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Citations for Thermo Fisher :
5751 - 5800 of 10000+ citations for Anti Epidermis type lipoxygenase 12 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... 12 hrs) by UV-VIS spectrophotometer at an absorbance of 233 nm (Nanodrop, Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Microbiology 2023Quote: ... A synthetic plasmid control based on bp 740–891 of the F14.1158H (OQ110629.1, (12)) was ordered from GeneArt (ThermoFisher Scientific). Analytic sensitivity of the PCR was determined with a plasmid dilution series of 0 ...
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bioRxiv - Bioengineering 2023Quote: ... MPCs were cultured at 37°C and 5% CO2 in Hams F-12 Nutrient Mixture (Thermo Fisher, Waltham, MA) supplemented with 20% FBS (Biotechne ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then plated for apoptosis at 200,000 cells in 12 well plates in DMEM/F12 media (ThermoFisher, 11330032) with an additional 4 mM Glutamax (ThermoFisher ...
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bioRxiv - Neuroscience 2023Quote: ... we collected nuclei for processing with 10X multiome as follows: Cells were dissociated with TrypLE (Gibco 12-605-010) for 15 min at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... CCL-185) and A549 TP53ko cells132 (a gift from Dr. William Hahn) were cultured in DMEM/F-12 (Gibco) supplemented with 10% FBS (Sigma ...
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bioRxiv - Bioengineering 2023Quote: ... were cultured in serum free medium (1X KnockOut D-MEM/F-12 with 2% StemPro Neural Supplement (ThermoFisher A1050801), 20 ng/mL bFGF ...
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bioRxiv - Bioengineering 2023Quote: ... Neuronal differentiation medium contained Dulbecco’s Modified Eagle Medium: Nutrient Mixture F-12 with GlutaMAX supplement (Thermo Fisher Scientific # 10565018), 1X N-2 Supplement (Thermo Fisher Scientific # 17502048) ...
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bioRxiv - Microbiology 2023Quote: Basal media samples collected during the infection process were separated on BoltTM 4-12% Bis-Tris gradient gels (ThermoFisher) using 20 μg protein/lane and BoltTM Loading Dye (ThermoFisher) ...
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bioRxiv - Molecular Biology 2023Quote: ... Fibroblasts were cultured at 37° C at 5% CO2 in Dulbecco’s modified Eagle’s medium with nutrient mixture F-12 (Gibco-11330) and supplemented with 15% fetal bovine serum ...
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Functional characterization of ATP13A2 variants associated with distinct neurodegenerative disordersbioRxiv - Molecular Biology 2023Quote: ... 10 μg of protein was loaded on a NuPage 4-12% Bis-Tris gel (Thermo Fisher Scientific, Bio-Rad) and separated during an electrophoresis run at 150 V in MES running buffer (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2023Quote: ... and cellular debris spun down at 12,000 g for 5 minutes before being subjected to electrophoresis on Bolt 4- 12% Bis-Tris gels from Invitrogen/Thermo Fisher (Cat ...
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bioRxiv - Microbiology 2023Quote: ... separating proteins by electrophoresis with Chameleon Pre-Stained Protein Ladder (Li-Cor) on 4–12% Bis-Tris Gels (Invitrogen) at 150 V for 90 min ...
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bioRxiv - Microbiology 2023Quote: ... and loaded 10 µl (100 ng) of the sample on NuPAGE 4–12% Bis-Tris Protein Gel (Thermo Fisher), separating proteins by electrophoresis with Chameleon Pre-Stained Protein Ladder (Li-Cor ...
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bioRxiv - Molecular Biology 2023Quote: ... The other culture condition is the chemically defined serum-free 2i condition defined as follow: Neurobasal:DMEM/F-12 (50:50, Gibco) medium ...
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bioRxiv - Cancer Biology 2023Quote: ... Keratinocytes were cultured in Keratinocyte serum-free media (KSFM) containing: Advanced DMEM/F-12 media (Thermo Fisher, 12634-010), 1x B27 Supplement (Thermo Fisher ...
