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Citations for Thermo Fisher :
5951 - 6000 of 10000+ citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: Cells were transiently transfected with plasmid constructs for hTERT and hTR using Lipofectamine 2000 (Life Technologies) or the jetPrime reagent (Polyplus transfection) ...
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bioRxiv - Microbiology 2020Quote: ... Plasmids were isolated after assembly and electroporation into MEGAX DH10B T1R Electrocomp Cells (Thermo Fisher Scientific) with the QIAprep Spin Miniprep Kit (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... Transfection of oligonucleotides and plasmids in pseudovirus infection models were performed using Lipofectamine™ 3000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... and the BCR-encoding B-cell specific plasmid pRRL EuB29 gl2-1261 IgGTM.BCR.GFP.WPRE53 (2.4 μg) using lipofectamine 2000 (Invitrogen). Prior to lentiviral production ...
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bioRxiv - Immunology 2021Quote: ... Plasmids encoding Spike-2P and HexaPro 61 were transiently transfected in FreeStyle 293 cells (Thermo Fisher) using Turbo293 (SpeedBiosystems ...
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bioRxiv - Molecular Biology 2020Quote: ... The pU6-2-gRNA plasmid was cut with BBSI-HF restriction enzyme (Fisher Scientific Catalog# NC1222468) in 1X CutSmart Buffer and de-phosphorylated with Calf Intestinal Alkaline Phosphatase (Fisher# 50811712) ...
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bioRxiv - Microbiology 2021Quote: ... cells were transfected with desired plasmid DNA using Lipofectamine 2000 transfection reagent (Life Technologies, California, USA) at 1:1 ratio ...
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bioRxiv - Immunology 2021Quote: HeLa cells were transiently transfected with the plasmid encoding HexaPro using lipofectamine 3000 (Invitrogen, Cat#L3000008). Cells were fixed with 4% PFA and were incubated with either a polyclonal antibody against the SARS-CoV-2 RBD protein (Sino Biological ...
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bioRxiv - Immunology 2020Quote: ... The plasmids were sequenced using a BigDye(tm) Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, USA). The BigDye(tm ...
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bioRxiv - Cell Biology 2021Quote: ... HeLa WT cells were transfected with 1 μg/ml plasmid using TurboFect transfection reagent (Thermo Fisher) according to manufacturer’s instruction and selected with 2 μg/ml puromycin for four days ...
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bioRxiv - Neuroscience 2022Quote: ... Cas9 was transcribed from linearized parental pX330 plasmid using mMESSAGE mMACHINE T7 kit (Ambion/Thermo Fisher), the resulting mRNA was polyadenylated using Poly(A ...
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bioRxiv - Neuroscience 2022Quote: ... Cas9 was transcribed from linearized parental pX330 plasmid using mMESSAGE mMACHINE T7 kit (Ambion/Thermo Fisher), the resulting mRNA was polyadenylated using Poly(A ...
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bioRxiv - Cancer Biology 2022Quote: ... Lentivirus was packaged as previously described (52) in 293FT using 3rd generation packaging plasmids (Virapower, Invitrogen) with polyethyleneimene (PEI ...
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bioRxiv - Bioengineering 2022Quote: ... or HindIII-linearized plasmid pT7_PhiC31o as the template and mMESSAGE mMACHINE™ T7 Transcription Kit (Invitrogen). The former vector contains a phiC31 coding sequence (phiC31)(Bischof et al.,2007) ...
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bioRxiv - Biochemistry 2022Quote: ... DENV replicon RNAs were transcribed from XbaI-linearized plasmids (MEGAscript T7 Transcription kit, Thermo Fisher Scientific), purified (RNeasy MinElute columns ...
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bioRxiv - Cancer Biology 2022Quote: ... addgene plasmid #80475) was digested with MluI followed by a LR gateway clonase reaction (ThermoFisher, 11791020) for 18 hours ...
