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Citations for Thermo Fisher :
6201 - 6250 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... α-Syn overexpressing neuronal cultures and non-transduced neuronal cultures were washed three times with cold PBS and incubated with sulfo-NHS-biotin (1.5 mg/ml; ThermoFisher Pierce, 21331) for 30 minutes at 4 °C with rocking ...
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bioRxiv - Microbiology 2020Quote: ... Unreacted nanobody and sortase was removed with Ni-NTA resin and excess GGGK-biotin was removed using Zeba spin desalting columns (0.5 mL, 7k MWCO, Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2021Quote: ... N2a cells were washed twice with cold PBS and incubated for 30 min with 0.5 mg/mL EZ-Link Sulfo-NHS-SS-Biotin (Thermo Fisher Scientific) in PBS shaking at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... The neurons were incubated with 1.5 mg/mL EZ-Link™ Sulfo-NHS-S-S-Biotin (#21331; Thermo Fisher Scientific, USA) in PBS for 30 minutes at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Biotin-labelled samples were then immunoblotted and visualized with Streptavidin-HRP antibody on an iBright™ FL-1500 imaging system (ThermoFisher).
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bioRxiv - Biochemistry 2022Quote: ... The labelled lysate was then clicked on with biotin-alkyne using copper catalyzed azide-alkyne click chemistry reaction protocol according to manufacturer’s instruction (ThermoFisher Scientific C33372). The biotinylation was detected using IRDye 800CW Streptavidin dye and imaged (Odyssey Image System LICOR).
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bioRxiv - Neuroscience 2021Quote: ... Two slices were incubated in in chilled ACSF containing 1 mg/ml EZ-link Sulfo-NHS-SS-Biotin (Thermo Fisher Scientific) for 45 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then washed twice with ice cold PBS (4° C) and subsequently transferred to cold PBS containing Ez-link Sulfo-NHS-LC-biotin (1 mg/ml; ThermoFisher Scientific) for 45 min ...
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bioRxiv - Neuroscience 2021Quote: ... acute hippocampal slices were labeled for 30 min at 4 °C with 1 mg/ml sulfo-NHS-SS biotin (ThermoFisher Scientific). Membranes were prepared and the biotinylated proteins were precipitated with streptavidin agarose resin (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... The collected supernatant was then subjected to dialysis to remove excess free biotin using a Slide-A-Lyzer MINI dialysis device (Thermo Scientific). Proteins were precipitated by adding 9 parts methanol to 1 part lysate ...
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bioRxiv - Microbiology 2021Quote: ... Typhimurium was grown in BHI broth until OD600:1 and then biotinylated with EZ-link NHS-Biotin reagent (Thermo Fisher Scientific). After washing ...
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bioRxiv - Microbiology 2021Quote: ... cells were washed once with PBS (pH 8) and incubated with 1 mg/ml of EZ-link Sulfo-NHS-biotin (Thermo Fisher) in PBS (pH 8 ...
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bioRxiv - Cell Biology 2022Quote: ... Five hundred ng of high-quality RNA was hybridized to biotin and subsequently onto Affymetrix Mouse Genome 430 2.0 GeneChips according to the manufacturer’s protocols (900497, Applied Biosystems, Waltham, MA). Thereafter ...
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bioRxiv - Bioengineering 2019Quote: ... the GVs were biotinylated by incubating them for 1 hour with a 10,000-fold molar excess of sulfo-NHS-biotin (Thermo Fisher Scientific). Then ...
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bioRxiv - Molecular Biology 2019Quote: ... the slides were washed in 2X SSC and biotin was detected with Streptavidin AF 488 (ThermoFisher S-32354; Lot# 1571714, 1977355). Slides were mounted and counterstained in Vector shield mounting medium with DAPI (Vector laboratories ...
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Gatekeeper helix activates Golgi SM protein Sly1 and directly mediates close-range vesicle tetheringbioRxiv - Cell Biology 2020Quote: ... content mixing FRET reporters were then added (500 µL of 4 mg/mL solution of R-phycoerythrin-biotin conjugate, or 296 µL of 2mg/mL Alexa-Streptavidin; both reagents from Thermo/Molecular Probes). SNARE stocks in SNARE elution buffer with β-OG were then added to a final molar ratio of 1:600 (each Q-SNARE ...
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bioRxiv - Immunology 2020Quote: ... Cells were stained with human MR1-5-OP-RU tetramer for 40 minutes at RT in the dark prior to the addition of anti-bovine CD8-biotin (Clone ILA51) and Near-Infrared Fixable LIVE/DEAD stain (Invitrogen or Molecular Probes) for 20 minutes at 4 °C ...