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bioRxiv - Cancer Biology 2023Quote: ... Comparable amounts of proteins were subjected to SDS-PAGE using NuPAGE 4% to 12% Bis–Tris gradient gels (Invitrogen) and were transferred to PVDF membranes ...
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Remodeling of the Ribosomal Quality Control and Protein Translation by a Viral Ubiquitin DeconjugasebioRxiv - Cell Biology 2023Quote: ... Equal amounts of lysates were fractionated in acrylamide Bis-Tris 4-12% gradient gel (Life Technologies Corporation, Carlsbad, USA). After transfer to PVDF membranes (Millipore Corporation ...
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bioRxiv - Cancer Biology 2023Quote: ... 15 µg of each sample was loaded on a NuPage 4-12% Bis-Tris gel (Thermo Fisher Scientific, USA) and run at 180 V for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were separated in an NuPAGE™ Novex™ 4-12% Bis-Tris protein gel (WG1402BOX, Thermo Fisher Scientific) in NuPAGE™ MOPS SDS running buffer (50 mM MOPS ...
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bioRxiv - Cell Biology 2022Quote: ... samples were transferred to a 12-well plate and digested with 7.5 U/ml Proteinase-K (Thermo Fisher, EO0491) in TAE buffer (containing 40 mM Tris ...
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bioRxiv - Genomics 2023Quote: ... at a density of 2.5 x 104 cells per cm2 in N2B27 medium defined as follows: 0.5x Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM-F12, Gibco, 11320033), 0.5x Neurobasal medium (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... 1 μg of total RNA was reverse transcribed using an oligo-(dT)12-18 primer and Superscript III (Invitrogen). Quantitative PCR gene expression analysis was conducted using a LightCycler 480 Real-Time PCR System (Roche ...
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bioRxiv - Developmental Biology 2023Quote: Live embryos were dissected as described above and transferred to culture media containing DMEM/F-12 (Gibco 10565-018), 10% fetal bovine serum ...
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bioRxiv - Immunology 2023Quote: ... human stool extracts and mouse colonic tissue lysates were resolved on Bolt 4-12% Bis-Tris Plus Gels (Invitrogen), transferred onto polyvinylidene difluoride membranes ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were separated by SDS-PAGE using a 4-12% NuPAGE Bis-Tris gel (Thermo Fisher Scientific Cat# NP0321BOX) in NuPAGE MOPS SDS running buffer (Thermo Fisher Scientific Cat# NP0001) ...
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bioRxiv - Cell Biology 2023Quote: MCF-10A cells (ATCC) and derivatives were cultured in Dulbecco’s Modified Eagle Medium Nutrient Mixture F-12 Ham 1:1 powder (Gibco) supplemented with 5% horse serum (Invitrogen) ...
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bioRxiv - Plant Biology 2023Quote: ... 23°C) and separated on linear gradient 4-12% Bis-Tris polyacrylamide gels (NuPAGE® Novex®, Invitrogen, USA) in MES running buffer (50 mM MES ...
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bioRxiv - Neuroscience 2023Quote: ... 10 μg of protein from extracts were separated in NuPAGE 4-12% Bis-Tris Gel 1.0mm (NP0321BOX, Thermo Scientific) or 3-8% Tri-Acetate gel (EA03755BOX ...
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bioRxiv - Cell Biology 2023Quote: ... from day 0 to day 2 the media was switched to a Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM F12, Gibco) based medium ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant (30 μl) was loaded on a NuPAGE Bis-Tris 4-12% polyacrylamide gel (Invitrogen, Massachusetts, United States), and run in MOPS buffer at 200 V ...
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bioRxiv - Physiology 2023Quote: ... Skin tissues were shaved and cleaned with 70% ethanol then minced and incubated in DMEM/F-12 medium (ThermoFisher) with Liberase (Millipore Sigma ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... organoids were passaged using H+++B Medium (Advanced DMEM/F-12 [Thermo Fisher], HEPES pH7.2-7.5 [10mM, Thermo Fisher] ...
