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Citations for Thermo Fisher :
6351 - 6400 of 10000+ citations for 6H 1 3 Dioxolo 4 5 g 1 benzopyran 6 one 7 8 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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SHARPIN enhances ferroptosis in synovial sarcoma cells via NF-κB- and PRMT5-mediated PGC1α reductionbioRxiv - Cancer Biology 2023Quote: Yamato cell lines were seeded into 6-well plates and incubated with 5 µM BODIPY 581/591 C11 (Invitrogen) for 30 minutes at 37°C ...
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bioRxiv - Immunology 2023Quote: ... The paraffin blocks were sliced into 5 to 6 μm sections using a Microm HM 325 microtome (Thermo Scientific), and the sections were stained with Hematoxylin and Eosin (H&E) ...
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bioRxiv - Cell Biology 2024Quote: ... An overnight incubation with specific antibodies (P16, IL-6 or phospho-H2AX (S139) and cTNT (Invitrogen, #MA-5-12960)) was followed by 2 hours of incubation with secondary antibody conjugated with Alexa Fluor 488 ...
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bioRxiv - Neuroscience 2024Quote: ... cells were switched to neural induction media (DMEM/F12 and Neurobasal medium at 1:1 ratio, 1% B27, 0.5% N2, 1% PenStrep, 1% Glutamax; ThermoFisher Scientific) supplemented with ascorbic acid (0.1 mM ...
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bioRxiv - Molecular Biology 2019Quote: ... To initiate transcription 4 µL of 5 X first strand buffer (Invitrogen, Cat No. 1889832) and 1 µL each of 0.1 M DTT (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... A 4’-5-diamidina-2-phenylindole (DAPI)-containing mounting medium was used (ThermoFisher, cat. 62248). Images were acquired using a spectral Zeiss LSM800 confocal microscope or Confocal Zeiss LSM980 - AiryScan and analyzed with Fiji software (version 2.0.0) ...
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bioRxiv - Cell Biology 2021Quote: ... A 4’-5-diamidina-2-phenylindole (DAPI)-containing mounting medium was used (ThermoFisher, cat. 62248). Images were acquired using a spectral Zeiss LSM800 confocal microscope and analyzed with Fiji software (version 2.0.0) ...
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bioRxiv - Biochemistry 2021Quote: ... then fixed with 4% paraformaldehyde containing 5 µg/ml Hoechst 33342 stain (Thermo Fisher, #62249) for 10 min at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated overnight (4° C) with 5 µg of Cx36 (Thermofisher, 37-4600, RRID: AB_2533320) or NMDAR1 (Thermofisher ...
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bioRxiv - Microbiology 2020Quote: ... 5-10 ul of each sample was loaded on 4-12% Bis-Tris gels (Thermofisher).
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bioRxiv - Neuroscience 2022Quote: ... for 5 min and loaded onto 4%–12% Nupage Bis-Tris gels (Novex/Life Technologies). Proteins were transferred using the iBlot2 (Life Technologies ...
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bioRxiv - Microbiology 2022Quote: ... 4-5 ×106 P25 TCR-Tg CD4+ T cells (CD45.1) were labeled with CellTrace (Invitrogen) and adoptively transferred by tail vein injection in 100 µL of sterile PBS 24 hours (h ...
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bioRxiv - Neuroscience 2024Quote: ... and cells were split every 4–5 days using 0.5 mM EDTA (Invitrogen, 15575-020). hiPS cell clones/lines were also generated using retroviruses or non-integrating episomal vectors from fibroblasts (Bhaduri et al. ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were passaged every 4-5 days by treatment with StemPro Accutase (ThermoFisher, Cat#A1110501), stored in liquid nitrogen ...
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bioRxiv - Microbiology 2023Quote: ... the 11.5 μL RNA were supplemented with 4 μL 5× first strand buffer (ThermoFisher Scientific), 1 μL 10 μM reverse primer ...
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bioRxiv - Cancer Biology 2023Quote: ... for 5 min Samples were run down a Novex 4–12% Tris-glycine gel (Invitrogen) and transferred onto a nitrocellulose membrane (Bio-Rad ...
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bioRxiv - Bioengineering 2024Quote: ... adding 5 mM GMP and 4 U/µL RNaseOUTTM recombinant ribonuclease inhibitor (Thermo Fisher Scientific) to the transcription reaction ...
