Labshake search
Citations for Thermo Fisher :
6301 - 6350 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2024Quote: ... 1% N-2-hydroxyethylpiperazine-N’-2-ethanesulfonic acid (HEPES, Gibco, #15630080) and 1% MEM nonessential amino acids (MEM-NEAA ...
-
bioRxiv - Microbiology 2023Quote: ... 25 mM N-2-hydroxyethylpiperazine-N’-2-ethanesulfonic acid (HEPES, Gibco), 2 mM L-glutamine (Gibco) ...
-
bioRxiv - Biochemistry 2022Quote: ... and 2 mM GlutaMax supplemented with 2% (wt/vol) B27 (GIBCO) and 10% FBS ...
-
bioRxiv - Cancer Biology 2023Quote: ... in a 2:2:1 molar ratio using lipofectamine 3000 (Invitrogen) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2023Quote: ... 25 mM N-2-hydroxyethylpiperazine-N’-2-ethanesulfonic acid (HEPES, Gibco), 2 mM L-glutamine (Gibco) ...
-
bioRxiv - Cell Biology 2021Quote: ... and 2-NDBG [2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose] (N13195) were purchased from Invitrogen. Recombinant murine SCF (250-03) ...
-
bioRxiv - Microbiology 2022Quote: ... Cat #: 14793, 1:250 dilution) (mouse anti-PDI, Thermo Fisher, Cat #: MA3-019, 1:200 dilution) (mouse anti-Spike S2, Thermo Fisher, Cat #: MA5-35946, 1: 500 dilution) (rabbit anti-TGN46 ...
-
bioRxiv - Cancer Biology 2021Quote: The BCR-ABL-positive B-cell leukemia cell line BV173 (DSMZ, Braunschweig, Germany) was cultivated in RPMI1640 Supplemented with 10% FCS and 1% penicillin/streptomycin (Gibco).
-
bioRxiv - Cell Biology 2020Quote: ... the cell viability was determined using a micro plate reader (Thermo Scientific Multiskan FC, Thermo Fisher Scientific Oy, Ratstie, Finland) according to the manufacture’s protocol of CCK-8 ...
-
bioRxiv - Cell Biology 2020Quote: HELA cells were plated in a 12-well plate in DMEM+ 10%FCS and transiently transfected using Lipofectamine3000 (Thermofisher, L3000001). Each transfection consisted of 250 ng expression plasmid (pcDNA3-control (Addgene ...
-
Optimized immunoglobulin knock-ins using Cas9 reveal peritoneal B cell lineage relationships in vivobioRxiv - Immunology 2021Quote: ... Intestinal pieces were then placed into 10 mL RPMI 20% FCS with 0.5 mg/mL Collagenase Type I (Gibco #17100017) and 0.1 mg/mL DNase I (Sigma #DN25 ...
-
bioRxiv - Microbiology 2022Quote: Unspecific Fc binding was blocked for 15 minutes on ice with unconjugated anti-CD16/CD32 (clone 93; Thermo-Fisher Scientific). Cells were subsequently fluorescently labeled for 30 minutes on ice with the following antibodies and reagents ...
-
bioRxiv - Immunology 2022Quote: ... Dead cells were excluded using ThermoFisher LIVE/DEAD Fixable Aqua Dead Cell Stain and binding to Fc receptors was blocked using CD16/CD32 (clone 93, eBioscience/ThermoFisher). Absolute cell numbers were calculated using counting beads (123count eBeads ...
-
bioRxiv - Immunology 2021Quote: ... and the ELISPOT plate was incubated for 4 hours at 4°C with biotinylated goat anti-human IgG Fc (Invitrogen), followed by incubation for 1 hour at room temperature with horseradish peroxidase (HRP)–conjugated avidin (Vector Laboratories) ...
-
bioRxiv - Immunology 2021Quote: ... Induced yeast libraries were stained for antigen binding (RBD-Fc APC tetramers) and scFv expression (chicken anti-c-myc IgY; Invitrogen). Following two washes in PBSF (1x PBS ...
-
bioRxiv - Immunology 2019Quote: ... ATCC CRL-3216) cells were maintained in DMEM-Glutamax supplemented with 10% heat-inactivated fetal calf serum (FCS, ThermoFisher Scientific) and Penicillin-Streptomycin (PS ...
-
bioRxiv - Developmental Biology 2019Quote: ... The remaining tissue was let to sediment and the supernatant was collected in a tube containing the same volume of 10% FCS (Gibco)-HBSS+/+ and kept on ice ...
