Labshake search
Citations for Thermo Fisher :
6151 - 6200 of 10000+ citations for 6H 1 3 Dioxolo 4 5 g 1 benzopyran 6 one 7 8 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2023Quote: ... Secondary antibodies used are: ECL anti-mouse immunoglobulin G (IgG) horseradish peroxidase linked whole antibody (1/10 000; Fisher Scientific; NXA931V) for anti-MAP1B ...
-
bioRxiv - Microbiology 2023Quote: ... F and G in Figure 1 titer was determined using a PA-HPLC analysis using a POROS A 20 μm column (Applied Biosystems) on an Agilent 1260 system ...
-
bioRxiv - Microbiology 2023Quote: ... and goat anti-mouse IgG (G-21040) secondary antibodies conjugated to horseradish peroxidase (HRP) were used at 1:5,000 (ThermoFisher Scientific Invitrogen). SuperSignal West Pico Chemiluminescent Substrate was used to detect HRP on immunoblots per manufacturer instructions (ThermoFisher Scientific) ...
-
bioRxiv - Cancer Biology 2023Quote: ... Horseradish peroxidase– conjugated secondary antibodies, goat anti-mouse immunoglobulin G (IgG) (# 32230), and goat anti-rabbit IgG (# 32260, 1:5000) were purchased from Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2023Quote: ... All cell lines had their culture medium supplemented with 1% penicillin and streptomycin (10,000 g/ml; Life Technologies, Carlsbad, CA USA), as well as 0.1% gentamicin (50 mg/ml ...
-
bioRxiv - Biophysics 2023Quote: ... and 1 mM CHAPS) by centrifugation at 1000x g for 2 minutes with a micro-spin desalting column (Thermo Scientific Zeba) at 4 °C ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cells were washed 3 times with 1X PBS solution and incubated for 1 hour at room temperature with the following secondary antibodies: anti-Ig G mouse conjugated with Alexa488 (Molecular probes), anti-Ig G rat conjugated with Alexa555 (Life technologies ...
-
bioRxiv - Plant Biology 2023Quote: ... patens gametophores were grown on KNOP ME agar plates (12 g l−1 purified agar; Oxoid, Thermo Scientific, Waltham, MA, USA). Light intensity in growth cabinets was set to 70-100 µmol photons m−2 s−1 and 16:8 h light/dark cycle at 22 °C ...
-
bioRxiv - Immunology 2023Quote: HeLa-H2B-eGFP and MRC5 (ATCC CCL-171) were maintained as monolayers in Dulbecco’s modified Eagle’s medium (DMEM) (1 g/L glucose) (Gibco, Billings, MT, USA) supplemented with 10% heat-inactivated FBS and Pen–Strep.
-
bioRxiv - Cell Biology 2023Quote: ... or 0.5 µg rtTA-puro and 1 µg of PiggyBac (for control rtTA cells) in 100 µl Opti-MEM solution (31985062, Life Technologies) containing 5 µl of X-tremeGENE 9 transfection reagent (XTG9-RO Roche ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... we identified a SNP present only in lineage Media1 (Chromosome 1, position 4527201, G/A) and designed a Custom Taqman SNP Genotyping Assay (Thermofisher, UK) that can detect this specific SNP using a FAM-labelled probe for this SNP and a VIC-labelled probe for the reference base ...
-
bioRxiv - Molecular Biology 2023Quote: ... cells were collected by centrifugation for 5 min at 500 g, re-suspended in ice-cold Sorting Medium (1% Fetal Bovine Serum in PBS, 0.25mg/mL Fungizone (Thermo Fisher Scientific), 0.25μg∕mL/10μg∕mL Amphotericin B/Gentamicin (GIBCO) ...
-
bioRxiv - Biochemistry 2024Quote: ... Parasites were cultured in HMI-9 medium supplemented with 10% inactivated FBS and 2.5 µg ml-1 G-418 (Life Technologies Europe) at 37°C in a humidified atmosphere containing 5% CO2 (G-418 was included for maintenance of TetR and T7RNAP) ...
-
bioRxiv - Immunology 2024Quote: ... The epithelial solution was spun down at 1000 g for 2 minutes and then resuspended in 1 mL of TrypLE express enzyme (ThermoFisher #12604013) for 5 minutes at 37°C ...
