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Citations for Thermo Fisher :
6001 - 6050 of 10000+ citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... 0.125 µg pAdVantage™ plasmid were added in 250 µl Opti-MEM medium (ThermoFisher Scientific, Gibco). 7.5 µl transfection reagent TransIT-2020 (Mirus Bio ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.125 µg pAdVantage™ plasmid were added in 250 µl Opti-MEM medium (ThermoFisher Scientific, Gibco). 7.5 µl transfection reagent TransIT-2020 (Mirus Bio ...
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bioRxiv - Plant Biology 2023Quote: ... the integrity of the plasmids was checked by digestion with restriction enzyme BglI (Thermo Fisher Scientific). The plasmid DNA was then transformed into Agrobacterium tumefasciens AGL1 strain containing replication helper pSoup (http://www.bract.org/constructs.htm#barley ...
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Engineering synthetic CopT/A-based genetic circuits for miRNA imaging and functional gene regulationbioRxiv - Synthetic Biology 2023Quote: ... the plasmids and miRNA mimics were transfected into cells using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... Plasmid transfection was carried out using the Lipofectamine 3000 Transfection Reagent (Thermo Fisher Scientific, Cat.N. L3000001) and following the manufacturer’s manual ...
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bioRxiv - Cell Biology 2023Quote: ... 2 µg of the epiCRISPR plasmid were transfected into cells by using lipofectamine 3000 (Life Technologies). On day 2 ...
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bioRxiv - Synthetic Biology 2023Quote: ... URA3 containing CRISPR plasmids were removed using the 5-Fluoroorotic Acid (5-FOA) (Thermo Fisher Scientific) counter-selection method with a synthetic defined medium supplemented with 0.1% (w/v ...
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bioRxiv - Biochemistry 2023Quote: ... and HindIII at the 3’-end to facilitate cloning into the pProEx-HTA expression plasmid (Invitrogen) in a way that fuses a coding sequence for a 6xHis tag followed by a TEV cleavage site (i.e. ...
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bioRxiv - Microbiology 2023Quote: ... These plasmids were than co-transfected into 2×106 cells using the Neon Transfection System (Invitrogen) as per the manufacturer’s instructions with the following settings ...
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bioRxiv - Microbiology 2023Quote: ... cells were transfected with 400ng of each plasmid DNA using Lipofectamine 3000 and P3000 Reagent (Invitrogen) according to manufacturer’s instruction.
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bioRxiv - Molecular Biology 2023Quote: ... Transient transfections were performed with 1.5 μg pcDNA5d3-HsPINK1 plasmids using Lipofectamine 3000 Transfection Reagent (Invitrogen), and transfected cells were allowed to grow for 24 h before harvesting ...
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bioRxiv - Biophysics 2023Quote: ... the fragment was ligated into the equally restricted pFastBac1 plasmid using T4 DNA Ligase (Thermo Scientific).
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bioRxiv - Microbiology 2023Quote: ... The plasmid obtained was propagated in competent Escherichia coli (DH10β) cells (Thermo Fisher Scientific, Waltham, USA), resistant to zeocin ...
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bioRxiv - Cell Biology 2023Quote: ... Cultured cells were transfected with siRNAs or plasmids using Lipofectamine 2000 (Invitrogen, CA, USA, #11668-019) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 μg of sgRNA plasmid was transfected to PLC/PRF/5 cells using Lipofectamine 3000 (Invitrogen). After 2 days’ culture in DMEM medium ...
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bioRxiv - Cell Biology 2023Quote: ... Cells at 60-80% confluence were transiently transfected with the indicated plasmids using Lipofectamine 2000 (Invitrogen) according to manufacturer’s protocols ...
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bioRxiv - Bioengineering 2023Quote: General plasmid transfection in HEK 293T cells were performed using Lipofectamine 3000 transfection reagent (Invitrogen, L3000001) according to the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2023Quote: Plasmid CassetteAv2_pBAD contains the CRISPR03 array cloned into the pBAD/Myc–His B backbone (Life Technologies).12 It was used to prepare CRISPR DNA substrates by PCR with primers MMB1Lead40-5 and MMB1crisp3-r1 using Phusion High-Fidelity DNA polymerase according to the manufacturer’s protocol (New England Biolabs or ThermoFisher) ...
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bioRxiv - Microbiology 2023Quote: ... the knockout plasmid pFA094 was generated by PCR amplification with Phusion HF DNA polymerase (Thermo Scientific) of the upstream and downstream flanking regions of recA from B ...
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Engineering TALE-linked deaminases to facilitate precision adenine base editing in mitochondrial DNAbioRxiv - Molecular Biology 2023Quote: ... The cells were transfected with plasmids (total 1 ug) using 1.5 uL of Lipofectamine 2000 (Invitrogen) after 24 h ...
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bioRxiv - Cell Biology 2023Quote: Plasmid transfections were performed according to standard protocols using Lipofectamine 2000 or 3000 (Thermo Fisher Scientific). siRNA transfections were carried out according to standard protocols using DharmaFECT one transfection reagent (Dharmacon ...
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bioRxiv - Biochemistry 2023Quote: ... and 1.5 μg of transgene plasmid was prepared in 37.5 µL of OptiMEM (Thermo Fisher Scientific). This mix was combined with 9 μL of TransIT-LT1 (Mirus ...
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bioRxiv - Cell Biology 2023Quote: ... and 1.64 pmol of transfer plasmid (tet-O-cDNA) in 500 µL of OptiPro SFM (Gibco). Polyethyenimine (PEI ...
