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Citations for Thermo Fisher :
5801 - 5850 of 10000+ citations for Anti Epidermis type lipoxygenase 12 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... brains were sectioned at 20 μm thickness and mounted onto SuperFrost Plus Slides (Fisher Scientific, Cat# 12-550-15). After drying in the air ...
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bioRxiv - Microbiology 2023Quote: HCT-8 cells were plated at 4.5 x 105 cells per 12-mm-diameter glass coverslip (Thermo Fisher Scientific) in 24-well tissue culture plates and incubated until confluency (∼24 h) ...
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bioRxiv - Molecular Biology 2023Quote: ... Equivalent protein quantities for all genotypes were loaded onto a Nupage 4-12% Bis-Tris Protein Gels (ThermoFisher Scientific) for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... containing 3 mL of home-made Expansion Medium consisting of a 1:1 mixture of DMEM/F-12 (Gibco) and Neurobasal Medium (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... Protein purity and size were checked trough SDS-Page on Novex WedgeWell 12% Tris-Glycine Gel (Invitrogen, Waltham, US). The protein ladder used was Colour Protein Standard Broad Range (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... 40 μg of protein were loaded and run on pre-casted 4-12% NuPAGE Bis-Tris gels (Invitrogen #NP0335) for 90 minutes at 120V ...
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bioRxiv - Immunology 2023Quote: ... PANC-1 and MiaPaCa-2 were cultured and maintained in growth medium [DMEMF/12 with GlutaMAX™ Supplement (Gibco), + 10% v/v Heat Inactivated Fetal Bovine Serum (FBS ...
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bioRxiv - Bioengineering 2023Quote: ... The solution was transferred to dialysis membranes (12 -14 kDa molecular weight cut off; Fisher Scientific 21-152-8) and dialyzed against deionized water for 7 days and subsequently lyophilized (LABCONCO).
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bioRxiv - Synthetic Biology 2023Quote: ... Images were acquired with a 120 kV FEI Tecnai 12 electron microscope equipped with a Ceta camera (Thermo Fisher).
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bioRxiv - Neuroscience 2023Quote: ... followed by seeding of 2-3×106 cells onto T25 flasks (Thermo-Fisher Scientific; Cat. No. 12-556-009) coated with Matrigel ...
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bioRxiv - Biochemistry 2023Quote: ... incubated for 30 min at 4 °C and analyzed on 3-12% gradient Bis-Tris native gels (Life Technologies) at 4 °C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cells between passages 12-24 were cultivated in DMEM/Ham’s F-12 medium (1:1 Mix) supplemented with L-glutamine and 15 mM HEPES (Gibco™ ...
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bioRxiv - Physiology 2023Quote: ... The samples were run under reducing conditions with 4-12% Bolt™ Bis-Tris Plus pre-cast gels (Invitrogen) and proteins were transferred to a nitrocellulose membrane ...
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bioRxiv - Genomics 2023Quote: ... 0.5x106 mESCs were plated to gelatin-coated T25 flasks in N2B27 media (50:50 DMEM/F-12:Neurobasal (Gibco) supplemented with 1X N2 and 1X B27 (ThermoFisher) ...
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bioRxiv - Microbiology 2023Quote: ... The cultures were incubated for 2 h at 37°C with 5% CO2 and subsequently washed three times with Advanced Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F12 (Gibco)) ...
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bioRxiv - Immunology 2023Quote: Proteins in VLP containing samples were separated by SDS-PAGE using 4-12% Bis-Tris Nu-PAGE gels (Invitrogen) and electro-transferred to a PVDF membrane using the Trans-Blot Turbo Transfer Pack (BioRad) ...
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bioRxiv - Microbiology 2023Quote: ... 2 ug of EV protein content were separated on a 4-12% Bis-Tris gel (Thermo Scientific, Rockford, IL), then blotted onto a PVDF membrane ...
