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Citations for Thermo Fisher :
5701 - 5750 of 10000+ citations for Anti Epidermis type lipoxygenase 12 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... the cells were transitioned to CNCC early maintenance media: 1:1 Neurobasal medium/D-MEM F-12 medium (Invitrogen), 1X penicillin-streptomycin solution ...
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bioRxiv - Neuroscience 2024Quote: ... 10 μg of total protein per sample was loaded onto 4 to 12% bis-tris SDS- polyacrylamide gel (Invitrogen). Following electrophoresis ...
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bioRxiv - Microbiology 2024Quote: ... 30 μL of each concentrated sample was run on a Bolt 4–12% Bis-Tris Plus Gel (Thermo Scientific, catalog number ...
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bioRxiv - Cell Biology 2024Quote: ... Lysates (35-40 µg) were separated by SDS-PAGE on NuPage 4-12% gels (Bis-Tris Gel; Invitrogen, #NP0335BOX), transferred to polyvinylidene fluoride membranes and blocked in 5% (w/v ...
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bioRxiv - Cell Biology 2024Quote: ... or C12E8 (0.5 mg/mg protein) and purified by a brief SDS-PAGE run (Nu-PAGE 4-12%, Invitrogen). Lanes were excised ...
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bioRxiv - Cell Biology 2024Quote: ... and aliquots containing 5 × 106 cells were separated on a NuPAGE bis-tris 4–12% gradient polyacrylamide gel (Invitrogen). The sample lane was divided into eight slices that were excised from the Coomassie-stained gel ...
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bioRxiv - Cancer Biology 2024Quote: ... human mammary fibroblast (MF) and cancer associated fibroblast (CAF) were maintained in DMEM/F-12 (Thermo Fisher Scientific, #21331046) supplemented with 2 mM Glutamine (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... Real Time PCR was performed with Applied Biosystems Fast SYBR Green Master Mix (Thermo Fisher Scientific, 43-856-12). RNA for microRNA expression analysis was isolated using the miRNeasy Micro Kit (Qiagen 1071023) ...
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bioRxiv - Neuroscience 2024Quote: Neuroblastoma SH-SY5Y cells were cultured in Dulbecco’s modified Eagle’s medium with GlutaMAX (DMEM/F-12, Gibco, UK, 10566016) supplemented with 10% (v/v ...
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bioRxiv - Bioengineering 2023Quote: ... the basal medium was changed to LaSR medium (Advanced DMEM/F-12, Life Technologies, cat# 12634, GlutaMAX, Thermo Fisher, cat# 35050-061 ...
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bioRxiv - Cancer Biology 2024Quote: ... 100,000 U2OS cells were seeded in a 12-well plate in antibiotic-free Dulbecco’s Modified Eagle Medium (DMEM, Thermofisher) supplemented with 10% FBS ...
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bioRxiv - Molecular Biology 2024Quote: ... and samples containing 30 µg total protein were analyzed by western blotting on 4-12% Bis-Tris Gels (Invitrogen). Proteins were transferred onto nitrocellulose membranes in accordance with the manufacturer’s instructions (Biorad) ...
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bioRxiv - Genomics 2024Quote: ... The ligated sheared 3C libraries (10-12 µg for each replicate) were pulled down using Streptavidin Dynabeads (Thermo Scientific) to get rid of unligated fragments ...
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bioRxiv - Genetics 2024Quote: Unfixed bone marrow smears were diffusely spread on Fisherbrand™ SuperfrostTM glass slides (Fisher Scientific, catalog # 12-550-14G) without heparin treatment in the dark ...
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bioRxiv - Cancer Biology 2024Quote: ... 10–15 µL of protein lysis were run per lane of 4–12% Bis-Tris gels (Thermo Fisher Scientific) using MOPS buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were separated by electrophoresis in NuPage 4-12% Bis-tris gels run with 1X MOPS buffer (Invitrogen NP0323) and transferred to an iBlot nitrocellulose membrane (Invitrogen IB301002) ...
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bioRxiv - Cell Biology 2024Quote: ... 10 µg protein sample was loaded in each well and ran on a 4%–12% SDS-PAGE gel (Invitrogen), then transferred to the nitrocellulose membrane (0.2 µm ...
