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Citations for Thermo Fisher :
6401 - 6450 of 10000+ citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2024Quote: Amyloid-β 40 (Aβ40) in the brain was quantified using a human Aβ40 ELISA kit (Invitrogen, USA, #KHB3482) in accordance with manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: ... and human embryonic kidney HEK293T (ATCC, catalog #: CRL-3216) cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2024Quote: ... The human monocytic THP-1 cell line (from ATCC) was cultured in RPMI1640 medium (Thermo Fisher Scientific Inc.) supplemented with 10% FBS and stimulated with OxLDL1 and OxLDL2 at a concentration of 20 µg/mL.
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bioRxiv - Immunology 2024Quote: ... IL-18 and human IL-1β was analyzed in the cell culture supernatant by commercial ELISA kits (Invitrogen) following the manufacturer’s recommended procedures.
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bioRxiv - Neuroscience 2024Quote: Human iPSCs from the WTC11 background were cultured in StemFlex Medium (GIBCO/Thermo Fisher Scientific; Cat. No. A3349401). Human iPSCs from the GIH6C1 background were cultured in mTeSR Plus medium (StemCell Technologies ...
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bioRxiv - Genetics 2024Quote: Human embryonic kidney (HEK)-293T cells (ATCC CRL-3216) were cultured in 10% FBS DMEM (Gibco 11965-092), supplemented with penicillin (50 U per mL ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with with 5% human platelet lysate (hPL, Stemmatters, Portugal) and 1% Penicillin-Streptomycin (P/S, Invitrogen USA). ASCs were maintained at 37°C and 5% CO2 ...
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bioRxiv - Cancer Biology 2021Quote: ... This adapted SMNDC1 reporter was transfected with 2.5 ug plasmid and 10 uL Lipofectamine 2000 (Thermo Fisher) in triplicate in MDA-MB-231 cells with modulated tRNAIle levels ...
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bioRxiv - Molecular Biology 2020Quote: ... H3t-expressing plasmid (pcDNA4/TO/puro-H3t) was introduced into HEK293T cells using Lipofectamine 3000 reagent (Invitrogen), and after 72 h ...
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bioRxiv - Cell Biology 2020Quote: ... The resulting amplicon from overlapping PCR was cloned to entry plasmid pDONR 221 (ThermoFisher, Waltham, Massachusetts, USA), using BP recombination reaction using Gateway technology (ThermoFisher ...
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bioRxiv - Genetics 2021Quote: The plasmid library was transfected in triplicates to 90 million cells/replicate using Neon transfection system (Invitrogen). The cells were detached with trypsin ...
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bioRxiv - Genetics 2020Quote: ... The human PROS1 cDNA (GenBank NM_000313.4) was PCR-amplified from HepG2 cDNA and cloned into pcDNA3.1/myc-His(−) plasmid (Invitrogen). The PCR amplification of PROS1 from c.-44 to c.2028 was performed using Phusion High Fidelity (HF ...
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bioRxiv - Molecular Biology 2021Quote: ... The designed oligos were annealed and ligated into the gRNA plasmid pX459 digested by BbsI (Thermo Scientific). The gRNAs were generated using the MEGAshortscript T7 transcription kit (Ambion ...
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bioRxiv - Developmental Biology 2020Quote: ... 1.5 μg each of plasmid were transiently transfected into E14tg2A mouse stem cells using Lipofectamine 2000 (ThermoFisher) in suspension according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: Capped sense mRNA was generated from linearized plasmid using the mMessage mMachine SP6 & T7 Transcription Kits (Invitrogen). mRNA was microinjected at the 16-cell stage at 200ng/μl ...
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bioRxiv - Genomics 2020Quote: ... and transiently transfected with plasmids after 24 hrs at 75% confluency using Lipofectamine 3000 (Thermo Fisher Scientific). Cells were harvested 24 hrs post-transfection and proteins were purified with c-Myc tagged Protein Mild Purification Kit (MBL ...
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bioRxiv - Cell Biology 2022Quote: ... All the siRNA silencing and plasmid transfection studies was done with lipofectamine 2000 purchased from Thermo Fisher Scientific.
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bioRxiv - Biophysics 2022Quote: ... the plasmids (Supplementary Table 1) and the Lipo2000 were diluted into Opti-MEM Reduced Serum Medium (Gibco), respectively ...
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bioRxiv - Neuroscience 2021Quote: ... The plasmid DNA was transformed in chemically competent bacteria (One Shot® Stbl3™, Thermo Fisher Scientific) following the manufactureŕs protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... then transfected with a Cre-recombinase plasmid using Lipofectamine 2000 according to the manufacturer’s protocol (Thermo Fisher), to delete the puromycin resistance cassette ...
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bioRxiv - Molecular Biology 2020Quote: ... Sequences of all plasmid inserts were verified and transferred into pAM4418 using the Gateway recombination system (Invitrogen) and Invitrogen’s LR Clonase II Enzyme Mix ...
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bioRxiv - Molecular Biology 2021Quote: ... the linear plasmid was obtained by treatment with a single cut restriction endonuclease (XhoI, Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2020Quote: ... Plasmids were transfected into cells using Lipofectamine 2000 reagent (Invitrogen, Carlsbad, CA, USA; cat. no. 11668-019) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2021Quote: ... The plasmid mixtures were combined with 0.54 ul of TransIT-X2 and volume of Opti-MEM (ThermoFisher) to a final volume of 15 ul ...