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bioRxiv - Immunology 2022Quote: To analyze cell morphology 5×104 cells were resuspended in 100mL PBS and cytocentrifuged onto slides at 350g for 5 minutes (Thermo Scientific, Cytospin 4) before fixation in methanol for 15 minutes and staining with May-Grünwald’s (VWR Chemicals ...
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bioRxiv - Neuroscience 2019Quote: ... fixed slices were washed with PBS 4 times for 10 minutes and transferred to a solution containing AlexaFluor® Streptavidin 488 (Invitrogen, 1:1000 or 1:500, Catalog #: S32354) and Neurotrace 640/660 (Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: ... 5 µL of each SF was diluted 7-fold in water and used for a Bradford protein assay (Thermo Fisher Scientific, Rockford, IL).
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bioRxiv - Biophysics 2022Quote: African green monkey kidney cells (COS-7) were cultured at 37°C and 5 % CO2 in DMEM medium containing glutamax (Gibco No. 31966-047), 10 % fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2022Quote: Fast-growing human embryonic kidney 293T cells (HEK293FT) were maintained in DMEM 10% FBS (v/v) and passaged at 5-7 day intervals with TrypleExpress (Invitrogen GIBCO, Paisley, UK,) to detach the cells ...
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bioRxiv - Neuroscience 2023Quote: ... 15-20 pooled day 30 neurospheres and 5-7 pooled day 60 organoids using TRIzol (Invitrogen; Thermo Fisher Scientific Inc., Waltham, MA, USA) and Direct-Zol RNA Mini Prep (Zymo Research ...
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bioRxiv - Cell Biology 2021Quote: ... Dissociated cells were collected by centrifugation (200 g, 3 min at 25 °C) and resuspended in complete medium comprising neurobasal medium (Gibco, 21103) supplemented with 2% B-27 plus (Gibco A3582801) ...
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bioRxiv - Neuroscience 2020Quote: ... washed 3 more times in PBS for 10 minutes each and mounted on cover slides with Floromount G (Thermo Fisher Scientific). Slips were imaged an Olympus VS120 slide scanning microscope.
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bioRxiv - Genomics 2022Quote: The following steps were conducted in 0.2 mL tubes (Axygen Biosciences): 3 μL of D2 buffer from a REPLI-g Single Cell Kit (Thermo Fisher Scientific) was added to 4 μL of droplet suspension ...
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bioRxiv - Biophysics 2019Quote: ... 2.5 µg of freshly SEC-purified MBP-RIC-3-His6 (bait) was added to 15 µL of HisPurTM Cobalt resin (Thermo Scientific) equilibrated with and re-suspended in 200 µL of binding buffer (Buffer A ...
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bioRxiv - Biochemistry 2020Quote: The gel was run for 2-3 cm and stained with colloidal coomassie dye G-250 (Gel Code Blue Stain Reagent, Thermo Scientific) after which each lane was cut out ...
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bioRxiv - Cell Biology 2021Quote: ... plasmid DNA (2 μg of each plasmid) and FuGENE (3 μL FuGENE/μg DNA) were mixed thoroughly in 100 μL Opti-MEM (Thermo Fisher) and incubated at room temperature for 15 min ...
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bioRxiv - Genomics 2021Quote: ... 5 × 106 cells were plated in 10 cm dishes and co-transfected with 3 μg VSV-G plasmid and 9 μg of the respective construct Lipofectamine LTX (Thermo Fisher) the following day ...
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bioRxiv - Immunology 2021Quote: ... Coverslips were washed 3 times and mounted on slides using Fluoromount-G™ mounting medium with DAPI (Thermofisher; 00-4959-52). Images were acquired on an SP5 confocal microscope and analysed using ImageJ software.
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bioRxiv - Evolutionary Biology 2022Quote: ... A poly(G)-tail was added at the 3’ end of the RNA according to a Poly(A) Polymerase protocol (Thermo Scientific), using GTP instead of ATP ...
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bioRxiv - Molecular Biology 2022Quote: ... Antibodies (3 μg for ChIP-qPCR and 9 μg for ChIP-seq) were coupled to protein G dynabeads (Thermo Fisher Scientific) for 6 hours at 4°C and then incubated with fragmented chromatin over night at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting membrane pellet was resuspended in buffer M10 at 3 mL/g (of starting mitochondria) and the protein concentration was determined using a BCA assay (Pierce Thermo Fisher). The resuspended membranes were stored at 10 mg/mL of total protein at -80°C in a final glycerol concentration of 30% (v/v ...
