (Applied Biosystems, Thermo Fisher Scientific, Waltham MA) and PrimePCR™ SYBR Green Assays (Bio-Rad ...
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bioRxiv - Physiology 2023Quote: ... Real-time PCR was performed using RealQ Plus 2× Master Mix Green (Ampliqon, #A324402) on a QuantStudio 5 machine (Applied Biosystems). Expression was calculated using the delta-delta-Ct method ...
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bioRxiv - Biochemistry 2023Quote: ... mNat2 mRNA expression levels were determined by quantitative polymerase chain reaction (qPCR) using the QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems). The cDNA was amplified using primers for mNat2 variant 2 and mGAPDH (see Supplementary Table S5 for details ...
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bioRxiv - Immunology 2023Quote: ... 56°C for 15 sec and 72°C for 30 sec in the Applied Biosystems 7500 or QuantStudio5 Real-Time PCR instruments (ThermoFisher Scientific). For absolute quantification ...
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bioRxiv - Plant Biology 2023Quote: ... qPCR assays were performed on an Applied Biosystems QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher Scientific, Waltham, MA, USA). The TaqMan Universal PCR master mix (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... Q-PCR was performed using HOT FIREPol EvaGreen qPCR mix Plus (ROX) (Solis Biodyne) with an ABI 7500 Real-Time PCR System (Applied Biosystems). Relative expression levels were determined by comparing the threshold cycle values for PLC2 (AT3G08510 ...
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bioRxiv - Cancer Biology 2023Quote: ... and processed for quantitative Real-Time PCR using the Fast SYBR Green Master Mix (Applied Biosystems, Thermo Fisher Scientific, Waltham MA) and PrimePCR™ SYBR Green Assays (Bio-Rad ...
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bioRxiv - Immunology 2023Quote: ... cDNA from samples was mixed with QuantiTect probe PCR master mix and gene-specific primers to run on a QuantStudio 6 Flex real-time cycler (Applied Biosystems). To generate a control for the amplification reaction ...
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bioRxiv - Immunology 2023Quote: ... and 500 nM each of the forward primer and reverse primer (Table 3) with the following cycling conditions on either QuantStudio 3 or 5 Real-Time PCR systems (Applied Biosystems): 95°C for 2 min ...
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bioRxiv - Molecular Biology 2023Quote: ... LAMP reactions were prepared as detailed in Table S2a and run at 60°C and 62°C (TSV and WSSV; respectively) for 1 hour on a thermocycler for real-time PCR (QuantStudio 12K flex, Thermo Fisher) with fluorescent measurements taken at 2-minute intervals ...
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bioRxiv - Molecular Biology 2023Quote: ... Reactions were run with 5 replicates per sample prepared as detailed in Table S2c and run at 60°C (TSV) or 62°C (WSSV) using a QuantStudio 12k Flex thermocycler for real-time PCR (Applied Biosystems) for 60 minutes ...
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bioRxiv - Plant Biology 2023Quote: ... qRT-PCR was performed using the HOT FIREPOL EvaGreen qPCR mix plus (Solis Biodyne) on a 7500 real-time PCR system (Applied Biosystems). The LjUbiquitin10 gene was used as an internal reference for analysis of the target gene expression ...
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bioRxiv - Cancer Biology 2023Quote: ... Differences in DNA content from ChIP assays were determined by qPCR using the SQ6 Real Time PCR System and SYBR Green master mix (Applied Biosystems). The reported data from at least three independent experiments represent real-time PCR values normalized to input DNA and are expressed as percentage of bound/input signal and presented as mean±SEM.
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bioRxiv - Genetics 2023Quote: ... Reactions were run in triplicate under default SYBR Green Standard cycle specifications on the Viia7 Real-Time PCR System (Applied Biosystems). Normalized relative quantification and error propagation followed the data analysis and associated calculations proposed by Taylor et al ...
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bioRxiv - Genomics 2023Quote: ... The cDNA was used as a template for real-time PCR reactions run in triplicate using pre-designed Taqman Gene Expression Assays (Life Technologies) for UCHL1 ...
