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Citations for Thermo Fisher :
5751 - 5800 of 10000+ citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: The release of CXCL10 to the culture medium was detected using Human ProcartaPlex immunoassays (Invitrogen, Vienna, Austria) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2023Quote: Human 293F cells were maintained at 37°C with 5% CO2 in FreeStyle 293 Expression Medium (ThermoFisher) supplemented with penicillin and streptomycin ...
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bioRxiv - Biophysics 2023Quote: ... they were transfected with the S6-tagged human TrkA by means of Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... antigen retrieval techniques were applied human tissue sections using IHC Antigen Retrieval Solution (00-4956-58, Invitrogen). These tissues were stained with Abs against human CD4 pre-labeled with Alexa Fluor 488 using an Ab labeling kit (Molecular Probes ...
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bioRxiv - Bioengineering 2023Quote: ... cells were stained with Hu5F9 or TJC4 and then stained with labeled anti-human secondary antibody (Invitrogen). B6H12 was acquired from Bio X Cell ...
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bioRxiv - Cell Biology 2023Quote: Fibroblasts were isolated from human skin biopsies and were cultured in DMEM/F12 (Gibco, Thermo Fisher Scientific) supplemented with 10% FBS and 1% P/S ...
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bioRxiv - Microbiology 2023Quote: ... gondii parasites were grown in human foreskin fibroblasts (HFFs, ATCC SRC-1041) maintained in DMEM (GIBCO 11965118) supplemented with 3% inactivated fetal calf serum and 10 µg/mL gentamicin (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: Immortalized human retinal pigment epithelial cells (RPE-1hTERT, ATCC) were cultured in Dulbecco’s modified Eagle’s medium (Gibco/Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were activated with 30 U/mL rIL-2 and Dynabeads Human T-activator CD3/CD28 (ThermoFisher) and cultured with the CM of CA-MSCs in the presence or not of CXCR4 Ab (R&D Systems) ...
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bioRxiv - Cell Biology 2023Quote: ... HCC1395 and SF295 human cancer cell lines were grown in RPMI-1640 (Thermo Fisher Scientific, Waltham, MA) medium supplemented with 10% FBS and 50 units/ml of penicillin/streptomycin.
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bioRxiv - Biophysics 2023Quote: ... human IL-2 (Cat# PHC0026) and CellTrace™ CFSE (Cat# C34570) were purchased from ThermoFisher (Waltham, MA). Bovine serum albumin (BSA ...
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bioRxiv - Biochemistry 2023Quote: The human PTH1R (residues 27-502) with G188A and K484R mutations was cloned into pFastBac vector (Invitrogen) with the haemagglutinin signal peptide (HA) ...
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bioRxiv - Neuroscience 2023Quote: ... 5’-CAGAGTCGATCAGTCTGCATATCTCCA-3’) with the dilution protocol of the Phusion Human Specimen Direct PCR Kit (Thermo Scientific). The PCR product was sequenced directly with a nested sequencing primer (5’-GGTGCTCTCCCGGGTACACAA-3’) ...
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bioRxiv - Neuroscience 2023Quote: ... Exogenous mRNA levels of transgene expression human APOE (Hs00171168_m1) commercial TaqMan® primer/probe set (Applied Biosystems). Endogenous mouse Beta-Actin (Mm02619580_g1 ...
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bioRxiv - Immunology 2023Quote: ... Sections were incubated with primary antibodies: rat anti-human Gal3 (Invitrogen #14-5301-82, 1:50 dilution) and mouse anti-human CD68 (Dako #M0814 ...
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bioRxiv - Immunology 2023Quote: The human promyelocytic leukemia HL-60 cell line (ATCC #CCL-240) was maintained in IMDM media (Gibco) with 10% heat-inactivated (HI ...