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bioRxiv - Cell Biology 2022Quote: ... The three plasmids were mixed and diluted to 50 µL in Opti-MEM Medium (Gibco 31985062). 1.25 µL Lipofectamine 2000 was diluted to 50 µL in Opti-MEM Medium ...
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bioRxiv - Cell Biology 2022Quote: ... the plasmid was linearized and transcribed in vitro using mMessage mMachine T7 kit (Ambion, Cat# AM1344) according to manufacturer’s protocol.
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bioRxiv - Genomics 2022Quote: ... Plasmids and siRNAs were co-delivered by reverse transfection using Lipofectamine 2000 (Thermo Fisher Scientific 11668) for Cos-7 cells and Lipofectamine 3000 for HepG2 cells ...
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bioRxiv - Genomics 2022Quote: ... cells were transfected with 1 µg of plasmid DNA using Lipofectamine LTX with Plus Reagent (ThermoFisher) following the manufacturer’s recommendations ...
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bioRxiv - Immunology 2022Quote: ... using the Neon Transfection System (100 µg/ml plasmid, 1005 V, 35 ms, 2 pulses; ThermoFisher) and single-cell clones were obtained by limited dilution ...
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bioRxiv - Immunology 2022Quote: Plasmids were transformed into One Shot™ Stbl3™ Chemically Competent bacteria (Thermo Fisher Scientific #C737303). Single colonies were selected and inoculated in LB Broth ...
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bioRxiv - Microbiology 2022Quote: ... The plasmid was transiently transfected into Expi293F cells using ExpiFectamine™ 293 Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: ... cells were cotransfected with 9 μg vector plasmid and 4.5 μg RD114 with Lipofectamine 2000 (Invitrogen) in Opti-MEM media (Gibco) ...
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bioRxiv - Molecular Biology 2023Quote: ... Fluc-FSECoV-2-Rluc (–1) reporter plasmid DNAs were transfected using Lipofectamine 2000 (Thermo Fisher, 11668030). After 48 hours ...
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bioRxiv - Plant Biology 2022Quote: ... and further subcloned into the destination plasmid pMDC107 (Curtis and Grossniklaus, 2003) by LR reaction (Invitrogen). The constructs were introduced into Agrobacterium tumefaciens strain GV3101 and were then used to transform corresponding single mutant or WT (e.g. ...
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bioRxiv - Microbiology 2023Quote: ... Correct plasmid structure was confirmed using restriction analysis using the FastDigest KpnI enzyme (Thermo Fisher Scientific) according to the manufacturer’s instructions and by EZseq Sanger sequencing (Macrogen ...
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bioRxiv - Immunology 2022Quote: The plasmids encoding 46.1-182.1v/61.1-182.1 were transfected into Expi293 cells using ExpiFectamine 293 (ThermoFisher) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... For Gibson Assembly pSRK plasmid was PCR-amplified with primers pSRK_GA_F and pSRK_GA_R and treated with DpnI restriction endonuclease (ThermoFisher).
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bioRxiv - Molecular Biology 2022Quote: ... The plasmid containing this locus was then transfected into HEK-293 TRex Flp-In cells (ThermoFisher) along with a pOG44 plasmid encoding a Flp-recombinase using Turbofectin according to manufacturer’s protocols ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were transfected with 0.5-1.0 μg plasmid DNA plus 1.0 μL Lipofectamine 2000 (Life Technologies) according to the manufacturers’ instructions and cultured overnight (12-16 h ...
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bioRxiv - Microbiology 2022Quote: ... cells were transfected with the desired plasmids using Lipofectamine 2000 transfection reagent (Thermo Scientific, Massachusetts, USA), at 1:1 ratio ...
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bioRxiv - Immunology 2022Quote: ... The plasmid was transfected into Expi293 cells following the Expi293 expression system kit manufacturer’s instructions (ThermoFisher). Five days after transfection ...
