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Citations for Thermo Fisher :
5851 - 5900 of 10000+ citations for Anti Epidermis type lipoxygenase 12 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... 0.2% bromophenol blue) SDS-PAGE analyses were performed with 4%–12% NuPAGE bis-tris gels (Thermo Fisher Scientific, NP0336) and immunoblotted on nitrocellulose membranes (BioTrace Pall Laboratory ...
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bioRxiv - Microbiology 2024Quote: ... and HS-deficient mutant CHO-pgsD-677 [ATCC CRL-2244] cells were maintained in Ham’s F-12 medium supplemented with 10% heat-inactivated fetal bovine serum (FBS, Gibco). Human lymphoblast K562 cells expressing VLDLR ...
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bioRxiv - Molecular Biology 2024Quote: 30 μL of protein extract were subjected to SDS-PAGE on a 10-well NuPAGE 4–12% gradient (Invitrogen) for 6 min at 200 V with MES buffer ...
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bioRxiv - Neuroscience 2024Quote: ... including β-mercaptoethanol and separated with SDS-PAGE using NuPAGE 4–12 % Bis-Tris Midi Protein Gels (#WG1402BOX, Invitrogen). Proteins were subsequently transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Neuroscience 2024Quote: ... 20µg of protein per sample was loaded onto NuPAGE 4- 12% Bis-Tris gels (Thermo Fisher Scientific, catalogue #NP0336BOX). Electrophoresis was performed in NuPAGE MES SDS running buffer (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: 10 µg of protein from the bead eluent were loaded on 4 to 12% gradient Bis-Tris gels (ThermoFisher) and run until loading dye reached bottom of gel ...
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bioRxiv - Neuroscience 2024Quote: ... they were cut at a thickness of 6 micrometers and mounted on coated slides (Fisher scientific #12-550-109). All other procedures followed the manufacturer’s instructions (RNAscope® 2.5 HD Duplex Reagent Kit ...
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bioRxiv - Neuroscience 2024Quote: ... The larval brains (N=12) were then immediately placed in RNase-free 1.5ml tubes (containing 100µl of TRIzol lysis reagent, Invitrogen, USA) on ice ...
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bioRxiv - Microbiology 2024Quote: ... low-adhesion plates in 100 µL of N2B27 differentiation medium (1:1 [v/v] of DMEM/F-12 [GIBCO] and neurobasal medium [GIBCO] supplemented to contain 10% Knockout serum replacement [Invitrogen] ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were analysed by 12% SDS-polyacrylamide gel electrophoresis (PAGE) prior to Coomassie R-250 or silver staining (Thermofisher).
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bioRxiv - Plant Biology 2024Quote: ... NbISIC1 and its mutant were induced with 0.1 mM IPTG for 16 h at 12℃ and purified using Ni-NTA agarose resin (ThermoFisher). Proteins were concentrated to 50 μM in pull-down buffer containing 20 mM HEPES (pH 7.5) ...
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bioRxiv - Biochemistry 2024Quote: ... Cleavage of recombinant protein was verified by running sample on a Bis-Tris 4-12% gel (Thermo Fisher, NP0321), resolved by electrophoresis in 1x MOPS SDS running buffer (Thermo Fisher ...
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bioRxiv - Neuroscience 2024Quote: ... The sections were washed with 1× PBS and mounted onto super-frost slides (Thermo Fisher Scientific, 12-550-15). The z-stack images of dendritic spine segments were acquired using a confocal microscope (Zeiss LSM 700 microscope ...
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bioRxiv - Microbiology 2024Quote: ... mucus lysates (10 µg of proteins) were loaded onto a Bolt 4–12% Bis-Tris gradient gel (#NW04120BOX, Invitrogen). Proteins were separated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Systems Biology 2024Quote: ... ∼4×10^4 hESCs were plated onto a single well of a Vitronectin-coated 12-well dish (Gibco, A14700) in Nutristem hPSC XF medium (Biological Industries ...
