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Citations for Thermo Fisher :
6251 - 6300 of 10000+ citations for 6H 1 3 Dioxolo 4 5 g 1 benzopyran 6 one 7 8 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... 3 hours for E14.5 and 4 hours for E18.5 tissues and then blocked in CAS Block (ThermoFisher) for at least two hours ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were subcultured every 3-4 days by washing with Dulbecco’s phosphate buffered saline (DPBS, GibcoTM, ThermoFisher) and detached with trypsin (GibcoTM ...
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bioRxiv - Immunology 2022Quote: ... Epithelium was then collected by centrifugation (3 min, 300g, 4 ºC) and digested by TrypLE express (Gibco) for 1 minute at 37ºC and vortexed ...
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bioRxiv - Microbiology 2022Quote: ... adding 3 mL HPLC grade methanol and 2 mL HPLC grade chloroform (C297-4, Thermo Fisher Scientific), then shaking at 22°C for 1 hr ...
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bioRxiv - Synthetic Biology 2024Quote: ... Samples were stained 3 days at 4°C with phalloidin (0.66 µM, Alexa Fluor 488, Thermofisher, #A12379) and DAPI (0.3 mg/ml ...
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bioRxiv - Genomics 2023Quote: ... and CRISPRmap library plasmid (2:3:4 ratio by mass) using Lipofectamine 3000 (Thermo Fisher Scientific L3000001) in lentiviral packaging medium (Opti-MEM (Thermo Fisher Scientific 31-985-070 ...
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bioRxiv - Microbiology 2023Quote: ... N-(3-triethylammoniumpropyl)-4-(p-diethylaminophenyl-hexatrienyl) pyridinium dibromide (FM4-64) (Thermo Fisher Scientific, Waltham, MA, USA) as previously described (9) ...
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bioRxiv - Cell Biology 2023Quote: ... hESCs were passaged at 70-80% confluency every 3-4 days into Geltrex coated dishes (Life Technologies) using gentle cell dissociation reagent (STEMCELL-Technologies) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 1,1’-dioctadecyl-3,3,3’,3’-tetramethylindodicarbocyanine, 4-chlorobenzenesulfonate salt (DiD, catalog number: D7757) was purchased from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Immunology 2023Quote: ... for 3-4 days to OD 0.6 in tryptone soya broth (Oxoid) supplemented with 10% FCS (Gibco) and the antibiotics above ...
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bioRxiv - Cell Biology 2024Quote: ... ells were stained with 3 mM Fluo-4-AM for 25 minutes in DMEM (Thermo Fisher, 10567014). After two washes with DPBS ...
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bioRxiv - Developmental Biology 2019Quote: ... blastocysts were collected from uterine horns and put on culture for 3-6 days in ES derivation medium composed of GlutaMAX/DMEM (Gibco, 31966), 15% FBS (Biowest) ...
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bioRxiv - Systems Biology 2019Quote: A mate-pair library with 3-6 kb fragments was prepared from pure genomic DNA using Ion TrueMate Library Reagents (Life Technologies). The Ion PGM™ template OT2 400 kit (Life Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... Embedded tissues were cut into 3-6 µm sections using a paraffin microtome (Leitz) and placed on SuperFrost® Plus slides (Menzel Gläser, Thermo Scientific). Deparaffination and antigen retrieval was done as described previously (Link et al. ...
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bioRxiv - Immunology 2022Quote: ... and the wells were washed five times with wash buffer before the addition of 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid (ABTS, #37615, Thermo Fisher Scientific). Optical density was read 40 min later at 405 nm using a plate reader (SpectraMax i3 ...
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bioRxiv - Cell Biology 2021Quote: ... The same amount of protein lysate was aliquoted into different PCR tubes and simultaneously subjected to 6 different temperatures (37, 41, 45, 49, 53, 57 °C) for 3 min in the Veriti Thermal Cycler (Applied Biosystems). After 3 min cooling on ice ...
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bioRxiv - Cell Biology 2022Quote: ... the cells were expanded to 6 × 106/mL the day before transfection and were then diluted to 3 × 106/mL by ExpiCHO™ expression medium (Gibco). Plasmids were diluted to 20 μg/mL by cold OptiPRO medium (Gibco ...
