Labshake search
Citations for Thermo Fisher :
6551 - 6600 of 10000+ citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2022Quote: ... cells were transfected with 500 ng plasmid/well and a final volume of Lipofectamin 2000 (Thermo Fisher) of 1 µL/well in a total volume of 100 µL OPTI-MEM (Gibco ...
-
bioRxiv - Cell Biology 2022Quote: ... cells were transfected with respective plasmids 36-48 hours before experiments using Neon transfection system (Thermo Fisher) or JetOptimus chemical transfection reagent (PolyPlus ...
-
bioRxiv - Cell Biology 2022Quote: Both plasmids (1.5 μg) were transfected into a fibroblasts cell line with Lipofectamine 3000 (Thermo Fisher Scientific) reagent according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2022Quote: HEK293 FT cells were transfected with dVPR and VSVG packaging plasmids using Lipofectamine 2000 reagent (ThermoFisher Scientific) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2022Quote: ... then transfected for 5 hours with indicated plasmids in 2 mL Opti-MEM (Invitrogen, Cat. #31985-088) using Lipofectamine 3000 transfection kit (Thermo Fisher ...
-
bioRxiv - Cell Biology 2022Quote: ... pLVX-pEF1a-Tet3G Vector plasmid DNA in 1:1 ratio using Lipofectamine LTX with Plus Reagent (Invitrogen). 24hrs post-transfection ...
-
bioRxiv - Genetics 2022Quote: ... together with 10.5 μg of the CRISPRaX library plasmid in 1 ml of Opti-MEM (Life technologies) using 60 μl Lipofectamine 2000 reagent (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2022Quote: ... Cells were transfected with the respective plasmids using Lipofectamine® 2000 according to manufacturer’s instructions (Invitrogen™) and incubated for a further 24 hours ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... The plasmid DNA sequences encoding target proteins were determined using an ABI 3500 (Applied Biosystems, MA, USA). HA-GFP-fusion target protein- and DsRed-expressing HeLa cells were treated with 1% DMSO or CANDDY molecules in serum-free medium for 24 h ...
-
bioRxiv - Microbiology 2022Quote: ... Env accession number JQ867464)43 was cloned into a modified pMT/BiP insect cell expression plasmid (Invitrogen) designated pT350 ...
-
bioRxiv - Microbiology 2022Quote: ... plasmids were transfected in NIH-3T3 cells in a 96-well plate using Lipofectamine ™ 3000 (Invitrogen). Luciferase readings were measured 48 hours’ post-transfection using the Dual-Glo® Luciferase assay system (Promega) ...
-
bioRxiv - Microbiology 2022Quote: ... coli was isolated from overnight cultures grown in LB using the GeneJET plasmid miniprep Kit (Thermo Fisher). DNA was gel purified using a GeneJET gel extraction kit (Fermentas) ...
-
bioRxiv - Molecular Biology 2023Quote: ... Chimeras were made by digesting ACR-16-REM plasmids with the appropriate restriction enzymes (ThermoFisher Scientific, USA) (S Table 1 ...
-
bioRxiv - Cancer Biology 2024Quote: ... packaging plasmid psPAX2 and pMD2G in a ratio of 2:2:1 using lipofectamine 3000 (ThermoFisher, L3000001) and transduced into NES cells and ONS76 cells ...
-
bioRxiv - Neuroscience 2022Quote: ... They were plated on coverslips in 24-well plates and transfected with plasmids using lipofectamine 2000 (Invitrogen). Two days after transfection ...
-
bioRxiv - Neuroscience 2022Quote: ... The cells were transfected with a dmsr-7/pcDNA3.1 plasmid using lipofectamine LTX and Plus reagent (Invitrogen) and grown overnight at 37°C ...
-
bioRxiv - Molecular Biology 2022Quote: Plasmids encoding for c17orf80-BirA* N- or C-terminal fusions were generated using Gateway cloning technology (Invitrogen) using the c17orf80 sequence (NM_001100621 ...
-
bioRxiv - Cell Biology 2022Quote: ... containing 20 ug of plasmid DNA and 3 μL sheared salmon sperm DNA (Thermo Fisher Scientific AM9680). Suspensions were then transferred to individual GenePulser 0.4 cm cuvettes (Bio-Rad 1652088) ...