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bioRxiv - Molecular Biology 2024Quote: Samples from in cellulo or in organello labelling were loaded onto a 12% NuPAGE Bis-Tris gel (ThermoFisher Scientific) and electrophoresed at 150V in an XCell SureLock system (ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... 50 μg of sample was then loaded onto an SDS PAGE pre-cast 4-12% Bis Tris gel (ThermoFisher) and separated with MOPS buffer (ThermoFisher) ...
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Entorhinal cortex vulnerability to human APP expression promotes hyperexcitability and tau pathologybioRxiv - Neuroscience 2024Quote: ... 2/3rd of the eluted protein samples were resolved on a 4–12% Bris-Tris gel (Invitrogen: cat# NW04125box) and subjected to Silverstein (Pierce™ Silver Stain Kit ...
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bioRxiv - Neuroscience 2024Quote: ... cell samples were lysated and loaded in precast polyacrylamide gels (NuPage 4-12%, Bis-Tris Mini protein gel, Invitrogen) immersed in MOPS SDS running buffer (cat ...
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bioRxiv - Physiology 2024Quote: ... NRCM mitochondrial pellets were digitonin solubilized and 10ug protein was run on 3-12% Bis-Tris NativePAGE gels (ThermoFisher) using “dark blue” running buffer and changed to “light blue” running buffer about 1/3 of the way through ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... a 12 μL reaction mix was created: 6 μL SYBR Green PCR Master Mix (Thermo Fisher Scientific, Cat#: 4309155), 2 μL diluted primer pairs ...
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bioRxiv - Neuroscience 2024Quote: ... and 10μg of total protein was loaded onto 4–12% NuPAGE Bis-tris gels in MES buffer (Invitrogen; Cat:NP0323). After electrophoresis ...
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bioRxiv - Molecular Biology 2024Quote: ... The samples were separated on a NativePAGE 3-12% Bis Tris protein gel (Thermo Fisher Scientific, cat. no. BN1001BOX) in a 25mM Tris ...
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bioRxiv - Molecular Biology 2024Quote: ... The correct size of purified Cas9 proteins was confirmed on a NuPAGE 12% Bis-Tris Gel (Thermo Fisher Scientific) and concentration was determined using the Bradford assay [42].
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bioRxiv - Pharmacology and Toxicology 2024Quote: HESCs were passaged under normoxic conditions (20% O2) while cultured in Phenol red-free Dulbecco’s modified Eagle medium (DMEM)/F-12 (Gibco) supplemented with 5% charcoal dextran-stripped FBS (Atlanta Biologicals) ...
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bioRxiv - Microbiology 2024Quote: Total cell lysates prepared in RIPA or Laemmli buffer were loaded on 4-12% gradient acyrylamide gels (Invitrogen, NP0323). Immunoblot PVDF membranes (GE Healthcare Life science ...
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bioRxiv - Neuroscience 2024Quote: ... or RNA and protein extraction (18 mm cover glasses [631-0153; VWR] in 12 well plates [142475; Thermo Fisher]). For protein extraction and FISH ...
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bioRxiv - Physiology 2024Quote: ... The samples and protein ladder were loaded onto a native PAGE 3-12% Bis-Tris Gel (Thermo Fisher Scientific). Dark blue cathode buffer (0.02% Coomassie Brilliant Blue dissolved in Native Page running buffer ...
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bioRxiv - Genomics 2024Quote: ... Equal amounts of total protein were run on a 4 to 12% polyacrylamide Bis-Tris gel (ThermoFisher Scientific, NW04125BOX) then transferred to a nitrocellulose membrane ...
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bioRxiv - Immunology 2024Quote: ... and primary lung (RaLu1.4) [34] were maintained in DMEM/F-12 supplemented with 1X MEM non-essential amino acids (NEAA) (Gibco), 10% FBS ...
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bioRxiv - Immunology 2024Quote: ... Equal amounts of proteins were loaded in wells of a 4-12% NuPAGE Bis-Tris Gel (cat #NP0322BOX, Invitrogen) and blotted to a PVDF membrane (catalog #IPFL00010 ...
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bioRxiv - Genetics 2024Quote: ... The retinas were cryosectioned at 15 µm thickness onto Superfrost plus slides (Fisher Scientific Cat no. 12-550-15) using a cryostat (Leica Biosystems) ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were cultured at a density of 1 × 106 cells/ml in 12 well plates with AIMV medium (Invitrogen) containing 10% FCS ...
