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Citations for Thermo Fisher :
6451 - 6500 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: Tandem mass tag (TMT) labeling was performed for mass spectrometry (MS) using the TMT 6-plex isobaric labeling kit (ThermoFisher, Cat #90111) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... The RBD-6xHIS protein was purified by affinity chromatography of His Tag residues for metals immobilized on the AktaPure system with HisPur ™ Ni-NTA Chromatography Cartridges (Thermo Fisher) column according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Genes were cloned into modified pRSF1-Duet expression vector with a TEV-cleavable N-terminal 14-histidine tag (39) using New England Biolabs and Anza (Thermo Fisher Scientific) enzymes ...
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bioRxiv - Immunology 2020Quote: ... The C-tagged SARS-CoV2 S trimers were purified using a two-step purification protocol by 5 mL CaptureSelect™ C-tag Affinity Matrix (ThermoFisher Scientific). Proteins were further purified by size-exclusion chromatography using a HiLoad Superdex 200 16/600column (GE Healthcare).
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bioRxiv - Molecular Biology 2020Quote: ... at 48 h post transfection and immunoprecipitated using HA tag antibody as per the instructions given in the Pierce Co-Immunoprecipitation kit (Thermo Scientific, USA).
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bioRxiv - Physiology 2020Quote: ... Membranes were blocked with casein before primary antibodies were added (1:3000 dilution of mouse monoclonal antibody against the V5 tag (ThermoFisher MA5-15253)) in a casein buffer containing Tween ...
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bioRxiv - Biophysics 2022Quote: ... The ScPif1-N-His protein was labeled by binding to a 6x-His Tag Monoclonal Antibody (500 nM) conjugated to a DyLight™ 550 Fluorophore (Cat. # MA1-21315-D550, ThermoFisher Scientific).
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bioRxiv - Immunology 2022Quote: ... and cloned Pst I/Not I in a pPPI4 expression vector containing a hexahistidine (his) tag with Gibson Assembly (Thermo Fisher Scientific). All S constructs were verified by Sanger sequencing and the protein was subsequently produced in human embryonic kidney (HEK ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: We removed the GS linker adjacent to the HiBiT tag from HiBiT-FZD7 [13] using GeneArt site-directed mutagenesis (Thermo Fisher Scientific) and the following primers ...
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bioRxiv - Physiology 2022Quote: ... Sample aliquots containing 25 μg of peptides were dried and labeled with tandem mass isobaric tags (TMT 10-plex, Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... Aliquots containing 10 μg of peptide from each sample were labeled with tandem mass tag (TMT) 16-plex reagents (TMTpro, Thermo Fisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: Total HEK293 cell extracts were prepared according to the manufacture’s protocol (Pierce HA-Tag Magnetic IP/Co-IP kit and Pierce Protein A/G Magnetic Beads, Thermo Fisher Scientific, MA). Twenty- four hours after transfection ...
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bioRxiv - Cancer Biology 2024Quote: ... These entry vectors were then subsequently combined with entry vectors containing the pol2 promoter and Flag/Myc-tag into a custom lentiviral destination vector pDest-659 (a gift from Dominic Esposito, NCI) using Gateway LR Clonase (Thermo Fisher Scientific) to generate the final Nat10 overexpression vector ...
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bioRxiv - Cell Biology 2024Quote: ... Expression of the recombinant LZTR1 proteins was confirmed by Western blot analysis using an anti-His tag monoclonal rabbit antibody (Thermo Fisher Scientific). Following confirmation of expression ...
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bioRxiv - Cell Biology 2024Quote: ... The precipitated proteins were visualized by SDS-PAGE and Western blot analysis using an anti-His tag monoclonal rabbit antibody (Thermo Fisher Scientific) to determine the protein distribution in each fraction.
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bioRxiv - Biochemistry 2024Quote: ... HER2 with a C-terminal tail truncation (λ−1030–1255) followed by maltose binding protein (MBP) and twin-strep tags was cloned into pFastBac1 with a CMV promoter (Thermo Fisher Scientific). Point mutations were introduced in the HER2 kinase domain ...
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bioRxiv - Biochemistry 2024Quote: ... hamster (Syrian Hamster Isoform X1 – UniProt: A0A1U8BWQ2) and macaque (Macaque – UniProt: F6SVR2) with an N-terminal cMYC-tag were ordered to GeneArt (Thermo Fisher Scientific) and cloned into a phCMV backbone (GeneBank ...
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bioRxiv - Biochemistry 2024Quote: ... and cloned into a pRK5-derived plasmid with a C- or N-terminal FLAG tag using the gateway vector conversion system (Invitrogen, cat # 11828029). All gene constructs were verified by DNA sequencing.
