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Citations for Thermo Fisher :
6501 - 6550 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... formed covalent complexes between cDNA display molecules and pentylamine-biotin were pulled from the mixture by immobilization to streptavidin-coated magnetic microbeads (20 μL; Streptavidin MyOne C1, Thermo Fisher, USA). After immobilization ...
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bioRxiv - Biochemistry 2020Quote: Coverslips were washed with PBS and then click reaction was performed by incubating cells for 30 min with 100 μl of click reaction buffer containing 20 μM biotin azide (ThermoFisher or Jena), 2 mM CuSO4 ...
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bioRxiv - Neuroscience 2021Quote: ... The slices were transferred into bubbled ice-cold aCSF containing 1 mg/ml EZ-Link™ Sulfo-NHS-Biotin (21217, Thermo Scientific) with gentle rotation for 45 min at 4 °C ...
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bioRxiv - Cancer Biology 2021Quote: Cells were grown on 6-well plates until 80-90% confluency and cell surface proteins were biotinylated with EZ-Link NHS-PEG4-Biotin (ThermoFisher Scientific, #21330) at 0.5 mg/ml in HBSS (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... Milli-Q until total volume of 1,000 ml) followed by adding 0.5 mg/ml Sulfo-NHS-LC-LC-biotin (Thermo fisher scientific, Rockford, USA). After 30 minutes incubation ...
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bioRxiv - Synthetic Biology 2021Quote: ... An imaging glass chamber was incubated for 5 min with a mix of bovine serum albumin (BSA) and BSA-biotin (1:1 molar ratio, 1 mg mL−1, Thermo Fisher Scientific), followed by incubation with Neutravidin (1 mg mL−1 ...
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bioRxiv - Systems Biology 2020Quote: ... Constructs containing the gene of interest fused to the biotin ligase and a FLAG epitope were generated via Gateway cloning technology (Thermo Fisher Scientific). Firstly ...
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The pan-cancer lncRNA PLANE regulates an alternative splicing program to promote cancer pathogenesisbioRxiv - Cancer Biology 2020Quote: ... 4 μg antisense / sense biotin-labelled probes were incubated with lysates at 4 °C overnight before rotating with streptavidin beads (ThermoFisher Scientific, #20349) for additional 2 hr ...
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bioRxiv - Cancer Biology 2021Quote: The cell surface of KP4 cells and MYOF-HA expressing HEK293T cells was biotinylated on ice using 0.5 mg/ml of EZ-Link™ Sulfo-NHS-SS-Biotin (Thermo Scientific: 21331) for 30min ...
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bioRxiv - Microbiology 2022Quote: ... The cells were washed with ice-cold PBS for three times and labeled with 1 mg/mL EZ-Link Sulfo-NHS-Biotin (Thermo Fisher Scientific) at 4 °C for 1 h ...
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Pathogenic variants of sphingomyelin synthase SMS2 disrupt lipid landscapes in the secretory pathwaybioRxiv - Cell Biology 2022Quote: ... cells were washed three times with ice-cold PBS and then incubated for 30 min at 4°C in PBS containing 1 mg/ml EZ-Link-sulfo-NHS-LC-LC-Biotin (Thermo Fisher Scientific). Next ...
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bioRxiv - Plant Biology 2021Quote: The probes used for EMSA were 3’-biotin end-labeled by HuaGene (Shanghai, China) and EMSA was conducted using a LightShift Chemiluminescent EMSA kit (Thermo Fisher Scientific). Mutant probes and competition probe (20-fold and 500-fold unlabeled oligonucleotides ...
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bioRxiv - Neuroscience 2021Quote: ... 500 µg of synaptosomes were incubated 60 min at 4 °C with sulfo-NHS-biotin (1.5 mg/ml) in 500 µl of PBS/Ca+/Mg+ pH 7.4 (ThermoFisher Scientific, Waltham, MA, USA). Biotinylation reaction was quenched by incubating the synaptosomes with 1 vol of PBS containing glycine 100 mM for 15 min ...
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bioRxiv - Biophysics 2021Quote: ... digoxigenin- and biotin-labeled sense and anti-sense primers (Integrated DNA Technologies, Coralville, IA, or Invitrogen, Life Technologies, Grand Island, NY, USA) (Table S1) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the tissues were stained with isolectin B4 from Griffonia simplicifolia (GS-IB4; Biotin-conjugated, 1:250 dilution, Invitrogen, Waltham, MA, USA #121414) and anti-PRMT5 (1:150 dilution ...
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bioRxiv - Cell Biology 2022Quote: ... cells that had been incubated for 120 min with 500 µM biotin were fixed and permeabilized as described above and were incubated with Alexa Fluor 647 streptavidin (Thermo Fisher, S21374) diluted 1:750 ...
