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Citations for Thermo Fisher :
6101 - 6150 of 10000+ citations for 6H 1 3 Dioxolo 4 5 g 1 benzopyran 6 one 7 8 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... mutations were inserted in fragment 8 (Supplementary Table 4) using the GENEART site-directed mutagenesis system (Thermo Fisher Scientific, Cat# A13312) according to the manufacturer’s protocol with the primers listed in Supplementary Table 5 ...
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bioRxiv - Systems Biology 2022Quote: Cells were cultured and transfected (as described above) for CLSM experiments on 4 or 8-well Lab-Tek™ chambered cover glass slides (ThermoFisher). Before imaging ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The PCR products were analyzed by using an 8 capillary ABI 3500 DNA Genetic Analyzer with POP-4 polymer (Life Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We extracted RNA from sets of 8 adult males (2~4 day old) in triplicate from each RNAi cross using TRIzol (Catalog# 15596-026, Invitrogen, USA), treated ~2 μg RNA with RNase-free DNase I (Catalog# M0303S NEW ENGLAND Biolabs ...
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bioRxiv - Immunology 2020Quote: 7575 naïve CD4 cells were isolated from lymph nodes of CCT8T+/+ and CCT8T−/− mice at 4-8 weeks of age by FACS sorting directly into lysis buffer (RIPA buffer (ThermoFisher Scientific) + 4 % IGEPAL CA-360 (Merck) ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were cleared by centrifugation (19 000 rpm for 45 min at 4°C in a Fiberlite F21-8×50y (Thermo Scientific). Supernatant was incubated with 5 ml Glutathione Sepharose 4B beads slurry (Cytiva ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were cultured to confluence and passaged 4 times before plating to 8-chamber slides (Thermo Fisher Scientific Cat#177445) coated with Matrigel (Thermo Fisher Scientific Cat# CB-40230 or Corning Cat# 356230 ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were cleared by centrifugation at 18,000 rpm for 45 min at 4°C in a SORVAL RC6+ centrifuge with an F21S-8×50Y rotor (Thermo Scientific). The supernatant was filtered through an 0.45 µm filter and loaded onto a pre-equilibrated 5 ml His-Trap HP column (Cytiva) ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates were sonicated twice for 30 s and then cleared by centrifugation at 18,000 rpm for 45 min at 4°C in a SORVAL RC6+ centrifuge with an F21S-8×50Y rotor (Thermo Scientific). The supernatant was collected and incubated with pre-equilibrated Amylose beads (Biolabs ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates were cleared by centrifugation at 18,000 rpm for 45 min at 4°C in a SORVAL RC6+ centrifuge with an F21S-8×50Y rotor (Thermo Scientific). The supernatant was collected and incubated with pre-equilibrated Glutathione Sepharose 4B beads (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates were cleared by centrifugation at 18,000 rpm for 45 min at 4°C in a SORVAL RC6+ centrifuge with an F21S-8×50Y rotor (Thermo Scientific). The supernatant was collected and incubated with pre-equilibrated Glutathione Sepharose 4B beads (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were cleared by centrifugation at 18,000 rpm for 45 min at 4°C in a SORVAL RC6+ centrifuge with an F21S-8×50Y rotor (Thermo Scientific). The supernatant was filtered through an 0.45 µm filter and loaded onto a pre-equilibrated 5 ml His-Trap HP column (Cytiva) ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were cleared by centrifugation at 18,000 rpm for 45 min at 4°C in a SORVAL RC6+ centrifuge with an F21S-8×50Y rotor (Thermo Scientific). The supernatant was collected and incubated with pre-equilibrated Glutathione Sepharose 4B beads (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were cleared by centrifugation at 18,000 rpm for 45 min at 4°C in a SORVAL RC6+ centrifuge with an F21S-8×50Y rotor (Thermo Scientific). The supernatant was collected and incubated with pre-equilibrated Glutathione Sepharose 4B beads (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were cleared by centrifugation at 18,000 rpm for 45 min at 4°C in a SORVAL RC6+ centrifuge with an F21S-8×50Y rotor (Thermo Scientific). The supernatant was collected and incubated with pre-equilibrated Glutathione Sepharose 4B beads (GE Healthcare ...
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bioRxiv - Biophysics 2024Quote: ... 100 mM NaCl and centrifuged again at 40,000 x g for 20 min at 4 °C using a Sorvall LYNX 6000 centrifuge and a A27-8 x 50 fixed angle rotor (both Thermo Scientific). Inclusion bodies were then solubilized in 50 mM Tris pH 8.0 ...
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bioRxiv - Biophysics 2024Quote: ... 50 mM DTT for 30 min at room temperature and non-solubilized material was removed via centrifugation at 40,000 x g for 20 min at 4 °C using a Sorvall LYNX 6000 centrifuge and a A27-8 x 50 fixed angle rotor (both Thermo Scientific).
