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Citations for Thermo Fisher :
6401 - 6450 of 10000+ citations for hsa mir 23b Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... The reactions were run on the QuantStudio™ 5 Real-Time PCR System in 384-well plates (Thermo Fisher Scientific). All expression assays were normalised to the geometric mean of the reference genes GAPDH/Gapdh and ACTB/Actb.
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bioRxiv - Genomics 2023Quote: ... then 36 cycles of 20s at 94°C and 60s at 54°C performed on an ABI9700 thermocycler followed by a final point read of the fluorescence on an ABI QuantStudio 6 real-time PCR system and using the QuantStudio software 1.3 (Applied Biosystems). For the genotyping of crude biological samples (whole blood or neutralized NaOH treatment solution of ear biopsies) ...
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bioRxiv - Genomics 2023Quote: ... then 36 cycles of 20 s at 94°C and 60 s at 54°C performed on an ABI9700 thermocycler followed by a final point read of the fluorescence on an ABI QuantStudio 6 real-time PCR system and using the QuantStudio software 1.3 (Applied Biosystems).
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bioRxiv - Biochemistry 2023Quote: ... The fluorescence intensity was measured as a continuous function of temperature using a Veriti real-time PCR device (Applied Biosystems) to monitor protein unfolding ...
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bioRxiv - Developmental Biology 2023Quote: ... and Ccnd1 was performed using SYBR Green qPCR Reagent (Quantabio) on a ViiA 7 Real-Time PCR machine (Applied Biosystems), as described above ...
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bioRxiv - Bioengineering 2023Quote: ... All amplifications were performed under the same thermal cycling conditions using a QuantStudio 3 real-time PCR system (Applied Biosystems). Initial denaturation was performed at 95°C for 1 min ...
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bioRxiv - Cell Biology 2022Quote: ... TNFα and ICAM1 gene expression levels were determined using SYBR green-based quantitative real-time PCR (Applied Biosystems, Darmstadt, Germany) using the 7900GR Fast Real-time PCR system (Applied Biosystems) ...
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bioRxiv - Genetics 2023Quote: ... Vector titers in vg/mL were determined by qPCR using an ABI Fast 7500 Real-time PCR system (Applied Biosystems), with Applied Biosystems TaqMan Fast Universal PCR Master Mix 2x ...
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bioRxiv - Developmental Biology 2023Quote: qPCR was performed with a Quantstudio-3 Real-Time PCR System and PowerUp SYBR Green Master Mix (Thermo Fisher Scientific). Two independent biological replicates were performed with three technical replicates each ...
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Apical expansion of calvarial osteoblasts and suture patency is dependent on graded fibronectin cuesbioRxiv - Developmental Biology 2023Quote: ... Relative mRNA expression was quantified using 4 ng of cDNA on a StepOne Plus Real-Time PCR System (Life Technologies) and the ΔΔCT method (Schmittgen & Livak ...
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bioRxiv - Physiology 2023Quote: ... The concentrations of the RNA libraries were measured using a StepOnePlus Real-Time PCR System (ThermoFisher Scientific, San Jose, CA) with the KAPA Library Quantification Kit (Kapabiosystems ...
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bioRxiv - Bioengineering 2023Quote: ... and fungal (18SF) communities in the anode biofilm were determined using a QuantStudio 3 Real-Time PCR system (ThermoFisher, USA) and the 16S rRNA and 18S rRNA genes as molecular markers ...
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bioRxiv - Genetics 2023Quote: RT-PCR analyses of miRNA (TaqMan) levels were performed according to manufacturer’s instructions with the StepOnePlus and QuantStudio 3 Real-Time PCR Systems (Applied Biosystems). Levels were normalized to U18 snoRNA ...
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bioRxiv - Bioengineering 2023Quote: ... The protein samples were heated from 25 °C to 95 °C at a rate of 0.3°C/sec in a StepOnePlus real-time PCR system (Applied Biosystems), and fluorescence was captured in the ROX channel ...
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bioRxiv - Plant Biology 2023Quote: ... All the primers for the real-time PCR analysis were designed using the ‘Primer Express 2.0’ software (expected product size = 100–120 bp) (Life Technologies) followed by the individual pair confirmation using the BLAST program in the RGAP database (rice.plantbiology.msu.edu ...
