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Citations for Thermo Fisher :
6801 - 6850 of 10000+ citations for hsa mir 23b Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... stock was added to each sample directly before measurement of the melting temperature in a 96-well plate on a QuantStudio 1 Real-Time PCR System reader (Thermo Fisher) with a temperature increase of 0.05 °C/min ...
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bioRxiv - Neuroscience 2023Quote: ... The quantification of gene expression levels was performed by quantitative Real-time PCR using PowerUp SYBR Green Master Mix (Applied biosystems) and QuantaStudio5 instrument (Applied biosystems) ...
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bioRxiv - Plant Biology 2024Quote: ... The obtained cDNA was quantified by qPCR using SG qPCR Master Mix (2×) plus ROX Solution (EURx, Gdansk, Poland) and a StepOnePlus Real-Time PCR System (Applied Biosystems). The cDNA was diluted 10-fold and 10 μl was used in a total reaction volume of 25 μl per well ...
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bioRxiv - Physiology 2024Quote: ... prior to qPCR-based measurement of mRNA with SYBR green chemistry on a Quant-Studio 6-flex real-time PCR system (ThermoFisher Scientific). All data were normalized to Hprt1 or Actb by the 2-delta Ct method (100) ...
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bioRxiv - Microbiology 2023Quote: ... Thermocycling for qPCR and subsequent melt analysis were performed on a Quantstudio 3D real time PCR system (Applied Biosystems, Waltham, MA). The cycling conditions were as follows ...
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bioRxiv - Microbiology 2023Quote: ... The prepared cDNA (0.25 µl) were used for quantitative real time-PCR using 2x sybr green master mix (cat no.4344463, applied biosystems/Thermofisher scientific) to measure the amount of CHPV-N specific mRNA in infected Vero cells using CHPV-N specific primers (forward 5′-ACCTGGCTCCAAATCCAATAC-3′ and reverse 5′-GGTGGATCAGACGGAGAGATA-3′) ...
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bioRxiv - Cell Biology 2023Quote: ... The qPCR experiments were conducted on the StepOnePlus Real-Time PCR System running the StepOne Design and Analysis Software (Applied Biosystems). All reaction conditions and protocols were performed according to the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2023Quote: ... The qPCR was initiated by 10 min at 95 °C followed by 40 thermal cycles of 15 s at 95 °C and 1 min at 60 °C in a StepOnePlus real-time PCR system (Applied Biosystems). Table 1 lists the sequences of the primers used.
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bioRxiv - Cell Biology 2023Quote: ... Same cDNA was used to amplify both the gene of interest and 18S rRNA in Quant-Studio 6 Flex real-time PCR system (Applied Biosystems). The PCR conditions consisted of AmpliTaq Gold activation at 95°C for 10 min ...
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bioRxiv - Genetics 2023Quote: ... qRT-PCR for ERG11 and CDR1/PDR5 together with ACT1/TDH3 as housekeeping gene were amplified from the synthesized cDNA using primers listed in Table S2 with a QuantStudio 3 real-time PCR system using the ΔΔCT method (ThermoFisher Scientific).
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bioRxiv - Biochemistry 2023Quote: ... A temperature ramp from 5°C to 95°C was set up at a speed of 0.02°C/s on a QuantStudio 3 Real-Time PCR system (Thermo Fisher Scientific). All measurements were performed in triplicates ...
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bioRxiv - Plant Biology 2023Quote: ... The RT-qPCR was performed using the ChamQ Universal qPCR Master Mix (Vazyme Biotech) on the QuantStudioTM 6 Flex Real-Time PCR System (Applied Biosystems). The relative transcript level was calculated by the ΔΔCT comparative quantification method (50 ...
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bioRxiv - Bioengineering 2024Quote: ... RT-qPCR was performed using Firepol qPCR Master Mix (Solis BioDyne) and analyzed by 7900HT Fast Real-Time PCR System (Applied Biosystems). Fold changes were calculated using the delta Ct method ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA gene specific primers and SYBR green master mix were combined and then run in QuantStudio 5 Real-Time PCR System (Applied BioSystems). The fold-change of the genes were calculated using the ΔΔCt method using Actb as the housekeeping mRNA ...
