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Citations for Thermo Fisher :
5901 - 5950 of 10000+ citations for Anti Epidermis type lipoxygenase 12 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Samples were boiled for 10 min at 70°C to and loaded on a 1.0 mm NuPage precast 4-12% (v/v) Bis-Tris gel (Invitrogen). SDS-PAGE and semi-wet transfer to a methanol-pre-activated polyvinylidene difluoride (PVDF ...
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bioRxiv - Cell Biology 2024Quote: ... human retinal pigment epithelial cells (RPE) cells were maintained in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F12, Gibco) containing L-Glutamine and supplemented with 10% (v/v ...
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bioRxiv - Developmental Biology 2024Quote: ... Transverse sections of 20 µm thickness were cut and collected on polarized microscope slides (Fisher Scientific 12-550-15). Sections were rehydrated with PBS and blocked for 1 hr at room temperature using a blocking solution (2% goat serum 2 mg/mL ...
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bioRxiv - Developmental Biology 2024Quote: FVB embryos were dissected from the uterine horn on ice in pre-chilled DMEM/F-12 with Glutamax (ThermoFisher), hereafter referred to as DMEM ...
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bioRxiv - Cell Biology 2024Quote: ... N2B27 basal media (500 ml) was prepared as follows: 482.5 ml DMEM/F-12 (Gibco, cat. no. 21331-020), 2.5 ml N2 supplement (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... Successful amplification of the wild-type p53 cDNA was confirmed by inserting the amplified candidate sequence into the TOPO® vector (TOPO® TA Cloning® Kit; Invitrogen™/Life Technologies™ ...
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bioRxiv - Immunology 2021Quote: HEK293T and RAW264.7 cells were obtained from the China Center for Type Culture Collection (Wuhan, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM; Invitrogen-Thermo Fisher; Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2021Quote: ... Salivary glands and midguts from each time point were pooled together according to tissue type and stored in RNAlater (Life Technologies, Carlsbad NM) at -80°C until used (Bullard et al. ...
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bioRxiv - Developmental Biology 2020Quote: ... The seminiferous tubules were digested in 5 mL of DPBS solution I consisting of 1 mg/mL collagenase type IV (17104019, Gibco, Grand Island, NY) and 20 U/mL DNase I (2212 ...
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bioRxiv - Neuroscience 2021Quote: ... Antibodies specific for cell type specific markers: GFAP (Millipore, Clone GA5, Cat#mab3402, Lot#2580632, 1:750 and Invitrogen, Lot#TF268267, 1:500), NeuN (Millipore ...
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bioRxiv - Molecular Biology 2021Quote: ... for 60 min followed by another digestion using 1000 U/ml collagenase type 2 and 11 U/ml dispase (Gibco, cat# 17105-041) for 30 min ...
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bioRxiv - Genomics 2020Quote: Optimal establishment of primary human hepatocytes in tissue culture required a substratum of type 1 collagen (24-well plates; ThermoFisher Scientific #A11428-02) and initial plating at 200,000 cells/well in complete Williams’ E medium containing supraphysiological concentrations of insulin ...
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bioRxiv - Cancer Biology 2020Quote: ... but with substitution of the Exonuclease I buffer pre-permeabilization treatment with a 20 min incubation at 37 °C in 14U of collagenase type I (Life Technologies, Paisley, UK).[18] The Exonuclease I buffer was diluted in 1x HBSS buffer (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: Wild-type and FAP-α overexpressing cells were seeded in an 8-well Lab-Tek II chamber slide (Nalge Nunc, Rochester, NY, USA) at a density of 10,000 cells/well overnight ...
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bioRxiv - Developmental Biology 2021Quote: Total RNA was extracted from 2×106 cells (3 biological replicates for wild-type and CNOT3-DM cells) using Trizol reagent (Thermo Fisher Scientific, USA) following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... biocompatibility of the different gel formulations or tissue construct types was assessed using a standard LIVE/DEAD cell viability/cytotoxicity kit (Thermo Fisher, Waltham, MA) according to the manufacturer’s recommendation ...
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bioRxiv - Bioengineering 2020Quote: ... all types of porous silk scaffolds described above were placed in hepatocyte culture medium containing 10% fetal bovine serum (FBS, Thermo Fisher, Waltham, MA) overnight to promote protein binding to the silk surface before cell seeding ...
