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Citations for Thermo Fisher :
6601 - 6650 of 10000+ citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... The cells were transfected using Lipofectamine 2000 with TFEB-luc and FL-ERR plasmids (Fisher Scientific, 31985088). 24 hours post-transfection the cells were treated with ERR agonist or DMSO control ...
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bioRxiv - Molecular Biology 2023Quote: ... and transiently transfected with cDNA plasmids using Lipofectamine 2000 transfect reagent (Thermo Fisher Scientific, Waltham, MA, USA) diluted in Opti-MEM reduced serum medium (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... cultures were transfected either with a GFP plasmid using Lipofectamine Stem Reagent (Life Technologies, cat. no. STEM00003) or BacMam GFP (Life Technologies ...
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bioRxiv - Bioengineering 2023Quote: For LiveSR imaging: The tubulin-GFP plasmid was transfected into U2OS cells with Lipofectamine 3000 (Invitrogen, L3000) under the standard protocol.
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bioRxiv - Biochemistry 2023Quote: ... cells were transfected with the ECibN and BSD-CryC plasmids at 30% confluency using lipofectamine 3000 (Invitrogen) with the same DNA ratio ...
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bioRxiv - Cell Biology 2023Quote: ... Cells with stably integrated plasmids were selected with 2 µg/mL puromycin (Gibco™, Thermo Fisher Scientific). Single cell clones were generated by limited dilution ...
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bioRxiv - Cell Biology 2023Quote: ... Cells with stably integrated plasmids were selected with 2 µg/mL puromycin (Gibco™, Thermo Fisher Scientific). Single cell clones were generated by limited dilution ...
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bioRxiv - Cell Biology 2023Quote: ... radixin-GFP or moesin-GFP plasmids 4h before observation using a Neon® MP5000 electroporation system (Invitrogen) with the following parameters ...
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bioRxiv - Biochemistry 2023Quote: ... Bacmids were isolated from 30 mL of culture using PureLink HiPure Plasmid Midiprep Kit (Thermo Fisher # K210004). 25 mL of ExpiSf9 cells at 2.5 x 106 cells/mL in ExpiSf CD medium in a 125 mL non-baffled vented PETG flask (Thermo Fisher # 4115-0125 ...
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bioRxiv - Biophysics 2023Quote: ... plasmid and 1.5 μg/well sheared salmon sperm DNA were transfected using Lipofectamin 3000 (Thermo Fisher Scientific) following the manufacturer’s protocol in 6-well plates ...
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bioRxiv - Cell Biology 2023Quote: ... The transfections for mammalian expression plasmids were performed using Lipofectamine 2000 DNA Transfection Reagent (Thermo Fisher Scientific) following the manufacturer’s instructions.
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bioRxiv - Biophysics 2023Quote: ... The reference vector pRL-TK Renilla plasmid was purchased from Promega UK Ltd and was co-transfected with each of the Firefly plasmids using Lipofectamine 2000 (Thermo Fisher). Each well of the 6-well plate was transfected with a total DNA amount of 3 µg with a 9:1 Firefly to Renilla ratio and a 1:1 DNA to Lipofectamine 2000 ratio ...
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bioRxiv - Microbiology 2023Quote: ... coli strains with one of the following kits: GeneJet plasmid miniprep kit (catalog number K0503; Thermo Scientific). QIAprep Spin Miniprep Kit (catalog number 27104 ...
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bioRxiv - Cancer Biology 2023Quote: ... each plasmid construct was subjected to a cotransfection with pOG44 vector (Thermo Fisher Scientific, Waltham, MA, USA) expressing the Flp recombinase to produce stably transfected Flp-InTM CHO cells (Thermo Fisher Scientific ...
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bioRxiv - Pathology 2023Quote: ... PrP-deficient RK13 cells (ATCC) were transfected with 10 μg of plasmid DNA using Lipofectamine 3000 (Invitrogen). At 24 hours post-transfection ...