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bioRxiv - Cell Biology 2023Quote: ... cells were grown to 75-80% confluency and incubated with plasmid diluted in OptiMem (Gibco, #11058021) and Lipofectamine 3000 + P3000 reagent (ThermoFisher ...
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bioRxiv - Genomics 2022Quote: ... 100 ng of each plasmid was transfected in 293T cells using Lipofectamine 2000 (Thermo Fisher Scientific) following the manufacturer’s instruction ...
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bioRxiv - Genomics 2022Quote: ... The purified plasmid was collected and quantified using a NanoDrop ONE (Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Plant Biology 2023Quote: ... the amplicons were introduced via BP clonase into pDONR207 entry plasmid using Gateway (GW) technology (Invitrogen). Full-length sequences were then subcloned through an LR reaction into pGWB414 and pGWB420 plasmids as previously reported (Barja et al. ...
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bioRxiv - Cancer Biology 2023Quote: ... 70%-80% confluent monolayer of cells was transfected with various plasmid constructs using Lipofectamine 2000 (Invitrogen) and Turbofectamine in Opti-MEM (Invitrogen) ...
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bioRxiv - Biochemistry 2023Quote: ... Plasmid DNA constructs were transiently expressed in HEK293T cells using Lipofectamine 3000 (ThermoFisher Scientific, cat #L3000015). Cells were dosed with CFTR modulators a day after transfection when applicable ...
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bioRxiv - Biochemistry 2023Quote: ... 3A and together with the PiggyBac transposase expression plasmid (hyPBase 51) using Lipofectamine 3000 (Thermo Fisher) in 6-well plates seeded with 400,000 HeLa cells the day before ...
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bioRxiv - Molecular Biology 2023Quote: ... This 4,905-nucleotide gene (Ku70-eGFP-Sun1ΔN) was synthesized and cloned into the pcDNA3.1+ plasmid (Invitrogen) using NheI and XhoI restriction sites ...
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bioRxiv - Immunology 2023Quote: ... The plasmids were transiently transfected into Expi293F cells using ExpiFectamine™ 293 Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... HEK293 cells were transfected with PX459 and pCR-Blunt II TOPO plasmids by Lipofectamine 3000 (Invitrogen) for 72 hr ...
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bioRxiv - Microbiology 2023Quote: HEK-293Ts transfected with the indicated plasmids were lysed in M-PER lysis buffer (Thermo Scientific) supplemented with EDTA-free protease inhibitor (Roche ...
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bioRxiv - Cell Biology 2023Quote: Cells were transiently transfected with the expression vector plasmids using electroporation (Neon Transfection System, Life Technologies) in accordance with the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... followed by PCR and insertion into Zero Blunt TOPO PCR Cloning Plasmid (Thermo Fisher Scientific, 450245) which were transfected and expanded per manufacturer instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... RNA molecules and plasmid were transiently transfected into hESCs using Lipofectamine 3000 (Thermo Fisher Scientific, L3000001) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Ligated plasmid (1 µl) was then transformed into One Shot BL21 Star (DE3) competent cells (Invitrogen) with outgrowth in SOC media for 1 hour at 37°C before plating on LB agar plates containing kanamycin (50 µg/ml) ...
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bioRxiv - Cancer Biology 2023Quote: ... Ligation of this fragment into the pX330 modified plasmid was performed using T4 DNA Ligase (Invitrogen) in an overnight reaction at 16°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were transfected with 10 µg of plasmid DNA (Table S3) and cultured under Puromycin (Gibco) selection (5 µg/mL ...
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bioRxiv - Molecular Biology 2023Quote: ... VSMCs at 70-80% confluence were transfected with indicated plasmids using TransIT-LT1 reagent (Life Technologies). The transfection efficiency of this method was approximately 60%.
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bioRxiv - Developmental Biology 2023Quote: ... Transfection mix containing 0.2 μg of total plasmids and 0.5 μl of Lipofectamine 2000 (ThermoFisher Sciencetific) in 50 μl of OPTI-MEM were added to PGCs in 96 well plate ...
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bioRxiv - Cell Biology 2023Quote: ... plasmids and siRNA were transfected in mammalian cells at 70% confluency using Lipofectamine 3000 (Invitrogen, #L3000001). PLK4 siRNA and CEP152 siRNA oligonucleotides (Merck ...
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bioRxiv - Biophysics 2023Quote: ... the pFastBac plasmid containing either HSET or HSETΔTail was transformed into DH10Bac-competent cells (Gibco, #10361012) using a heat-shock method ...
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bioRxiv - Cell Biology 2023Quote: ... plasmid pYES2-pGAL-FLAG-htt25QP-GFP-URA3 was linearized by restriction enzyme XagI (Thermo Fisher Scientific) to replace huntingtin (htt ...
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bioRxiv - Physiology 2023Quote: ... Concentrations of plasmid DNA were measured with a Nanodrop-1000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Neuroscience 2023Quote: ... Luciferase reporter plasmid DNAs or in vitro transcribed RNAs were transfected by using Lipofectamine 3000 (ThermoFisher) according to the manufacturers’ instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... empty vector control + PPARγ or PPARγ + ZFP407 plasmids using Lipofectamine 3000 (ThermoFisher Scientific, Waltham, MA, USA) according to manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and 1.5 μg of transgene plasmid was prepared in 37.5 µL of OptiMEM (Thermo Fisher Scientific). This mix was combined with 9 μL of TransIT-LT1 (Mirus ...
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bioRxiv - Genetics 2023Quote: pCDNA3.1 phiC31 integrase (Addgene #68310)20,22 plasmid was linearized with BamHI and transcribed using the T7 mMessage mMachine kit (ThermoFisher AM1344). Following IVT ...