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Citations for Thermo Fisher :
6151 - 6200 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: Copper-inducible pGL1 fly expression vectors for N-terminal FLAG- and HA-tagged cDNAs were generated by using EcoRI and XhoI restriction sites to insert the tag and SalI/NotI restriction sites from pRK5-FLAG or pRK5-HA vectors into a pMT-V5-His backbone (Life Technologies), followed by mutation of 2070C>A to remove a SalI site in the backbone ...
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bioRxiv - Biophysics 2023Quote: ... The His14-SUMO tag was separated from cys-ubiquitin by passing the dialyzed eluate over HisPur Ni-NTA Resin (Thermo Scientific). For labeling ...
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bioRxiv - Biochemistry 2023Quote: ... human GRK2 S670A with a C-terminal 6xHIS tag was expressed using the Bac-to-Bac insect cell expression system (Life Technologies). The insect cells were harvested 48 hours post-infection and homogenized with buffer containing 20 mM HEPES (pH 8.0) ...
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bioRxiv - Neuroscience 2023Quote: ... the virally expressed GFP or YFP was enhanced using the rabbit anti-GFP Tag Polyclonal Antibody-Alexa Fluor 488 (Invitrogen #A21311). In the viral rescue experiment (Figure 5A-B) ...
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bioRxiv - Cell Biology 2023Quote: ... dried peptides were resuspended in 50 μL of 100 mM TEAB pH 8.5 and labeled using two tags from the TMT10plex Isobaric Labeling Kit (Thermo Fisher Scientific) per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Cells were incubated overnight at 4°C with a primary antibody directed against the V5-tag (ThermoFisher, 46-0705, 1:200) or against small double-stranded RNAs (J2 antibody ...
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bioRxiv - Genetics 2023Quote: ... The presence of recombinant CENH3 proteins on the membrane was always verified by detection with the HisG epitope tag antibody (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... followed by first strand cDNA synthesis using either a NSP4 gene-specific forward primer with a random nucleotide tag sequence (5’-cggtcatggtggcgaataaGCGGCCTTTAATGTGGAATG-3) or without any primer according to the SuperScriptTM III Reverse Transcriptase protocol (Invitrogen, 18080044). cDNA synthesis was then completed followed by a PCR using an eGFP gene-specific reverse primer (5’-CACCTTGATGCCGTTCTTCT-3’ ...
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bioRxiv - Cell Biology 2023Quote: ... was added with His-tag and inserted into pcDNA3.4 TOPO vector by pcDNA3.4 TOPO TA cloning kit (Thermo Fisher Scientific, MA) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... and the His-tag was cut off overnight at 4 °C by the addition of 10 U AcTEV Protease (Thermo Fisher). ErCry4a was further purified using anion-exchange chromatography as previously described44 ...
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bioRxiv - Biochemistry 2023Quote: ... The solubilized denatured CPY* was depleted of His14 tag or full-length His14 containing species by three successive passes over 2mL of HisPur Ni-NTA Resin (Thermo Scientific). The urea was dialyzed out over 20 hours bringing the final urea concentration to ∼1mM in buffer Y1 ...
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bioRxiv - Systems Biology 2023Quote: PISA was carried according to the published protocol8 optimized for higher proteome depth and 18-plex multiplicity using Tandem Mass Tag (TMT) pro (Thermo Fisher). Sample processing for proteomics based on nanoscale liquid chromatography and tandem mass spectrometry (nLC-MS/MS ...
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bioRxiv - Cell Biology 2023Quote: From Expi293 cells: Constructs in pCMV10 vector with N terminal 3xFLAG tag were transfected into Expi293F cells (A14527; Thermo Fisher Scientific) with PEI (Polyscience ...
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bioRxiv - Genetics 2023Quote: ... We used Gibson Assembly to add a 3x FLAG tag to the appropriate end and insert the tagged coding sequence into a pcDNA5/FRT vector (Thermo Fisher). We note that we overexpressed the propeptide of PSMB4 (removing amino acids 2-45).
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bioRxiv - Cell Biology 2023Quote: ... All the IHO1 truncations were expressed with the 3C HRV cleavable N-terminal MBP tag in suspension culture of High Five™ Cells (Invitrogen) in Sf-900™ III SFM medium ...
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bioRxiv - Biochemistry 2023Quote: Phosphopeptides from different timepoint fractions (three biological replicates) were separately labeled with 16-plex isobaric tandem mass tags (TMTproTM 16plex Label Reagent Set, A44521, Thermo Fisher) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: Peptides were resuspended in 200 mM HEPES (pH 8.5) and a 100µg aliquot of peptides was mixed with tandem mass tags (TMT, ThermoFisher Pierce, Rockford, IL) at a label to protein ratio of 2:1 ...