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Reduction of chromosomal instability and inflammation is a common aspect of adaptation to aneuploidybioRxiv - Cell Biology 2023Quote: ... Cells were cultured at 37C at 5% CO2 in Advanced Dulbecco’s Modified Eagle Medium: Nutrient mixture F-12 (DMEM-F12) with Glutamax (GIBCO), supplemented with 12% FCS (Clontech) ...
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bioRxiv - Cell Biology 2024Quote: ... while 10 μg/lane of lysates were electrophoresed on Bolt 4–12% Bis-Tris Plus Gels (Thermo Fisher Scientific) for β-actin ...
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bioRxiv - Immunology 2024Quote: ... PC3 cells were cultured in F-12K Medium (Kaighn’s Modification of Ham’s F-12 Medium) (GIBCO, ref. 21127– 022), supplemented with 10% Fetal Bovine Serum (Corning ...
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bioRxiv - Microbiology 2024Quote: ... the bound proteins resolved using 4-12% Novex Tris-glycine gels and silver-stained (MS compatible) (Thermo Fisher Scientific). Trypsin digestions of the 0.2% FA elution were processed for mass spectrometric (MS ...
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bioRxiv - Immunology 2024Quote: RNA was purified from various T cell subsets cultured under Th0 conditions for 12 h using Trizol reagent (Invitrogen). cDNA was synthesized using reverse transcription kit (Progema) ...
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bioRxiv - Immunology 2024Quote: ... MoDCs were removed from 12-well Nunclon™ Delta surface-treated flat bottom plates (Thermo Fisher Scientific, Waltham, MA), pelleted ...
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bioRxiv - Biochemistry 2023Quote: ... heated for 5 minutes at 95°C and loaded on 4-12% Bis-Tris NuPage gels (Thermo Fisher Scientific). After electrophoretic separation ...
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bioRxiv - Cancer Biology 2023Quote: ... and LCPNS-SIIN-Cxcl1KO cells were cultured at 37°C under 5% CO2 / 5% O2 in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F12 medium, Gibco) supplemented with 1% N2 (Gibco) ...
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bioRxiv - Genetics 2023Quote: Human hTERT-RPE-1 PAC knockout cells (gifted by Andrew Holland (Lambrus et al., 2016)) were grown in DMEM:F-12 (Gibco) supplemented with 10% FBS (VWR Scientific) ...
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bioRxiv - Biophysics 2024Quote: The RPE-1 cell lines were cultured using Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (Thermo Scientific 11320033) supplemented with 10% FBS (VWR Life Science 100% Mexico Origin 156B19) ...
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bioRxiv - Biochemistry 2024Quote: Reduced protein extracts were separated on 10% SDS–PAGE gels or 4–12% NuPAGE bis–tris precast gels (Invitrogen) by electrophoresis ...
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bioRxiv - Cell Biology 2024Quote: ... The 2D well of the pre-cast NuPAGE 4%-12% Bis-tris 2D well gel (Thermo Fisher Scientific, NP0326BOX) was washed and filled with 1x MOPS running buffer (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2024Quote: ... 10 µm axial cryostat sections were placed on Fisherbrand™ Superfrost™ slides (Fisher Scientific, catalog # 12-550-14G), air dried for 20 minutes at room temperature (RT) ...
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bioRxiv - Systems Biology 2023Quote: ... siRNA transfections in 12-well plate for measuring changes in secreted factors were carried out using Lipofectamine RNAiMax (Invitrogen) according to manufacturer instructions.
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bioRxiv - Cancer Biology 2023Quote: ... 15µg of each lysate prepared from muscle was loaded on NuPAGE 4-12% Bis-Tris acrylamide gels (Life Technologies) and run at 80V for 8 min to stack proteins in a single band ...
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bioRxiv - Neuroscience 2023Quote: ... The samples were centrifuged at 13,300 rpm for 10 minutes and ran on NuPage 4-12% Bis-Tris polyacrylamide gels (Invitrogen). Proteins were transferred onto a Immobilon PVDF membrane (Millipore) ...