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bioRxiv - Cell Biology 2022Quote: ... Sequence-verified plasmids were then transfected into MRC5 shMUS81 cells by the Neon nucleofector (Life technologies). To generate shBRCA2 inducible cell lines ...
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bioRxiv - Neuroscience 2024Quote: ... The concentration and quality of the linearised plasmid were confirmed using NanoDrop Spectrophotometer (Thermo Fisher Scientific) and gel electrophoresis ...
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bioRxiv - Neuroscience 2024Quote: ... 2 µg of plasmid DNA was mixed with 4 µL of Lipofectamine 2000 (ThermoFisher Scientific #11668019) in 300 µL of Opti-MEM without any additives ...
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bioRxiv - Immunology 2024Quote: ... capped RNA was generated from the pToto1101-nsP3-mScarletI plasmid (Invitrogen mMessage mMachine SP6 kit, AM1340) and electroporated into BHK-J cells ...
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bioRxiv - Microbiology 2024Quote: ... The digested PCR products and backbone plasmid were ligated using T4 DNA ligase (Thermo Fisher Scientific). The mixture was transformed into CaCl2-competent E ...
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bioRxiv - Microbiology 2024Quote: ... IBDV VP3 FL protein was subcloned from plasmid pcDNAVP3 FL into the pFastBacHTb vector (Gibco BRL), which incorporates a 6xHis tag at the N-terminal of the recombinant protein ...
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Bilateral regulation of EGFR activity and local PI dynamics observed with superresolution microscopybioRxiv - Cell Biology 2024Quote: ... HeLa cells were transfected with the resultant plasmid using Lipofectamine 3000 (Thermo Fisher Scientific, Waltham, MA). Single colonies were picked after selection with 0.3 μg/ml puromycin for 3 days ...
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bioRxiv - Cancer Biology 2024Quote: ... plasmid containing a sgRNA targeting APC (5‘-GGCACTCAAAACGCTTTTGA-3‘) using lipofectamin-2000 (Thermo Fisher Scientific, 11668027). WT organoids were grown in WENR medium and APC truncated organoids were grown in ENR medium and are later refererred to as mCol WT and mCol APC ...
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bioRxiv - Cell Biology 2024Quote: ... progeny of this cross were injected with the homology arm carrying pJET1.2 plasmid (Thermo Fisher Scientific). Transgenic candidates were identified by gene specific PCR and confirmed by sequencing of the insertion sites ...
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bioRxiv - Cancer Biology 2024Quote: ... Plasmids and Lipofectamine 2000 reagent were diluted in Opti-MEM medium (31985-070, Thermo Fisher Scientific) and incubated for 5 min at room temperature ...
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bioRxiv - Immunology 2024Quote: ... and the entire set of 8 plasmids was used to transfect 293FT cells (Thermo Fisher Scientific) using Lipofectamine 2000 CD (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... DNA and plasmid concentrations were measured using Quant-iT dsDNA Broad-Range (BR) Assay Kit (Invitrogen). Polymerase chain reactions (PCR ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 1.25 µg plasmid DNA was mixed in Opti-MEM I reduced serum media (Thermo Fisher 11058021) to a final volume of 62.5 µl ...
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bioRxiv - Immunology 2024Quote: ... The following day PlatE cells were transfected with pBMN-plasmids using a Lipofectamin 3000 kit (Invitrogen) according to the manufactures protocol ...
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bioRxiv - Biochemistry 2024Quote: ... 1 μg of purified bacmid plasmid DNA was mixed with 8 μL of Cellfectin II (ThermoFisher) at room temperature to transfect 0.9×106 Sf9 cells per well in a 6-well tissue culture plate in a semi-humid environment ...
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bioRxiv - Cell Biology 2023Quote: ... Subconfluently cells were transfected using 2 µg/ml of plasmid and Lipofectamin 2000 reagent (Life Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... A549 cells were transiently transfected with plasmids using Lipofectamine 3000 (Thermo Fisher Scientific, Les Ulis, France) according to the manufacturer’s instructions.