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bioRxiv - Synthetic Biology 2023Quote: ... and the HA-tag was stained with 2.5 µg/mL (1:400) rabbit anti-HA (RM305) (Thermo Fisher, Cat.#MA5-27915) and 10 µg/mL (1:200 ...
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bioRxiv - Cell Biology 2023Quote: ... The peptides were redissolved in 100mM TEAB buffer and equal amounts of peptides from each sample were labelled with TMT 10-plex Mass Tag labelling kit (#90110, ThermoFisher Scientific). After labelling ...
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bioRxiv - Biochemistry 2023Quote: ... coli Methionine Sulfoxide Reductase A and B containing N-terminal His tags downstream of a T7 promoter were synthesized (Invitrogen GeneArt) and transformed into BL21(DE3 ...
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bioRxiv - Bioengineering 2023Quote: ... from each sample was then used for isobaric tandem mass tag (TMT) labeling as per the manufacturer’s instructions (Thermo Fisher Scientific). After two hours of incubation at room temperature ...
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bioRxiv - Microbiology 2023Quote: Changes in protein secretion were relative quantified with the isobaric standard tandem tag (TMT) 10 plex labelling kit (Thermo Fisher Scientific).
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bioRxiv - Systems Biology 2023Quote: We conducted mass spectrometry analysis of 120 CSF samples using 13 batches of 11-plex tandem mass tag (TMT, Thermo Scientific) experiments ...
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bioRxiv - Cell Biology 2024Quote: ... These two constructs were modified in FL and dC by a fragment comprising a 6xHis tag and a SpeI recognizing site synthesized by GeneArt (Life Technologies) and inserted between the SgrAI and MfeI sites present at the C-terminal end of mCherry (restriction enzymes provided by NEB) ...
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bioRxiv - Neuroscience 2024Quote: ... Equal amounts of digested protein (60 µg) were removed from each sample and labeled using TMTpro 16plex Mass Tag Labelling Kit (TMT; cat. # A44520, Thermo Fisher) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Peptides were labeled with isobaric tandem mass tag (TMT) reagents according to the manufacturer’s instructions (TMT11TM Isobaric Label Reagent Set, Thermo Fisher Scientific). Sample multiplexes were reversed-phase fractionated (basic pH ...
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bioRxiv - Microbiology 2024Quote: ... Peptides were then labelled using an isobaric labelling-based approach, relying on tandem mass tags (TMT, (Thompson et al., 2003)) using the 16plex TMTpro isobaric Label Reagent kit (ThermoFisher Scientific) before mixing equivalent amounts and desalting using C18 reverse phase chromatography (Sep-Pak C18 ...
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bioRxiv - Developmental Biology 2024Quote: ... the exoA coding sequence was fused with a C-terminal mCherry tag followed by the N-terminal half of GFP sequence and inserted into the pIB-V5 vector (Thermo Scientific). Plasmid SOD2-cGFP was previously published 43 ...
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bioRxiv - Physiology 2024Quote: ... labelled with Tandem Mass Tag (TMT) ten plex reagents according to the manufacturer’s protocol (Thermo Fisher Scientific, Loughborough, LE11 5RG, UK). Labelled samples were pooled and 50μg was desalted using a SepPak cartridge (Waters ...
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bioRxiv - Biophysics 2020Quote: Oocytes expressing Wild-type Cx30 or Cx30G11R were washed out with cold PBS and then incubated with PBS containing 0.5 mg/mL EZ-LinkTM NHS-Biotin (Thermo Fisher Scientific) for 10 minutes at 4°C ...
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bioRxiv - Immunology 2021Quote: ... and wild type or IL-7rα-/- (both CD45.2+) were depleted of lineage-positive cells by magnetic separation using Biotin Binder Dynabeads (Thermo Fisher Scientific) after staining with biotinylated antibodies against CD3 ...
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bioRxiv - Genetics 2021Quote: ... and the amount of Nb127D01-biotin bound to the plate was quantified through the addition of streptavidin-HRP (Thermo Fisher, N100), washing ...
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bioRxiv - Genomics 2020Quote: ... The DNA overhangs were filled with biotinylated nucleotides by addition of 100 μL pre-mix (25 μL 0.4 mM Biotin-dATP (Thermo Fisher, 19524016), 25 μL 0.4 mM Biotin-dCTP (Thermo Fisher ...