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bioRxiv - Immunology 2023Quote: ... Expi293 cells were transfected with plasmids encoding Ig heavy and light chain pairs with ExpiFectamine (ThermoFisher Scientific). Monoclonal antibodies were purified from the cell supernatant using sepharose Protein A (Pierce).
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bioRxiv - Genetics 2023Quote: ... TA cloning was conducted using the recommended protocol and plasmids were transformed into TOP10 cells (C404003; Invitrogen). Each qPCR reaction included approximately 10 ng of cDNA ...
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bioRxiv - Cell Biology 2023Quote: ... the promoter reporter vector and the pRL-CMV Renilla luciferase plasmid using Lipofectamine 2000 reagent (Invitrogen, USA). After transfection for 24 h ...
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bioRxiv - Molecular Biology 2023Quote: ... a pX459 plasmid containing a gene-specific guide RNA (gRNA) was transfected using Lipofectamine 2000 (ThermoFisher Scientific) according to the manufacturer’s instructions (see Table S2 for gRNA sequences used in this study) ...
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bioRxiv - Microbiology 2023Quote: ... The pNCA MLV and p12-M63-PM15 plasmids were transfected into 293T cells using Lipofectamine 3000 (Invitrogen). The media of the transfected cells were harvested 48 h post-transfection ...
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bioRxiv - Cancer Biology 2023Quote: Plasmids were transiently transfected into MCF7 cells using Lipofectamine 3000 according to the manufacturer’s protocol (ThermoFisher, L3000015). After three days ...
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bioRxiv - Biophysics 2023Quote: ... 100 ng/well pSems-mEGFP-TMD plasmid and 2.25 μg/well sheared salmon sperm DNA (#AM9680, Invitrogen) or 500 ng/well of the pN1-GPI-mEos3.2 ...
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bioRxiv - Cell Biology 2023Quote: Plasmids containing the guides and donor templates were transfected in HeLa cells using Lipofectamine 2000 (Invitrogen, 11668030) following manufacturer recommendations ...
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bioRxiv - Molecular Biology 2023Quote: ... two 500ng ligated PX458 plasmid were transfected into cells using Lipofectamine® 2000 (Thermo Fisher Scientific, 11668019) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... cells were transfected with Gluc-CXCR4 plasmid using Lipofectamine™ 3000 Transfection Reagent (Thermo Fisher Scientific, USA). 100 ng/mL Doxycycline (Dox ...
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bioRxiv - Bioengineering 2022Quote: ... and a Cas9 plasmid at a ratio of 1:1:1 (w/w) using Lipofectamine 2000 (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were then transfected with a Cas9-BSD plasmid using the FreeStyle Max transfection reagent (Thermo Fisher), according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... cells were transfected with 10 mg of plasmid using Lipofectamine in Opti-MEM (both Thermo Fisher Scientific). To control for transfection efficiency ...
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bioRxiv - Cell Biology 2022Quote: ... 293T cells were transfected with plasmids using Lipofectamine 2000 according to the manufacturer’s protocol (Life Technologies, Inc.)
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bioRxiv - Cell Biology 2022Quote: ... Plasmid transfections were performed using Lipofectamine 3000 according to the manufacturer’s protocol (catalog number L3000008; Thermo Scientific). siRNA transfections were performed using Lipofectamine RNAiMAX according to the manufacturer’s protocol (catalog number 13778100 ...
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bioRxiv - Biophysics 2022Quote: Plasmid was transfected to suspension Expi293F cells using Expifectamine 293 Transfection Kit (Gibco, Thermo Fisher, Cat # A14524) according to the manufacturer’s instruction ...
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bioRxiv - Biophysics 2022Quote: Plasmid was transfected to suspension Expi293F cells using Expifectamine 293 Transfection Kit (Gibco, Thermo Fisher, Cat # A14524) according to the manufacturer’s instruction ...