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bioRxiv - Evolutionary Biology 2022Quote: ... was added to cDNA and the DNA was denatured at 95°C for 3 min prior to loading in Novex 6% TBE-urea gel (Thermo Fisher). Samples were separated for 45 min at 180 V ...
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bioRxiv - Neuroscience 2022Quote: ... 3.5 cm) containing 30 larvae and a plastic-covered stirrer (magnetic stir bar micro PTFE 6 mm x 3 mm; Fisher Scientific) were placed on a magnetic stir plate (Variomag Poly 15 stirrer plate ...
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bioRxiv - Cancer Biology 2024Quote: ... The samples were subjected to 6 rounds of 10 sec sonication each at power 3 in a 550 Sonic Dismembrator (Fisher Scientific). Between each round of sonication ...
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bioRxiv - Neuroscience 2024Quote: Determination of sIL-6R was performed in the FN of NL and 21 dpi of aged WT and GFAP-IL6Tg (n=3-6/experimental group) using a precoated mouse ELISA kit (EMIL6RA, Fisher Scientific) and following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: HEK-293T cells were seeded on 6-well culture plates at 3 x 106 cells/well and grown in Dulbecco modified Eagle medium (DMEM; Thermo Fisher Scientific ...
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Changes in cell morphology and function induced by NRAS Q61R mutation in lymphatic endothelial cellsbioRxiv - Cell Biology 2023Quote: ... NM_002524.5) with a 3’ terminal 6 × His-tag was chemically synthesized and cloned into the pFastBac1 vector (Invitrogen, Carlsbad, California, USA). cDNA of NRASQ61R was cloned into the pLVSIN-EF1α-AcGFP-C1 vector (Takara Bio Inc. ...
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bioRxiv - Cancer Biology 2023Quote: ... 3 µL of primers (final concentration 200 nM) and 6 µL of Power SYBR Green PCR Master Mix (Thermo Fisher Scientific). The amplification conditions included an initial incubation for 2 min at 50°C ...
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bioRxiv - Neuroscience 2024Quote: ... differentiated OLs at 6 DIV (‘OL 3 days’) were transfected with plasmids using Lipofectamine LTX with Plus Reagent (Thermo Fisher Scientific) according to the manufacturer’s recommendations ...
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bioRxiv - Evolutionary Biology 2020Quote: ISH and dFISH were performed as previously described (3, 7, 62) with addition of T1 RNAse treatment (2 u/µl, Thermo Fisher Scientific, Waltham, MA, USA) after probe washing in embryos older than 4 dpf to reduce background staining ...
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bioRxiv - Bioengineering 2021Quote: Human lung fibroblasts (hTERT T1015, abmgood) were used between passages 7-12 and culture medium was changed every 2-3 days (Gibco Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10 v/v% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2021Quote: ... To obtain Mfs purified monocytes were plated on 24 well plates 3 × 105 cells/well and cultured for 7 days in Macrophage-SFM (Gibco, Life Technologies, Grand Island, NY, USA) with recombinant human GM-CSF (Miltenyi Biotech ...
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bioRxiv - Cell Biology 2020Quote: ... Ovaries were washed with PBST for 15 minutes before staining nuclei with TO-PRO-3 (Life technologies, 1:1000) in PBST for 15 minutes ...
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bioRxiv - Evolutionary Biology 2021Quote: ... PBS was then washed 3 times and 1 times CellMask™ orange Actin Tracking Stain (Thermofisher; Catalogue number: A57244) and allowed to incubate for another 15 mins at RTP to stain for F-actin ...
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bioRxiv - Synthetic Biology 2022Quote: ... and used in a ligation reaction with the inserts (3:1 ratio) using T4 DNA ligase (Thermo Fisher, EL0011), at 22°C for 4–6 h ...
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bioRxiv - Neuroscience 2021Quote: ... tissue was incubated in 3% NDS-PB-TX primary antisera to FR (1:1000, Invitrogen #A6397, made in rabbit), FS (1:500 ...