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bioRxiv - Cancer Biology 2020Quote: M28 and BPH-1 cells were surface biotin-labeled with EZ-Link Sulfo-NHS-LC-Biotinylation Kit (Pierce/Thermo Fisher Scientific) and lysed with cell lysis buffer (20 mM Tris-HCl ...
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bioRxiv - Plant Biology 2020Quote: ... upstream of the start codon were labeled using the Biotin 3’ End DNA Labeling Kit according to the manufacturer’s instructions (Thermo Scientific/Pierce). EMSA was performed with the Light Shift Chemiluminescent EMSA Kit (Thermo Scientific/Pierce) ...
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bioRxiv - Cell Biology 2019Quote: ... The cell surface was biotinylated by incubating cells for 10 min with 1 mg of EZ-Link-Sulfo-NHS-LC-biotin (Thermo Scientific) in Sorensen-Sorbitol buffer at pH 8 on ice ...
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bioRxiv - Bioengineering 2019Quote: GV clusters were prepared by first biotinylating purified GVs with 105 molar excess of EZ-Link Sulfo-NHS-LC-biotin (Thermo Scientific) in PBS for 4 hours ...
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bioRxiv - Immunology 2019Quote: ... Viability was determined using LIVE/DEAD Fixable Aqua Dead Cell Stain kit (DCM) for 405 nM excitation and biotin-conjugated antibodies were visualized using Streptavidin-QD705 (Life Technologies). Cells were fixed and permeabilized using Fixation/Permeabilization Kit (eBioscience ...
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bioRxiv - Cell Biology 2019Quote: ... The cell surface was then biotinylated by incubating cells for 3 min with 30 mg of EZ-Link-Sulfo-NHS-LC-Biotin (Thermo Scientific) in Sorensen-Sorbitol buffer at pH 8 on ice ...
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bioRxiv - Neuroscience 2020Quote: Rodent astrocytes were plated in 6 well plates at a density of 500,000 cells/well and live cells were treated with EZ-LinkTMNHS-Biotin reagent (Thermo Scientific, 20217). Cells were then homogenized and pelleted ...
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bioRxiv - Immunology 2021Quote: ... for human serum or plasma derived antibodies or a 1:25 dilution of biotin goat anti-rat or anti-mouse IgG antibody (A16088, Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2021Quote: ... cells were washed twice with ice-cold PBS2+ (with Ca2+ and Mg2+) and incubated with 0.5 mg/ml EZ-Link Sulfo NHS-Biotin (Thermo Fisher Scientific) for 30 min on ice ...
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bioRxiv - Biochemistry 2021Quote: Protein content in cell culture lysates and eluates from biotin/streptavidin or acyl-RAC mediated protein purification was determined by the BCA assay (Pierce, Thermofisher Scientific) and mixed with 4x reducing Laemmli buffer to yield the indicated protein amounts in 1x Laemmli buffer ...
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bioRxiv - Physiology 2020Quote: ... TsA-201 cells transiently expressing Nav1.5 constructs were supplied with 0.5 mg/mL EZ-link™ Sulfo-NHS-SS-Biotin (Thermo Fisher Scientific) in PBS for 15 min at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... Biotinylation of cell surface proteins using a non-cell permeable and thiol-cleavable probe was initiated by incubating cells with 0.4 mM EZ-Link Sulfo-NHS-SS-Biotin (Thermo Fisher Scientific) in DPBS+ buffer for 30 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... and either PFA-fixed at indicated times for immunofluorescence imaging or subjected to incubation on ice with Sulfo-NHS-SS-Biotin (Thermo Scientific) to biotinylate all surface proteins ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Cell surface proteins were labeled by incubating cells with 1.6 mM Sulfo-NHS-SS-Biotin (Thermo Fisher Scientific, Waltham, MA USA) or 1.6 mM Biotin-dPEG3-Maleimide (MilliporeSigma ...
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bioRxiv - Bioengineering 2021Quote: ... biotin-streptavidin binding was used to functionalize magnetic particles (Dynabeads MyOne™ Streptavidin C1, Cat. #65001, Life Technologies, Carlsbad, CA, USA) with antibodies for CTXII (cat ...
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bioRxiv - Immunology 2020Quote: ... The reduced polysaccharide was passed through two Bio-gel P2 mini columns and the level of biotinylation was detected with Pierce Biotin Quantitation Kit (28055, Thermo Scientific).
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bioRxiv - Microbiology 2022Quote: ... PCR products (1□μg) of desired templates were 3’ end-labeled using a Pierce biotin 3’ End DNA Labeling Kit (Thermo Scientific). The resulting probe reaction mixtures were electrophoresed on a 0.8% agarose gel for 30 min at 100 V and then gel purified with a NucleoSpin Gel and PCR Clean-up kit (Takara Bio USA) ...
