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Citations for Thermo Fisher :
6101 - 6150 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... the peptides from each sample were reacted for 1 h with the appropriate tandem mass tag (TMT) isobaric reagent (Thermo Fisher) in 40% (v/v ...
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bioRxiv - Molecular Biology 2022Quote: Insect or bacterially expressed proteins were purified by His-tag purification using hiTRAP FF columns (GE) or HisPur Resin (Thermo Scientific), followed by His tag removal by Tobacco Etch Virus (TEV ...
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bioRxiv - Biochemistry 2022Quote: ... was cloned in frame with a N-terminal GFP tag and intervening short peptide linker (GSGEGRG) into the pcDNA5/FRT/TO bacterial expression vector (Invitrogen; V652020). A C-terminal histidine tag (Hisx6 ...
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bioRxiv - Microbiology 2022Quote: ... The recombinant proteins containing a hexa-histidine tag was purified by passing through the nickel-NTA-affinity Column (HisPurTM Ni-NTA Spin Columns, Thermo Fisher) following supplier’s instruction ...
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bioRxiv - Cancer Biology 2022Quote: 3T3 cells were expanded after selection to create a stable line and collected for HA-tag Co-IP analysis (Thermo Scientific™ Pierce™ HA-Tag IP/Co-IP Kit ...
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bioRxiv - Immunology 2020Quote: ... The lysates were then resolved by SDS-PAGE and immunoblotted with indicated antibodies (Wapl and V5-tag antibodies were purchased from Thermo Fisher Scientific #PA5-38024 and #R960-25 ...
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bioRxiv - Molecular Biology 2020Quote: Sample aliquots comprising 25 μg of peptides were labelled with isobaric tandem mass tags (TMT 10-plex, Thermo Fisher Scientific, 90110) as described previously31 ...
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bioRxiv - Immunology 2021Quote: ... Extracellular fragment of human CD4 comprising domains 1-4 of human CD4 and a C-terminal His6-tag was expressed in Expi293 cells according to the manufactureŕs protocol (Thermo Fisher Scientific). Cell supernatant was harvested by centrifugation 4 days after transfection ...
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bioRxiv - Immunology 2021Quote: ... GenBank: QHD43416.1) with an N-terminal IgKappa signal sequence (METDTLLLWVLLLWVPGSTG) and C-terminal TwinStrep- and 3xFLAG-tags (GeneArt, ThermoFisher, Regensburg, Germany) into a pcDNA3.4(+ ...
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bioRxiv - Biophysics 2021Quote: ... and the cleaved protein was separated from the affinity tags and the protease by passing it through the POROS MC 20 Ni2+ column (Applied Biosystems) in tandem with a 20-mL amylose column (New England Biolabs ...
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bioRxiv - Neuroscience 2022Quote: ... labelled with Tandem Mass Tag (TMTpro) sixteen plex reagents according to the manufacturer’s protocol (Thermo Fisher Scientific, Loughborough, LE11 5RG, UK) and the labelled samples pooled.
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bioRxiv - Immunology 2019Quote: ... Lysates and purified products were analyzed by acrylamide gel electrophoresis followed by standard coomassie blue staining and western blot analysis using a rabbit anti-GST Tag polyclonal antibody (CAB4169, Thermo Fisher).
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bioRxiv - Microbiology 2020Quote: ... The resulting blots were probed with a sdAb as primary antibody and an HRP-linked 6x-His Tag antibody (Thermofisher, HIS.H8) as the secondary antibody ...
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bioRxiv - Neuroscience 2020Quote: ... This resulted in plasmid pBS-FZ2.FLAG which was used to amplify the dFZ2 ORF with the FLAG tag for cloning into the Gateway entry vector pENTR-D-TOPO (Thermo Fisher). This step eliminated most of the dFZ2 5’ and 3’ UTRs while maintaining the presumptive Kozak sequence adjacent to the start codon ...
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bioRxiv - Microbiology 2020Quote: ... The synthesized coding DNAs with a 6-His-tag expressed at the C-terminus were cloned into the pPinkα-HC expression vector (Thermofisher, US) using XhoI/KpnI restriction sites ...
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bioRxiv - Neuroscience 2019Quote: ... TAG concentrations were normalized to the total protein content as determined by a Micro BCA protein assay kit (ThermoFisher, Cat # 23235). Each assay was done in triplicate and the average TAG level (μg TAG/ μg protein ...