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bioRxiv - Cancer Biology 2023Quote: ... The supernatant was then electrophoresed on a 3-12% Native PAGE Novex Bis-Tris gel (Invitrogen, Inc. Waltham, MA) at 80 V for 4 hours at 4°C in the supplier’s buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... TrypLE reaction was quenched via dilution with cold Splitting Media (Advanced DMEM/F-12 [Gibco], 10 mM HEPES [Invitrogen] ...
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bioRxiv - Cell Biology 2023Quote: ... lysates were separated by SDS-PAGE gel electrophoresis on 1 mM NuPAGE 4 – 12% bis-tris gels (Thermo Fisher) using MOPS running buffer and transferred to nitrocellulose membranes using Tris-Glycine-methanol transfer buffer (30.3 g Tris Base ...
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bioRxiv - Cell Biology 2023Quote: ... 8 µg of protein was separated on a NuPAGE 4–12% Bis-Tris gel (Life technologies, Carlsbad, CA, USA). Proteins were electroblotted to 0.45 µm PVDF membranes (Imobilon-P ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then transferred to 10cm dishes coated with Matrigel (Corning Cat# 354234 diluted in DMEM/F-12, Gibco). 48 hours later cells were fed with a 1:1 mixture of complete DMEM and E8 media (TeSRTM-E8TM Stem Cell Technologies) ...
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bioRxiv - Microbiology 2023Quote: ... the specimens were cut at 20-μm thickness and mounted on positively charged slides (Fisher Scientific #12-550-15). Nissl staining was performed ...
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bioRxiv - Genomics 2023Quote: ... 25 mL of wash solution (DMEM/F-12 supplemented with 0.03% GIBCO BSA Fraction V, pre-warmed at 37C) was added to each plate to inactivate the trypsin ...
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bioRxiv - Cell Biology 2023Quote: ... Induction into neural crest iPericytes was achieved by culturing in DMEM/F-12 plus GlutaMAX (Thermofisher Scientific, cat.#10565018) supplemented with 0.5% (v/v ...
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bioRxiv - Molecular Biology 2023Quote: ... SDS-PAGE gels were loaded with 5-15 μL of lysate per lane (NuPAGE 4-12% Bis-Tris; Invitrogen). Lysates were diluted with additional 1x NuPAGE LDS buffer if necessary ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were separated using SDS polyacrylamide gel electrophoresis (Bolt™ 4-12% Bis-Tris Plus Gel, Thermo Fisher Scientific) and further transferred onto a PVDF membrane (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... GCT cell lines were routinely maintained at 37°C with 5% CO2 in complete medium consisting of Dulbecco’s modified Eagle medium: nutrient mixture F-12 (DMEM/F12) (Gibco) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Biophysics 2024Quote: We purchased premium plain glass microscope slides (75 mm × 25 mm, catalog number: 12-544-1) from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Cell Biology 2024Quote: ... At day 12 the obtained neuroepithelial sheet was harvested and dissociated using the enzyme Dispase (Life Technologies, cat# 17105) and plated on laminin-coated plates ...
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bioRxiv - Immunology 2024Quote: Protein lysates were separated using 4-12% Bis-Tris SDS-polyacrylamide gel electrophoresis (SDS-PAGE) (Thermo Fisher Scientific, #NP0321) for 1-2 h at 150V ...
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bioRxiv - Neuroscience 2024Quote: ... The cells were then triturated and plated on a 12 mm Nunc glass base dish (Cat# 150680; Thermo Scientific) coated with poly-D-lysine and mouse laminin I ...
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Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: ... Parental A549 cells (BEI Resources #NR-52268) were cultured in Ham’s F-12 K (Kaighn’s) medium (Fisher Scientific #21127030) with 10% FBS ...
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bioRxiv - Neuroscience 2024Quote: ... NSCs were maintained and expanded in NS expansion medium composed as follows: DMEM/Ham’s F-12 media with l-glutamine (Gibco), Glucose solution 29mM (Signa-Merck) ...