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bioRxiv - Developmental Biology 2022Quote: ... Biotin pulldown was performed by adding an equal volume of Hi-C sample with Dynabeads MyOne Streptavidin T1 beads (Invitrogen, 65602) and incubated by 15 minutes with rotation ...
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bioRxiv - Biochemistry 2022Quote: Purified protein at a concentration of 1 mg/ml was chemically biotinylated using EZ link NHS-PEG4-Biotin (Thermo Scientific, A39259) at a 10-30 times molar excess ...
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bioRxiv - Biophysics 2022Quote: 96 well glass plates (Matek, PBK96G-1.5-5-F) were incubated with 80 μL of 100 ug/ml BSA-Biotin (Thermo Fisher, 29130) in PBS for 2 hours at room temperature ...
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bioRxiv - Biophysics 2022Quote: ... Briefly, ligands (DinB, DinBΔPAD, and DinB-PAD) were biotinylated using the biotinylation kit EZ-Link NHS-PEG4-Biotin (Thermo Fisher Scientific). The biotin label was freshly dissolved in H2O ...
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bioRxiv - Microbiology 2022Quote: ... 1 nM biotin-labeled DNA and varying concentrations of either CBUA0028 or CBUA0027-CBUA0028 in 1X Binding Buffer (LightShift Chemiluminescence kit; Thermo Scientific), were assembled and incubated at room temperature for 30 min ...
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bioRxiv - Plant Biology 2022Quote: ... The electrophoretic migration of biotin-labeled probes was resolved on 6% native polyacrylamide gels and detected using an enhanced chemiluminescence substrate (Thermo Scientific).
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bioRxiv - Molecular Biology 2023Quote: ... slides were washed 2× in PBS before EdU-Biotin Azide conjugation by click chemistry using the Click-iT reaction (Click-iT assay kit (Thermo Fisher, according to the manufacturer’s instructions) ...
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bioRxiv - Microbiology 2022Quote: ... 1mM DTT, 300 mM KCl, 20µM biotin-11-ATP, -UTP, -GTP, -CTP, 0.4 U/ml RNase inhibitor [RNaseOUT ThermoFisher] and 1% Sarkosyl) was added to thawed nuclei ...
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bioRxiv - Molecular Biology 2022Quote: ... targeting receptor-binding domains of SARS-CoV-2 spike glycoprotein which were discovered in Song et al.22 Biotin-modified aptamers and ACE2 were bound to streptavidin-coated polystyrene plates (ThermoFisher #PI15500). The aptamers and ACE2 captured the recombinant spike protein ...
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bioRxiv - Biophysics 2022Quote: ... 20–25 oocytes per sample were washed x6 in ND96 and labeled with 0.5 mg/ml EZ-Link™ Sulfo-NHS-SS-Biotin (Thermo Fisher) for 30 min at room temperature ...
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bioRxiv - Pathology 2024Quote: ... with a biotin conjugate was used (1:200) followed by AB reagent or streptavidin conjugated with AlexaFlour 488/594 fluorophore (ThermoFisher Scientific). For IF detection ...
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bioRxiv - Immunology 2024Quote: ... and soluble membrane protein (SMP) preps were extracted from Chinese hamster ovary (CHO) cells and were biotinylated using an NHS-LC-Biotin kit (ThermoFisher Scientific). Yeast displaying IgGs on their surface were incubated with biotinylated SCP and SMP preps at a 1:10 dilution in PBSF (PBS with 0.1% w/v BSA ...
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bioRxiv - Microbiology 2024Quote: ... A 1 mL suspension of the bacterial cells was mixed with EZ-Link Sulfo-NHS-LC-Biotin (Thermo Fisher Scientific, #A39257) dissolved in 100 µl of sterile MilliQ water ...
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bioRxiv - Developmental Biology 2024Quote: Transfected COS7 cells on a 100-mm dish were biotinylated by incubation in 1 mg/ml NHS-SS-Biotin (21331, Thermo Scientific), diluted in 1× PBS containing 1 mM MgCl2 and 0.1 mM CaCl2 (PBS–MC ...
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bioRxiv - Molecular Biology 2024Quote: ... The resulting chains were then incubated overnight at room temperature with 10x molar excess of EZ-Link Maleimid-PEG2-Biotin (Thermo Scientific). The next day ...
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bioRxiv - Neuroscience 2024Quote: ... The cell culture medium was removed and cells were suspended with 1 mL ice-cold PBS and then treated with 1 mL sulfo-N-hydroxysuccinimide-SS-biotin (Thermo Scientific) (1 mg/mL in PBS ...