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bioRxiv - Physiology 2020Quote: ... 50 μg of peptides from each sample were labelled with 5 μl of Tandem Mass Tag 10-plex (TMT, Thermo Scientific) reagent (0.02mg/μl ...
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bioRxiv - Cell Biology 2020Quote: Recombinant 6XHis-tagged TbVps24 (N-terminus tag) was cloned using the pTrcHis TOPO TA expression system (Thermo Fisher Scientific, Carlsbad, CA) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... All constructs have a C-terminal 3XFLAG epitope tag and were cloned into the pcDNA4/TO vector backbone (Thermo Fisher, #V102020) using restriction sites at EcoRI/XhoI.
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bioRxiv - Immunology 2021Quote: ... Two initial rounds of magnetic-activated cell sorting were performed on the library using decreasing amounts of Ray Biotech RBD-coated His-Tag Dynabeads (Thermo Fisher). The resulting enriched library pool was subsequently sorted using a Sony SH800 Cell Sorter ...
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bioRxiv - Immunology 2020Quote: ... Gene expression and antigen tag libraries were analyzed on the Agilent D1000 Tapestation and quantified using the Qubit HS dsDNA fluorometric quantitation kit (Thermo Scientific).
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bioRxiv - Neuroscience 2020Quote: ... 5 μL the corresponding amine-based tandem mass tag (TMT) 10-plex reagents (10 μg/μL) (Thermo Fisher Scientific, Cat # 90406) was added and incubated for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... in the Department of Pathology at the University of Michigan using mass spectrometry (MS)-based tandem mass tag (TMT) analysis (ThermoFisher Scientific). The complete details for the experimental conditions ...
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bioRxiv - Cell Biology 2021Quote: ... and an equal amount of each labelled with Tandem Mass Tag (TMT) ten plex reagents according to the manufacturer’s protocol (Thermo Fisher Scientific) and the labelled samples pooled.
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bioRxiv - Cell Biology 2021Quote: ... overnight) and labelled with Tandem Mass Tag (TMT) ten plex reagents according to the manufacturer’s protocol (Thermo Fisher Scientific, Loughborough, UK), and the labelled samples pooled.
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bioRxiv - Cancer Biology 2022Quote: ... p41Plk3 mammalian expression vector for protein expression and purification was generated by performing an LR recombination reaction between the pENTR/D-TOPO entry clone and a Gateway destination vector pcDNA-DEST40 containing His-tag (Life Technologies). ShPlk3s (shRNA-A ...
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bioRxiv - Biochemistry 2022Quote: ... and Pcdh15 EC 1-2 with 6x His and GFP tag at the C-terminus were transfected and expressed in the ExpiCHO suspension cell system (A29129 ThermoFisher Scientific) using the standard prescribed protocol ...
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bioRxiv - Cell Biology 2022Quote: ... 37 °C overnight) and labelled with Tandem Mass Tag (TMT) eleven-Plex reagents according to the manufacturer’s protocol (Thermo Fisher Scientific) before labelled samples were pooled.
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bioRxiv - Cell Biology 2022Quote: ... labelled with Tandem Mass Tag (TMT) eleven plex reagents according to the manufacturer’s protocol (Thermo Fisher Scientific, Loughborough, LE11 5RG, UK) and the labelled samples pooled ...
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bioRxiv - Plant Biology 2022Quote: ... The potato ST-LS1 intron-containing version of S65TGFP used as C-terminal tag to avoid leaking expression of the fluorophore in agrobacteria (Liu et al., 2019b) was synthesized by Life Technologies. For EXO70H4 ...
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bioRxiv - Neuroscience 2023Quote: Peptides were reconstituted in 100ul of 100mM triethyl ammonium bicarbonate (TEAB) and labeling performed as previously described75,76 using TMTPro isobaric tags (Thermofisher Scientific, A44520). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: ... FLAG-tag MSCV-MSI2-IRES-GFP or FLAG-tag MSCV-MSI2HOXA9-IRES-GFP infected K562 cells were labeled with 100nM Mitotracker Deep Red FM (Thermo Fisher) at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... the PCR products were directly labeled with Alexa Fluor 555 dye and purified using the FISH Tag DNA Orange Kit (ThermoFisher Scientific). Labeling was performed according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The samples and one reference sample were labeled with 10-plex tandem mass spectrometry tags (TMT) using the standard TMT labeling protocol (Thermo Fisher). To control labeling efficiency and overall instrument performance ...