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bioRxiv - Genomics 2019Quote: ... The Hi-C library was created by shearing 20 ug of DNA and capturing the biotin-containing fragments on streptavidin-coated beads using Dynabeads MyOne Streptavidin T1 (INVITROGEN, Waltham, America). Then DNA fragments were end-repaired and adaptor ligation was performed ...
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bioRxiv - Neuroscience 2019Quote: ... labeled using the Encore Biotin module (Nugen) and loaded for transcriptome analysis on a GeneChip™ Rhesus Macaque Genome Array (ThermoFisher scientific), designed to assess expression levels of transcripts in the macaque monkey genome ...
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bioRxiv - Cancer Biology 2019Quote: ... labeled cDNA targets were generated with the Encore® Biotin Module (Nugen) and hybridized to a GeneChip® Human Transcriptome Array 2.0 (Affymetrix, Thermo Fisher) following manufacturer’s instructions ...
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bioRxiv - Physiology 2020Quote: ... Brain sections stained with guinea pig anti-tdTomato antibody (1:1,000; Frontier Institute) were incubated with biotin-labelled anti-guinea pig IgG (1:250; Invitrogen, Carlsbad, CA, USA) overnight ...
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bioRxiv - Synthetic Biology 2020Quote: ... The sample reservoir of the imaging chamber was functionalized with 1:1 molar ratio of bovine serum albumin (BSA) and BSA-biotin (1 mg mL−1, Thermo Fisher Scientific), and then with Neutravidin (1 mg mL−1 ...
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bioRxiv - Immunology 2020Quote: ... and soluble cytosolic protein (SCP) fractions were extracted from Chinese hamster ovary (CHO) cells and biotinylated using NHS-LC-Biotin (Thermo Fisher Scientific) reagent ...
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bioRxiv - Microbiology 2020Quote: ... and hyphae were washed three times with PBS (pH 7.4) and then incubated in 5 mL of PBS containing 5 mg EZ-Link Sulfo-NHS-LC-Biotin (Thermo Fisher Scientific, 21335) for 1 h at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... Each well was washed with PBS and then incubated for one hour at 4°C while rocking with 1 mg/mL EZ-Link NHS-SS-Biotin (Thermo Fisher Scientific) in PBS ...
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bioRxiv - Genomics 2019Quote: Forward and reverse strand of 21-mer DNA oligos encompassing rs398206 were synthesized with 5’ biotin labeling (Life Technologies; Supplementary Table 15) and were annealed to make double stranded probes ...
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bioRxiv - Immunology 2021Quote: ... S-2P and RBD were biotinylated protein at a theoretical 1:1 ration using the Easylink NHS-biotin kit (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Cells were then stained with biotin-conjugated anti-PE antibody together with antibodies for surface markers for flow cytometric analysis using AttuneNxT (Thermo Fisher Scientific) and FlowJo 10.5.2 (BD Biosciences).
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bioRxiv - Genetics 2019Quote: ... The washed resin was resuspended in 900 ul of elution buffer (1XPBS, 5% SDS, 10 mM D-Biotin (Life technologies, B-20656), transferred to an eppendorf tube and boiled for 15 min ...
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bioRxiv - Neuroscience 2019Quote: ... Following incubation with biotin-conjugated anti-phosphotyrosine (mouse, 1:2000; AbD Serotec, #MCA2472B) and streptavidin-HRP conjugate (1:10000; Thermo Scientific, #21126) the amount of luminescence was determined by incubation with ECL using a plate reader (Varioskan Flash ...
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bioRxiv - Biochemistry 2021Quote: ... Biotinylation reaction was incubated for 30 min at room temperature and the excess biotin was removed with a Zeba™ 7k MWCO desalting column (Thermo Scientific).
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bioRxiv - Molecular Biology 2021Quote: ... followed by separation on 20% 7 M urea SDS-PAGE gel and immunoblot assay with anti-Biotin antibody (Thermo Fisher Scientific, 692033).
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bioRxiv - Cell Biology 2020Quote: ... the cells were washed with washing buffer (PBS containing 1%FBS) and incubated with biotin labelled anti-CD11b antibody (RM2815; Invitrogen; 1:100) for 40min with constant mixing using Hula mixer (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... which was conjugated at a 7:1 molar ratio with the cleavable EZ-Link Sulfo-NHS-SS-Biotin (#A39258, Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Thymocytes from 3- to 4-week-old wild type mice were stained with biotinylated antibodies for lineage markers (excluding CD4) and depleted of lineage-positive cells by magnetic separation using Biotin Binder Dynabeads (Thermo Fisher Scientific). Flt3+ or Flt3-ETP were pre-sorted for yield and then 200 cells/well were sort-purified directly onto the OP9-DLL4 culture plate ...
