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Citations for Thermo Fisher :
6801 - 6850 of 10000+ citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: Human MZF1 cDNA was subcloned from pOTB-MZF1 into pcDNA3-Flag vector via TOPO directional cloning (Thermo Fisher Scientific) and designated pcDNA3/Flag-MZF1 ...
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bioRxiv - Pathology 2020Quote: ... CHO cells were transfected with 5 ug of human cadherin-6 in pIRES-puro or mouse cadherin-6 in pCDNA3.1 via Lipofectamine 2000 (Invitrogen). After 48hrs ...
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bioRxiv - Immunology 2019Quote: ... Plates were washed 6x 300μl PBST and developed with an anti-human Fc specific secondary antibody (Invitrogen, Carlsbad, CA) and TMB as described above ...
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bioRxiv - Immunology 2020Quote: Whole genome transcript analysis was performed on 200 ng of total RNA using the Affymetrix Human Gene ST version 2.0 Array (Affymetrix). Genome-wide DNA methylation was profiled using the Illumina EPIC platform (Illumina ...
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bioRxiv - Physiology 2020Quote: Cryo-preserved primary human hepatocytes from single donors (PHH; BD Gentest, Tewksbury, MA; Lonza, Walkersville, MD; Thermo Fisher Scientific) and the human HCC cell line HuH7 (ATCC ...
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bioRxiv - Immunology 2019Quote: ... The plate was incubated with detection antibody (mouse anti-human IgG1 Fc conjugated to horseradish peroxidase clone HP6069; ThermoFisher) for 2 hours at room temperature ...
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bioRxiv - Immunology 2019Quote: A cDNA encoding wild-type (WT) human TRIM22 (a gift form Professor Juergen Hass, University of Edinburgh) was inserted into pcDNA.3.1 (Invitrogen) in frame with a 5’ Myc-tag oligo to generate a pcDNA.Myc.TRIM22 ...
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bioRxiv - Cell Biology 2020Quote: ... For RHBDL4 and OST4 depletion 50 pmol (RHBDL4) or 25 pmol (OST4) ON-TARGETplus SMARTpool human siRNA (Dharmacon) and 2.5 μl RNAimax (Invitrogen) transfection reagent per well of a 12-well plate were used ...
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bioRxiv - Cell Biology 2019Quote: ... Human telomerase reverse transcriptase immortalized retinal pigment epithelial cells (TERT-RPE1, ATCC CRL-4000) were cultured in DMEM:F12 (Gibco) supplemented with 10% FBS and 1% penicillin and streptomycin ...
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bioRxiv - Cancer Biology 2020Quote: Copy number analysis was derived from profiling of total DNA on the Affymetrix genome-wide human SNP Array 6.0 chip using instrumentation and protocols from Affymetrix. The raw CEL files were returned and the QC steps were performed using Affymetrix Genotyping Console ...
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bioRxiv - Genomics 2019Quote: Human islets were washed once in cold PBS and dissociated into single cells by enzymatic digestion with Accumax (Invitrogen), followed by digestion using freshly prepared Dispase (Fisher Scientific) ...
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bioRxiv - Genetics 2021Quote: ... with 12.5 pmol of either non-targeting negative control siRNA showing no homology to the human transcriptome (#4457287, Ambion), or two different siRNAs targeting OAS1 (siRNA-1 or −2 ...
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bioRxiv - Immunology 2020Quote: ... T cells were polyclonally expanded from freshly isolated PBMCs using Dynabeads human T-activator CD3/CD28 beads (Life Technologies) as previously described (Loef et al ...
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bioRxiv - Molecular Biology 2020Quote: Human HCT116 cells (ATCC) were cultured at 37°C under 5% CO2 in McCoy’s 5A media (Thermo Fisher Scientific) with 1x penicillin/streptomycin and 10% FBS (Invitrogen) ...
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bioRxiv - Synthetic Biology 2020Quote: ... and 1 day prior to spinfection cells were activated using Human T-activator CD3/CD28 Dynabeads (Thermo Fisher, 11131D) and cultured in 200U/ml IL-2 ...
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bioRxiv - Neuroscience 2020Quote: Fresh-frozen human postmortem brain tissues were sectioned at 14-16 μm onto Superfrost Plus glass slides (Fisher Scientific). Sections were dried for 20 minutes at −20°C and then vacuum sealed and stored at −80°C until use ...