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bioRxiv - Microbiology 2023Quote: ... was cloned into pFastBac with a C-terminal His6-tag for expression in Sf9 insect cells using the Bac-to-Bac™ Baculovirus Expression System (Thermo Fisher). Following transformation of DH10Bac cells ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... ORP9 and ORP11 cDNAs were custom synthesized by Twist Biosciences to include a C-terminal V5 tag and attB sites and cloned into pDONR221 (Thermo Fisher, 12536017). ORP1 cDNA was purchased in a Gateway compatible pGenDONR vector from GenScript ...
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bioRxiv - Plant Biology 2023Quote: ... Supernatants were mixed with 20 μL anti-HA-agarose beads and incubated overnight following the manufacturer’s instructions (Pierce™ HA-Tag IP/Co-IP Kit, Thermo Fisher Scientific). The HA-tagged protein was eluted and quantified by the Bradford method ...
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bioRxiv - Cell Biology 2023Quote: ... Global proteomic and phosphoproteomic characterization of the samples was achieved by the tandem mass tag (TMT)-based quantitation using the TMT 10-plex reagents (Thermo Fisher Scientific), as previously described (32) ...
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bioRxiv - Biochemistry 2023Quote: Insect cell derived recombinant tag-less eIF2A was expressed in ExpiSf9 cells using the ExpiSf Expression System Starter Kit (Thermo Fisher # A38841) following the manufacturer’s protocol and adapting the IMPACT (Intein Mediated Purification with an Affinity Chitin-binding Tag ...
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bioRxiv - Biochemistry 2023Quote: ... Full-length genes of human RNGTT and CMTR1 were cloned into pMlink vector containing a 4×ProteinA tag at the N-terminal and were transfected to Expi293F cells (Thermo Fisher Scientific) when cell density is 2.5× 106 /ml ...
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bioRxiv - Genetics 2023Quote: ... 6 non-overlapping probes of between 1.2 and 1.7kb covering an area of approximately 12kb were generated using the FISH Tag DNA kit with Alexa Fluor 555 or Alexa Fluor 647 dyes (Invitrogen Life Technologies). 100ng of each probe were added to the 30µL of FHB for hybridization.
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bioRxiv - Cell Biology 2023Quote: ... magnetic beads bound to antibody recognizing the FLAG epitope tag were prepared in-house by coupling Dynabeads M-70 Epoxy (Thermo Fisher Scientific) to FLAG M2 antibody (Millipore Sigma) ...
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bioRxiv - Microbiology 2023Quote: The following primary antibodies were used for confocal microscopy: mouse monoclonal anti- V5-tag (#R960-25, Thermo Fisher Scientific, dilution 1:500), mouse monoclonal anti-α-tubulin (#MA1-19162 ...
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bioRxiv - Biochemistry 2023Quote: ... of randomized RNA library produced for IVM-seq experiment was incubated with equal molarity of 6x His-tag fused target protein complex immobilized on 7 μL Ni-NTA magnetic agarose beads (Thermo Scientific 78605) in the SELEX binding buffer (10 mM Tris pH 8.0 ...
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bioRxiv - Genomics 2023Quote: Levels of H3K27ac CUT&Tag binding signal was determined by qPCR amplification carried out with the QuantStudio™ 5 Real-Time PCR System (ThermoFisher A34322) using the Standard Curve experiment type and SYBR Green Master Mix (ThermoFisher 4309155) ...
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bioRxiv - Cancer Biology 2023Quote: Wild-type and mutant (C139A) USP39 with a Flag-tag at its N-terminal were cloned into the pLenti6/V5 lentiviral vector (Invitrogen, California, USA). Specific shRNA oligonucleotides targeting USP39 were cloned into the pLL3.7 lentiviral vector (Addgene) ...
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bioRxiv - Biochemistry 2023Quote: ... University of Freiburg and cloned into pCDNA5-FRT/To vector with a C-term 3xFLAG-HBH tag (kind gift from Ibrahim Avsar Ilik, Max Plank Institute, Freiburg) using Gateway LR clonase II enzyme kit (Life Technologies 11791020). pSupS1 and pJA2 M1 RNA were kindly provided by Prof ...
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bioRxiv - Genomics 2023Quote: ... Integrity of the GST tag was subsequently checked via western blotting of the purified recombinant protein using anti-GST antibodies (Invitrogen 71-7500) [1:1,500 dilution] ...
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bioRxiv - Neuroscience 2023Quote: ... and then reconstituted directly in 50% acetonitrile in 100mM HEPES (pH 8) containing one of the TMT tags from the TMTpro 18-plex reagent (Thermo Fisher Scientific). The peptide-TMT mixture was incubated in a thermomixer for 1 h at 25°C and 600 rpm ...