-
bioRxiv - Developmental Biology 2022Quote: ... 1μg total plasmid DNA (1:3 SAM to gRNA ratio) was diluted in Opti-MEM (Gibco #31985062) and mixed with p3000 reagent ...
-
bioRxiv - Molecular Biology 2022Quote: ... HEK 293 cells were transfected with 2.5 μg of plasmid DNA using Lipofectamine 3000TM (Invitrogen, Paisley, UK). Three days post transfection ...
-
bioRxiv - Molecular Biology 2022Quote: ... coli expression plasmids for purification of recombinant Cdc14 enzymes were generated using the Gateway cloning system (Invitrogen). S ...
-
bioRxiv - Cancer Biology 2022Quote: COS-7 cells were transfected with plasmids encoding the individual HLA molecules using Lipofectamine 2000 (Life Technologies). After resting overnight ...
-
bioRxiv - Molecular Biology 2024Quote: ... The HTCS/carrier plasmid mix was transfected in duplicate 96-well plates using lipofectamine 2000 (Thermo Fisher). Lipofectamine 2000 reagent was prepared for transfection by mixing at 0.3 μL Lipofectamine 2000 per 7.2 μL of OptiMEM (Thermo Fisher) ...
-
bioRxiv - Molecular Biology 2024Quote: ... The PCR fragments were then ligated into pJET1.2/blunt plasmid using CloneJET PCR cloning Kit (Thermo Scientific). To generate plasmids containing 3’-end of PGK1 cDNA 5’ part of PGK1 was excised by NcoI from previously generated full-length PGK1 cDNA plasmids ...
-
bioRxiv - Biochemistry 2024Quote: AriA and AriB-strep were co-expressed from a pRSF-Duet plasmid in BL21-AI cells (Invitrogen). Cultures grown at 37°C to 0.4-0.5 OD600 were induced with 0.1 mM IPTG and 0.1% L-arabinose ...
-
bioRxiv - Cell Biology 2024Quote: ... 0.75-1 µg of sgRNA/Cas9 plasmids was transfected into XC45-6S cells using Lipofectamine LTX (Thermofisher) in a 6-well plate format ...
-
bioRxiv - Microbiology 2024Quote: ... plasmids were linearized and in vitro transcribed with the mMessage mMachine SP6 transcription kit (Invitrogen, cat. AM1340) per the manufacturer’s protocol to produce the full-length positive-sense mRNA which was transfected into Vero cells using Lipofectamine 3000 following the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2024Quote: ... and 40 ng of Cas7-11 expressing plasmid were cotransfected using Lipofectamine 3000 (Thermo Fisher Scientific, L3000001) according to manufacturer standards.
-
bioRxiv - Molecular Biology 2024Quote: Plasmids were transiently transfected in Expi293F cells using polyethylenimine or ExpiFectamine™ transfection reagent (Thermo Fisher Scientific) and encoded the following ...
-
bioRxiv - Molecular Biology 2024Quote: ... cells were co-transfected with 0.5 μg pcDNA5-StFLAG-DSS1 and 4.5 μg recombinase plasmid pOG44 (Invitrogen) for 48 hours ...
-
bioRxiv - Molecular Biology 2024Quote: All DNA plasmids were transfected into Hek-Luc cells using Lipofectamine™ LTX Reagent (Thermo Fisher Scientific). Cells were seeded 24 hours prior to transfection to ensure high viability and suitable confluency ...
-
bioRxiv - Neuroscience 2024Quote: Transfection of plasmids and siRNAs (Supplementary Table) were performed using Lipofectamine 3000 and Lipofectamine 2000 (Life Technologies) following manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2024Quote: ... Linearized plasmid was run off with SP6 RNA polymerase using the mMESSAGE mMACHINE SP6 Transcription kit (Ambion). ptgs1 RNA was injected together with emx2 MO at one-cell stage at concentration 825 pg for rescue study.
