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Citations for Thermo Fisher :
6451 - 6500 of 10000+ citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... Cells were then transfected with 400 ng of DNA plasmid prepared in OPTI-MEM Reduced Serum (Gibco), using Lipofectamine LTX with Plus-Reagent ...
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bioRxiv - Cancer Biology 2019Quote: ... Where indicated plasmid expressing wild type DHX9 or S990A mutant was transfected using lipofectamine 2000 reagent (Invitrogen) for 48 hours ...
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bioRxiv - Cell Biology 2021Quote: ... HeLa cells were transfected with the indicated plasmids for 3h in serum depleted medium (Opti-MEMTM, ThermoFisher) using lipofectamine 2000 (Life Technologies) ...
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bioRxiv - Plant Biology 2021Quote: Plasmids were amplified by PCR using the high fidelity Phusion Hot Start II (Thermo Fisher Scientific®) taq polymerase and the indicated primers carrying mutations (Table S6) ...
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bioRxiv - Cell Biology 2021Quote: ... RPE1 cells were transfected with the plasmids using Lipofectamine LTX according to the manufacturer’s instructions (Life Technologies). HEK293T cells were transfected with the plasmids using 1 μg/μl polyethylenimine ...
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bioRxiv - Plant Biology 2020Quote: ... and cloned into the intermediate pTwist plasmid through EcoRI restriction and T4 DNA ligation (ThermoFisher Scientific, USA), downstream of the SanCas9 coding sequence ...
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bioRxiv - Plant Biology 2021Quote: ... The pENTR-ARF19 vector was recombined into the pEarleyGate104 (35S:YFP-GW) plasmid (17) using LR Clonase (Invitrogen). Recombinant plasmid was transformed into Agrobacterium tumefaciens strain GV3101 (18) ...
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bioRxiv - Plant Biology 2021Quote: ... Plasmids used for yeast two-hybrid or Co-IP were generated by gateway LR cloning (Life Technologies) using the ENTRY vectors described above and appropriate destination vectors listed in Suppl ...
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The heterogeneity of the DNA damage response landscape determines patient outcomes in ovarian cancerbioRxiv - Cell Biology 2021Quote: ... Explant plasmid transfections were prepared with lipofectamine LTX Plus or 3000 (15338-100, 11668-027; Thermo Fisher) using Opti-Mem (11058021 ...
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bioRxiv - Neuroscience 2020Quote: ... CCM2 isoforms were inserted into pcDNA3.1/V5-His-TOPO and Topo pCR®II plasmid vectors (Invitrogen) respectively (1-6 ...
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bioRxiv - Microbiology 2021Quote: ... linearized (BamHI/XbaI digest) pCG1 plasmid and GeneArt™ Gibson Assembly HiFi Master Mix (Thermo Fisher Scientific). Gibson assembly was performed according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: HEK293T cells were transfected with each plasmid expressing an EBV gene using Lipofectamine 2000 (Thermo Fisher Scientific). On the following day ...
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bioRxiv - Immunology 2021Quote: ... Plasmids encoding Ig heavy and light chain pairs were co-transfected using Polyethylenimine into 293FreeStyle cells (Invitrogen). Supernatants were assayed for secreted rAb and for HA reactivity using the HA CBA described above ...
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bioRxiv - Immunology 2020Quote: ... These plasmids were then transfected into Raw 264.7 cells using a Neon Transfection System (Thermo Fisher Scientific) with the following parameters ...
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bioRxiv - Microbiology 2021Quote: ... 2.5 μg of plasmid DNA was transfected into keratinocytes using Lipofectamine 3000 (Invitrogen; Thermo Fisher Scientific, Singapore), according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... 2.5 μg of plasmid DNA was transfected into keratinocytes using Lipofectamine 3000 (Invitrogen; Thermo Fisher Scientific, Singapore), according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Cells were transfected with 1 µg of plasmid DNA and 2 µL of Lipofectamine 2000 reagent (Invitrogen). Six hours post transfection ...
