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Citations for Thermo Fisher :
6501 - 6550 of 10000+ citations for hsa mir 23b Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... and 2.375 µl RT-PCR grade water (Invitrogen). PCR products were cleaned up with 0.8:1 Ampure XP beads (Beckman Coulter ...
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bioRxiv - Neuroscience 2020Quote: ... on a StepOnePlus RT PCR system (Applied Biosystems) or a LightCycler 96 (Roche) ...
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bioRxiv - Microbiology 2021Quote: ... The SuperScript IV RT-PCR system (ThermoFisher Scientific) was used for generating cDNA according to manufacturer’s procedures and final PCR reactions were performed according to standard protocols.
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bioRxiv - Immunology 2022Quote: ... RT-PCR was performed with StepOnePlus (Applied Biosystems) using SYBR Green PCR Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... Using a QuantStudio 3 RT-PCR thermocycler (Invitrogen), the DSF samples were incubated at 5°C for 1 min ...
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bioRxiv - Immunology 2024Quote: ... on a QuantStudio 5 RT-PCR (Applied Biosystems). Relative gene expression values were calculated using the delta-delta Ct method ...
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bioRxiv - Genomics 2022Quote: SuperScript IV One-Step RT-PCR System (Invitrogen) was used for RSV whole genome amplification with two separate multiplex RT-PCR reactions using six pairs of primers designed for this study ...
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bioRxiv - Neuroscience 2023Quote: ... RT-PCR TaqMan gene expression assays (Applied Biosystems) were used to measure the expression of β-actin (ACTB) ...
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bioRxiv - Developmental Biology 2023Quote: ... and a StepOnePlus RT PCR system (Applied Biosystems). Relative gene expression levels were normalized to GAPDH ...
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bioRxiv - Cell Biology 2024Quote: ... RT-PCR was performed using StepOnePlus System (ThermoFisher) with qPCRBIO SyGreen® Mix Hi-ROX (PCR Biosystems ...
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bioRxiv - Molecular Biology 2024Quote: ... RT-PCR was performed with QuantStudio (Applied Biosystems) and SYBR Premix Ex Taq II (Takara Bio ...
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bioRxiv - Microbiology 2020Quote: ... that includes a reference curve of viral RNA at known copy number with a 7500 Fast Real-Time PCR System (Applied Biosystems).
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bioRxiv - Neuroscience 2021Quote: ... Gene expression was determined by qPCR using Brightgreen Dye qPCR master mix on a ViiA 7 Real Time PCR System (Applied Biosystems). Primer sequences were designed using NCBI Primer Blast ...
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bioRxiv - Biophysics 2021Quote: ... 0.25 µl of forward primer and 0.25 µl of reverse primer were added to each probe well and the qPCR was performed in a QuantStudio™ 3 Real-Time PCR system (ThermoFisher). Data were normalized to the housekeeping gene GAPDH ...
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bioRxiv - Cancer Biology 2021Quote: ... ChIP-qPCR was performed using the SYBR Green qPCR Master Mix (SolomonBio, QST-100) on the QuantStudio 3 Real-Time PCR system (Applied Biosystems). Primers were listed in Supplementary Table S1.
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bioRxiv - Microbiology 2020Quote: ... cDNAs were subjected to qPCR using KOD SYBR qPCR Mix (TOYOBO) in the StepOnePlus Real-time PCR system (Thermo Fisher Scientific) (1 µL of cDNA per 10 µL reaction) ...
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bioRxiv - Immunology 2021Quote: ... Quantitative real-time PCR was performed using PowerUp SYBR Green Gene Expression Assays (Thermofischer A25741) and a QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems). Relative expression values were calculated using the comparative Ct (ΔΔCt ...
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bioRxiv - Developmental Biology 2021Quote: ... and gene primers (Supplementary table 1) were used to perform qPCR on QuantStudio 6 Flex Real-Time PCR system (Thermo Fisher) and normalized to housekeeping gene Tbp ...
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bioRxiv - Genetics 2021Quote: ... RT-qPCR was performed using Firepol qPCR Master Mix (Solis BioDyne) and analyzed by 7900HT Fast Real-Time PCR System (Applied Biosystems). Fold changes were calculated using the delta Ct method ...