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bioRxiv - Biophysics 2023Quote: Cells expressing an endogenous fluorescent tag on alpha-actinin were imaged using the integrated fluorescent light microscope (iFLM) (ThermoFisher Aquilos2 cryoFIB-SEM) to identify cells with ordered sarcomeric arrangement ...
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bioRxiv - Cell Biology 2023Quote: ... -H [fused with a Twin-Strep tag at its C-terminus]) were codon-optimized for Trichoplusia ni and synthesized (GeneArt, Thermo Fisher Scientific). They were inserted stepwise into intermediate plasmid vectors (namely ...
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bioRxiv - Cell Biology 2023Quote: ... labelled for an hour at room temperature using a TMTpro 16plex Isobaric Label Reagent Set (0.8 mg per tag, A44520, ThermoFisher Scientific; LOT VE299607) and following manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... mammalian expression vector with an amino-terminal protein C tag followed with a 3C protease cleavage site and transfected into Expi293 cells (Thermo Fisher Scientific) using FectoPRO (Polyplus-transfection ...
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bioRxiv - Molecular Biology 2024Quote: ... and inserted with an N-terminal FLAG-tag via NheI and NotI restriction sites into the tetracycline-inducible pcDNA5/FRT/TO vector (Thermo Fisher Scientific). For stable integration ...
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bioRxiv - Immunology 2024Quote: ... Each pooled peptide sample was dried in a SpeedVac for 3 hours and then reconstituted directly in a solution consisting of 50% acetonitrile in 100mM HEPES (pH 8) containing the TMT tags from the TMTpro 18-plex reagent (Thermo Fisher Scientific). The peptide-TMT mixtures were incubated in a thermomixer for 1 hour at 25°C and 600 rpm ...
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bioRxiv - Microbiology 2024Quote: ... The constructed vector was subjected to inverse PCR to introduce the 3xFLAG tag to the C-terminus of sfGFP followed by DpnI digest (Thermo Fisher Scientific), 5′ phosphorylation (T4 PNK ...
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bioRxiv - Neuroscience 2022Quote: Primary spinal microglia grown on poly D-lysine coated glass-bottom dishes were loaded with Fura-2 by incubating cells in 2 uM Fura-2-AM (Invitrogen) in growth medium for 30 min at 37℃ ...
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bioRxiv - Immunology 2023Quote: ... 1-2 x 106 antigen-experienced 5c.c7 TCR-transgenic T-cells were incubated with 2 µM Fura-2-AM (Life technologies) in T-cell growth medium for 15-20 minutes at room temperature and subsequently washed with warm (room temperature ...
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bioRxiv - Genomics 2023Quote: ... 2 µL of Superscript IV enzyme, 2 µL of 100 mM DTT and 2 µL of RNAseOut rnase inhibitor, Invitrogen) were added to each well ...
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bioRxiv - Bioengineering 2022Quote: ... formalin-fixed fibrotic capsule tissue samples (n=2 per group) and subcutaneous tissue samples (n=2) were placed in a solution of 2 mg/ml FITC (Invitrogen) in DMEM/F12 media (Gibco) ...
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bioRxiv - Biophysics 2020Quote: ... cells were washed twice with ice-cold phosphate buffered saline (PBS) and incubated in PBS with 1mg/mL EZ-Link Sulfo-NHS-SS-Biotin (ThermoFisher Scientific, PI21331) for 30 min ...
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bioRxiv - Cell Biology 2020Quote: ... supernatant was discarded and fill-in mix was added (38 μM Biotin-14-dATP [Thermo Fisher Scientific], 38 μM dCTP, dGTP and dCTP [Thermo Fisher Scientific] ...
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bioRxiv - Cell Biology 2020Quote: ... nuclei were spun down and resuspended in fill-in mix (biotin-14-dATP [Thermo Fisher Scientific], dCTP, dGTP and dTTP [Thermo Fisher Scientific] ...
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bioRxiv - Biophysics 2019Quote: ... Purified Cas1-Cas2 complex was buffer-changed with 0.5 M Sodium acetate (pH 5.5) and labelled with EZ-Link™ Biotin-LC-Hydrazide (Thermo Scientific, Cat# 21340) and incubated overnight at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... and incubated for 25 mins at ambient temperature in 0.5 mg/ml of EZ-Link™ Sulfo-NHS-LC-Biotin (Thermo Fisher Scientific). To quench biotinylation ...
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bioRxiv - Microbiology 2021Quote: ... samples were UV cross-linked and the biotin signal detected with HRP–conjugated streptavidin using the Chemiluminescent Nucleic Acid Detection Module (Thermo Fisher Scientific) according to the manufacturer’s instructions.