-
bioRxiv - Immunology 2024Quote: ... Replication-competent VSV-G pseudotyped HIV-1 virus was prepared by co-transfecting 293FT cells with the respective HIV-1 molecular clone and VSV-G expression plasmid using Lipofectamine 3000 reagent (Life Technologies). Transfection media was replaced with fresh antibiotic free DMEM media 8 h post-transfection ...
-
bioRxiv - Microbiology 2020Quote: ... A 25 μl RT-PCR for the viral E gene was carried out using 5 μl of extracted RNA template using the AgPath-ID One-Step RT-PCR (Applied Biosystems). Samples were processed in duplicate ...
-
bioRxiv - Developmental Biology 2019Quote: ... 1 μL of this reaction was used for reverse transcription and preamplification of cDNA in a 5 μL one-step reaction using the RT enzyme from Taqman RNA-to-Ct (ThermoFisher, #4392938) and the Taqman Preamp Master Mix (ThermoFisher ...
-
bioRxiv - Microbiology 2020Quote: One milliliter of monomeric capsid (5 mg/ml) was dialyzed in SnakeSkin dialysis tubing (10K MWCO, Thermo Scientific, Waltham, MA, USA) using a buffer that was high in salt and contained a reducing agent (Buffer 1 ...
-
bioRxiv - Neuroscience 2024Quote: ... and the primers listed in Extended Data Table 5 were annealed at 61degC on the Step One Plus Real-Time PCR System (Life Technologies). Fold-changes were calculated using the ΔΔCt method.
-
bioRxiv - Neuroscience 2023Quote: ... Sections were then washed 3× for 5 min each in vehicle and then incubated in vehicle containing IgG-subclass-specific secondary Abs for one hour (Thermo Fisher). Sections were then washed 3× for 5 min each in PB and mounted with Prolong Gold (Thermo Fisher cat#P36930 ...
-
bioRxiv - Bioengineering 2023Quote: ... mRNA was co-transcriptionally capped using m7(3’OMeG)(5’)ppp(5’)(2’OMeA)pG capping reagent (Hongene Biotech) in a “one-pot” reaction followed by digestion with DNase I (Thermo Fisher). Linear mRNA was purified using Dynabeads MyOne Carboxylic Acid beads (Thermo Fisher).
-
bioRxiv - Cancer Biology 2024Quote: ... and each treatment condition was labeled with one channel of TMT-11plex reagent (5 µl of 25 µg/µl reagent per reaction, prepared in dry acetonitrile, Thermo Scientific) for 1 h at 23 °C ...
-
bioRxiv - Biochemistry 2023Quote: ... followed by one to two weeks of selection with fresh medium containing 200-500 μg/ml Hygromycin (Invitrogen, ant-hg-5) or 7.5 μg/ml Blasticidin (Invitrogen ...
-
bioRxiv - Biochemistry 2023Quote: ... The temperature was increased from 5 °C to 95 °C over 2 hours and readings taken using a One Step Plus RT-PCR (Applied Biosystems).
-
bioRxiv - Biophysics 2021Quote: ... Secondary antibodies were goat anti-mouse-Star red (Abberior, 2-0002-011-2) and goat anti-rabbit Star 580 (Abberior 2-0012-0050-8) (Life Technologies, 1:100).
-
bioRxiv - Cell Biology 2019Quote: ... five day 1 adult males were dissected in 35μL pH 7.8 SM buffer (50mM HEPES, 50mM NaCl, 25mM KCl, 5mM CaCl2, 1mM MgSO4, 1mg/ml BSA) with JC-1(Thermo Fisher Scientific T3168) added to 10μM final concentration ...
-
bioRxiv - Molecular Biology 2021Quote: ... pombe cells were mixed in an 8:1 ratio and total RNA was extracted using reagents from RiboPure yeast kit (Thermo Fisher Scientific #AM1926) using the following volumes ...
-
bioRxiv - Immunology 2021Quote: ... The grid was then blotted for 3.0 s with a blot force of −1 at 8 °C and 100% humidity and plunge-frozen in liquid ethane using a Vitrobot (Thermo Fisher Scientific, USA). Cryo-EM data sets were collected at a 300 kV Titan Krios microscope (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2023Quote: ... The GMEM/KSR medium was consisted of 1 × Glasgow’s MEM (GMEM; Wako, 078-05525) supplemented with 8% KnockOut Serum Replacement (KSR; Thermo Fisher Scientific, 10828-028). In the initial optimization experiments ...
