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Citations for Thermo Fisher :
5851 - 5900 of 10000+ citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... Cells were transfected for 5 hours with 500 ng CD31 plasmid and 0.3% Lipofectamine 2000 (Invitrogen). After 48 hr selection with 5 μg/ml blasticidin began ...
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bioRxiv - Immunology 2019Quote: ... Heavy and light chain plasmids were co-transfected into 293T or ExpiCHO cells (Life Technologies A29133) for antibody expression.
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bioRxiv - Genomics 2020Quote: ... 2 µL of plasmid library were transformed per 50 µL of electrocompetent cells (ElectroMAX Stabl4, Invitrogen) using a Gene Pulser II device (Bio-Rad) ...
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bioRxiv - Cancer Biology 2019Quote: ... specific amounts of viral target and packaging plasmids were mixed with Opti-MEM™ (Thermo Scientific). Next ...
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bioRxiv - Genetics 2019Quote: ... The plasmid was used in a transformation into Subcloning Efficiency DH5α Competent Cells (Invitrogen, Carlsbad, CA). The plasmid was isolated with Zyppy Plasmid Miniprep Kit (Zymoresearch ...
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bioRxiv - Genetics 2019Quote: ... Ligated plasmids were then cloned into DH5α Max Efficiency cells (Thermo Fisher Scientific, Waltham, MA, USA). Two colonies were picked for each plasmid ...
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Cdc42 promotes epithelial morphogenesis by coupling Par-complex and Crumbs recruitment via Par6-aPKCbioRxiv - Cell Biology 2019Quote: ... was used to generate plasmids GST::Cdc42N17 and GST::Cdc42N17using the Gateway™ Cloning System (Invitrogen). The GST::Crbıntra and GST::CrbıntraΔERUconstructs were provided by E ...
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bioRxiv - Genetics 2019Quote: ... Each TALENs plasmid was linearized with NotI and transcribed using the mMESSAGE mMACHINE T7 Kit (Ambion). Polyadenylation of resulting mRNAs was performed using the Poly(A ...
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bioRxiv - Genetics 2021Quote: ... 500,000 cells were transfected with 2.5 µg of each plasmid using Lipofectamine 3000 (Thermo Fisher Scientific). Forty eight hours after transection ...
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bioRxiv - Immunology 2021Quote: ... cells were transfected with 600ng VZV ORF or control expression plasmids using 3μl Lipofectamine 3000 (Invitrogen) per well ...
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bioRxiv - Immunology 2021Quote: 293T cells were transfected with full-length envelope expressing plasmid using lipofectamine2000 (ThermoFisher, catalog no 11668027) as per the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were transfected with CRISPR/Cas9 plasmid containing gRNA using the Neon Transfection System (Invitrogen MPK5000) following standard protocols ...
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bioRxiv - Biochemistry 2020Quote: ... and subsequently purified using the PureLink Quick Plasmid Miniprep Kit (Invitrogen by Life Technologies, Löhne, Germany), and sequenced by the Australian Genome Research Facility (Sydney ...
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bioRxiv - Biochemistry 2020Quote: ... and subsequently purified using the PureLink Quick Plasmid Miniprep Kit (Invitrogen by Life Technologies, Löhne, Germany), and sequenced by the Australian Genome Research Facility (Sydney ...
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bioRxiv - Bioengineering 2021Quote: ... using 200 ng total plasmid and 0.4 μl of commercial transfection reagent Lipofectamine 2000 (Thermo Fisher). Specifically ...
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bioRxiv - Bioengineering 2021Quote: Both Nefmut/SARS-CoV-2-based fusion proteins were cloned into the pVAX1 plasmid (Thermo Fisher) as already described (22) ...
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bioRxiv - Biochemistry 2020Quote: ... plasmids were transformed into BL21 (DE3)-plys competent cells (Life Technologies Corporation, Grand Island, NY, USA) and protein expression was induced at OD600 of 0.5 by adding 0.5mM IPTG ...
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bioRxiv - Microbiology 2021Quote: ... and 25 ng linear pPolI-SARS-CoV2-NLuc-N plasmid using 2μl of Lipofectamine 2000 (Invitrogen) to a DNA:lipofectamine ratio of 1:3 and 100μl Opti-MEM (Gibco) ...
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bioRxiv - Cancer Biology 2020Quote: ... Lentivirus was packaged as previously described [41] in 293FT using 3rd generation packaging plasmids (Virapower, Invitrogen) with polyethyleneimene (PEI ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmid transfections (pEGFPc2-MCM8, pEGFPc2-MCM9, pEGFPc2-MCM9(FR687/8AA) were carried out using LPEI (ThermoFisher) as described.30 The MCM8 or MCM9 gene was cloned into the pIRES2-EGFP using traditional restriction site cloning ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were transiently transfected with the indicated plasmids or siRNAs using Lipofectamine 2000 transfection reagent (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: Plasmids used for plant transformations were generated using the multisite gateway three-fragment strategy (Life Technologies), according the recommendations of the supplier ...
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bioRxiv - Zoology 2021Quote: ... The recombinant plasmid was quantified using a NANO-DROP 2000 spectrophotometer (Thermo Scientific, Waltham, MA, USA). The plasmid copy number was calculated using the equations described by Yun et ...
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bioRxiv - Cell Biology 2021Quote: ... PCR products were subcloned into a plasmid (Zero Blunt TOPO PCR Cloning Kit, Thermo Fisher Scientific) to validate the edited region of positive KO clones by sequencing.