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bioRxiv - Microbiology 2021Quote: ... pT7 Fluc plasmid was linearized with XmnI and mMessage mMachine mRNA synthesis kit (Life Technologies, Burlington, Canada) was used for invitro transcription (All the transcription process was performed using suggested manufacturer’s protocol.
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bioRxiv - Cell Biology 2020Quote: ... The transient transfection of plasmids and siRNAs were performed using Lipofectamine 2000 reagent (ThermoFisher Scientific, # 11668-500) and Dharmafect-1 (Dharmacon ...
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bioRxiv - Cancer Biology 2021Quote: ... CA) plasmid were co-transfected at a ratio of 2.5:1 using Lipofectamine 3000 (Thermo Fisher Scientific). After incubation with the transfection complexes for 48 hours ...
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bioRxiv - Cell Biology 2020Quote: ... RAW cell lines stably expressing LifeAct were generated using a PEF6_GFP_LifeAct plasmid and maintained under Blasticidin (ThermoFisher) selection.
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bioRxiv - Cell Biology 2020Quote: ... Transfections with plasmids in C3H10T1/2 were done by using Lipofectamine LTX with PLUS™ Reagent (Invitrogen). Transient transfections were done in 293T cells by using calcium phosphate method ...
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bioRxiv - Cell Biology 2021Quote: Cells were transfected with 1 µg of plasmids using electroporation with Neon Transfection System (Thermo Fisher Scientific) using the following parameters ...
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bioRxiv - Cell Biology 2022Quote: ... the AGO1 complemented Ago1_KO2 cells were obtained by stably transfecting the pMSCV_PIG_3xHA-AGO1 plasmid (addgene #170916) with lipofectamine 3000 (Invitrogen). Cells were grown for one week under puromycin selection and then sorted by FACS to select only GFP expressing cells ...
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bioRxiv - Developmental Biology 2022Quote: Transfection of DNA plasmids into the chicken fibroblast line cells DF1 was performed using Lipofectamin 3000 (Invitrogen). Three kinds of transfected cells were separately prepared introduced with ...
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bioRxiv - Biochemistry 2022Quote: ... hPolεCD(insect) with a cleavable N-terminal His-Tev tag was cloned into pFastBac-1 plasmid (Invitrogen). hPolεCD and hPolεCD(exo- ...
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bioRxiv - Biochemistry 2022Quote: ... Individual clones from each reaction were generated using the GeneJET Plasmid Miniprep Kit (ThermoFisher Scientific, Waltham, MA) and sequenced to confirm the sequence of each mutated open reading frame and to determine its corresponding 10 base barcode sequence ...
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bioRxiv - Neuroscience 2022Quote: ... The amplified fragments were cloned into pDNR221 to generate p-entry plasmids using Gateway cloning (ThermoFisher Scientific). Lentiviral destination plasmids were generated by the LR recombination between p-entry and destination plasmids.
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bioRxiv - Microbiology 2022Quote: ... were transfected with 500 ng of nsp14-strep plasmid encoding nsp14-strep using Lipofectamine 3000 (Thermo Fisher). The next day ...
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bioRxiv - Genomics 2022Quote: ... and 1.5 µg of dCas9-KRAB repression plasmid per well by Lipofectamine 3000 transfection reagent (Invitrogen; L3000015) per manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... and L plasmids at a ratio of 4:2:2:2:1 using Lipofectamine 2000 (ThermoFisher Scientific). Cells were transfected in 6 well plates and subsequently transferred to T25 flasks with HEp2 cells until cytopathic effect (CPE ...