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bioRxiv - Molecular Biology 2021Quote: ... biotin labelled Hi-C 3.0 DNAs were purified by 20 μl Dynabeads® MyOne™ Streptavidin C1 beads (Thermo Fisher 65002). The biotinylated-DNA on C1 beads was used to perform on-beads library making with NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... 400 ng of total RNA was labeled with biotin and hybridized to the miRNA 4.0 Array (ThermoFisher Scientific, Waltham, MA, #902412) for 18 hours at 48°C using an Affymetrix® 450 Hybridization Oven (Affymetrix ...
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bioRxiv - Molecular Biology 2021Quote: ... The 4sU-labelled RNA was thiol-specific biotinylated by addition of 200 μg EZ-link HPDP-biotin (ThermoFisher Scientific, Cat# 21341), biotinylation buffer (10 mM Hepes-KOH pH 7.5 and 1 mM EDTA ...
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bioRxiv - Molecular Biology 2020Quote: ... Restriction fragments were biotinylated by supplementing the reaction with 60 μl of fill-in mix (0.25 mM biotin-14-dATP (Life Technologies, 19524016), 0.25 mM dCTP ...
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bioRxiv - Immunology 2022Quote: ... if required by the assay and excessive biotin was removed by size exclusion chromatography using Zeba-Spin desalting columns (7kDa cutoff, Thermo Fisher).
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bioRxiv - Genomics 2020Quote: ... Both the template strand and its reverse complement were labeled using the Pierce Biotin 3’ End Labeling Kit (Thermo Fisher Scientific) and the complementary oligonucleotides were annealed ...
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bioRxiv - Developmental Biology 2019Quote: ... signal amplification was performed using biotin anti-rabbit (JacksonImmunoResearch) coupled antibody at 1:500 and streptavidin-Cy3 (or-488) conjugate at 1:500 (Molecular Probes). Immunofluorescent stainings on mouse intestinal organoids were realized as previously described (O’Rourke K. ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were harvested before the cultures reached OD600 = 1 and were labeled with 8 mg/ml EZ-Link Sulfo-NHS-LC-biotin (ThermoFisher Scientific) for 30 minutes at 4°C ...
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bioRxiv - Cancer Biology 2019Quote: ... the protein sample was reacted with biotin-alkyne using the Click-iT Protein Reaction Buffer Kit (Cat. no. C10276, Thermo Scientific) following protocols in the instruction sheet ...
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bioRxiv - Biochemistry 2019Quote: ... the antibody-coupled beads were washed three times in 100 μl PBS-T before labelling the antibodies with a 150x molar excess of EZ-Link-NHS-PEG4-Biotin (21329, Thermo Scientific) dissolved in DMSO (276855 ...
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bioRxiv - Microbiology 2020Quote: ... Wells were washed three times as above then incubated at room temperature with 50μl/well biotin-labelled rabbit monoclonal anti-His6 (Thermo Fisher Scientific) diluted to 1/1000 in AB for 1 hr ...
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bioRxiv - Immunology 2019Quote: AF647 and biotin-conjugated anti-mouse FCRL6 (1C3 and 3C1) were generated and labeled by our laboratory (Invitrogen and Thermo Scientific). Anti-VH11 (3H7 (Rowley et al. ...
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bioRxiv - Immunology 2019Quote: AF647 and biotin-conjugated anti-mouse FCRL6 (1C3 and 3C1) were generated and labeled by our laboratory (Invitrogen and Thermo Scientific). Anti-VH11 (3H7 (Rowley et al. ...
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bioRxiv - Biochemistry 2019Quote: CMG2-GST (AlexaFluor 488 or biotin) and PA-E733C (AlexaFluor 546) were conjugated on a single reactive cysteine with maleimide-labels (Thermo Fisher) after GST was cleaved by using thrombin ...
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bioRxiv - Cancer Biology 2020Quote: ... The purified RNA obtained from IVT was labelled with biotin at the 3’end using the PierceTM RNA 3′End Desthiobiotinylation Kit (ThermoFisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2021Quote: ... and biotin-PEG-SVA at a ratio of 99:1 (w/w) (Laysan Bio) in 0.1 M sodium bicarbonate (Thermo Fisher Scientific) for 3 h.10 Excess PEG was rinsed with water ...
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bioRxiv - Bioengineering 2020Quote: A cell line derived from Expi293 cells expressing an ER-localized biotin ligase (BirA) gene was grown in Expi293 media (Thermofisher Scientific) supplemented with 100 µM biotin (GoldBio ...