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bioRxiv - Microbiology 2023Quote: ... KiCqStart SYBR qPCR Ready Mix was used to perform qPCR using the Fast Dx Real-Time PCR Instrument (Applied Biosystems). Primers specific to the 5′ untranslated region (5′ TAACCCGTGTGTAGCTTGG-3′ and 5′ - ATTAGCCGCATTCAGGGGC-3′ ...
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bioRxiv - Physiology 2023Quote: ... qPCR was performed using the QuantStudio™ 7 Flex Real Time PCR System (Applied Biosystems™, New York, NY, USA) and SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Genetics 2024Quote: Quantitative real-time PCR was performed in 10 μl reactions using PowerTrack™ SYBR Green Master Mix (Applied Biosystems A46109), 1:10 diluted cDNA ...
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bioRxiv - Neuroscience 2024Quote: ... Real-time qRT-PCR was performed on the QuantStudio 6 Flex thermocycler using PowerUp SYBR green master mix (ThermoFisher Scientific). All reactions were prepared according to the manufacturer instructions ...
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bioRxiv - Physiology 2024Quote: ... characterised LRRK2 (Hs01115057_m1) and LYZ (Hs00968202_m1) gene expression normalised to B2M (Hs00187842_m1) on a StepOnePlus™ real-time PCR system (Applied Biosystems, Thermo Fisher Scientific UK Ltd. ...
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bioRxiv - Cancer Biology 2023Quote: ... and the Applied Biosystems® ViiATM 7 Real-Time PCR System with 384-well Block (Thermo Fisher Scientific; Cat #4453536). We used the comparative Ct (ýýCt ...
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bioRxiv - Cancer Biology 2023Quote: ... following manufacturer’s protocols and gene expression quantified via Applied Biosystems Quant Studio 6/7 Real-Time PCR System (Applied Biosystems), for TaqMan (Thermo Fisher Scientific ...
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A gene desert required for regulatory control of pleiotropic Shox2 expression and embryonic survivalbioRxiv - Developmental Biology 2023Quote: ... and on a and on a ViiA 7 Real-Time PCR System using PowerTrack SYBR Green Master Mix (Applied Biosystems) for SV-EnhΔ samples ...
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bioRxiv - Microbiology 2023Quote: ... Reactions were assembled following the manufacturer’s instructions and were performed in a 7500 Real-Time PCR System (Applied Biosystems, USA).
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bioRxiv - Cell Biology 2023Quote: ... 40 cycles of 95°C for 1 s and 60°C for 20 s on the QuantStudio 5 Real-Time PCR System (ThermoFisher). Relative quantification of each gene was done by QuantStudio Design&Analysis v1.5.1 software (ThermoFisher ...
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bioRxiv - Microbiology 2023Quote: ... The qPCR assays were conducted in triplicate with an ABI 7500 Real-Time PCR System (Applied Biosystems, Waltham, MA, USA). For plant DNA ...
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bioRxiv - Immunology 2023Quote: ... SFB736F (5′-GACGCTGAGGCATGAGAGCAT-3′)/SFB844R (5′-GACGGCACGGATTGTTATTCA-3′) were used in a 7500 Fast Real-Time PCR System (Life Technologies) to quantify SFB level in the feces ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 0.2 and 0.04 ng/µl to generate standard curves with StepOnePlus Real-Time PCR System v2.2 (ABI, Thermo Fisher Scientific). Primer quality was 125% efficiency ...
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bioRxiv - Cancer Biology 2023Quote: ... qPCR plates were covered with a clear plastic adhesive and placed into QuantStudio Dx Real-Time PCR instruments (ThermoFisher Scientific) run using the following amplification conditions ...
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bioRxiv - Microbiology 2024Quote: ... Incubation mixtures were arranged in 96-well plates and introduced into the StepOne Real-Time PCR equipment (Thermo Fisher Scientific). Samples were heated from 10 °C to 80 °C and fluorescence was measured at each point with SYBR Green filters ...
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bioRxiv - Molecular Biology 2024Quote: ... The diluted pre-amplified products were then utilized directly for real-time PCR reactions according to the manufacturer’s protocol (Applied Biosystems™ TaqMan™ Array Human MicroRNA A+B Cards Set v3.0 ...
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bioRxiv - Physiology 2024Quote: ... Semi-quantitative real-time PCR analysis was conducted using PowerUp™ SYBR™ Green master mix (Thermo Scientific™ #A25741). Relative expression levels for each gene of interest were calculated with the ΔΔCt method ...
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bioRxiv - Immunology 2024Quote: ... RT-qPCR reactions were assayed in triplicates per sample using a QuantStudioTM 5 Real-Time PCR system and TaqMan Gene expression assays (all Thermofisher): Lyve-1 (Mm00475056_m1) ...
