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Citations for Thermo Fisher :
6251 - 6300 of 10000+ citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Human foreskin fibroblast (HFF) cells were grown and maintained in DMEM containing 4.5g/L glucose (Gibco 11965-092), supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Molecular Biology 2021Quote: HEK293F is a female human embryonic kidney cell line transformed and adapted to grow in suspension (Life Technologies). HEK293F cells were grown in 293FreeStyle expression medium (Life Technologies) ...
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bioRxiv - Cell Biology 2021Quote: ... Alpha-actinin-1-M-sstFRET containing the sstFRET module inserted between 300aa and 301aa within the first spectrin repeat of human alpha-actinin-1 (P12814.2) was in the neomyocin selectable pcDNA3.1 expression vector (Invitrogen/ThermoFisher). Actinin-C-sstFRET containing the sstFRET module added to the C-terminus of human alpha-actinin-1 (P12814.2 ...
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bioRxiv - Immunology 2022Quote: ... Human Raji (Burkitt’s lymphoma) and SKOV3 (ovarian carcinoma) were cultured in complete RMPI (Roswell Park Memorial Institute, Gibco) and DMEM (Dulbecco’s Modified Eagle’s Medium ...
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bioRxiv - Immunology 2022Quote: ... podocytes were treated with patient sera in the presence of a control antibody (human IgG, Invitrogen #02-7102) or anti uPAR antibody (2G10 ...
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bioRxiv - Biophysics 2022Quote: ... Poly-L-lysine coated chambers were then incubated with anti-human CD3 antibody (HIT3a clone, Thermo Fisher Scientific) in PBS at a concentration of 10 µg/mL for 2 hours at 37°C or overnight at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... cells and MDA-MB-231 human breast cancer cells were obtained from ATCC and cultured in Dulbecco’s Modified Eagle’s Medium (DMEM; Gibco) with reduced glucose (1 g/L) ...
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bioRxiv - Cancer Biology 2022Quote: ... T cells were isolated from PBMCs using the Dynabeads® Untouched Human T Cells Kit (Invitrogen, Karlsruhe, Germany) following the manufacturer’s protocol.
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bioRxiv - Biochemistry 2022Quote: ... labeled with Alexa Fluor 488 from the Zenon™ Human IgG Labeling kit (Molecular Probes, Eugene, OR, USA) and 15 μg/mL of 7-amino-actinomycin D (7-AAD ...
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bioRxiv - Biophysics 2022Quote: ... designated as ‘Cav1.2(ΔC)’) construct was obtained from a full-length human Cav1.2 α1 (Uniprot Q13936-20) template in pcDNA3.1 (Invitrogen) using a Gibson Assembly kit (NEB) ...
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bioRxiv - Biochemistry 2022Quote: ... Histidine rich glycoprotein (HRG) was purified from human plasma with a cobalt-loaded resin (Thermo Scientific, Waltham, America) using IMAC-based enrichment (56) ...
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bioRxiv - Cancer Biology 2022Quote: ... 50 μl each) using the Immune Monitoring 65-Plex Human ProcartaPlex Panel (ThermoFisher, Catalog number: EPX650-16500-901), as per manufacturer instructions ...
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bioRxiv - Cell Biology 2022Quote: ... and aged (>60 years) human primary keratinocytes (HPK) were isolated with trypsin (Gibco, Life Technologies, Carlsbad, CA, USA) and dispase (Dispase II ...
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bioRxiv - Cell Biology 2022Quote: ... and aged (>60 years) human primary keratinocytes (HPK) were isolated with trypsin (Gibco, Life Technologies, Carlsbad, CA, USA) and dispase (Dispase II ...
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bioRxiv - Cell Biology 2022Quote: Human acute monocytic leukemia THP-1 (ATCC, TIB-202™) cells were cultured in RPMI-1640 medium (Gibco) supplemented with heat-inactivated 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2022Quote: ... Human IFNg and IL-13 cytokine secretion was measured by ELISA using eBioscience Ready-SET-Go! (now ThermoFisher) ELISA kits according to the manufacturer’s protocol.