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bioRxiv - Pathology 2023Quote: ... qPCR was performed using TaqMan universal master mix II and RNA primers (20x) in 10 μL reactions on a StepOnePlus Real-Time PCR Systems (ThermoFisher Scientific). The results were normalized to the 18S level in each sample ...
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bioRxiv - Neuroscience 2023Quote: ... The qPCR plates were read on a QuantStudio™ 5 Real-time PCR system (Thermo Fisher Scientific, Applied Biosystems™, MA). The reactions were done in triplicates ...
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bioRxiv - Plant Biology 2024Quote: ... 3 μl of the dilution series and cocktail mix were dispensed into duplicate wells on a 384-well ABI optical plate and the plate was loaded on the ABI 7900HT Fast Real-Time PCR System (Applied Biosystems). The FAM-Non Fluorescent Quencher detector and Rox passive reference were selected ...
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bioRxiv - Microbiology 2024Quote: ... Library concentrations were then normalized to 4nM and validated by qPCR on a QuantStudio-3 real-time PCR system (Applied Biosystems), using the Kapa library quantification kit for Illumina platforms (Cat ...
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bioRxiv - Physiology 2024Quote: ... Quantitative Real-Time PCR was conducted using Taq Man Gene Expression Master Mix (Applied Biosystems-Thermo Scientific-Vilnius, Lithuania: catalog #4444557) in the Rotor-Gene Q real-time PCR cycler machine (Qiagen ...
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bioRxiv - Cell Biology 2024Quote: ... The data were collected from at least three independent biological replicates using StepOnePlus or QuantStudio 3 Real-Time PCR Systems (ThermoFisher Scientific).
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bioRxiv - Evolutionary Biology 2024Quote: ... The samples were amplified in a single run on the QuantStudio 3 Real-Time PCR System (Thermo Fisher, Cat. No. A28567).
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bioRxiv - Genetics 2024Quote: ... Samples were then analyzed using qPCR with TaqMan Fast Advanced Master Mix primers and probes using 7500 Real-Time PCR System (Applied Biosystems). The cycling conditions were 50C for 2min ...
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bioRxiv - Genetics 2024Quote: ... the cDNA products were subjected to real-time PCR with Fast SYBR Green Master Mix (QuantStudio 12K Flex system, Applied Biosystems) to determine the expression level of firefly luciferase relative to that of Renilla luciferase.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Quantitative PCR was done in Optical 96- or 384-well plates (Applied Biosystem) using the 7300 or 7900 Real Time PCR System (Applied Biosystems) and GoTaq qPCR Master Mix (Promega) ...
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Aberrant regulation of serine metabolism drives extracellular vesicle release and cancer progressionbioRxiv - Cancer Biology 2024Quote: ... The expression levels of mature miRNAs were measured using real[time qPCR with TaqMan Universal PCR Master Mix (4324018, Applied Biosystems). The data were collected and analyzed using StepOnePlus (Applied Biosciences) ...
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bioRxiv - Cancer Biology 2024Quote: ... Gene expression was analyzed by qPCR using Power SYBR green fast master mix and run on a 7500 Fast Real-Time PCR system by Applied Biosystems. Primer sequences used in the experiments are tabulated below.
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bioRxiv - Cell Biology 2024Quote: ... Triplicate reactions for each cDNA and primer pair were run on a QuantStudio 5 Real-Time PCR System (Thermo Fisher Scientific) and transcript levels relative to GAPDH were calculated using the ΔCt method.
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bioRxiv - Cancer Biology 2024Quote: ... and KiCqStart pre-designed SYBR green gene-specific primers (Supplementary Table S4) in QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems). Expression level for each gene was normalized to GAPDH for each experiment ...
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bioRxiv - Biophysics 2024Quote: ... Expression was quantified using the 2-ΔΔCt method and amplification was carried out using an Applied Biosystems 7500 Real Time PCR system (Thermo Fisher).
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bioRxiv - Microbiology 2024Quote: ... Primers and probes for quantitative real-time PCR were generated with Primer Express equipment (version 1.0, Applied Biosystems, Foster City, CA) to amplify BoHV-1 glycoprotein B sequences with an open reading frame (ORF) ...
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bioRxiv - Neuroscience 2024Quote: ... The resulting cDNAs were diluted to 4 ng/µL and used as templates in real-time quantitative polymerase chain reactions (PCRs; QuantStudio3; Applied Biosystems). TaqMan oligonucleotide primers and probe sets (TaqMan Gene Expression Assays ...