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bioRxiv - Bioengineering 2021Quote: ... Excessive gels around the tissue sample were trimmed away using a razor blade. Tissues that were designated for the collagenase treatment (Supp. Fig. 4C) were submerged in collagenase type II (Thermo Fisher Scientific, #17101015) at 1500 U/ml in Hank’s balanced salt solution (HBSS ...
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bioRxiv - Plant Biology 2020Quote: ... Three wild-type or five rdr2 mutant embryos were pooled before DNA extraction with the GeneJET Plant Genomic DNA Purification Kit (Thermo Fisher Scientific, K0791). Embryos from rdr2/RDR2 heterozygous mothers were individually collected ...
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bioRxiv - Plant Biology 2021Quote: ... Change in the PM potential was monitored in wild-type pollen grains with a the use of DiOC2(3) (Thermo Fisher Scientific, USA) that accumulates in hyperpolarized membranes and DiBAC(4)3 (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... 9 x 105 cells of each indicated SARS-CoV-2-non-infectable cell type were stained with CellTrace™ Violet (Thermo Fisher # C34557), following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... brains from wild-type C57Bl6/J mice (age P0-3) were removed immediately after euthanization and digested with Trypsin (ThermoFisher, Cat. No. 25300054) for 15 min at 37°C ...
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bioRxiv - Cell Biology 2022Quote: The RAW 264.7 murine macrophage cell line (American Type Collection Culture [ATCC]; Manasas, VA) was maintained in 100-mm diameter tissue culture-treated polystyrene dishes (Fisher Scientific, Hampton, NH) at 37°C in a humidified atmosphere of 95% air and 5% CO2 ...
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bioRxiv - Developmental Biology 2022Quote: ~2-3 mm long uterine sections collected from the midpoint of the uterine horn encompassing all tissue types (myometrium and endometrium) were placed into TRIzol Reagent (Life Technologies, Cat. #15596) and stored at −80 degrees until extraction ...
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bioRxiv - Cell Biology 2022Quote: ... cell line were purchased from the American Type Culture Collection (ATCC) and cultured in RPMI 1640 medium ( Cat# 11835030, Gibco, no phenol red) supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin (Cat# 15140122 ...
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bioRxiv - Molecular Biology 2020Quote: ... The copy ratio of mutant to wild type allele in each sample was determined using QuantStudio™ 3D AnalysisSuite™ software (Applied Biosystems). The DNA from the sequenced samples was tested along with DNA from uninfected wheat leaf ...
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bioRxiv - Immunology 2020Quote: ... were obtained from the American Type Culture Collection (ATCC, Manassas, VA) and cultured in Dulbecco’s Modified Eagle Medium (DMEM; ThermoFisher Scientific, Grand Island, NY) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2021Quote: Two μg of wild-type or mutant S was transfected into Vero or A549 cells using the Lipofectamine 3000 system (Invitrogen; ratios described above). Eighteen to twenty-four hours post transfection ...
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bioRxiv - Pathology 2022Quote: ... Bone samples were cut into 1–2 mm pieces and then digested in 0.2% type II collagenase (17101015, Thermo Fisher Scientific, MA, USA) for 2 h ...
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bioRxiv - Biochemistry 2022Quote: ... Constructs for wild-type receptors were cloned into MIG by Gibson assembly as described above with the BstBI (Fisher Scientific 50-812-170) and NotI (Fisher Scientific 50-811-2040 ...
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bioRxiv - Genetics 2022Quote: Human embryonic kidney cell line (HEK 293T) was purchased from the American Type Culture Collection and cultured in DMEM medium (GIBCO, Cat. NO. 12430) supplemented with 10% FBS (GIBCO ...
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bioRxiv - Systems Biology 2020Quote: The HNSCC cancer cell line FaDu was purchased from the American Type Cell Collection (ATCC) and cultured in Minimum Essential Medium (MEM, Gibco™/Life Technologies) with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Cancer Biology 2020Quote: ... The bones were broken into fragments and incubated for 1 hour at 37 °C in phosphate-buffered saline (PBS) supplemented with 1 mg/ml collagenase type I (Thermo Fisher, Waltham, US). The supernatants were filtered through 100 μm pores of a cell strainer (Greiner Bio-One ...
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bioRxiv - Immunology 2020Quote: ... and digested twice for 30 minutes each at 37°C with 1.0 mg/ml type IV collagenase (Gibco, 9001121, Grand Island, NY, USA) and 10U/ml type I DNase (Sigma ...