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bioRxiv - Microbiology 2023Quote: ... the specimens were cut at 20-μm thickness and mounted on positively charged slides (Fisher Scientific #12-550-15). Routine H&E staining was performed to evaluate the morphological change of the placentas ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were washed with 3X PBS-T prior to mounting on Superfrost Plus slides (Fisher Scientific; 12-550-15) with Fluoromount-G (SouthernBiotech ...
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bioRxiv - Cell Biology 2023Quote: ... 12 µL of each sample was loaded on an SDS-PAGE (Novex WedgeWell 10-20% Tris-Glycine gels, Invitrogen), run for 50 minutes at 200 V ...
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bioRxiv - Molecular Biology 2023Quote: MCF10A cells (ATCC, CRL-10317) were subcultured in Dulbecco’s modified Eagles’ medium/nutrient mixture F-12 (Gibco, 1130-032) containing horse serum (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... Human A549 cells (ATCC CCL-185) were purchased from ATCC and maintained in Dulbecco’s Modified Eagle Medium (DMEM)/F-12 (Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2023Quote: ... eluted in sterile H2O and transfected into HEK293T cells seeded in 12-well plates using Lipofectamine 3000 reagent (Invitrogen)(19 ...
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bioRxiv - Microbiology 2023Quote: ... an equal amount per sample of 12 µg was boiled with Novex NuPAGE LDS Sample Buffer (Invitrogen, Carlsbad, CA) and loaded onto a 10-well Novex NuPAGE 4% to 12% BT SDS-PAGE gel (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... The samples were centrifuged at 13,300 rpm for 10 minutes and ran on NuPage 4-12% Bis-Tris polyacrylamide gels (Invitrogen). Proteins were transferred onto a Immobilon PVDF membrane (Millipore) ...
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bioRxiv - Microbiology 2022Quote: siRNA silencing was performed by transfecting 2.5 pmol of each siRNA per well in a 12-well plate into the RAW264.7 cells using Lipofectamine RNAi Max (13778150, ThermoFisher) according to manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... Whole synovium from a single knee was placed into a seated 12-well trans-well insert (Fisher Scientific, 353494) with serum free 1:1 DMEM/F12 with 1% penicillin-streptomycin in both chambers and equilibrated for 24 hours before transfer into co-culture ...
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bioRxiv - Neuroscience 2023Quote: ... composed of Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 + GlutaMAX™ supplement (DMEM+GlutaMAX™, #31331028, Gibco™) supplemented with 100U/mL penicillin and 100μg/mL streptomycin (#15070063 ...
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bioRxiv - Cancer Biology 2023Quote: ... and the RPE1-hTERT TP53-/-;BRCA1-/-;H2AFX-/- clones were grown at 3% O2 in Dulbecco’s modified Eagle medium Nutrient mixture F-12 (DMEM/F12; Gibco) and penicillin/streptomycin solution (100 U/ml) ...
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bioRxiv - Neuroscience 2022Quote: Forty micrograms of protein was heated in the loading buffer and loaded on 5–12% gradient NuPage gels (Invitrogen). After the transfer on nitrocellulose membranes ...
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bioRxiv - Immunology 2023Quote: ... Samples were subjected to SDS-PAGE on 4-12% Tris-Glycine Gels (Bio Rad) and transferred to a 0.2 μm nitrocellulose membrane using the iBlot system (Invitrogen) followed by blocking with 5% Milk in Tris-buffered saline with Tween 20 (TBST ...
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bioRxiv - Microbiology 2023Quote: ... 5×104 HEK 293T and 2.5×104 MDCK-SIAT1 cells were plated in 12 well plates in growth media (DMEM, Invitrogen #19965-092 ...
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bioRxiv - Molecular Biology 2023Quote: The eluted proteins solubilized in Laemmli buffer were stacked in the top of a 4-12% NuPAGE gel (Invitrogen). After staining with R-250 Coomassie Blue (Biorad) ...
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bioRxiv - Microbiology 2022Quote: ... heated for 10 min at 95°C and separated on 4–12% Bis-Tris NuPAGE gels (Thermo Fisher Scientific) in the presence of antioxidant (Thermo Fisher Scientific) ...