-
bioRxiv - Cancer Biology 2020Quote: ... grown in DMEM supplemented with 10% FCS and neurospheres (RNS, enriched in cancer stem cells) grown in DMEM/Ham’s F12 GlutaMAX (Life Technologies) supplemented with B27 and N2 additives (Invitrogen ...
-
bioRxiv - Microbiology 2019Quote: ... cell number enumerated with a hemocytometer and resuspended in 0.5 ml PBS supplemented with fetal calf serum (FCS, ThermoFisher Scientific) to a final concentration of 1 × 103 cells/ml in each well of a 24 well plate ...
-
bioRxiv - Cell Biology 2019Quote: HEK293 cells were cultured in DMEM (Biowhitaker) supplemented with 10% fetal calf serum (FCS) (v/v) and 1% penicillin/streptomycin (GIBCO). Inducible cell lines were selected on 5 μg/ml blasticidin (Invitrogen ...
-
bioRxiv - Biochemistry 2020Quote: ... were cultured and maintained at 37 °C in a humidified atmosphere of 5 % CO2 in air in Dulbecco’s Modified Eagle’s Medium (DMEM) + GlutMAXTM-1 containing 10 % Fetal calf serum (FCS) and 1% Penicillin-Streptomycin (10 000 U/ml) (Gibco Life Technologies ...
-
bioRxiv - Microbiology 2021Quote: ... which is composed of two ACE2 ectodomains linked to the Fc portion of the human IgG (Anand et al., 2020).Alexa Fluor-647-conjugated goat anti-human Abs (Invitrogen) were used as secondary antibodies to detect ACE2-Fc and plasma binding in flow cytometry experiments.
-
bioRxiv - Cancer Biology 2021Quote: ... for the last 30 min of the drug treatment in L-methionine-depleted medium containing 5% dialyzed fetal calf serum (FCS; Gibco). Harvested cells were washed with PBS ...
-
bioRxiv - Cancer Biology 2020Quote: ... BALB/c-derived A20 B lymphoma cell line was obtained from the American Type Culture Collection (Rockville, MD) and maintained in RPMI 1640 supplemented with 10% FCS (GIBCO). For in vivo experiments A20 cell lines were injected i.v ...
-
bioRxiv - Cell Biology 2021Quote: ... washed twice with ice cold Cytoperm reagent and re-suspended in 1% fetal calf serum (FCS) in PBS (Invitrogen, 14190). Cells were subsequently analyzed on a CYAN flow cytometer (Beckmann Coulter).
-
bioRxiv - Microbiology 2020Quote: ... Cells were maintained at 37°C in the presence of 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% heat-inactivated fetal calf serum (FCS) and 1% penicillin–streptomycin (fresh medium) (all from Invitrogen). Cells were used between 10 and 35 passages ...
-
bioRxiv - Microbiology 2020Quote: ... supplemented with 5% foetal calf serum (FCS) and antibiotics (100 units/mL penicillin, 100 mg/mL streptomycin, GIBCO, Life Technologies). KC cells (A Culicoides insect derived cell line ...
-
bioRxiv - Microbiology 2020Quote: ... supplemented with 5% foetal calf serum (FCS) and antibiotics (100 units/mL penicillin, 100 mg/mL streptomycin, GIBCO, Life Technologies). KC cells (A Culicoides insect derived cell line ...
-
bioRxiv - Neuroscience 2020Quote: ... After washing chase medium (DMEM containing 10% FCS, Glutamax, P/S, 1 mg/mL methionine and 1 mg/mL cysteine (Gibco)) was added to cells and incubated for different time points ...
-
bioRxiv - Immunology 2021Quote: ... splenic NK cells were isolated and suspended in NK cell medium (phenol-red free RPMI 1640 containing 10% FCS, non-essential amino acids, L glutamine and sodium pyruvate, all from Gibco). The indicated target cells were labelled with 15μM Calcein-AM (Life Technologies ...
-
bioRxiv - Microbiology 2019Quote: ... The overnight inoculum was diluted 1:33 in infection medium (DMEM + 10% FCS + HEPES (Life Technologies, cat. number: 15630-056)) to obtain OD600=1 after 3 h of incubation to give 1.5 × 109 bacteria/ml ...
-
bioRxiv - Microbiology 2021Quote: ... Membranes were washed three times for 5 min and incubated with 1:3000 dilution of horseradish peroxidase-conjugated Goat anti-mouse IgG Fc secondary antibody (Invitrogen) for 1 h ...
-
bioRxiv - Microbiology 2020Quote: ... University of Bristol) were grown in DMEM supplemented with 8% FCS and 8% (v/v) tryptose phosphate broth (Life technologies). Penicillin and streptomycin (90 IU/ml ...