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bioRxiv - Biophysics 2024Quote: ... The lysate was subsequently cleared by centrifugation at 40,000 x g for 60 min at 4 °C using a Sorvall LYNX 6000 centrifuge and a A27-8 x 50 fixed angle rotor (both Thermo Scientific). The cleared lysate was then loaded onto Ni-NTA (QIAGEN ...
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bioRxiv - Biophysics 2024Quote: ... The lysate was subsequently cleared by centrifugation at 40,000 x g for 30 min at 4 °C using a Sorvall LYNX 6000 centrifuge and a A27-8 x 50 fixed angle rotor (both Thermo Scientific). The pellet was resuspended in 20 mM Tris pH 8.0 ...
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bioRxiv - Biophysics 2024Quote: ... The lysate was subsequently cleared by centrifugation at 87,207 x g for 1 hr at 4 °C using a Sorvall LYNX 6000 centrifuge and a A27-8 x 50 fixed angle rotor (both Thermo Scientific). All subsequent purification steps were carried out in a cold room at 4 °C in analogy to SKP1-FBXO22.
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bioRxiv - Biophysics 2024Quote: ... The lysate was subsequently cleared by centrifugation at 87,207 x g for 1 hr at 4 °C using a Sorvall LYNX 6000 centrifuge and a A27-8 x 50 fixed angle rotor (both Thermo Scientific). All subsequent purification steps were carried out in a cold room at 4 °C ...
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bioRxiv - Biophysics 2024Quote: ... The lysate was subsequently cleared by centrifugation at 87,207 x g for 2 hrs at 4 °C using a Sorvall LYNX 6000 centrifuge and a A27-8 x 50 fixed angle rotor (both Thermo Scientific). The clear lysate was loaded onto a 5 mL StrepTrapHP column using an ÄKTA pure chromatography system (Cytiva) ...
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bioRxiv - Genomics 2019Quote: 2 μl of TAF as well as conventional ChIP library was used for checking enrichment with various primer pairs (listed in Table 1) on Applied Biosystem ViiA™ 7 real time machine using SYBR green reagent (Life technologies, Cat No-4367659).
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bioRxiv - Systems Biology 2023Quote: ... After two freeze-thaw cycles in liquid nitrogen and defrost at 60°C 5 mg ml-1 lysozyme (Carl Roth) and 0.5 μl RNase A (Thermo Scientific™, 0.1 μg ml-1 final) was added and incubated for 1 h at 37°C ...
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bioRxiv - Physiology 2019Quote: ... Hearts were immersed for one day in a chemical mixture obtained by mixing 25 wt% urea (U16–3, Fisher Scientific Hampton, NH, USA), 25 wt% N,N,N0,N0-tetrakis(2-hydroxypropyl ...
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bioRxiv - Molecular Biology 2020Quote: ... We used 1.6 μg of c-Myc monoclonal antibody and 50 μL PanMouse IgG magnetic beads or 1 µl of anti-Flag antibody and 30 µl Protein G magnetic beads (Thermo Scientific). For Cdc5-TAP ChIP ...
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bioRxiv - Developmental Biology 2021Quote: ... The gel was fixed with 40% methanol and 10% acetic acid and then stained for 1 hour using colloidal coomassie dye G-250 (Gel Code Blue Stain, Thermo Scientific). Each lane from the gel was cut and placed in 1.5 ml tubes ...
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bioRxiv - Microbiology 2022Quote: ... Ligated reaction mixture was heated to 95°C for 10 min for inactivation and mixed with 1 volume of 2x formamide orange G dye before purifying with 10% denaturing urea-TBE acrylamide gel (Thermo Scientific) at 300V for 20 min ...
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bioRxiv - Molecular Biology 2020Quote: ... the rest of the clarified lysate was then incubated with 25 uL Protein G magnetic beads (Pierce) coupled with 1 μL mouse anti-V5 (Life Technologies) antibody for 2 hours at 4°C with rotation ...
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bioRxiv - Cell Biology 2019Quote: ... The gel was fixed with 40% methanol/10% acetic acid and then stained for 1 h using colloidal coomassie dye G-250 (Gel Code Blue Stain Reagent, Thermo Scientific). After in-gel digestion ...
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bioRxiv - Biochemistry 2019Quote: Protein concentration in g ⋅ L−1 of human serum was calculated using PierceTM Bicinchoninic Acid (BCA) Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... DAPI was applied at 1:10,000 dilution for 15 minutes and sections were mounted on slides with Fluoromount-G (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2021Quote: ... and infected at a MOI of 1 with the VSV-ΔG-Fluc-2A-GFP/VSV-G virus diluted in 10mL Opti-MEM (Life Technologies). The cells were incubated 1 hour at 37C with 5% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... cushion at 20,000 x g for 1 h in a Sorvall WX-90 ultracentrifuge and SureSpin 630 rotor (Thermo Fisher Scientific). Viral stock titers were titered on MRC-5 (TB40/EFb-eGFP ...
