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Citations for Thermo Fisher :
6451 - 6500 of 10000+ citations for hsa mir 23b Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... Complementary DNA (cDNA) was synthesized and amplified using a One Step RT PCR kit (Invitrogen) with PDLIM2-specific primers ...
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bioRxiv - Biophysics 2021Quote: ... followed by first-strand cDNA synthesis using a SuperScript™ RT-PCR kit (Invitrogen, USA) according to manufacturer instructions ...
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bioRxiv - Evolutionary Biology 2022Quote: ... using the SuperScript III One-Step RT-PCR reverse transcription kit (Thermo Fisher Scientific, Poland). Species identification was first confirmed through PCR amplification and sequencing of Cytochrome B gene (Cytb) ...
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bioRxiv - Immunology 2019Quote: ... The kits and RT-PCR amplifications were performed in ABI7500 thermocycler (Applied BioSystems®, USA). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... All 81 underwent testing through RT-PCR by TaqPath™ COVID-19 Combo Kit (ThermoFisher) under the FDA Emergency Use Authorization (EUA ...
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bioRxiv - Microbiology 2022Quote: ... Extracted RNA was assessed using the TaqPath COVID-19 CE-IVD RT-PCR Kit (Thermofisher) and cDNA was prepared using the LunaScript RT SuperMix Kit (Bioconcept) ...
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bioRxiv - Cell Biology 2023Quote: ... RT-PCR of miRNAs was performed with a Taqman microRNA Reverse Transcription Kit (Invitrogen, 4366597).
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bioRxiv - Microbiology 2023Quote: ... RT-qPCR was performed using the PowerUp SYBR Green PCR master mix kit (Applied Biosystems) in a CFX384 Touch Real-Time PCR detection system (Bio-Rad) ...
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bioRxiv - Plant Biology 2024Quote: ... RT-PCR was carried out using the High-Capacity RNA-to-cDNA Kit (Applied biosystems). Reactions for the quantitative PCR were made using 2X PowerUp SYBR Green Master Mix (Applied Biosystem ...
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bioRxiv - Cancer Biology 2024Quote: ... RT-PCR was performed using the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific). qPCR was performed using the PowerUP SYBR Green Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... using the BLOCK-iT Pol II miR RNAi expression vector kit (Invitrogen). The vector construct contained an engineered miR sequence to drive Gpr88 knock-down ...
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bioRxiv - Neuroscience 2023Quote: ... in addition to the Mir plasmids described above (Invitrogen Block it kit) were pCAGGS-GFP (gift from S ...
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bioRxiv - Immunology 2021Quote: ... ACE-2 and TMPRSS2 was measured by quantitative real time PCR (q-PCR) using the appropriate TaqMan™ assay and TaqMan™ Universal Master Mix II (Applied Biosystems, Thermo Fisher Scientific) on a ViiA™ 7 Instrument (Applied Biosystems ...
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bioRxiv - Neuroscience 2020Quote: Transcript abundance of candidate genes were assessed using StepOne Plus real-time PCR software with Fast SYBR Green PCR master mix (Applied Biosystems, Life Technologies, Carlsbad, CA, USA) using technical triplicates ...
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bioRxiv - Genetics 2019Quote: ... For real time qPCR cDNA was made using the High Capacity cDNA Reverse Transcription kit (Applied Biosystems) based on the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... at RT overnight and washed 3 times with PBS (Gibco). Chamber slides were then dried for 2h before a 1mg/mL collagen (Corning ...
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bioRxiv - Physiology 2022Quote: ... qRT-PCR reaction was performed using SYBR® Premix Ex Taq™ II (Tli RNaseH Plus) and was conducted on ABI 7500 Real-time Detection System (Thermo Fisher Scientific, USA). The PCR reaction was carried out with the following reaction conditions ...
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bioRxiv - Microbiology 2020Quote: ... in 20 μl reactions using AgPath-ID One-Step RT-PCR Reagents 10 μl RT-PCR buffer (2X) (Thermo Fisher), 5μl of RNA ...
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bioRxiv - Microbiology 2020Quote: ... One-step RT-PCR was performed using the Super-Script III One-Step RT-PCR System with Platinum Taq (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... The co-transcription was analyzed by RT-PCR using SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase (Invitrogen), following the manufacturer’s instructions.
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Genomic and phylogenetic analyses of SARS-CoV-2 strains isolated in the city of Gwangju, South KoreabioRxiv - Microbiology 2020Quote: ... 1 μL of 25X RT-PCR enzyme mixture included in AgPath-ID One-step RT-PCR Reagents (Thermo Fisher Scientific), 5 μL of RNA ...
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bioRxiv - Microbiology 2021Quote: RT-PCRs were performed using a SuperScript III One-Step RT-PCR with Platinum Taq DNA Polymerase system (Thermo Fisher). Cycling conditions were as follows ...
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bioRxiv - Microbiology 2021Quote: RT-PCRs were performed using the SuperScript III One-Step RT-PCR with Platinum Taq DNA Polymerase system (Thermo Fisher). Cycling conditions were as follows ...
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bioRxiv - Microbiology 2021Quote: ... The RT-PCR was performed using either the SuperScript III Platinum One-Step Quantitative RT-PCR system (Invitrogen, Carlsbad, CA) or Taqman Fast Virus 1-step master mix (Applied Biosystems ...
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bioRxiv - Immunology 2019Quote: ... Gene expression was quantified by quantitative RT-PCR using a StepOnePlus RT-PCR machine (Applied Biosystems, Foster City, CA, USA), TaqMan Fast Advanced Master Mix (Applied Biosystems ...