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bioRxiv - Microbiology 2022Quote: Plasmids and RNA oligonucleotides were transfected into cells in 24-well plates using Lipofectamine 3000 (Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... linearized (BamHI/XbaI digest) pCG1 plasmid and GeneArt™ Gibson Assembly HiFi Master Mix (Thermo Fisher Scientific). Gibson assembly was performed according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... HEK293-PSMB2-HB cells were transfected with 5 μg of the cloned plasmid using Lipofectamine 3000 (Invitrogen). After 48 h incubation ...
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bioRxiv - Microbiology 2021Quote: ... 1 μg of the plasmid encoding full-length TMPRSS2 and 30 μl of Lipofectamine 2000 (Life Technologies) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... we linearized the plasmids as templates for DIG-labeled riboprobes synthesis by T7 polymerase (Thermo Fisher Scientific) with DIG RNA labeling mix (MilliporeSigma).
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bioRxiv - Molecular Biology 2019Quote: ... The reporter (2.5 μg plasmid DNA per well) was transiently transfected using Cellfectin II transfection reagent (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: Plasmids were transfected into HEK293T cells using jetPRIME (Polyplus) and PK15 cells using Lipofectamine LTX Reagent (Invitrogen) following the corresponding manufacturer’s protocol.
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bioRxiv - Microbiology 2021Quote: ... A549 or Vero cells were transfected with 3 μg of plasmids with transfection reagent LipofectamineR 2000 (Invitrogen) according to manufacturers’ instructions 24 h post seeding ...
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bioRxiv - Immunology 2021Quote: ... and 12 µg lentiviralpLVX-EF1α-IRES-mCherry plasmid for TCR expression per T75 flask (Thermo Fisher Scientific). This master mix was added to the diluted Lipofectamine 3000 Reagent and incubated for 15 min at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... and the plasmid inserts were sequenced using Sanger sequencing on an ABI 3730xl DNA Analyzer (ThermoFisher Scientific) at the NYSDOH Wadsworth Center ATGC Core Facility ...
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bioRxiv - Neuroscience 2022Quote: ... miRNA plasmids were prepared using the BLOCK-iT Pol II miR RNAi Expression Vector Kit (ThermoFisher Scientific) with top and bottom sequences targeting mouse Rxra cDNA (Top ...
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bioRxiv - Systems Biology 2022Quote: ... Cells were transfected in suspension ( 70 000 cells/mL) with 500 ng/plasmid using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... Constructs were transfected with the relevant pcDNA5/FRT/TO plasmid and the Flp recombinase pOG44 (Thermo Fisher) using Fugene HD (Promega ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were transfected with either empty vector or the expression plasmid pCMV6-Entry-UBE3C-IF (0.5 µg, 1.0 µg, 2.0 µg) via lipofection using Lipofectamine 3000 (Invitrogen) as per the manufactures protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... RNAs were synthesized from the linearized plasmids using the in vitro mMessage mMachine RNA synthesis kit (Ambion). 100-250pg of Mem-mCherry and utrophin-GFP/RFP RNAs and 1-2ng myoIIB-GFP RNAs were injected into the marginal zone regions of two dorsal blastomeres of 4-to 8-cell stage embryos ...
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bioRxiv - Developmental Biology 2019Quote: ... Plasmids containing both the sense and antisense wee2 3’UTR were linearized using NdeI (Thermo Fisher Scientific) and in vitro transcription was performed using the Message Machine T7 Kit (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2020Quote: ... Table 1 and cloned into the plasmid pGWB13 (Nakagawa et al., 2007) using the Gateway technology (Invitrogen). This plasmid fuses three C-terminal hemagglutinin (HA ...
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bioRxiv - Developmental Biology 2019Quote: ... Tol2 mRNA was synthetized from linearized plasmid using the mMessage mMachine SP6 Transcription kit (Thermo Fisher Scientific) and purified (RNAeasy Mini Kit ...
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bioRxiv - Neuroscience 2019Quote: ... unc-7 rescue plasmids were made using the Multisite Gateway® 3-Fragment Vector Construction Kit (Invitrogen). A 1078bp mec-4 promoter fragment (as previously used ...