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bioRxiv - Bioengineering 2021Quote: MDCK.2 wild type canine kidney epithelial cells (ATCC) were cultured in customized media consisting of low-glucose (1 g/L) DMEM with phenol red (Gibco, USA), 1 g/L sodium bicarbonate ...
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bioRxiv - Microbiology 2020Quote: ... and 6.25 μg mL−1 were isolated by the hot acid-phenol method27 followed by treatment with Turbo DNA-free™ kit (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... and cultured in minimal medium containing 1 part of DMEM (4.5 g/L D-Glucose, Pyruvate, L-Glutamine; Life Technologies; 41966029): 1 part DMEM/F12 (Life Technologies ...
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bioRxiv - Neuroscience 2022Quote: ... placed into 1.5 ml tubes and incubated with recombinant sNEGR1 (10 µg/ml) or bovine serum albumin (10 µg/ml) diluted in Neurobasal A supplemented with gentamycin/amphotericin (1:500, ThermoFisher Scientific) for 24 h at room temperature with constant agitation ...
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bioRxiv - Immunology 2020Quote: ... The solution was allowed to mix at room temperature for 1 h and then added to recently washed and dried magnetic protein G Dynabeads (Thermo Fisher). The mixture was incubated for 1.5 h and resin was separated from the supernatant magnetically ...
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bioRxiv - Developmental Biology 2020Quote: ... The gel was fixed with 40% Methanol and 10% acetic acid and then stained for 1 hour using colloidal coomassie dye G-250 (Gel Code Blue Stain, Thermo Scientific). Each lane from the gel was cut and placed in 1.5 ml tubes ...
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bioRxiv - Microbiology 2020Quote: ... at OD600 = 1.1±0.1 by centrifugation (4000×g for 10 min) and resuspended in 700 μl of Tri-reagent (Invitrogen, ThermoFisher, Loughborough, UK), vortexed vigorously ...
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bioRxiv - Developmental Biology 2020Quote: ... The gel was fixed with 40% methanol/10% acetic acid and then stained for 1 h using colloidal coomassie dye G-250 (Gel Code Blue Stain Reagent, Thermo Scientific). After in-gel digestion (Noordstra et al. ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 10 μl of stock alizarin red solution (1 g Alizarin Red S from Acros Organics, 400460100 in 100 ml 0.5% KOH) were added per 1 ml of 1% KOH ...
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bioRxiv - Biochemistry 2021Quote: ... 1 g of wild caught Sockeye salmon homogenate was weighted and placed in 50 mL Falcon tubes (Fisher Scientific, cat # LS4541). In order to assess the recovery ...
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bioRxiv - Bioengineering 2022Quote: ... the suspending fluid was 0.6% w/v or 0.7% w/v methyl cellulose (Thermo Scientific, molecular weight 454.513 g mol−1) in phosphate buffered saline (PBS ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: 5 × 104 short-term CAR T cells (determined as VSV-G+ and CAR+ cells) were co-cultured with 1×104 NALM6 cells prelabeled with CellTrace® Violet (CTV) (ThermoFisher) in 200 μL 4Cell® Nutri-T medium (Sartorius ...
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bioRxiv - Neuroscience 2023Quote: ... at RT for 10 min and a final wash with 0.01x Buffer B for 1 min the coverslips were mounted in Fluoromount G (Thermo Fisher Scientific) and imaged at the Imaging Facility of the Max Planck Institute for Biological Intelligence ...
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bioRxiv - Microbiology 2022Quote: ... Labeled secondary antibody incubation was performed for 1 hour at room temperature after which the slides were mounted using Fluoromount-G Mounting Medium with DAPI counterstain (Invitrogen, #00495952). A Nikon Eclipse Ti2-S-HW microscope was used for all imaging and NIS-Elements AR 5.11.00 (Nikon ...
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bioRxiv - Neuroscience 2022Quote: ... injection of wheat germ agglutinin (WGA) -Alexa Fluor™ 594 conjugate (0.004 mg/g BW of 1% WGA-Alexa Fluor™ 594, Invitrogen) via the femoral artery catheter and imaged with the laser tuned to 850 nm ...
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bioRxiv - Cell Biology 2022Quote: ... HRP-conjugated goat anti-rat IgG (catalog #62-9520) and Zysorbin-G (catalog #10-1051-1) were from Invitrogen (Carlsbad, CA). The mouse IgG1 (catalog #401407) ...