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bioRxiv - Developmental Biology 2023Quote: J1 mESCs were transfected with plasmids pAW63-Tex10 and PX330-Tex10 using Lipofectamine 3000 (Thermo Fisher, L3000001) according to the manufacturer’s manual ...
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bioRxiv - Genetics 2023Quote: ... and cloned into a plasmid using the Zero Blunt TOPO PCR Cloning Kit (Thermo Fisher Scientific; 450245). Plasmid DNA was purified using the QIAprep Spin Miniprep Kit (Qiagen ...
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bioRxiv - Cancer Biology 2023Quote: ... The plasmids and siRNAs transfected into cells were carried out by lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA). The sequences of shRNA and siRNA were listed in Supplementary Table 1.
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bioRxiv - Molecular Biology 2023Quote: ... Cells were then transfected with 200μg/mL HA-KDM5-expressing plasmid DNA using the Effectene II (Invitrogen) kit according to the manufacturers protocol ...
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bioRxiv - Bioengineering 2023Quote: ... Plasmids from four colonies with the expected electrophoretic pattern after analytical digestion with NcoI/BamHI (FastDigest, Thermofisher) were Sanger-sequenced by Eurofins Genomics ...
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bioRxiv - Cell Biology 2023Quote: Cells were transfected with plasmids and siRNAs using Lipofectamine 3000 (L3000015, Thermo Fisher Scientific, Waltham, MA, USA) and Lipofectamine RNAiMAX (13778150 ...
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bioRxiv - Biochemistry 2023Quote: ... plasmid DNA (10 µg) and Lipofectamine 2000 (25 µg) were diluted in Opti-MEM (GIBCO 31985-062), combined ...
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bioRxiv - Molecular Biology 2023Quote: ... Frozen Escherichia coli plasmid glycerol stocks containing ACE2 were streaked onto Luria-Bertani (LB) agar plates (Invitrogen) containing 100 µg/mL of Carbenicillin (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... 1.5ug of each plasmid was transfected independently into HEK293 cells using lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA). Forty-eight hours after transfection ...
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bioRxiv - Neuroscience 2023Quote: ... Plasmids were transfected to HAP1 cells using FUGene according to manufactures protocol in Optimem Media (Gibco 31985070). Transfected cells were selected with G418 selection 600 µg/mL in growth media 48 hours after transfection media was added ...
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bioRxiv - Cell Biology 2023Quote: ... Transient transfections were done on these dishes using 2.5ug plasmid and 6ul Lipofectamine 3000 (Thermo Fisher L3000008) two days before imaging ...
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bioRxiv - Developmental Biology 2024Quote: ... 4 μg each expression plasmid was transfected to each well in 500 μl of OptiMEM (Invitrogen, 31985070) and 10 μl Lipofectamine™ 2000 Transfection Reagent (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... and 55 ng of the digested plasmid was religated using 1 U of T4 Ligase (Thermo Scientific) resulting in a plasmid termed pSpCas9(BB) ...
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bioRxiv - Microbiology 2023Quote: ... All molecular biology kits (PCR purification, gel extraction and plasmid miniprep) were also provided by Thermo Fisher Scientific.
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bioRxiv - Molecular Biology 2023Quote: ... cells were transfected with 1 µg each of the pX330 and donor plasmids using Lipofectamine 2000 (ThermoFisher). After 72 hours ...
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bioRxiv - Biochemistry 2023Quote: ... cloned plasmids were linearized with the restriction enzyme PmeI and transformed into Pichia pastoris SMD1168 cells (Invitrogen) by electroporation ...
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bioRxiv - Bioengineering 2023Quote: ... phaffii genes encoding fluorescent proteins were delivered in plasmids pM-GFP and pM-mCH (Thermo Fisher Scientific). Constitutive expression cassettes for GFP and mCherry (mCH ...
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bioRxiv - Cell Biology 2023Quote: ... The resulting PCR fragments were cloned into XhoI-linearized pT-Rex-C-GFP plasmid (Invitrogen, CA, USA) using In-Fusion HD cloning (Takara Bio ...