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bioRxiv - Immunology 2022Quote: ... Cells were counted again and resuspended in 3 × 106 cells/mL before adding CC4F-AM (LiveBLAzer FRET-B/G Loading Kit with CCF4-AM, ThermoFisher Scientific). Cells were incubated for 1 hour ...
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bioRxiv - Neuroscience 2024Quote: ... hPSCs were centrifuged at 300 x g for 3 min and resuspended in 500 µL accutase (ThermoFisher Scientific, #00-4555-56) for 3 min at RT ...
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bioRxiv - Neuroscience 2024Quote: ... either for 2-3 cm or ran completely and stained with colloidal coomassie dye G-250 (Gel Code Blue Stain Reagent, Thermo Scientific). The lane was cut into bands ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Sections were washed again in PBST 3 times and were mounted using Fluoromount-G with DAPI (Thermo Fisher Scientific, Waltham, MA). They were imaged on a BZ-X800 Analyzer Keyence fluorescence microscope (Keyence ...
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bioRxiv - Molecular Biology 2024Quote: ... After centrifugation at 300 x g for 3 minutes the pellet containing embryonic neurons was resuspended in cortical neurons growth media: Neurobasal (Gibco 21103049), B27 Supplement (Gibco ...
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bioRxiv - Plant Biology 2023Quote: ... The resulting supernatant was mixed with 0.25% G-250 Sample Additive and loaded on a NativePAGE 3-12% Bis-Tris gel (BN1001BOX, Invitrogen, CA, USA) for electrophoresis.
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bioRxiv - Immunology 2023Quote: ... slides were washed 3 times with 0.2% Tween-20 in PBS and then mounted in Fluoromount G (Invitrogen, 00-4958-02). For signal amplification of CD163 and multiplexing with primary antibodies from the same species (Rabbit ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were washed twice with 1X PBS and stained with either DAPI (4’,6-diamidino-2-phenylindole, from Life Technologies), for the visualization of the nucleus ...
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bioRxiv - Microbiology 2021Quote: ... Slides were washed for 3 × 5 mins in PBS after each antibody step and in the penultimate wash 2μg/ml 4’,6-diamidino-2-phenylindole (DAPI) (Molecular Probes) was added ...
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bioRxiv - Immunology 2019Quote: Skin samples were collected from the cheek of mice at the indicated time points with a 4- or 6-mm biopsy punch into cold RPMI 1640 medium (RPMI; Gibco) and minced into smaller pieces with surgical scissors ...
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bioRxiv - Microbiology 2019Quote: ... at room temperature for 60 min and washed again with PBS-T and then incubated with DAPI (4’, 6-diamidino-2-phenylindole) (Invitrogen) at room temperature for 20 min ...
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bioRxiv - Cell Biology 2019Quote: ... Nuclei were stained with 10.9 μM 4⍰6-diamidino-2-phenylindole (DAPI) and cover glasses were mounted in ProlongGold (Life Technologies). Images were collected on an inverted epifluorescence microscope (Nikon TE2000E ...
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bioRxiv - Neuroscience 2020Quote: ... The coverslips were then mounted on slides using prolong Gold mounting medium with DAPI (4’,6-diamidino-2-phenylindole) (Invitrogen). RGCs immunohistochemistry was performed as described earlier 36–39 using SOX2 ...
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bioRxiv - Neuroscience 2021Quote: ... were harvested from male and female adult WT C57BL/6 mice and immersed in a 4% paraformaldehyde solution (PFA) made in phosphate-buffered saline (PBS, Cat #AAJ19943K2, ThermoFisher) and stored at 4 °C overnight prior to transfer to 15% sucrose solution in PBS for an additional 24 h ...
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bioRxiv - Neuroscience 2021Quote: ... in PBS for two hours at room temperature followed by 0.5 μg/mL of 4′,6-diamidino-2-phenylindole (DAPI) (ThermoFisher Scientific) in PBS for five minutes at room temperature prior to mounting.
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bioRxiv - Neuroscience 2020Quote: ... the sections were counterstained with DAPI (4’,6-diamidino-2-phenylinodole) and mounted onto glass slides (Superfrost Plus; Fisher Scientific) with Vectashield mounting medium (H-1200 ...