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bioRxiv - Cell Biology 2024Quote: ... Peptides were collected by centrifugation and labelled using tandem mass tag (TMT) 11-plex isobaric mass tagging reagents (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... 25 μg of peptides per condition were labelled with isobaric tags by incubation for 1 hour at RT (TMT 6-plex and 10-plex, Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2023Quote: ... and the HA-tag was stained with 2.5 µg/mL (1:400) rabbit anti-HA (RM305) (Thermo Fisher, Cat.#MA5-27915) and 10 µg/mL (1:200 ...
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bioRxiv - Cell Biology 2023Quote: ... The peptides were redissolved in 100mM TEAB buffer and equal amounts of peptides from each sample were labelled with TMT 10-plex Mass Tag labelling kit (#90110, ThermoFisher Scientific). After labelling ...
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bioRxiv - Biochemistry 2023Quote: Human GRK2 S670A with a C-terminal hexahistidine tag was expressed in Spodoptera frugiperda (Sf9) cells using the Bac-to-Bac insect cell expression system (Life Technologies). The insect cells were harvested 48 hours post-infection and homogenized with buffer containing 20 mM HEPES pH 8.0 ...
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bioRxiv - Microbiology 2023Quote: ... All collected fractions (3 ml) were analyzed using SDS-PAGE and Western blotting with an anti-His-tag antibody (ThermoFisher Scientific).
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bioRxiv - Neuroscience 2023Quote: ... Peptides were labeled for two hours at room temperature with 0.2 mg of Tandem Mass Tag (TMT) reagent (TMT™ Isobaric Label Reagent Set, Thermo Fisher Scientific ...
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bioRxiv - Genetics 2023Quote: ... We used Gibson Assembly to add a 3x FLAG tag to the appropriate end and insert the tagged coding sequence into a pcDNA5/FRT vector (Thermo Fisher). We note that we overexpressed the propeptide of PSMB4 (removing amino acids 2-45).
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bioRxiv - Biochemistry 2023Quote: Phosphopeptides from different timepoint fractions (three biological replicates) were separately labeled with 16-plex isobaric tandem mass tags (TMTproTM 16plex Label Reagent Set, A44521, Thermo Fisher) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... of two mice age groups (4M and 19M) was taken in a 1.5 mL centrifuge tube for labeling experiment (TMT10plex Isobaric Mass Tag Labeling Kit, Thermo Scientific, #90113). The protein samples’ volume was adjusted with TEAB buffer (100 mM ...
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bioRxiv - Biochemistry 2023Quote: ... MBP-eIF2A-His6 was PCR amplified to incorporate a C-terminal FLAG tag and then subcloned into pFastBac1(Thermo Fisher # 10359016).
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bioRxiv - Cell Biology 2023Quote: ... Peptides in each sample were labeled with one of the tags of TMTpro 18-plex labeling kit (A52045, Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... 6 non-overlapping probes of between 1.2 and 1.7kb covering an area of approximately 12kb were generated using the FISH Tag DNA kit with Alexa Fluor 555 or Alexa Fluor 647 dyes (Invitrogen Life Technologies). 100ng of each probe were added to the 30µL of FHB for hybridization.
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bioRxiv - Biophysics 2023Quote: ... The His14-SUMO tag was separated from cys-ubiquitin by passing the dialyzed eluate over HisPur Ni-NTA Resin (Thermo Scientific). For labeling ...
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bioRxiv - Cell Biology 2023Quote: Copper-inducible pGL1 fly expression vectors for N-terminal FLAG- and HA-tagged cDNAs were generated by using EcoRI and XhoI restriction sites to insert the tag and SalI/NotI restriction sites from pRK5-FLAG or pRK5-HA vectors into a pMT-V5-His backbone (Life Technologies), followed by mutation of 2070C>A to remove a SalI site in the backbone ...
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bioRxiv - Biochemistry 2023Quote: ... human GRK2 S670A with a C-terminal 6xHIS tag was expressed using the Bac-to-Bac insect cell expression system (Life Technologies). The insect cells were harvested 48 hours post-infection and homogenized with buffer containing 20 mM HEPES (pH 8.0) ...