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bioRxiv - Immunology 2022Quote: ... Gene expression levels were obtained using Human Clariom S arrays on GeneTitan MC Instrument International (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2022Quote: ... and the human epithelial cell line A549 (ATCC No. CCL-185) were cultivated in Dulbecco’s modified Eagle’s medium (DMEM; Gibco BRL Life Technologies ...
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bioRxiv - Microbiology 2022Quote: ... falciparum was maintained at 4% haematocrit in human RBCs in AlbumaxII media (RPMI-1640, 25 mM HEPES (Gibco), 367 μM hypoxanthine (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2022Quote: ... human embryonic kidney (HEK) 293T and Vero cells (ATCC) were maintained with Dulbecco’s Modified Eagle’s medium (DMEM) (Gibco), supplemented with 10% fetal calf sera (FCS ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... cells stably expressing human M4 mAChR or mutant constructs were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Invitrogen) containing 5% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2022Quote: Human BDNF-biotin (50 nM) was incubated with 50 nM Qdot-605 Streptavidin conjugate (Thermo Fisher Scientific; Q10101MP) on ice for 1 h in Neurobasal medium to facilitate the biotin-streptavidin interaction ...
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bioRxiv - Molecular Biology 2023Quote: Human retinal epithelial cells (hTERT-RPE1, ATCC) were grown in 10% (v/v) FBS (Atlas) / DMEM (Thermo Scientific). Media was supplemented with 1 mM L-glutamine (Gibco ...
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bioRxiv - Microbiology 2023Quote: Human primary fibroblasts (HFFs, ATCC® CCL-171™) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2022Quote: ... and human cervical cancer cells (HeLa) were maintained in Dulbecco’s modified Eagle’s (DMEM, 11965092, GIBCO, Carlsbad, CA, USA) medium ...
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bioRxiv - Immunology 2022Quote: ... and CD4+ and CD8+ T cells were further isolated with Dynabeads Human CD4+ or CD8+ Isolation Kits (ThermoFisher), following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... the membrane was incubated with primary antibody against aSyn (Monoclonal anti-human mouse α-synuclein Syn211 Invitrogen™), against A11 oligomers (Polyclonal Rabbit Anti-amyloid oligomer A11 AB9234 Millipore™ ...
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bioRxiv - Neuroscience 2022Quote: ... hiPS cells were maintained in 6-well plates coated with recombinant human vitronectin (VTN-N, Life Technologies, A14700) in Essential 8 medium (Life technologies ...
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bioRxiv - Neuroscience 2022Quote: ... cells were changed to EC medium consisting of human endothelial serum-free media (hESFM; Thermo Fisher Scientific #11111044), 0.25X B27 supplement (Fisher Scientific #17-504-044) ...
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bioRxiv - Microbiology 2022Quote: ... mouse fibroblast cell lines and HEK293T human cell lines (ATCC CRL-3216) were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Gibco) with 10% fetal calf serum (FBS ...
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bioRxiv - Cell Biology 2022Quote: Two separate human ASAH1-siRNAs (50 nM) (Dharmacon,) were transfected into 621-101 cells using Lipofectamine RNAiMAX (Invitrogen) according to the manufacturer’s protocols ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Total RNA was extracted from mouse skin tissue or mouse/human EpSCs using Trizol reagent (Invitrogen, Massachusetts, USA), and used as a template for cDNA synthesize using HiScript II reverse transcriptase (Vazyme Biotech ...
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bioRxiv - Biophysics 2024Quote: Human embryonic kidney (HEK) 293 cell lines were maintained in T-25 flasks using TrypLE Express Enzyme (Gibco) and DMEM with L-Glutamine ...
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bioRxiv - Physiology 2024Quote: ... Total RNA Microarray analysis was performed with the Affymetrix GeneChip Human Transcriptome Array 2.0 (Thermo Fisher Scientific, MA). Metabolomic analysis was performed by Metabolon (Durham ...