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bioRxiv - Immunology 2023Quote: ... mounted onto Epredia Polysine Slides (Fisher 12-545-78) using prolong gold antifade mounting reagent (Thermo Fisher Cat# P36934) then covered with No.25 Glass Coverslips (Epredia Cat# 24×50-1.5-001G).
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bioRxiv - Genetics 2023Quote: Proteins were separated by SDS-PAGE electrophoresis on a Bolt 4-12% Bis-Tris Plus Gel (Thermo Fisher Scientific) and then transferred onto a 0.45-µm nitrocellulose membrane in a Mini Trans-Blot Cell (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... 20Lμg of cell protein extract per sample was separated on 4-12% Bolt® Bis-Tris Plus gels (ThermoFisher) using MOPS SDS NuPAGE Running Buffer (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... qPCR analysis of 12 genes was performed with TaqMan™ gene expression array assay (ThermoFisher Scientific, Cat. No. 4331182) using the Quant-Studio-5 (Applied Biosystems) ...
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bioRxiv - Bioengineering 2023Quote: ... 15 μg of each sample was loaded onto a 4-12% Bis-Tris SDS-PAGE gel (Thermo Fisher, WG1401A). Two μL of MagicMark XP Western Protein Standard (Thermo Fisher ...
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bioRxiv - Molecular Biology 2023Quote: ... 50μg of lysate was mixed with SDS loading buffer and loaded into 4%-12% precast gels (NP0335, Life Technologies). For BRCA1 western blotting ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins in cell lysates (20-40 μg) were separated on precast NuPAGE™ 4-12% Bis-Tris gels (Invitrogen) using MES (Figure 4 ...
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bioRxiv - Developmental Biology 2023Quote: ... Separation of metabolites was achieved in 12 minutes using a Hypercarb column (5 mm, 100X2.1 mm column, Thermo Scientific) and a gradient run of solvent A (7.5 mM ammonium acetate with 0.05% ammonium hydroxide ...
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bioRxiv - Biophysics 2023Quote: ... Total cell lysates were prepared and analyzed by SDS-PAGE using 4-12% gradient Bis-Tris NuPAGE gels (ThermoFisher) followed by immunoblotting71 ...
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bioRxiv - Cancer Biology 2023Quote: ... 50 μg of protein were resolved using a NuPAGE (4– 12%) Bis-Tris gel (Thermo-Fisher Scientific, Cat. # NP0322PK2). After the run ...
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bioRxiv - Microbiology 2023Quote: ... Necleofected macrophages plated in 12-well plates were lysed by incubating in RIPA buffer (Thermo Fisher Scientific, Cat#89900) for 20 min on ice and protein concentration was measured by BCA assay ...
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Analysis of spike protein variants evolved in a novel mouse model of persistent SARS-CoV-2 infectionbioRxiv - Microbiology 2023Quote: ... Equal amounts of protein were loaded and resolved on a 4–12% Bis-Tris gradient gel (Thermo Fisher Scientific) and analyzed by western blot with anti-SARS-CoV-2 N protein monoclonal antibody (0.5 μg/mL ...
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bioRxiv - Microbiology 2023Quote: ... Equal amounts of protein samples within each group were loaded and resolved using 4–12% NuPAGE gels (Life Technologies), and subsequently electro-transferred onto nitrocellulose membrane (iBlot ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were prepared and run on NativePage 4-12% Bis-Tris gels as per the manufacturer’s instructions (Life Technologies). For immunoblotting ...
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bioRxiv - Cell Biology 2023Quote: Lysates containing 25 µg of protein were electrophoresed on a NuPage 4%-12% Bis-Tris Protein gel (Thermofisher Scientific). Proteins were transferred to a PVDF membrane (0.2 µm ...
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bioRxiv - Neuroscience 2023Quote: PC-12 and Ins-1 cells were transfected using lipofectamine 2000 as per manufacturers’ instructions (Invitrogen, Carlsbad, California, USA). After transfection ...
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bioRxiv - Microbiology 2023Quote: ... The proteins were solubilized in Laemmli buffer and stacked in the top of a 4-12% NuPAGE gel (Invitrogen). After staining with R-250 Coomassie Blue (Biorad) ...