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bioRxiv - Biophysics 2022Quote: ... Labeled tubulin was prepared by incubating polymerized microtubules with tetramethylrhodamine succinimidyl ester (C-1171; Thermo Fischer) or NHS-LC-biotin (21336; Thermo Fisher Scientific) for 30 min at 37°C (32) ...
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bioRxiv - Immunology 2022Quote: ... and citrullinated Histone 4 using biotinylated peptides (Cit3-His4 bio: SG[Cit]GKGGKGLGKGGAKRHGSGSK-biotin) on streptavidin-coated high-capacity pre-blocked plates (Thermo Fisher Scientific) and hIgG1 at 5 μg/ml in RIA buffer (1% BSA ...
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bioRxiv - Microbiology 2022Quote: ... The biotin-marked ligation products were then enriched by affinity capture with streptavidin magnetic beads (DynaBeads MyOne Streptavidin C1; Invitrogen, Thermo Fisher Scientific). Subsequently ...
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bioRxiv - Microbiology 2022Quote: ... These LY2-specific probes were made by random priming and labeled with biotin using the BioPrime Array CGH Genomic Labeling System (Invitrogen catalog # 18095012). Membranes were incubated with IRDye800 and imaged using Odyssey DLx imaging system (LI-COR).
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bioRxiv - Neuroscience 2023Quote: ... The resulting preformed fibrils (PFFs) were labeled with biotin by using an EZ-Link™ Sulfo-NHS-LC-Biotinylation Kit (Cat#21435, Thermofisher Scientific). After biotinylation ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were washed with ice-cold PBS containing 1 mM CaCl2 and 0.5 mM MgCl2 and incubated with 1 mg/ml Sulfo-NHS-SS- biotin (Thermo Fisher Scientific, PI21331) for 15 minutes on ice ...
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bioRxiv - Neuroscience 2022Quote: ... Amyloid fibrils prepared in the presence of biotin-conjugated cardiolipin were incubated with 1 nM Alexa Fluor 647 (AF647)-streptavidin (Thermo Fisher Scientific) and 5 mM ThT (Sigma Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... holo-Tf was biotinylated for two hours on ice with an EZ-LinkTM Sulfo-NHS-LC-LC-Biotin kit (Thermo Fisher Scientific) and then buffer exchanged into PBS with a 0.5 mL Pierce Protein Concentrator PES column with a 10K MWCO to remove unreacted biotin ...
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bioRxiv - Physiology 2024Quote: OK cells cultured on permeable supports under static or FSS conditions were washed with cold PBS and the apical surface biotinylated with 1 mg/mL EZ-Link Sulfo-NHS-SS-biotin (Thermo Scientific, 21331) in 0.5 mL TEA-buffered saline (TBS ...
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bioRxiv - Molecular Biology 2024Quote: ... The hybridization signal from the biotin-labeled probe (listed in Supplementary Table 1) was detected using the Chemiluminescent Nucleic Acid Detection Module Kit (Thermo Fisher Scientific), following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: RPE1-hTERT cells were washed generously with ice-cold PBS prior to labelling with cell-impermeable 0.2 mg/mL Sulfo-NHS-SS Biotin (ThermoFisher Scientific, no. 21217) in PBS (pH 7.4) ...
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bioRxiv - Microbiology 2024Quote: ... The solution was then mixed with aniline (80 mM; sigma) followed by EZ-link alkoxyamine PEG-4-SS-biotin (40 mM; PEG = polyethylene glycol, Thermo Fisher Scientific). All 4 reagents were mixed in equal volumes ...
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bioRxiv - Cancer Biology 2024Quote: ... Pulldown of biotin-labeled DNA was initiated by separating 10 µL of 10 mg/mL Dynabeads MyOne Streptavidin T1 beads (Life Technologies, 65602) on a magnetic stand ...
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bioRxiv - Cancer Biology 2024Quote: ... 10 μl of sample was saved for 3′ -biotin labeling for visualization using Pierce RNA 3′ End Biotinylation Kit (Life Technologies 20160) according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... to promote DNA synthesis as previously described(Ray-Gallet and Almouzni 2004) except that the reaction mixed contained 3.2 µM of biotin-14-dCTP (Invitrogen, Ref 19518-0189) instead of [α32P]-dCTP ...
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bioRxiv - Plant Biology 2023Quote: ... DNA probes of NHX1 and SAL1 were synthesized and labeled using the Biotin 3′ End DNA Labeling Kit (Thermo Fisher Scientific, 89818). The EMSA was performed using the LightShift Chemiluminescent EMSA kit (Thermo Fisher Scientific ...