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bioRxiv - Microbiology 2021Quote: ... burgdorferi co-cultures transcript experiments were performed using PerfeCTa SYBR Green FastMix ROX (Quantabio) and StepOnePlus Real-Time PCR system (Applied Biosystems). flaB was used as an internal control and fold change relative to wild type (WT ...
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bioRxiv - Microbiology 2021Quote: ... Quantitative real-time PCR was carried out using ChamQ Universal SYBR qPCR Master Mix (Vazyme) and specific primers on the 7500 Fast Real-Time PCR Instrument (Applied Biosystems). Data were analyzed using GraphPad Prism Version 8.0 (GraphPad Software ...
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bioRxiv - Developmental Biology 2020Quote: ... Relative mRNA expression was measured with SsoAdvanced Universal Probes Supermix reactions read out with a QuantStudio 3 real-time PCR system (Applied Biosystems). TATA-box binding protein (Tbp ...
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bioRxiv - Developmental Biology 2021Quote: ... The synthesized cDNAs were then employed as templates for PCR amplification using the ChemQ Universal SYBR qPCR Master Mix (Vazyme, Q711-02-AA) and a StepOne Plus Real-Time PCR System (Applied Biosystems) in the following program ...
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bioRxiv - Developmental Biology 2020Quote: ... qPCR reactions were prepared using SYBR Green Master Mix (Applied Biosystem) and run on a 7500 Real-Time PCR Systems (Applied Biosystems). A list of the primers used was provided in Supplementary information ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and oligonucleotide primers (Eurofins Genomics, Louisville, KY; Tables 1 and 2) on a 7500 Real-Time PCR system (Thermo Fisher Scientific). For X ...
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bioRxiv - Developmental Biology 2022Quote: ... using KAPA SYBR FAST qPCR Master Mix (2X) ABI Prism (Thermo, KK4617) on the QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems) thermal cycler ...
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bioRxiv - Biochemistry 2022Quote: ... Reactions were assembled in MicroAmp Optical 96-well reaction plates and carried out in a QuantStudio 7 Flex real-time PCR instrument (Applied Biosystems). PCR conditions were as follows ...
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bioRxiv - Neuroscience 2021Quote: ... Real-time Taqman PCR was performed on an ABI 7900HT with TaqMan Gene Expression Assays (Applied Biosystems, Life Technologies, Carlsbad, CA) for human DAGLB exon 9-10 (Hs00373700_m1) ...
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bioRxiv - Neuroscience 2021Quote: ... Real-time Taqman PCR was performed on an ABI 7900HT with TaqMan Gene Expression Assays (Applied Biosystems, Life Technologies, Carlsbad, CA) for human DAGLB exon 9-10 (Hs00373700_m1) ...
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bioRxiv - Molecular Biology 2019Quote: ... cDNA was used for qPCR using the PerfCTa SYBR Green FastMix (QuantBio) on a Step One Plus Real Time PCR System (Life Technologies) using primer pairs in Supplementary Table 4 ...
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bioRxiv - Molecular Biology 2020Quote: ... To quantify viral genome copy number total DNA was extracted from cells and subjected to qPCR on 7900HT Fast Real-Time PCR System (catalog no: 4329003, Thermo-Fisher Scientific) using Fast SYBR Green Master Mix (catalog no ...
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bioRxiv - Molecular Biology 2020Quote: ... and GADD45 were measured using a StepOne Plus real-time thermocycler with a Fast SYBR Green PCR master mix (4385612; Applied Biosystems) in the amygdala during early life and adulthood ...
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bioRxiv - Molecular Biology 2020Quote: ... The reaction mixtures containing SYBR Green were composed following the manufacturer’s protocol and then CT values were obtained using a qPCR platform (QuantStudio 6 Flex Real-Time PCR System, ThermoFisher Scientific). Real-time PCR data was monitored using QuantStudio 6 Design and Analysis Software Version 2.3 ...
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miR-125-chinmo pathway regulates dietary restriction dependent enhancement of lifespan in DrosophilabioRxiv - Molecular Biology 2020Quote: ... Each cDNA sample was diluted 1:25 and real-time quantitative PCR (qPCR) was performed in duplicate using miRNA-specific primers/probe on a Quant 6 studio Real Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... and subsequently used for qPCR with the PerfCTa SYBR Green FastMix (QuantBio, 95071-012) on a Step One Plus Real Time PCR System (Life Technologies) using primer pairs in Supplementary Table 3 ...