-
bioRxiv - Cell Biology 2024Quote: ... Transient transfection of siRNA oligonucleotides (Genepharma, Shanghai) or plasmids was performed using Lipofectamine RNAiMAX (Thermo Fisher, 13778150) in Opti-MEM (Thermo Fisher ...
-
bioRxiv - Developmental Biology 2024Quote: ... Linearized plasmid was run off with SP6 RNA polymerase using the mMESSAGE mMACHINE SP6 Transcription kit (Ambion). ppargc1a RNA was injected together with emx2 MO at one-cell stage at concentration 375 pg for rescue study ...
-
bioRxiv - Genomics 2023Quote: ... and CRISPRmap library plasmid (2:3:4 ratio by mass) using Lipofectamine 3000 (Thermo Fisher Scientific L3000001) in lentiviral packaging medium (Opti-MEM (Thermo Fisher Scientific 31-985-070 ...
-
bioRxiv - Immunology 2024Quote: We performed transfection of siRNA or expression plasmid by using a Neon Transfection System (Thermo Fisher Scientific). BM-derived culture cells (1.5 x 106 ...
-
bioRxiv - Genomics 2024Quote: ... cells were transfected with 1 µg of plasmid library using 3 µl Lipofectamine 3000 reagent (L3000008, Invitrogen, Carlsbad ...
-
bioRxiv - Developmental Biology 2024Quote: ... 2µg MNS1-HA/2µg MNS1-Myc and 2µg CFAP53-HA/2µg CFAP53-Myc plasmids were incubated with 20µl of Lipofectamine 2000 (Invitrogen) in 300 µl Optimal MEM (Invitrogen ...
-
bioRxiv - Immunology 2024Quote: Plasmid DNA was transfected into Calu-3 cells applying Lipofectamine 3000 following supplier’s instructions (Thermo Fisher Scientific). Plasmid DNA was also nucleofected into 721.221 cells using Lonza’s Kit-V by Nucleofector II electroporation-based transfection system according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... Plasmid with or without the gRNA sequence was transfected into LLC-PK1 cells with Lipofectamine 2000 (Invitrogen) and cells were incubated for 48 h ...
-
bioRxiv - Genetics 2023Quote: ... Transformed bacteria were grown overnight and plasmid pools were extracted using the PureLink Midiprep Kit (Invitrogen, K210014). To check library complexity ...
-
bioRxiv - Bioengineering 2024Quote: HEK 293 T cells were transfected with DNA plasmids using Lipofectamine™ 3000 Transfection Reagent (ThermoFisher Scientific) per manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2023Quote: Capped mRNAs were synthesized in vitro from the linearized pOX(+) plasmids using mMessage-mMachine SP6 kit (Invitrogen). Unless stated otherwise ...
-
bioRxiv - Cell Biology 2023Quote: ... The PCR product was cloned into the pJET1.2 plasmid using the CloneJET PCR Cloning Kit (Thermo Scientific), according to the manufactureŕs instructions ...
-
bioRxiv - Genetics 2023Quote: ... To produce 35S::HTA2 construct we first cloned HTA2 gene sequence into pENTR-D/TOPO plasmid (Invitrogen). After the correct sequence of HTA2 was verified by Sanger sequencing ...
-
bioRxiv - Developmental Biology 2023Quote: ... The resulting plasmid was transformed into and amplified in competent DH5α cells (Invitrogen, Cat no. 18263-012), purified (QIAprep Spin Miniprep kit ...
-
bioRxiv - Cell Biology 2023Quote: ... SVG-A or A673 cells were transfected with the gRNA-containing plasmids using Lipofectamine™ 2000 (Invitrogen), and cells were allowed to grow for 3-6 days ...
-
bioRxiv - Synthetic Biology 2023Quote: ... a total of 200 ng of plasmids were diluted in 20 µL of Opti-MEM (Gibco #31985062), and 0.6 µL of Lipofectamine 2000 (Invitrogen #11668019 ...
-
bioRxiv - Molecular Biology 2022Quote: ... as previously shown in (Clément et al., 2018) and for plasmids using Lipofectamine 2000 (Invitrogen, 11668-019). Sequences used for the siRNA are mentioned in the supplementary table 1 ...