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bioRxiv - Microbiology 2024Quote: ... The LOS extracts were then run on NuPAGE Bis-Tris gradient (4-12%) SDS-PAGE gels (Invitrogen, Waltham, USA) with NuPAGE MES running buffer (Invitrogen ...
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bioRxiv - Synthetic Biology 2024Quote: ... The samples were first separated by 12% SDS-PAGE gel and then transferred to a nitrocellulose membrane (Fisher Scientific). The membranes were incubated with the Anti-FLAG epitope (DYKDDDDK ...
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bioRxiv - Immunology 2024Quote: ... GPI-RBD-hGM-CSF recombinant fusion protein and GPI-hIL-12 were affinity purified from CHO-S cells (Invitrogen) stably expressing the proteins using anti-human GM-CSF (clone BVD2-21C11 ...
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bioRxiv - Immunology 2024Quote: ... heated to 70°C for 5 min and ran on a 4-12% Nupage precast gel (Thermo Fisher Scientific). Protein were then detected using InstantBlue™ Coomassie Stain (Abcam ...
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bioRxiv - Physiology 2024Quote: ... boiled at 95°C for 5min and electrophoresed on BoltTM 4-12% Bis-Tris Plus gel (Thermo Fisher, NW04122BOX) at 200V for 20min ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The resulting PM suspensions were then diluted with high-glucose Dulbecco’s Modified Eagle Medium /Nutrient Mixture F-12 (CAS 31331-028, DMEM/F12, Gibco) to reach a PM concentration of 1 mg mL−1 and sonicated for an additional 5 min ...
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bioRxiv - Cell Biology 2024Quote: ... Lysates were clarified by centrifugation and were resolved with NuPAGE 4-12% Bis-Tris protein gel (Thermo Fisher Scientific) prior to transfer onto nitrocellulose membranes ...
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bioRxiv - Molecular Biology 2024Quote: ... Transgenic CHO cells were maintained in an F-12 Nutrient Mixture with GlutamaxTM-I (Thermo Fisher Scientific, MA, USA), 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2024Quote: Mouse mesothelioma cell lines with stable Cas9 were previously generated in our laboratory and cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F12+Glutamax; Gibco), supplemented with 4 μg/ml Hydrocortisone (Sigma) ...
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bioRxiv - Neuroscience 2024Quote: ... the expansion medium was replaced with the following differentiation medium: Neurobasal plus DMEM/Ham’s F-12 media with l-glutamine 1:1 (Gibco), N2 supplement 1x (Gibco) ...
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bioRxiv - Cell Biology 2024Quote: ... Human precision cut lung slices (PCLS) were purchased from IIVS and were grown in DMEM/F-12 media (Gibco) supplemented with antibiotics and antimycotic reagents.
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bioRxiv - Developmental Biology 2024Quote: Retinas were cultured on 13 mm/0.2 micron floating filters (Cytiva, 10417001) on DMEM/F-12 media (Gibco, 11320082) with 10% Fetal Bovine Serum (Thermofisher ...
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bioRxiv - Molecular Biology 2024Quote: ... Time points were resolved by SDS-PAGE on NuPAGE™ Novex™ 4-12% Bis-Tris Protein Gels (Invitrogen) in MOPS running buffer ...
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bioRxiv - Neuroscience 2024Quote: ... 10-20 µg protein were loaded onto 4-12% Bis-Tris polyacrylamide gel (Thermo Fisher Scientific; Cat No. NP0336BOX) Nitrocellulose (BioRad ...
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bioRxiv - Synthetic Biology 2024Quote: ... and insoluble protein using SDS-PAGE (NuPAGE® 4–12% bis-tris gel (Thermo Fisher Scientific, Waltham, MA, USA)) to separate proteins in the mixture by molecular weight ...
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bioRxiv - Neuroscience 2024Quote: ... Neuronal differentiation medium contained Dulbecco’s Modified Eagle Medium: Nutrient Mixture F-12 with GlutaMAX supplement (Thermo Fisher Scientific # 10565018), 1X N-2 Supplement (Thermo Fisher Scientific # 17502048) ...