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bioRxiv - Cancer Biology 2021Quote: Non-targeting siRNA and siRNA against mouse and human LIG3 were transfected into the cells using Lipofectamine RNAiMAX (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: Human glioblastoma cell line U-251MG was cultured in Dulbecco’s Modified Eagle Medium (DMEM-HG) (11995-065; Gibco, USA) and supplemented with 10% FBS (RM10409 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Gene expression was performed on a BioRad CFX96 real time PCR machine using gene-specific FAM-labelled TaqMan probe assays: human GAPDH control ID: Hs99999905_m1(ThermoFisher), zebrafish abcb4 ID ...
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bioRxiv - Cancer Biology 2020Quote: ... wells were filled with 250μl of human liver organoid isolation medium consisting Ad+++ supplemented of 1X B27 supplement without retinoic acid (Gibco), 1X N2 supplement (Gibco) ...
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bioRxiv - Immunology 2020Quote: ... and resuspended in complete medium in the absence of human serum or Pf-iRBCs schizonts were isolated in RPMI 1640 medium supplemented with 0.25% Albumax (GIBCO, Invitrogen) and 7.4% Sodium Bicarbonate (GIBCO ...
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bioRxiv - Bioengineering 2021Quote: ... Another subset of slides was labeled with Alexa 568 conjugated anti-human IgG antibody (1:800, ThermoFisher, A-21090) to detect the Fc portion of the EphA4-Fc chimera peptide.
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Sapogenin based self-assembly structures activating a non-apoptotic cell death via multiple pathwaysbioRxiv - Pharmacology and Toxicology 2021Quote: Microarray analyses were conducted using the human Clariom D Affymetrix platform following the manufacturer’s instructions (902915, Thermo Fisher Scientific). Briefly ...
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bioRxiv - Molecular Biology 2020Quote: ... the coding sequence of the current human textitTRBP reference (RefSeq NM_134323) was amplified and subcloned into the pcDNA3-cMyc vector (Invitrogen) at the BamHI and XhoI sites ...
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bioRxiv - Cancer Biology 2020Quote: CAFs were isolated from human colon carcinoma resection specimens following 2.4U/ml dispase + 100U/ml collagenase type II (both from Invitrogen); disaggregation of tumour fragments and digestion for 2h t 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... and hybridized to the Affymetrix Human Gene 1.0 ST Arrays using the Gene Chip WT Terminal Labeling and Hybridization Kit (Affymetrix). GeneChip fluidics station 450 was used for processing of the arrays and fluorescent signals were detected with the GeneChip scanner 3000-7 G ...
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bioRxiv - Bioengineering 2021Quote: ... media was changed to the following formulation of EC medium: human endothelial serum-free medium (hESFM, Thermo Fisher 1111044) supplemented with 1% platelet-poor plasma-derived serum (PDS ...
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bioRxiv - Immunology 2020Quote: Human PBMCs were incubated with MitoSOX Red (5μM) and Mitotracker Green (1 μM) (both from Molecular Probes, Life Technologies) in PBS during 10 minutes at 37°C before flow cytometry extra-cellular staining.
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bioRxiv - Immunology 2020Quote: Human PBMCs were incubated with MitoSOX Red (5μM) and Mitotracker Green (1 μM) (both from Molecular Probes, Life Technologies) in PBS during 10 minutes at 37°C before flow cytometry extra-cellular staining.
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bioRxiv - Immunology 2021Quote: ... T lymphocytes were purified using the Pan T cell isolation kit (Miltenyi Biotech) and activated with Dynabeads Human T-Activator CD3/CD28 (1:1 beads:cell) (ThermoFisher) in X-vivo 15 medium (Lonza ...
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bioRxiv - Immunology 2020Quote: ... Primary human CD8+ T cells were cultured for up to 24 days in ATCC-modified RPMI (Thermo Fisher, #A1049101) supplemented with 10% FBS ...
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bioRxiv - Immunology 2021Quote: ... The human myelomonocytic leukemia cell line THP-1 was propagated in RPMI 1640 medium (GIBCO® Life Technologies™) supplemented with 10% fetal calf serum (FCS ...