-
bioRxiv - Developmental Biology 2022Quote: ... These zona-free eggs were incubated with 200 nM Fc-fusion proteins in Biggers-Whitten-Whittingham (BWW) medium for 1 h and then stained with goat anti-human IgG Fc antibody DyLight 488 (Invitrogen) at a dilution of 1:50 for 1 h at 37 °C ...
-
bioRxiv - Microbiology 2022Quote: ... Antibody binding was detected using goat anti-Human IgG Fc Secondary Antibody conjugated to horse radish peroxidase (Thermo Fisher Scientific), diluted 1:5000 in blocking solution and incubated for 1 hr at RT ...
-
bioRxiv - Microbiology 2022Quote: ... and then incubated with a 1:10000 dilution (in PBST with 1% BSA) of goat anti-human Fc HRP-conjugated antibody (Invitrogen) at room temperature for 1 hour ...
-
bioRxiv - Immunology 2022Quote: ... RPMI-1640 medium supplemented with 10% heat inactivated FCS and final concentrations of 100 U/mL penicillin and 100 μg/mL steptomycin and 2mM L-Glutamine (Gibco). Cells were incubated with OVA peptide (323-339 ...
-
bioRxiv - Cancer Biology 2022Quote: ... Cells were cultured in Dulbecco’s modified eagle medium (DMEM) containing 10% fetal calf serum (FCS) (Thermo Fisher Scientific, Hamburg, Germany), 100 IU/ml penicillin and 100 μg/ml streptomycin (Life Technologies ...
-
bioRxiv - Immunology 2020Quote: ... and cells were stained with Fixable Viability Dye (eBioscience) and primary antibodies in the presence of Fc block in flow buffer (DPBS [Gibco] supplemented with 2% FCS ...
-
bioRxiv - Immunology 2020Quote: ... Biopsies were washed in PBS and transferred into wells containing RPMI-1640 + 10% FCS + 20 μg/mL G418 (Thermo Fisher) + 20 U/mL Pen/Strep and cultured for 24 hours.
-
bioRxiv - Molecular Biology 2021Quote: ... and S309 and VHH72-Fc or variants thereof was detected with an AF633 conjugated goat anti-human IgG antibody (Invitrogen). Binding of monomeric VHHs to SARS-CoV-1 or SARS-CoV-2 S was detected with a mouse anti-HisTag antibody (AbD Serotec ...
-
bioRxiv - Molecular Biology 2020Quote: ... the receptor Fc-tagged ectodomains present in the conditioned media were captured on protein A-coated 384-plates (Thermo Scientific), and stored at 4 °C until use ...
-
bioRxiv - Cell Biology 2021Quote: ... BSF RNAi cell lines were cultured in HMI-9 medium 47 supplemented with 10% (v/v) low-tetracycline foetal calf serum (FCS; Gibco) at 37 °C in 5% C02 and maintained in 5 µg.mL-1 Hygromycin (InvivoGen ...
-
bioRxiv - Cancer Biology 2020Quote: ... The CH12.F3 mouse B lymphocyte cell line (a generous gift from T. Honjo (Kyoto Univ.)) was cultured in RPMI/10% FCS/5% NCTC-109 (Invitrogen)/1X Penn-Strep/50μM αMeOH ...
-
bioRxiv - Cancer Biology 2019Quote: ... All media were supplemented with 10% Foetal Calf Serum (FCS) (First Link, Birmingham, UK) as well as 100 units/mL penicillin and 100 μg/mL streptomycin (Gibco™ through Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2020Quote: Up to 5×106 isolated cells were incubated for 30 min at 4 °C with anti-CD16/CD32 (Fc receptor) clone 93 (Invitrogen) to block non-specific binding and with fixable viability dye eFluor455UV (eBioscience ...
-
bioRxiv - Biochemistry 2021Quote: ... The slide was washed thrice with PBS-T for 1 minute and further incubated with goat anti-human IgG (Fc)-Cy5 (Invitrogen) antibodies at 1:2500 dilution ...
-
bioRxiv - Immunology 2022Quote: ... Blots were imaged using the LiCor Odyssey® Fc system after treatment with ECL Western Blotting-Substrat (Thermo Fisher, 32209).
-
bioRxiv - Immunology 2022Quote: ... femurs and tibia of mice were flushed with complete medium (RPMI-1640 and 10% heat inactivated fetal calf serum (FCS) (Gibco). Single cell suspensions were incubated with 5 mL of red blood cell (RBC ...
-
bioRxiv - Immunology 2022Quote: Surface antibody staining was performed in PBS containing 5% FCS and 1mM EDTA and using a Fixable Aqua Dead Cell (Invitrogen) to determine viability ...