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bioRxiv - Cell Biology 2023Quote: Human embryonic kidney (HEK) 293T and Vero cells were grown in Dulbecco’s modified Eagle medium (DMEM) (Gibco) supplemented with 10 % fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2023Quote: ... The concentrations of Aβ40 and Aβ42 were determined in brain lysates using ELISA kits according to the manufacturer’s instructions (human Aβ40 and Aβ42 brain ELISA, Invitrogen).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... targeting human SLC22A10 (Assay ID: Hs01397962_m1) and beta actin (Assay ID: Hs99999903_m1) (Applied Biosystems, Foster City, CA). The qRT-PCR reactions were conducted in a 96-well plate ...
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bioRxiv - Immunology 2023Quote: The human myelogenous leukemia cell line K562 was cultured in Roswell Park Memorial Institute (RPMI) media (Gibco) with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2023Quote: Fibroblasts were isolated from human skin biopsies and were cultured in DMEM/F12 (Gibco, Thermo Fisher Scientific) supplemented with 10% FBS and 1% P/S ...
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bioRxiv - Bioengineering 2023Quote: Human foreskin fibroblasts (HFFs; CRL-2429, ATCC) and HFF-derived iPSCs were grown in Geltrex (Gibco, A1413302) coated 12-well plates and were fixed with 3.7% formaldehyde and then blocked with 3% BSA in 1X PBS for 1hr at Room Temperature prior to imaging ...
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bioRxiv - Bioengineering 2023Quote: ... The tissues were then incubated with primary mouse monoclonal anti-human cardiac troponin T (Thermo Fisher Scientific) or mouse monoclonal anti-human CD3 (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... SW837 (human colon cells, ATCC CCL-235) were grown in Leibovitz’s L-15 medium (Gibco #11415-049) with 10% FCS (Hyclone #SH30084.03n ...
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bioRxiv - Cell Biology 2023Quote: Normal human epidermal keratinocytes (Kurabo) from Caucasian neonatal foreskin were cultured with EpiLife medium (Thermo Fisher Scientific) supplemented with 60 µM CaCl2 and HuMedia KG growth factor kit (Kurabo) ...
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bioRxiv - Genomics 2022Quote: ... CD4+ T cells were isolated by magnetic negative selection using Untouched Human CD4+ Isolation Kit (Invitrogen 11346D) per manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2023Quote: ... The medium was ESTEM-HE medium during purification and changed to Human Melanocyte Basal Medium Kit (Gibco) or Melanocyte Growth Basal Medium-4 (Lonza ...
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bioRxiv - Developmental Biology 2023Quote: ... Small interfering RNA was introduced into primary human umbilical vein ECs using the Neon transfection system (ThermoFisher). See Supplementary Table 5 for sources of siRNA ...
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bioRxiv - Microbiology 2023Quote: ... human actin mRNA and EMCV viral RNA was performed mixing the Fast SYBR green Master Mix (ThermoFisher) with cDNA and 1µM of the forward and reverse corresponding primers ...
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bioRxiv - Bioengineering 2023Quote: ... substrates were incubated with PBS solution containing various concentrations of human plasma fibronectin (Thermo Fisher Scientific, US) for 2 h ...
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bioRxiv - Biochemistry 2023Quote: ... followed by incubation with HRP-coupled anti-human IgG (MilleniumScience) before adding the chromogenic substrate TMB (ThermoFisher). Reactions were stopped with 2 M H2SO4 and absorption measured at 450 nm.
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bioRxiv - Genomics 2023Quote: ... KRAB-dCas9 expressing cells were activated with Dynabeads Human T-Activator CD3/CD28 (Thermo Fisher Scientific, 11131D), at 1:1 cell:bead ratio ...
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bioRxiv - Bioengineering 2023Quote: ... the brain sections were incubated for 1 h with goat anti-human IgG Alexa Fluor 647 (Invitrogen) and goat anti-mouse IgG Alexa Fluor 488 (1:500 ...