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bioRxiv - Neuroscience 2023Quote: ... and SARS-CoV-2 N1 primers/probe mix (Stock# 10006713; Integrated DNA Technologies) were run on a ViiA 7 Real-Time PCR system (Applied Biosystems) as described in the instructions for use of the CDC 2019-Novel Coronavirus (2019-nCoV ...
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bioRxiv - Pathology 2023Quote: ... The qPCR plates were read on a QuantStudio™ 5 Real-time PCR system (Thermo Fisher Scientific, Applied Biosystems™, MA). The reactions were done in triplicates ...
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bioRxiv - Physiology 2023Quote: ... RNA was converted to cDNA and real-time quantitative PCR reactions were carried out using SYBR-green master mix (Applied Biosystems) on a QuantStudioTM 3 Real-time PCR instrument (Applied Biosystems) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... followed by 40 cycles of 95°C for 3seconds and 60°C for 30seconds on a StepOne Real-Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2023Quote: ... Primers specific for genes of interest were developed and real-time quantitative PCR (qPCR) reactions were carried out using SYBR-green master mix (Applied Biosystems) in a 7500 Applied Biosystems Real-time PCR machine (Applied Biosystems) ...
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bioRxiv - Neuroscience 2023Quote: ... Quantitative PCR (qPCR) was completed using the SYBR green method carried out on a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems) using LabTaq Green Hi Rox (Labtech) ...
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bioRxiv - Cancer Biology 2023Quote: ... Each sample was run in triplicates using 2-5 ng cDNA in a 384 well plate and the qPCR was performed using VIIA 7 real time PCR instrument from Applied Biosystems.
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bioRxiv - Cancer Biology 2023Quote: ... 95°C for 15 sec and ramp rate 0.075°C/sec) in a QuantStudio5 Real-Time PCR System (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2023Quote: ... Analyzes were performed using the StepOnePlus™ Real-Time PCR system with 96-well plates (Applied Biosystems, Foster City, CA, USA). From serial dilutions ...
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bioRxiv - Molecular Biology 2023Quote: ... we measured gene expression of hormonally treated endocervical cells from additional experiments with the same hormonal treatment conditions using real-time quantitative PCR (QuantStudio 12K Flex System, Life Technologies). Mean differences in relative gene expression using ANOVA were assessed with relative gene expression as the outcome and treatment as the predictor with E2 as the referent (Figure 2) ...
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bioRxiv - Cell Biology 2023Quote: ... Expression levels of mRNA were assessed by real-time PCR using the PowerUp SYBR Green Master Mix (Thermo Fisher Scientific #A25743). mRNA expression was normalized to the Hprt1 gene ...
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bioRxiv - Neuroscience 2023Quote: ... A volume corresponding to 5 ng of initial RNA was employed for each real-time PCR reaction using PowerUp SYBR Green Master Mix (Applied Biosystems) on a CFX Opus Real-Time PCR detection system ...
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bioRxiv - Plant Biology 2023Quote: ... The RT-qPCR was performed using the ChamQ Universal qPCR Master Mix (Vazyme Biotech) on the QuantStudioTM 6 Flex Real-Time PCR System (Applied Biosystems). The relative transcript level was calculated by the ΔΔCT comparative quantification method (50 ...
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bioRxiv - Cancer Biology 2023Quote: ... of gene expression was used and relative quantification was done using the equation 2-ΔΔCt (as per ‘Guide to Performing Relative Quantitation of Gene Expression Using Real-Time Quantitative PCR’ obtained from the Applied Biosystems website (http://www3.appliedbiosystems.com/cms/groups/mcb_support/documents/generaldocuments/c ms_042380.pdf).
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bioRxiv - Cancer Biology 2023Quote: ... Sensitive RT HS-PCR Mix SYBR (A&A Biotechnology, Poland) reagents were used for qPCR on One Step Plus Real-Time PCR System (Applied Biosystems) and CFX Maestro (Biorad) ...
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bioRxiv - Physiology 2023Quote: ... cDNA levels were measured by qPCR Powerup SYBRTM Green Master Mix (Ref: A25742) in a QuantStudio 5 Real-Time PCR system (Applied Biosystems, Thermo Fischer Scientific ...