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bioRxiv - Microbiology 2020Quote: ... The fragment was ligated with the pJet1.2 plasmid using the CloneJET PCR Cloning Kit (ThermoFisher Scientific). The recombinant plasmid was linearized using the Ncol restriction enzyme (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... Transient co-transfection of siRNA oligos and DNA plasmids were performed using Lipofectamine 2000 (Thermo Fisher). The following three PINK1 siRNAs were used at a final concentration of 60 pmol/ml each ...
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bioRxiv - Microbiology 2020Quote: Transfection of plasmid DNA or siRNA was performed with a DNA to Lipofectamine® 2000 (ThermoFisher) ratio of 1:2.5 ...
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bioRxiv - Microbiology 2021Quote: ... Purified amplicons were ligated into plasmids using the CloneJET PCR Cloning Kit (Thermo Scientific, Waltham, MA) and then incubated with chemically competent E ...
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bioRxiv - Bioengineering 2019Quote: ... Japan) were transfected with an gRNA expression plasmid using lipofectamine 3000 transfection reagent (Thermo Fisher Scientific). Genomic DNA was extracted 72 hrs post-transfection using a DNA extraction kit (QIAamp DNA Mini kit ...
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bioRxiv - Microbiology 2020Quote: ... Full-length genomic RNA was transcribed from the linearized plasmids using the MegaScript T7 Kit (Ambion). 4µg of transcribed RNA was mixed with DMRIE-C (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... The purified plasmids harboring the genes of interest were transformed into yeast strain INVSc1 (ThermoFisher Scientific) for protein expression and purification ...
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bioRxiv - Molecular Biology 2021Quote: ... Plasmids were Sanger sequenced on a capillary 3130xL Genetic Analyzer (Thermo Fisher Scientific, Waltham, MA, USA) using Sp6 and EBV-rev primers at the sequencing facility at the Department of Biology ...
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bioRxiv - Cell Biology 2021Quote: ... recombinant Cripto protein or transfected with Cripto expression plasmid (pcCripto) using Lipofectamine2000 (Life Technologies, Carlsbad, USA) according to the manufacturer`s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were then transfected with the PX459-Piezo1 plasmid using Lipofectamine LTX (Thermo Fisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmids in HMLE cells were inserted by nucleofection with the Neon Transfection System (Thermo Fisher Scientific), by applying two pulses at 1400V for 20ms ...
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bioRxiv - Biophysics 2020Quote: ... Fusion protein expression plasmids were transfected into (70-90% confluent) cells using Turbofect™ (Thermo Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... plasmid DNA was introduced into HepG2-NTCP cells using Lipofectamine™ 3000 transfection reagent (ThermoFisher Scientific), Huh7 cells were transfected with GenJet™ (SignaGen Laboratories) ...
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bioRxiv - Synthetic Biology 2022Quote: ... All plasmid DNA was quantified using Qubit™ dsDNA BR Assay Kit (Thermo Scientific™ Q32850).
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bioRxiv - Microbiology 2022Quote: All SARS-CoV-2 Spike constructs for pseudotype generation were expressed from plasmid pCDNA3.1 (ThermoFisher #V79020). Codon optimized SARS-CoV-2 Wuhan Spike carrying the D614G amino acid change (Sino Biological #VG40589-UT(D614G) ...
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bioRxiv - Molecular Biology 2022Quote: ... The plasmids (8.0 to 16.0 μg) were mixed with 500 μl of Opti-MEM (Thermo Fisher) and 1.0 μg/μl of PEI Max (32.0 to 64.0 μl ...
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bioRxiv - Biophysics 2022Quote: ... cells were transfected with 0.4µg of indicated MBD-GFP-RFP plasmid for the positive control condition or with 0.2µg of MBD-GFP plasmids by using 0.2µL lipofectamine 2000 (Invitrogen) in optiMEM based on the optimization of the transfection conditions ...
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bioRxiv - Immunology 2022Quote: ... 20 μg of heavy and light chain expression plasmid DNA were diluted in OptiMEM (Life technologies) media containing polyetheleneimine (PEI ...
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bioRxiv - Biophysics 2022Quote: ... The expression plasmids were transiently transfected by using Lipofectamine 3000 (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... and corresponding control plasmid were used to transfect Caco-2 cells using Lipofectamine 2000 (Invitrogen, 11668027) as per the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... Stable cell lines were created by electroporation of plasmids using a Neon Transfection system (Thermo Fisher) according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by DNA purification from 12 dishes of transformants (PureLink HIPure Plasmid Filter Maxiprep Kit, Invitrogen). Lentivirus was prepared by transient transfection of 293FT (Invitrogen ...
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bioRxiv - Developmental Biology 2022Quote: ... mRNA was generated from linearized plasmid using an SP6 mMessage mMachine Transcription Kit (Thermo Fisher Scientific) and column-purified using an RNeasy Mini Kit (Qiagen) ...
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bioRxiv - Cell Biology 2022Quote: ... 1μg DNA of each plasmid construct were added and electroporated using the Neon transfection system (Invitrogen) following the manufacturer’s instructions (1200 volts ...
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bioRxiv - Cell Biology 2022Quote: All plasmids were transcribed into mRNA using the SP6 or T7 mMessage mMachine kit® (Invitrogen) and used for microinjections ...
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bioRxiv - Microbiology 2020Quote: ... the sequence of the plasmid EGFP-N was confirmed by sequencing (Gene Art–Thermo Fisher Scientific).