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bioRxiv - Developmental Biology 2023Quote: ... RNA was isolated from mouse aortas and human aortic SMCs with the PureLink RNA Mini Kit (ThermoFisher). Isolated RNA was reverse transcribed with the iScript cDNA Synthesis Kit (Bio-Rad) ...
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bioRxiv - Microbiology 2023Quote: Human cervix adenocarcinoma epithelial HeLa 229 cells (ATCC CCL-2.1) were cultured in RPMI 1640 medium (Gibco) containing 5% heat-inactivated fetal bovine serum (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... falciparum 3D737 infected erythrocytes were cultured in O positive human erythrocytes in RPMI 1640 media (Gibco, USA) containing 10% heat-inactivated pooled human sera and 5 μg/mL gentamicin (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... version 46) and humans using SEQUEST and Mascot (Version 2.4.1) via Proteome Discoverer v2.2 (Thermo Fisher Scientific). Trypsin was selected as the protease of choice with a maximum of two missed cleavages ...
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bioRxiv - Microbiology 2023Quote: ... THP-1 human monocytes (ATCC) were maintained in Roswell Park Memorial Institute 1640 Medium (RPMI 1640, Gibco) supplemented with 10% FBS ...
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bioRxiv - Physiology 2023Quote: Total RNA was extracted from human lung ECs and mouse lung tissues using TRIZOL reagent kit (Invitrogen). The cDNA was generated using iScript cDNA synthesis kit (170-8891 ...
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bioRxiv - Immunology 2024Quote: ... 1:2000 dilution of secondary Alexa FLUOR-647 GOAT-Anti human IgG [H+L] (Invitrogen; #A-21445) was added to the cells for 30 minutes followed by 3 washes with FACS Buffer ...
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bioRxiv - Systems Biology 2024Quote: Human embryonic kidney (HEK) 293T cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) media (ThermoFisher, UK), supplemented with 10% Heat Inactivated Foetal Bovine Serum (FBS ...
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bioRxiv - Neuroscience 2024Quote: Elastase generated granulin peptides were obtained by incubation of rPGRN with human neutrophil elastase (# RP-77526, Invitrogen). 50 ng/µl PGRN and 80 ng/µl elastase were incubated in 100 mM sodium citrate pH 6.0 overnight at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... hiPS cells were cultured on six-well plates coated with recombinant human vitronectin (VTN–N, Gibco, A14700) in Essential 8 medium (Gibco ...
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bioRxiv - Pathology 2024Quote: Gene expression on human cells and tissue was quantified using TaqManTM assays (Fisher Scientific, Supplementary Table 3) and Luna® Universal qPCR Master Mix (M3003E ...
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bioRxiv - Molecular Biology 2023Quote: ... and S-D614G-N149Q were expressed in human Expi293F cells using the Expi293 Expression System (ThermoFisher Scientific) and purified using a HisTrap FF column (GE Life Sciences)(64 ...
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bioRxiv - Microbiology 2023Quote: ... Secreted antibodies were detected with 1µg/mL goat anti-human IgG (H+L)-Alexa Fluor 594 (Invitrogen), which was added to the cell culture media used to resuspend the antigen-coated beads.
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bioRxiv - Molecular Biology 2023Quote: ... human primary T cells and DG-75 cells were electroporated using the Neon Transfection kit (ThermoFisher Scientific) at 1600 volts / 10 ms / 3 pulses ...
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bioRxiv - Neuroscience 2023Quote: Measurement of Aβ1-40 levels was performed using an Amyloid beta 40 Human ELISA Kit (Invitrogen, KHB3482). For further information please see Supplementary Material.
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bioRxiv - Immunology 2023Quote: ... A goat anti-Human IgG secondary antibody labelled with Alexa Fluor 488 was added (Thermo Fisher Scientific). The plates were incubated for 30 min at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Blots were probed with rabbit monoclonal anti-human CD38 antibody (Clone RM388, Thermo Scientific, cat# MA5-36061) after blocking with 5% BSA ...