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bioRxiv - Neuroscience 2024Quote: Human fibroblast cells were reprogrammed using the Sendai virus-based CytoTune iPS 2.0 kit (CytoTune 2.0, Invitrogen, A16517). Fibroblasts were plated in six-well plates two days before transduction to achieve a density of 2 × 105 to 3 × 105 cells per well ...
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bioRxiv - Microbiology 2024Quote: ... and then treated with Alexa Fluor 488-conjugated goat anti-human IgG (catalogue no. A11013; Thermo Fisher Scientific) at a concentration of 2 μg/ml in DMEM with 2% FBS for 30 minutes (min ...
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bioRxiv - Microbiology 2024Quote: Human bladder epithelial cell line 5637 (RRID: CVCL_0126) were cultured and maintained in RPMI medium (Gibco; ThermoFisher Scientific) supplemented with 10% heat inactivated FBS (Sigma ...
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bioRxiv - Microbiology 2024Quote: Human bladder epithelial cell line 5637 (RRID: CVCL_0126) were cultured and maintained in RPMI medium (Gibco; ThermoFisher Scientific) supplemented with 10% heat inactivated FBS (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... Combined libraries were dried down with universal blockers and human cot DNA in a Savant SpeedVac (ThermoFisher Scientific) vacuum concentrator ...
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bioRxiv - Cancer Biology 2023Quote: ... Adherent human glioblastoma cell lines and patient-derived cell cultures were passaged using Trypsin-EDTA (0.25%, Gibco, #25200056). For DRUG-seq ...
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bioRxiv - Microbiology 2023Quote: ... while uninfected bovine macrophages (BoMac)64 and human embryonic kidney cells (HEK293FT) were grown in DMEM medium (Gibco). HAP1 cells ...
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bioRxiv - Neuroscience 2024Quote: A synthetic DNA geneblock for the human PAC1R-null receptor sequence (PAC1R) was designed and ordered (Life Technologies) based on the NCBI protein data bank entry “NP_001109.2” ...
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bioRxiv - Molecular Biology 2024Quote: Human Embryonic Kidney 293 (HEK) cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Life Technologies-Invitrogen, USA), supplemented with 10% Fetal Bovine Serum Gold (FBS ...
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bioRxiv - Molecular Biology 2024Quote: The cDNA sequence of human Sph1 was subcloned from the pcDNA3.1/V5-His-TOPO expression vector (Invitrogen, USA) 62 into the Venus-BiFC plasmids previously described 37 ...
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bioRxiv - Molecular Biology 2024Quote: Human Embryonic Kidney 293 (HEK) cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Life Technologies-Invitrogen, USA), supplemented with 10% Fetal Bovine Serum Gold (FBS ...
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bioRxiv - Microbiology 2024Quote: ... A549 (Human Lung carcinoma cell line) and HeLa-hACE2 were cultured in DMEM (Thermo Fisher Scientific, Waltham, USA) containing 10% fetal bovine serum in a 5% CO2 incubator at 37℃ ...
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bioRxiv - Neuroscience 2024Quote: Human CSF2RB WT (exons 11 to 14) and A455D mutant donor targeting sequences were cloned into pDONR221 (ThermoFisher) with the following layout (Supplementary Figure S1) ...
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bioRxiv - Cancer Biology 2023Quote: ... dissociated PDTOs were incubated with 150 μg/mL of mouse anti-human HLA-ABC (clone W6/32, ThermoFisher) to block MHC-I and 30 μg/mL mouse anti-human HLA-DR/DP/DQ (clone Tu39 ...
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bioRxiv - Immunology 2023Quote: ... followed by the transmembrane domain (aa 1636– 1671) of human PTPRZ1 (gene synthesized by GeneArt, Thermo Fisher Scientific). Resistance to puromycin (InvivoGen ...
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bioRxiv - Neuroscience 2023Quote: ... washed 3 x 5 min with PBS and incubated with Alexa Fluor-488 goat anti human secondary (Invitrogen) for 1 hour at room temperature ...