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bioRxiv - Cancer Biology 2021Quote: Human AML cell lines U937 and HL60 were purchased from the American Type culture collection (ATCC; Manassas, USA) and maintained in minimum essential medium-α medium (Gibco, Carlsbad, USA). Murine AML cell line C1498 was obtained from ATCC and cultured in Dulbecco’s modified eagle’s medium (Gibco ...
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bioRxiv - Immunology 2020Quote: Wild-type Phl p 6 and its mutants were expressed from pET17b constructs in Escherichia coli strain BL21 Star (DE3; Invitrogen/Thermo Fisher Scientific), as described in the supplement.
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bioRxiv - Cell Biology 2021Quote: ... The filtrate was enzymatically digested for 1 h with 500 μg ml-1 Collagenase type II (Cat no.: 17101-015, Gibco, Grand Island, NY)) ...
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bioRxiv - Neuroscience 2020Quote: Nuclear fractionation from hippocampus of 30-week old wild-type and R6/1 mice was performed using a Subcellular Protein Fractionation Kit for Tissues (Thermo Fisher Scientific #87790) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... hindlimb tibialis anterior (TA) muscles from E18.5 mouse embryos were explanted and digested in 0.2% collagenase type I (Gibco-Life Technologies, catalog no. 17100-017) in DMEM for 60 min at 37°C ...
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bioRxiv - Immunology 2020Quote: ... isolated SI CD8α+ IET (EpCAM-CD4-CD19-TER119- CD8α+) from wild type mice were labeled with CellTrace™ Violet (CTV, Thermo Fisher Scientific), and then labeled cells were incubated in the presence of recombinant IL-15/15Rα (50ng/mL ...
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bioRxiv - Cancer Biology 2021Quote: ... were obtained from the American Type Culture Collection (ATCC, Manassas, VA) and were maintained in RPMI 1640 medium with ATCC modification (Thermo Fisher Scientific-Gibco, CA, USA), supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2023Quote: ... Cortical tissue was cut into 1 mm3 fragments and digested with 1 mg/ml collagenase type II at 37° C in DMEM (Invitrogen, Grand Island, NY) for 30 minutes ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: HepG2 (human hepatocellular carcinoma) cells were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) and cultured in DMEM medium (Gibco™, GlutaMAX™) containing 4.5 g/LD-glucose and pyruvate ...
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bioRxiv - Developmental Biology 2023Quote: ... the dome-shaped colonies were first detached from feeders by exposure to 1 mg/ml Collagenase type IV (Thermo Fisher Scientific, 17104-019). Based on the different detaching speed of colonies ...
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bioRxiv - Cancer Biology 2023Quote: ... were purchased from the American Type Culture Collection (ATCC) (Manassas, VA, USA) and cultured in Roswell Park Memorial Institute Medium 1640 (Gibco, Waltham, MA, USA) supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Microbiology 2023Quote: The wild-type ISG20 full coding sequence (amino acids 1–181) was cloned into the pPROEX HTa vector (Thermo Fisher Scientific, cat. 10711018) by standard molecular biology techniques ...
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bioRxiv - Synthetic Biology 2023Quote: ... Wild-type I-OnuI was previously cloned between the NcoI and NotI cut sites on the pProEX-HT-a plasmid (Invitrogen and Life Technologies) and pEndo backbone49 ...
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bioRxiv - Physiology 2023Quote: ... hTERT-HM cells were suspended in a collagen gel matrix (Cellmatrix collagen type I-A, Fisher Scientific, prepared with MEM, NaOH and HEPES) then plated in 12-well dishes and grown in hTERT-HM medium ...
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bioRxiv - Immunology 2023Quote: Human colon cancer cell line RKO obtained from the American Type Culture Collection (Cat.No. CRL-2577) were maintained in RPMI 1640 GlutaMAX™ culture medium (Gibco, Cat.No. 61870-010) supplemented with 10% (v/v ...
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bioRxiv - Cell Biology 2024Quote: ... hiPSC colonies were detached in clusters using a 13 min incubation with collagenase type IV (250 IU/mL final concentration) (Thermo Fisher Scientific; 17104019) and transferred to 6-well low adhesion plates in ESC media supplemented with 10 µM ROCK Inhibitor (Sigma ...