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bioRxiv - Molecular Biology 2021Quote: ... counted and total RNA was recovered from 3×106 cells using 1 ml of Trizol (Invitrogen, Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... counted and total RNA was recovered from 3×106 cells using 1 ml of Trizol (Invitrogen, Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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The tumour microenvironment shapes dendritic cell plasticity in a human organotypic melanoma culturebioRxiv - Immunology 2019Quote: ... Adult human dermal fibroblasts were purchased by ATCC and maintained in Fibroblast medium (3:1 DMEM: Ham’s F12 Nutrient Mixture, Gibco) supplemented with 10% FCS ...
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bioRxiv - Neuroscience 2019Quote: ... washed 3× in PBS and incubated with Alexa-conjugated anti-mouse secondary antibody (1:500, Alexa Fluor 647, Invitrogen) for 1 h at room temperature ...
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bioRxiv - Biochemistry 2019Quote: ... ATS-bound peptides were washed with 50 mM ammonium bicarbonate (3×) and eluted with 1 mM TCEP (Thermo Scientific) for 30 min at 37°C with constant rotation ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The soluble fraction was then loaded onto 1 mL or 3 mL HisPur Ni-NTA Spin Columns (Thermo Scientific) and His-tagged protein was purified according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2019Quote: ... Ligation reactions were performed by using a 3:1 insert/vector ratio with T4 DNA Ligase (Thermo-Fisher Scientific) and transformed into chemically competent Escherichia coli DH5α cells ...
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bioRxiv - Neuroscience 2020Quote: Glial primary cultures were established from cerebral cortices of C57BL/6J or Sfrp1-/- 1-3 days-old pups dissected in Ca2+/Mg2+-free Hank’s Balanced Salt Solution (HBSS, Invitrogen) and processed following standard procedures [79] ...
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bioRxiv - Immunology 2021Quote: ... kidneys from infected mice were harvested at 3 days post infection andplaced for 1 hour in RNAlater solution (Invitrogen). Kidneys were homogenized in Lysing Matrix C tubes (MPbio) ...
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bioRxiv - Physiology 2020Quote: ... Membranes were washed 3 times with TBST and incubated with anti-mouse IgG secondary antibody (A16017, Thermofisher, 1:10,000) or anti-rabbit IgG secondary antibody (A16035 ...
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bioRxiv - Neuroscience 2020Quote: ... The next day slices were washed 3× in 0.3% PBS-T then incubated in Goat ∝ Chicken:488 secondary antibody (1:1,000, Invitrogen, A32931) for 2 h at room temperature ...
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bioRxiv - Systems Biology 2020Quote: ... Pellets were then resuspended in 500µl of solution containing a ratio of 3:1 of water to LDS Sample Buffer (4x) (Invitrogen) with a final concentration of 2mM DTT ...
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bioRxiv - Cancer Biology 2022Quote: ... 3 × 103 Panc-1 cells per 30 µl were pre-labeled with Hoechst 33342 (Thermo Fisher Scientific; Waltham, MA) for 1 hour prior to trypsinization and resuspended in tumor sphere-forming media (Irvine Scientific ...
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bioRxiv - Biophysics 2022Quote: ... and 1% v/v Triton X-100 along with 3 pellets of EDTA-free Pierce Protease Inhibitor (ThermoFisher Scientific), and 0.25 mg/ml hen egg white lysozyme ...
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bioRxiv - Molecular Biology 2022Quote: ... Secondary antibodies were applied for 2 hours in PBS/3% milk powder containing 1 μg/ml Hoechst-33342 (Invitrogen) or DAPI (Roche ...
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bioRxiv - Molecular Biology 2020Quote: The rat insulinoma cells (INS-1, 832/3)54 were cultured in RPMI-1640 medium (Thermo Fisher Scientific, 11875119) supplemented with 10% FBS ...
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bioRxiv - Neuroscience 2021Quote: ... 500 μl of washing buffer was added (Tris-HCL 10 mM, NaCl 10 mM, MgCl2 3 mM, Tween-20 0.1%, BSA 1%, Invitrogen™ RNAseout™ recombinant ribonuclease inhibitor 0.04 U/μL ...
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bioRxiv - Physiology 2022Quote: ... The nucleic acids present in the pituitary gland cells were visualised with TOTO-3 (1:2000, Thermo Fisher Scientific). Sections were incubated with TOTO-3 for 15 min on an agitated surface ...