-
bioRxiv - Neuroscience 2023Quote: ... The primary antibodies used were 1:30 rat anti-N-cadherin (Developmental Studies Hybridoma Bank, DN-EX #8,) and 1:200 rabbit anti-lucifer yellow (Molecular Probes, A-5750). The secondary antibodies used were 1:500 goat anti-rabbit with Alexa fluor 633 (Molecular Probes ...
-
bioRxiv - Developmental Biology 2023Quote: ... Dissected tails were then placed in individual wells of 8-well glass-bottomed dishes (Lab-Tek Chambered #1 Borosilicate Coverglass System 155411, Thermo Scientific Nunc, USA) with 200µl of Gibco CO2 independent medium (Thermo Fisher #18045054).
-
bioRxiv - Cell Biology 2023Quote: ... qPCRs were performed using cDNA (1:8 dilution) and the ORA™ SEE qPCR Green ROX L kit (highQu) on a PikoReal 96 machine (Thermo Fisher Scientific). GraphPad Prism was used for graphics/statistics ...
-
bioRxiv - Bioengineering 2024Quote: ... It was further baked at 95°C for 8 min and developed by submerging in SU-8 developer (Microchem SU-8 developer, Fisher Scientific) for at least 15 min ...
-
bioRxiv - Bioengineering 2023Quote: ... It was further baked at 95°C for 8 min and developed by submerging in SU-8 developer (Microchem SU-8 developer, Fisher Scientific) for at least 15 min ...
-
bioRxiv - Microbiology 2021Quote: A one-step phosphopeptide IMAC enrichment procedure was performed using a 4 × 50 mm ProPac IMAC-10 analytical column (Thermo Fisher Scientific). For the JY cells an equivalent of 5 mg of protein was used for one IMAC purification while for the C ...
-
bioRxiv - Immunology 2023Quote: ... 2,000 cells were resuspended in 1µL of ECM mixture and plated on one end of a well on a 4 well Labtek plate (Thermo Scientific, cat#154917). 0.8ng of CXCL9 (R&D systems ...
-
bioRxiv - Pathology 2023Quote: ... cryotubes containing one million progenitors were thawed and seeded at 23,500 cells/cm² on Lab-Tek™ 4-well chamber slides (Thermofisher Cat# 154526PK) coated with collagen I (1:60 dilution) ...
-
bioRxiv - Pathology 2023Quote: ... cryotubes containing one million progenitors were thawed and seeded at 23,500 cells/cm² on Lab-Tek™ 4-well chamber slides (Thermofisher Cat# 154526PK) coated with collagen I (1:60 dilution) ...
-
bioRxiv - Biophysics 2019Quote: ... ZO-1 (1:100, ThermoFisher), vimentin (1:100 ...
-
bioRxiv - Bioengineering 2020Quote: ... 1:1,(GIBCO, Life Technologies) medium supplemented with 10% fetal bovine serum(GIBCO) ...
-
bioRxiv - Bioengineering 2020Quote: ... 1:1,(GIBCO, Life Technologies) medium supplemented with 10% fetal bovine serum(GIBCO) ...
-
bioRxiv - Neuroscience 2019Quote: ... 1:1 Neurobasal (ThermoFisher; 21103049) with 1× Glutamax ...
-
bioRxiv - Cell Biology 2023Quote: ... 1% HEPES (1 M, Gibco), 1% L-glutamine (200 mM ...
-
bioRxiv - Cell Biology 2022Quote: ... 1% HEPES 1 M (Gibco), 1% Sodium Pyruvate 100 mM (Gibco) ...
-
bioRxiv - Cell Biology 2023Quote: ... ZO-1 (1:100, ThermoFisher), E-Cadherin (1:100 ...
-
bioRxiv - Zoology 2021Quote: ... and with NanoDrop One (ThermoFisher) with target OD 260/280 and OD 260/230 ratios of 1.8 and 2.0-2.2 ...
-
bioRxiv - Microbiology 2021Quote: ... One-step Ultra TMB (ThermoFisher) was then used for detection ...
-
bioRxiv - Immunology 2021Quote: ... one labeled with AlexaFluor647 (Invitrogen), were used in this panel in order to increase specificity of the detection of SARS-CoV-2-specific B cells ...
-
bioRxiv - Immunology 2021Quote: ... one labeled with AlexaFluor488 (Invitrogen), one labeled with AlexaFluor647 (Invitrogen) ...
-
bioRxiv - Genomics 2023Quote: ... spectrophotometric (Nanodrop One, Fisher Scientific) determination of purity ...