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bioRxiv - Biochemistry 2024Quote: ... using a QuantStudio 3 Applied Biosystems real-time polymerase chain reaction (PCR) system with differential scanning fluorimetry capability (Thermo Fisher Scientific). Assays were repeated in triplicate in a 96-well 0.2-mL PCR-compatible plates ...
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bioRxiv - Immunology 2024Quote: ... TaqMan genotyping assays were carried out according to the standard protocol on an ABI 7500 Real-Time PCR System machine using Genotyping Master Mix (Applied Biosystems).
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bioRxiv - Neuroscience 2024Quote: ... Triplicate 20 μL samples were subjected to analysis on a StepOne-PlusTM Real-Time PCR system (Applied BiosystemsTM, Thermo Fisher Scientific). Each sample included nuclease-free H2O (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... Product purity was validated through a melt curve analysis using ABI hardware and software (QuantStudio Real-Time PCR Systems, Thermo Scientific), and gene expression analyses were determined using the comparative ΔΔCt (fold change ...
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bioRxiv - Immunology 2024Quote: ... We quantified target mRNA and viral RNA levels using real-time qPCR assay with specific fluorogenic probe-primer combinations and Fast Universal PCR Master Mix systems (Applied Biosystems) with mouse-specific forward and reverse primers and probes as described previously (36 ...
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bioRxiv - Immunology 2024Quote: ... The reactions were set up using the manufacturer’s guidelines and run on a QS5 Studio Real-Time PCR instrument (Thermo Fisher Scientific). Values are represented as the difference in cycle threshold (Ct ...
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bioRxiv - Immunology 2024Quote: ... All samples were assayed using the 7300HT or QuantStudio 6 Fast Real-Time PCR System and analyzed using Fast System Software (Applied Biosystems). All real-time PCR data was normalized to the level of Gapdh mRNA ...
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bioRxiv - Microbiology 2024Quote: ... The relative level of transcript of interest was quantified using the comparative ΔΔCt method using the hrdB transcript as the endogenous control (StepOne Plus real-time PCR system, Applied Biosystems).
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bioRxiv - Microbiology 2024Quote: ... isolated RNA was run using a TaqMan Fast Virus One-Step Master Mix (applied Biosystems) on a QuantStudio 3 Flex Real-Time PCR system (Applied Biosystems). RNA standards with known copy numbers were used to generate a standard curve and calculate sample copy numbers ...
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bioRxiv - Neuroscience 2024Quote: ... with custom IDT RT-qPCR assays (Sup Methods Table) and TaqMan assays (GAPDH-Jun assay 4485713) on a QuantStudio 5 Real-Time PCR system (Applied Biosystems) and quantified using the ΔΔCt method35 ...
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bioRxiv - Cell Biology 2021Quote: ... miR-30a-3p (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... the miR designer by Thermofisher was used ...
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bioRxiv - Evolutionary Biology 2020Quote: ... quantitative RT-PCR (qRT-PCR) was done using Power SYBR PCR master mix (Thermo Fisher) and in technical triplicates each time.
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bioRxiv - Cancer Biology 2022Quote: ... Quantitative RT-PCR (qRT-PCR) was performed using TaqMan Universal PCR Master Mix (Applied Biosystems) and TaqMan gene expression assays (Life Technologies ...
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bioRxiv - Molecular Biology 2020Quote: ... Real-time reactions were assembled using Taqman probes (Applied Biosystems) in accordance with the manufacturer’s directions ...
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bioRxiv - Molecular Biology 2019Quote: ... on a ViiA-7 real-time thermal cycler (Applied Biosystems). All samples were analysed in technical duplicates ...
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bioRxiv - Microbiology 2021Quote: Real time qPCR was performed with an ABI7300 (Applied Biosystems). Quantitation of sense or antisense viral N transcripts was performed by a Taqman probe assay ...
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bioRxiv - Neuroscience 2021Quote: ... on a StepOnePlus™ Real-Time qPCR System (Applied Biosystems).
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bioRxiv - Microbiology 2019Quote: ... in an ABI Prism 7300 real-time system (Applied Biosystems). 50 ng of cDNA were used in each reaction ...