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bioRxiv - Cell Biology 2022Quote: ... using primer pairs that spans either introns or exons of the target genes with an a StepOnePlus Real-Time PCR System (Applied Biosystems). The PCR reactions were performed at 95 °C for 10 min followed by 45 cycles of 95 °C for 15 sec ...
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bioRxiv - Cell Biology 2022Quote: ... 900nM reverse primer and 250nM probe per reaction and 1μL template on a StepOnePlusTM real-time PCR system (Thermo Fisher Scientific). After a UNG incubation at 50°C for 2 minutes Initial denaturation at 95°C for 2 minutes was followed by 40 cycles of 95°C denaturation for 1 second and 60°C anneal-extension for 20 seconds ...
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bioRxiv - Immunology 2021Quote: ... was performed using Thunderbird SYBR qPCR Mix (QPS-201; TOYOBO) with the 7500 fast Real-time PCR system or Quant-Studio 3 (Applied Biosystems). For quantification of gene expression levels ...
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bioRxiv - Biochemistry 2019Quote: ... and fluorescence of the hydrophobic residue-binding dye SYPRO Orange (5000-fold dilution) measured on a Step One Plus Real-Time PCR instrument (Applied Biosystems). DSF measurements were performed for each protein in the absence and presence of SAM (150 μM ...
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bioRxiv - Physiology 2019Quote: ... mRNA levels of the two targets (GFAP and AQP4) by qPCR was performed with the QuantStudio 3 Real-Time PCR system (Applied Biosystems). Brain tissues of interest (WKY ...
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bioRxiv - Immunology 2020Quote: The expression of mRNA levels of IL-1β and SAA3 in BMDMs and lung homogenate cells was measured by Real-Time PCR from the total RNA using GAPDH as internal control with specific primer and probe sets (Applied Biosystems) as previously described (19).
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bioRxiv - Immunology 2019Quote: ... The Tmem119 gene expression was detected by real-time quantitative PCR using TaqMan Fast Advanced Master Mix (#4444557; Thermo Fisher Scientific) and Tmem119 TaqMan Gene Expression Assay (#Mm00525305_m1 ...
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bioRxiv - Biochemistry 2019Quote: ... Real-time RT-PCR was performed in duplicate with AccuPower 2X Greenstar qPCR Master Mix (Bioneer) on a 7900HT Fast Real-Time PCR System (Applied Biosystems). Data were normalized to CD14 ...
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bioRxiv - Biochemistry 2020Quote: Differential scanning fluorimetry (DSF or thermal shift assay) for the DHTKD1 and its variants was performed on QuantStudio 3 real-time PCR system (Applied Biosystems). Purified proteins were diluted to a final concentration of 0.5 mg/ml in 100 mM potassium phosphate ...
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bioRxiv - Genomics 2019Quote: ... Gene expression was determined via Taqman qPCR assays (Table S5) and run on the Applied Biosystems ViiATM 7 Real Time PCR system (ThermoFisher Scientific).
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bioRxiv - Genetics 2019Quote: ... Quantitative real-time PCR analysis using the comparative Ct method was performed on the Applied Biosystem Vii 7 system (ThermoFisher Scientific) with the Powerup SYBR Master Mix (ThermoFisher Scientific ...
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bioRxiv - Genetics 2019Quote: ... height was recorded using a portable GPM anthropometer (Pfister Imports, New York, NY) and rs373863828 was genotyped using a TaqMan real-time PCR assay (Applied Biosystems), as previously described (Minster et al. ...
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bioRxiv - Microbiology 2019Quote: ... Each reaction was performed in technical triplicates and Ct values were generated on a Viia7 Real-time PCR machine (Life Technologies). Relative expression for Itgal was calculated by using ΔΔCt for each sample relative to B-actin ...
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bioRxiv - Immunology 2019Quote: ... cDNAs were mixed with primer pairs and amplified using SYBR green master mix and the 7900HT Fast real-time PCR system (Applied Biosystems). Iμ ...
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bioRxiv - Neuroscience 2019Quote: ... Primer efficiencies for real-time quantitative PCR (qPCR) experiments were calculated using cycle threshold (CT) values (SYBR® Green; Life Technologies) derived from five ...