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Citations for Thermo Fisher :
6051 - 6100 of 10000+ citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: Plasmid transfections were performed using Lipo2000 transfection reagent (cat no. 11688-030, Invitrogen by ThermoFisher Scientific) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2023Quote: Plasmid transfections were performed using Lipo2000 transfection reagent (cat no. 11688-030, Invitrogen by ThermoFisher Scientific) according to the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2023Quote: ... The digested plasmid and DNA fragment (if necessary) were ligated with T4 DNA ligase (Thermo Scientific) or One Step Cloning Kit (YEASEN ...
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bioRxiv - Molecular Biology 2023Quote: ... empty vector control + PPARγ or PPARγ + ZFP407 plasmids using Lipofectamine 3000 (ThermoFisher Scientific, Waltham, MA, USA) according to manufacturer’s protocol ...
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Measuring carbohydrate recognition profile of lectins on live cells using liquid glycan array (LiGA)bioRxiv - Biochemistry 2023Quote: ... Phage DNA was extracted from the pellet using a GeneJET Plasmid Miniprep kit (Thermo Fisher, #K0502) and analyzed by Sanger sequencing.
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bioRxiv - Biochemistry 2023Quote: ... Plasmid DNA of mtRF1-AAG-3xFLAG in a pcDNA3.1 vector backbone was ordered from Thermo Scientific. DNA and PEI at a ratio of 1:3 (w/w ...
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bioRxiv - Cancer Biology 2023Quote: ... The purity and concentration of each plasmid were assessed with a NanoDrop™ Lite Spectrophotometer (Invitrogen). Two days before transfection ...
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bioRxiv - Cell Biology 2023Quote: ... plasmids and siRNA were transfected in mammalian cells at 70% confluency using Lipofectamine 3000 (Invitrogen, #L3000001). PLK4 siRNA and CEP152 siRNA oligonucleotides (Merck ...
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bioRxiv - Molecular Biology 2023Quote: ... Gibson assembled plasmids were then transformed into Multishot™ TOP10 Chemically Competent E.coli (Invitrogen™ #C4081201) cells using manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... The amplified DNAs were inserted into the multiple cloning sites of plasmid pCDNA3.1 (+) (Thermo Fisher Scientific). To synthesize RNA ...
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bioRxiv - Cell Biology 2023Quote: ... Subconfluently cells were transfected using 2 µg/ml of plasmid and Lipofectamin 2000 reagent (Life Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... capped RNA was generated from the pToto1101-nsP3-mScarletI plasmid (Invitrogen mMessage mMachine SP6 kit, AM1340) and electroporated into BHK-J cells ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The newly synthesized plasmids were transfected into DH5-alpha competent cells (Thermo Fisher Scientific, MA, USA), and the transfected cells were plated on LB agar plates containing ampicillin for selection ...
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bioRxiv - Genomics 2024Quote: ... GUS control plasmid was provided as part of the LR Clonase II enzyme kit (Invitrogen 11791020). Lentiviral supernatants were collected at 24 and 48 hours and concentrated via spin dialysis ...
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bioRxiv - Molecular Biology 2024Quote: ... HeLa cells were transfected with 500 ng of plasmid DNA using Lipofectamine 2000 (Thermo Fisher Scientific) and following manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... the cloned reporter plasmids and the control pEGFP-N1 were co-transfected by Lipofectamine 3000 (Invitrogen) at a ratio of 3:1 (2.5 μg in total) ...
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bioRxiv - Cancer Biology 2024Quote: ... The plasmid was then transfected into MCF-7 cells using the Lipofectamine LTX (Invitrogen, Carlsbad, CA) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... A CMV expression plasmid encoding Sumo-HRV-CD19-Spytag-Histag was transfected into Expi293F Cells (ThermoFisher) using the ExpiFectamine 293 Transfection Kit (ThermoFisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... Expression plasmids were transfected into the ExpiCHO cell lines according to the manufacturer’s protocol (Thermo Fisher). Supernatant containing monoclonal antibodies were clarified by centrifugation (1,400g ...
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bioRxiv - Cancer Biology 2024Quote: ... A3BWT and A3BE255Q sequences (cDNA plasmids gift from John Maciejowski) were cloned into pDONR221 (Invitrogen 12536017) using the Gateway BP Clonase II Enzyme mix (Invitrogen 11789020) ...
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bioRxiv - Developmental Biology 2024Quote: ... pHLsec-Tsg-TwinStrep and pHLsec-CHRDL2-TwinStrep plasmids were transfected to Expi293-F cells (ThermoFisher Scientific) using the ExpiFectamine 293 Transfection Kit (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... both plasmids containing sgRNAs were transfected into naïve HEK293 cells by Lipofectamine 3000 (Thermo Fisher Scientific) as instructed by the manufacturer ...
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bioRxiv - Cell Biology 2024Quote: ... The plasmid was introduced by heat shock into DH5alpha and extracted with standard Miniprep (#K0503, ThermoFisher). The S71A and S71E mutations were introduced into the pDONR221-gCDC42attb plasmid with the QuickChange II XL Site Directed Mutagenesis Kit (#200521 ...
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bioRxiv - Cell Biology 2024Quote: ... The three plasmids were transfected together into keratinocytes using Lipofectamine LTX with PLUS reagent (Invitrogen #A12621) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... 70%-80% confluent monolayer of cells was transfected with various plasmid constructs using Lipofectamine 2000 (Invitrogen) and Turbofectamine in Opti-MEM (Invitrogen) ...