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bioRxiv - Cell Biology 2022Quote: ... the MDA-MB-231 cell line was transfected with the obtained plasmid DNA using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... the MDA-MB-231 cell line was transfected with the obtained plasmid DNA using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... The resulting plasmids of heavy and light chain pairs were co-transfected in Expi293F cells (Thermo Fisher) using Turbo293 transfection reagent (Speed BioSystems ...
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bioRxiv - Immunology 2021Quote: ... Cells were transfected with 15 ug sgRNA plasmid using 30 μL of Lipofectamine 2000 (Invitrogen, Cat#11668019). Plat-E cells were further cultured for 48 hours ...
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bioRxiv - Molecular Biology 2021Quote: ... 1000 ng of plasmid DNA was transfected into each well using Lipofectamine 2000 (Thermo Fisher Scientific 11668019) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... see Table 1 for complete plasmid list) were first made using the Gateway LR Clonase system (ThermoFisher). Specifically ...
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bioRxiv - Cancer Biology 2019Quote: ... and 9μg of lentiviral plasmids were co-transfected into 293FT cells using 36μl Lipofectamine 2000 reagent (Invitrogen). Forty-eight hours later ...
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bioRxiv - Neuroscience 2019Quote: ... Pamela McLean) and 3 μg of red firefly expressing plasmid (pCMV-Red firefly Luc, ThermoFisher, Waltham, MA) per dish ...
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bioRxiv - Biochemistry 2019Quote: ... 5 μg (otherwise noted) of terminally tagged and unmodified σ1R plasmid was transfected using lipofectamine 2000 (Invitrogen) for HEK 293T Δσ1R cells in a 10 cm plate ...
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bioRxiv - Microbiology 2019Quote: ... and NS and a PB2 protein-expression plasmid using Lipofectamine™ 3000 Transfection Reagent (Invitrogen, California, USA). The supernatant was collected on day 2 post-infection and passaged twice in the AX4/PB2 cell line ...
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bioRxiv - Plant Biology 2019Quote: ... The ZmIAA genes were inserted into pGP4GY plasmids (Havens et al., 2012) via Gateway cloning (Life Technologies), or by Gibson cloning (Gibson et al. ...
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bioRxiv - Cell Biology 2019Quote: ... 1mg sgRNA plasmid was transfected into 9×104 Ng2S cells by Lipofectamine 2000 Transfection Reagent (11668019, Invitrogen). Culture medium was refreshed after 12 h to remove Lipofectamine 2000 Transfection Reagent ...
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bioRxiv - Bioengineering 2020Quote: ... UK) and the piggyBac-BE3-GFP plasmid at a 1:4 molar ratio using Lipofectamine 3000 (Invitrogen). At 48 h after transfection ...
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bioRxiv - Developmental Biology 2019Quote: The amplified products were cloned into the pENTR plasmid containing UASp and 3xHA (Life Technologies, cat# K240020), digested with EcoRI and BglII ...
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bioRxiv - Developmental Biology 2019Quote: ... 1 μg NotI-linearised pCS2-TP plasmid was transcribed using a SP6 mMESSAGE mMACHINE kit (ThermoFisher Scientific) according to manufacturer’s protocol.
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bioRxiv - Cell Biology 2019Quote: ... Cells were then incubated for two days then transfected with specified plasmids or siRNA using TurboFect (ThermoFisher) followed by overnight incubation ...
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bioRxiv - Immunology 2021Quote: ... 1×107 THP1 cells were transiently transfected with 50μg of respective pX458 plasmids using Lipofectamine LTX (Invitrogen) and incubated overnight ...
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bioRxiv - Genomics 2020Quote: ... Final plasmids were purified with phenol/chloroform and concentration was determined by NanoDrop 1000 Spectrophotometer (ThermoFisher Scientific).
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bioRxiv - Cell Biology 2021Quote: ... Transfections were performed overnight 36 h preceding the experiment using 0.2 µg plasmid DNA and 0.2 µL Lipofectamine P3000 solution following the manufacturer’s instructions (ThermoFisher). The media was exchanged to standard culture medium 12 h post transfection ...
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bioRxiv - Cell Biology 2021Quote: WT and Vim-/- MEFs are transfected with an EGFP-actin plasmid using Lipofectamine 2000 transfection agent (Invitrogen) and imaged on the third day ...
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bioRxiv - Cell Biology 2021Quote: ... and GBM cells were transfected with plasmid DNA using Lipofectamine 2000 Stem reagent (Thermo Fisher, Cat# STEM00008), following the manufacturer’s protocol.
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bioRxiv - Biophysics 2021Quote: ... The plasmid construct was transformed into an electrocompetent strain of Escherichia coli DH10β cells (ThermoFisher Scientific, USA) via electroporation ...
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bioRxiv - Microbiology 2021Quote: A pooled sample of plasmid extracts from Synechocystis was subjected to rectriction by NcoI (Thermo Scientific: FD0573), which has a single cut site in pCA2.4_M but not in the similarly sized pCB2.4_M ...
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bioRxiv - Biochemistry 2020Quote: ... each well of one plate was individually transfected with the TRPC4DR plasmid using Lipofectamine (Thermo Fisher Scientific). The other plate was left untransfected and used as a control ...
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bioRxiv - Biochemistry 2020Quote: ... 3.0 μg plasmid was transfected into ~2 × 106 MCU/EMRE knockout cells [16] using Lipofectamine 3000 (Invitrogen). For MCU/EMRE co-expression ...
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bioRxiv - Neuroscience 2020Quote: ... Final constructs were subcloned into mammalian expression plasmids pIR-CMV (NaV1.1, NaV1.2) (18) or pcDNA4/TO (NaV1.6; ThermoFisher). Each construct included an internal ribosome entry sequence (IRES2 ...