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bioRxiv - Microbiology 2022Quote: ... RT-PCR was performed in a 96-well plate using an Applied Biosystems QuantStudio 6 RT-PCR system (ThermoFisher Scientific). Reactions of 50 μLs in total volume were used which contained ...
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bioRxiv - Microbiology 2023Quote: ... A total of 8.5 µL extracted RNA was subjected to one-step RT-PCR using the Superscript III one-step RT-PCR system (Invitrogen). To amplify the pol region ...
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bioRxiv - Molecular Biology 2023Quote: ... the TFAM mRNA was amplified by RT-PCR using the SuperScript IV One-Step RT-PCR System (Thermo Fisher Scientific). The RT-PCR reaction was further amplified by PCR using inner primers (Extended Table 1).
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bioRxiv - Microbiology 2023Quote: ... A total of 8.5 µL extracted RNA was subjected to one-step RT-PCR using the Superscript III one-step RT-PCR system (Invitrogen). The one-step RT-PCR was performed using the forward primer Pol F1 5’-TACAGTGCAGGGGAAAGAATA-3’ (nt 4809-4829 ...
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bioRxiv - Synthetic Biology 2023Quote: ... DNA removal from RNA fractions was confirmed by RT-PCR using the SuperScript IV One-Step RT-PCR System (Invitrogen) with and without addition of SuperScript IV RT Mix ...
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bioRxiv - Molecular Biology 2023Quote: ... or used directly as a template for RT-PCR using SuperScript IV One-Step RT-PCR System (Cat# 12594100, Invitrogen).
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bioRxiv - Bioengineering 2023Quote: ... PCR employed the TaqMan Universal PCR Master Mix II with miR-specific Small RNA Assays (Thermo Fisher Scientific, Waltham, MA, USA), and proceeded according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: Total miR levels in extracted RNA samples were quantified using a Qubit miR Assay Kit and Qubit 3.0 Fluorimeter (Thermo Fisher Scientific, Waltham, MA, USA); the manufacturer’s protocols were employed.
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bioRxiv - Immunology 2024Quote: ... RT-qPCR was used to detect and determine virus titers using the AgPath ID one-step RT-PCR kit (ThermoFisher Scientific, Waltham, MA) from the cell culture supernatants as previously described [28] ...
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bioRxiv - Cancer Biology 2019Quote: ... precursor and mature miR-15a/miR-16 specific primers were purchased from Thermo Fisher. cDNA synthesis was performed by the High Capacity cDNA Synthesis Kit (Thermo Fisher ...
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bioRxiv - Cancer Biology 2022Quote: ... and pre-miR miRNA mimics or pre-miR control 1 (all ThermoFisher, Leicestershire, UK). Luciferase activity was quantified at 24 hrs using the dual-glo luciferase assay system (Promega ...
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bioRxiv - Microbiology 2023Quote: ... miR-19a (Assay ID: 000395) and miR-92a (Assay ID:000430) (Applied Biosystems, USA), as per manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... miR-16 mimic (miR-16; 50 nM; mirVana® miRNA mimic; MC10339; ThermoFisher Scientific), or the respective negative controls [Lincode Non-targeting Pool (CTR lncRNA ...
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bioRxiv - Microbiology 2019Quote: ... Reverse transcription (RT) was performed with the ThermoScript™ RT-PCR System (ThermoFisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... RT-PCR was performed on ProFlex™ PCR (Thermo Fisher Scientific, USA) using DNA polymerase (QingkeBiotech ...
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bioRxiv - Cell Biology 2020Quote: ... and quantitative RT-PCR were from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Genetics 2021Quote: ... and RT-PCR Grade Water (Thermo Fisher Scientific) were used as positive and negative controls for each batch of genotyping ...
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bioRxiv - Molecular Biology 2021Quote: ... in a StepOnePlus RT-PCR System (Thermo Fisher).
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bioRxiv - Immunology 2019Quote: ... on a StepOne RT-PCR System (Applied Biosystems). Expression levels were normalized to Gapdh or 18S using the ΔCt method or ΔΔCt relative to control groups.
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bioRxiv - Developmental Biology 2019Quote: ... RT-PCR products were cloned (TOPO-TA; Invitrogen), and the plasmids purified from individual colonies was/were Sanger-sequenced according to standard protocols to identify the precise alteration of the endogenous gclc transcript.
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bioRxiv - Genetics 2020Quote: ... and RT-PCR Grade Water (Thermo Fisher Scientific) were used as the positive and negative controls ...
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bioRxiv - Evolutionary Biology 2020Quote: ... A one-step hemi-nested RT-PCR (Invitrogen) was used to detect coronavirus RNA using a set of primers targeting a 440-nt fragment of the RdRp gene and optimized for bat-CoV detection (CoV-FWD3 ...
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bioRxiv - Cancer Biology 2020Quote: ... using a StepOnePlus RT-PCR system (Applied Biosystems). Reactions were run in triplicate and mouse Actb was used as a housekeeping gene ...
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bioRxiv - Physiology 2020Quote: ... in a RT-PCR System (StepOnePlus, Applied Biosystems). PCR reactions were done in duplicate with the addition of negative controls (i.e. ...
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bioRxiv - Physiology 2019Quote: ... Taqman RT-PCR reagents (Applied Biosystems, Carlsbad, CA) were used to generate all cDNA ...