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bioRxiv - Physiology 2024Quote: ... the pA16-GFP plasmid was linearized by incubation with the restriction enzyme EcI136II (EcoICRI, Thermo Scientific) at 10 U/μ purification using the QIAprep Spin MINI Prep Kit (Qiagen) ...
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bioRxiv - Cancer Biology 2024Quote: ... and pVSV-G (390 ng) packaging plasmids in 5 × 105 HEK293T cells with Lipofectamine 2000 (Invitrogen). The supernatant was collected 40 hours later ...
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bioRxiv - Genomics 2024Quote: ... which was purified using PureLink™ Endotoxin-Free Maxi Plasmid Purification Kit (ThermoFisher Scientific, cat. #A31231) to obtain 2 mg of the final focused STARR-seq plasmid library ...
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bioRxiv - Cell Biology 2024Quote: ... Plasmids were transfected into HEK293T and HeLa cells using jetPEI (Polyplus transfection) or LipofectamineTM 3000 (Invitrogen), and siRNAs were transfected into HeLa cells using LipofectamineTM 2000 ...
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bioRxiv - Neuroscience 2024Quote: ... plasmids were first transformed into One Shot TOP10 Chemically Competent Escherichia coli (Thermo Fisher Scientific; #C404003) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... HepG2 Cells were transfected with the sgRNA plasmid for Hsp90α with Lipofectamine 3000 (Invitrogen, CA, USA). After 48 h ...
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bioRxiv - Microbiology 2024Quote: ... Transfection of the plasmids into HEK293F was performed using polyethyleneimine (PEI) diluted in Opti-MEM (Gibco). After 24 hours ...
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bioRxiv - Bioengineering 2024Quote: ... STAT6 and NOS2 plasmids were co-transfected with psPAX2 and pMD2.G with Lipofectamine 3000 (ThermoFisher) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... Omp25 IVT optimized plasmid was transcribed via T7 polymerase in the presence of RNase inhibitor (Invitrogen) for 1 hour at 37° C ...
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bioRxiv - Bioengineering 2024Quote: ... lentivirus transfer plasmid was constructed from (pLenti6/V5-DEST™ Gateway™ Vector, Thermo Fisher Scientific) as backbone plasmid and entry plasmid (pENTR-H2B-EGFP ...
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bioRxiv - Microbiology 2024Quote: ... DENV RNA was transcribed in vitro from pACYC-DENV2-NGC plasmid using mMessage/mMachine kits (Ambion) and electroporated into BHK-21 cells ...
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bioRxiv - Neuroscience 2024Quote: ... 5.2 μg of the plasmid of interest and 880 µl of Optimem (Gibco, No. 31985-070). Plasmids were mixed ...
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bioRxiv - Microbiology 2024Quote: ... 1µg of plasmid DNA per mL of expi293F cells were complexed with Expifectamine transfection reagent (ThermoFisher) and incubated at room temperature for 20 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... Plasmid concentrations were measured using the ND-1000 Spectrophotometer (NanoDrop, Unity Lab Services, Thermo Fisher Scientific) and the sequences were partially validated using Sanger Sequencing (Eurofins Genomics) ...
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bioRxiv - Systems Biology 2024Quote: ... Plasmids went through an additional step of 5’ dephosphorylation using FastAP Thermosensitive Alkaline Phosphatase (Thermofisher, EF0654) to avoid self ligation ...
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bioRxiv - Immunology 2024Quote: ... 3T3-L1 cells were transfected with one of the above-mentioned plasmids using Lipofectamine 2000 (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: Plasmids of pET28a-phop or pET28a-phop derivative were first transformed into BL21(DE3) (Invitrogen, Inc.). Then ...
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bioRxiv - Neuroscience 2024Quote: ... Concentrations of extracted plasmids were quantified using a Nanodrop 1000 (Thermo Fisher Scientific; Waltham, MA, USA). Efemp1 sgRNAs targeting exons were designed based on the efemp1 genome DNA sequence (gDNA ...
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bioRxiv - Microbiology 2020Quote: ... falciparum blood-stage parasites were cultured in 2.5% hematocrit human RBC in malaria culture media (MCM) comprising of 10.43g RPMI 1640 powder (Gibco), 25ml 1M HEPES (Gibco) ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with Bovine Pituitary Extract (25 mg) and EGF Human Recombinant (2.5 μg) (Gibco, Life Technologies Limited, USA). This medium was established through previous successes in obtaining cell lines from primary tissue [26] ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with Bovine Pituitary Extract (25 mg) and EGF Human Recombinant (2.5 μg) (Gibco, Life Technologies Limited, USA). This medium was established through previous successes in obtaining cell lines from primary tissue [26] ...
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bioRxiv - Cancer Biology 2021Quote: ... The silencer select siRNA targeting human ERK3 and the Silencer non-targeting Control #1 were purchased from Ambion.
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bioRxiv - Cancer Biology 2021Quote: Cells (1-1.5×10^6) were incubated with FACS sample buffer (DPBS, 0.5% FBS) and human FC Block (Invitrogen) for 20 minutes on ice followed by incubation at 4°C for 30 minutes with the following - antibodies ...
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bioRxiv - Cell Biology 2020Quote: Normal human fibroblast cell line (AG08469, Coriell Institute for Medical Research) was maintained in Minimum Essential Medium (Gibco) and supplemented with 15% FBS (HyClone) ...
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bioRxiv - Cell Biology 2020Quote: Silencer Select siRNAs against human SNX4 and VPS35, and nontargeting control “scrambled” siRNA (predesigned, 4,390,844) were purchased from Ambion® (Thermo Fisher Scientific) ...