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bioRxiv - Genomics 2024Quote: Tissue pools were dissociated to single cells via incubation in TrypLE Express Enzyme (12-604-013, Thermo Fisher Scientific) for 15 min at 37°C on a shaker ...
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bioRxiv - Neuroscience 2024Quote: ... at 99°C for 10 min and proteins were separated on NuPAGE 4–12% Bis-Tris Gels (ThermoFisher Scientific). They were then transferred onto a PVDF membrane using the iBlot system (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... organoids were transferred to a tissue culture plate and cultured in suspension in N2B27 medium [DMEM/F-12 (Gibco), neurobasal medium (Gibco ...
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bioRxiv - Genomics 2024Quote: ... Human lung tissue was filled with 3 % of low gelling temperature agarose in DMEM/F-12 (Thermo Scientific, USA) with phenol red supplemented with 0.1 % FCS ...
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bioRxiv - Biochemistry 2024Quote: ... The purity of protein preparations was assessed using SDS-PAGE with NuPAGE 4-12% Bis-Tris protein gels (Invitrogen) followed by OneStep Blue (Biotium ...
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bioRxiv - Biophysics 2024Quote: ... 5 μl of protein lysate was separated by gel electrophoresis on a NuPAGE 4-12% Bis-Tris Gel (ThermoFisher) and transferred by semi-dry transfer in NuPAGE transfer buffer (ThermoFisher ...
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bioRxiv - Cell Biology 2024Quote: ... then fixation/permeabilisation and staining for nuclear protein FOXP3 for regulatory T cells (Life Technologies, Cat: 12-4776-42). All cells were measured using the Cytoflex LX flow cytometer and analysed with FCS Express 7 ...
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bioRxiv - Bioengineering 2024Quote: ... cell culture was maintained at 37 °C and 5% CO2 in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (Gibco™ DMEM/F- 12 ...
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bioRxiv - Cancer Biology 2024Quote: ... CIOV1, 2, 3 cell lines (Brenton Lab, University of Cambridge, UK) were cultured in DMEM/F-12 medium (Gibco) with 10% FBS and 1% penicillin/streptomycin ...
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bioRxiv - Cell Biology 2024Quote: ... was boiled for 5 minutes at 95°C and separated on a 4-12% Bis-Tris gel (NP0321BOX, Invitrogen) in MES running buffer (NP0002 ...
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bioRxiv - Cancer Biology 2024Quote: ... Equal amounts of total protein (30 μg) were separated by NuPAGE Bis-Tris (4–12%) gels (Thermo Fisher Scientific) and transferred to a nitrocellulose membrane (Amersham ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were then mounted onto plain 25 × 75 × 1 mm microscope slides (Catalog No: 12-544-4; Fisher Scientific). Time-lapse images were acquired with an inverted Nikon Eclipse Ti2-E microscope with a Yokogawa confocal scanner unit (CSU-W1) ...
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bioRxiv - Bioengineering 2024Quote: ... The denatured protein samples were subsequently separated in a Bolt 4–12% Bis-Tris pre-cast gel (Thermo Fisher) and transferred onto a PVDF membrane using an iBlot 2 gel transfer system (Thermo Fisher) ...
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bioRxiv - Bioengineering 2024Quote: ... 10 µL of the sample was loaded onto a pre-cast NuPAGE Novex 4-12% Bis-Tris 1.0 mm mini-gel (Invitrogen). For each gel run ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 μl of cell solution was lysed on slides (Superfrost Plus microscope slides, Fisher Scientific, cat# 12-550- 15) with 200 mM Tris–HCl ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 x 103 cells were lysed on slides directly (Superfrost Plus microscope slides, Fisher Scientific, cat# 12- 550-15) with 7.5 μL of lysis buffer (200 mM Tris–HCl ...
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bioRxiv - Cancer Biology 2024Quote: ... then denatured at 95°C for 5 minutes and loaded on 4-12% NuPAGE™ Bis-Tris gels (Invitrogen). Samples were transferred to a nitrocellulose membrane using a semi-wet transfer ...