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bioRxiv - Cancer Biology 2024Quote: ... Gene expression levels were measured by quantitative real-time PCR using the SYBR Green assay (Thermo Scientific, Rockford, IL, USA)on a StepOne instrument (Thermo Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... Thermal cycling and data analysis were performed using the StepOne Plus Real-Time PCR System and StepOne Software (Applied Biosystems) to detect quantitative mRNA expression of Cathepsin-K and DC-STAMP (primer sequences are listed in supplementary Table S3) ...
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bioRxiv - Molecular Biology 2024Quote: ... The resulting cDNA was used for real-time PCR analysis using a Platinum SYBR Green qPCR SuperMix (Thermo Fisher Scientific). Next ...
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bioRxiv - Plant Biology 2024Quote: ... Quantitative PCR was carried out in 96-well plates using gene specific primers and a VWR qPCR Master Mix (Avantor) for 40 cycles on a QuantStudio 6 Flex Real-Time PCR System (ThermoFisher).
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bioRxiv - Plant Biology 2024Quote: ... with low ROX reference dye according to the manufacturer’s instruction and run on a QuantStudio 5 Real-Time PCR System (Thermo Fisher). Each reaction was performed in 2-3 technical ...
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bioRxiv - Neuroscience 2024Quote: ... with real time quantitative gene expression determined using using Taqman probles (Table 2) and analysed by the Quant Studio 6 Flex Real-Time PCR System (Invitrogen).
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bioRxiv - Neuroscience 2024Quote: ... qPCR was performed by using 2×qPCR SmArt Mix (DEEYEE, Shanghai, China) with StepOnePlus Real-time PCR system (Thermo Fisher). The fold-change data were obtained using the delta-delta Ct method (Livak and Schmittgen ...
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bioRxiv - Immunology 2024Quote: ... All RT-qPCR reactions were carried out using a 7500 Real-Time PCR System and data were analyzed using the 7500 Software version 2.3 (Applied Biosystems). RT-qPCR data are reported as the LRP1 transcript abundance in each sample compared to the GAPDH housekeeping gene as calculated by the comparative cycle threshold method (2(- ΔCt)) ...
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bioRxiv - Synthetic Biology 2024Quote: ... The reaction was performed in MicroAmp® Fast Optical 96-Well Reaction Plate with Barcode 0.1 ml (Applied Biosciences 4346906) and run in 7500 Real-Time PCR system (ThermoFisher Scientific). Primers with efficiency (>90% ...
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bioRxiv - Microbiology 2024Quote: ... 56°C for 15 s and 72°C for 30 s in the Applied Biosystems 7,500 or QuantStudio5 Real-Time PCR instruments (ThermoFisher Scientific). For absolute quantification ...
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bioRxiv - Neuroscience 2020Quote: ... and miR-9/9*-124 + TuD-miR-124 using TRIzol Reagent (Invitrogen, 15596026) in combination with RNeasy micro kit (Qiagen ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Two different miR-146a mimics (Ambion/Thermo Pre-miR™ and miRVana mimic) were used ...
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bioRxiv - Microbiology 2023Quote: ... Quantitative real-time polymerase chain reaction (qPCR) analysis was performed using the Applied Biosystems QuantStudio™ 6 Flex Real-Time PCR System (Thermo Fisher Scientific Inc., Waltham, MA, USA). Analysis of endosymbiont DNA was performed using the SYBR Green PCR Master Mix (Life Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... hippocampal neurons were transfected at DIV7 with 10nM of the miR-499-5p or control mimic (Ambion™ Pre-miR miRNA Precursor: miR-499-5p and Neg Control 1, Thermo Fisher) with the Lipofectamine RNAiMAX reagent (Thermo Fisher ...
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bioRxiv - Plant Biology 2020Quote: ... the ViN vectors and housekeeping genes was quantified using qRT-PCR performed with the one-step SuperScript RT-PCR kit (Invitrogen) on a BioRAD thermocycler ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... A partial cDNA encoding AHR was amplified by RT-PCR using the GeneAmp Gold RNA PCR Reagent Kit (Applied Biosystems). Total RNA (1 µg per reaction ...
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bioRxiv - Immunology 2021Quote: ... cDNA was obtained using Verso cDNA kit Reverse Transcription for RT-PCR (Thermo Fisher Scientific), following the manufacturer’s instructions ...