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bioRxiv - Genomics 2019Quote: ... Activation was performed by addition of Dynabeads Human T-Activator CD3/CD28 (25 ul/0.5 million cells; Life Technologies), along with IL-2 (30 U/ml ...
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bioRxiv - Cancer Biology 2019Quote: ... H1299 (human non-small cell lung carcinoma, ATCC CRL-5803) and HMGA2 KO cells were cultured in RPMI (GIBCO) supplemented with 10% FBS (Gibco ...
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bioRxiv - Biochemistry 2019Quote: ... The THP-1 (human monocytic cells) cells were grown on Roswell Park Memorial Institute (RPMI) medium (Gibco, CA, USA) supplemented with 10% FBS and 0.05 mM β-mercaptoethanol (Sigma-Aldrich ...
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bioRxiv - Genomics 2021Quote: 40-80 ng of each probe (labeled with biotin or Dig) and 1µg of unlabeled human COT1-DNA (Invitrogen) were dissolved in 4µl of hybridization buffer (10% dextran sulfate ...
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bioRxiv - Genomics 2021Quote: Human chronic myelogenous leukemia K562 cells (ATCC CCL-243) were cultured in RPMI 1640 Medium with GlutaMAX (Gibco 61870127), supplemented with 10% fetal calf serum (FCS ...
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bioRxiv - Genetics 2020Quote: ... HeLa and Human foreskin fibroblast (HFF) cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% FBS (Gibco). N2a cells were maintained in 45% DMEM and 45% Opti-MEM supplemented with 10% FBS (Gibco) ...
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bioRxiv - Microbiology 2020Quote: The AGS human gastric epithelial cell line (ATCC CRL-1739) was maintained in DMEM/F-12 (Thermo Fisher Scientific) containing 10% (v/v ...
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bioRxiv - Cell Biology 2021Quote: ... and Human pancreatic epithelial cells (hTERT-HPNE, ATCC, CRL-4023) were cultured in pyruvate-free DMEM (Gibco, 11965-092) supplemented with 10% FBS and P/S ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA from native pig and human lung tissue (fresh snap-frozen) was isolated using the PureLink Mini Kit (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2021Quote: Synthetic genes encoding for human merlin FERM (22-312) and radixin FERM (5-295) domains were commercially synthesized (ThermoFisher) and cloned into pETM33 using NcoI and EcoRI restriction sites ...
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bioRxiv - Biochemistry 2021Quote: ... The peptide–mRNA fusions were then incubated with human and yeast DUBm-immobilized on Dynabeads M-280 streptavidin (Invitrogen) for 30 min at 37 °C ...
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bioRxiv - Biochemistry 2021Quote: ... Human ASGR1 and its mutants (Q240A/W244A and Q240A/W244A/E253A) and human ASGR2 were sub-cloned into pcDNA 3.1 vector (Invitrogen) containing a C-terminal Flag tag (ASGR1 ...
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bioRxiv - Synthetic Biology 2021Quote: Flp-In™ T-REx™ Human Embryonic Kidney (HEK) 293 cells were purchased from Thermo Scientific (Catlog# R78007). Cells were cultured in a humidity-controlled incubator under standard culture conditions (37°C with 5% CO2 ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with 0.05 mg/mL bovine pituitary extract and 5 ng/mL human recombinant epidermal growth factor (Gibco, USA). Cells were maintained in a humidified incubator at 37°C with 5% CO2 level ...
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bioRxiv - Developmental Biology 2021Quote: A human episomal iPSC line derived from CD34+ cord blood was used in this study (A18945, ThermoFisher Scientific; [58]). This cell line has already been validated in its ability to generate light-sensitive 3D retinal organoids ...
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bioRxiv - Immunology 2021Quote: ... This was followed by addition of alkaline phosphatase-conjugated goat anti-human F(ab’)2 antibody (Thermo Fisher Scientific) Inc. ...
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bioRxiv - Immunology 2021Quote: Cells from the human cancer cell line Colo205 were cultivated in complete RPMI-1640 medium (Thermo Fisher Scientific, USA) supplemented with 2 mM L-glutamine ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Caco-2 human IECs (ATCC #HTB-37) were grown in Dulbecco’s Modified Eagle Medium (DMEM; #11965, Gibco-ThermoFisher Scientific) supplemented with 10% (v/v ...