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Citations for Thermo Fisher :
6001 - 6050 of 10000+ citations for Anti Epidermis type lipoxygenase 12 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... in an SDS running buffer (192 mM glycine, 25 mM Tris base, 0.1% SDS) or using NuPAGETM 4-12% gradient gels (Invitrogen WG1403BX10) in NuPAGETM MOPS-SDS running buffer (Invitrogen NP0001-02) ...
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bioRxiv - Microbiology 2020Quote: Approximately 2×105 of 293T control cells or 293T MTHFD1 knock out cells were plated into each well of 12-well plates and the knockout cells were cultured with HT (100 μM hypoxanthine and 16 μM thymidine, Gibco) medium ...
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bioRxiv - Neuroscience 2020Quote: ... or 30 μg (retinal extract) of proteins were loaded per lane on NuPAGE 4-12% (wt/vol) Bis-Tris gels (Invitrogen). Proteins were transferred onto a nitrocellulose membrane (Bio-Rad ...
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bioRxiv - Genetics 2020Quote: ... Samples were loaded on 4-12% NuPAGE Bis-Tris gels or 3-8% NuPAGE Tris acetate gels (Thermo Fisher Scientific) and transferred to the nitrocellulose membranes (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... and 30 μl of each sample was used to load duplicate NuPAGE 4-12% Bis-Tris acrylamide gels (Invitrogen, #NP0321) which were electrophoresed simultaneously ...
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bioRxiv - Immunology 2021Quote: ... Human dermal fibroblasts were generated from explant cultures of de-epidermized dermis as described40 and grown up to passage 4 in DMEM/F-12 (Thermofisher) complemented with 10% FBS and pen/strep antibiotics ...
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bioRxiv - Biophysics 2021Quote: ... was added to the samples and pellet fractions were run on a 4-12% BIS-Tris gel (Thermo Fisher Scientific). The gel was stained with EZBlue reagent (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: SDS-PAGE was performed using NuPAGE Novex 4-12% Bis-Tris gels according to the instructions of the manufacturer (Invitrogen). Gels were stained with SimplyBlue safe stain (Life technologies) ...
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bioRxiv - Immunology 2021Quote: Pneumococcal strains were mixed with non-reducing loading buffer (Laemmli SDS sample buffer, non-reducing 6X) and loaded on a 4-12% Bis-Tris gel (Invitrogen). Samples were then transferred to PVDF membranes via iBlot system (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... Transfections were carried out in a 12-well plate or 6-well plate using Lipofectamine 3000 (Invitrogen; Life Technologies Inc.) following the reverse transfection protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... Transfections were carried out in a 12-well plate or 6-well plate using Lipofectamine 3000 (Invitrogen; Life Technologies Inc.) following the reverse transfection protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... Precast gradient SDS-PAGE gels (NuPAGETM 4 to 12%, Bis-Tris 1.0 mm, Mini Protein Gel, 15-well, NP0323BOX, ThermoFisher SCIENTIFIC) were used to separate the proteins ...
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bioRxiv - Microbiology 2021Quote: Freshly lysed arrays were replica plated with 12 µL into 96- well plates of HFFs maintained in FluoroBrite DMEM (GIBCO) supplemented with 3% IFS ...
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bioRxiv - Neuroscience 2022Quote: ... and maintained in culture in Ham’s F-12 culture medium (PAA Laboratories) with 5% foetal bovine serum (Thermo Fisher Scientific). Cell culture media containing sNEGR1 secreted by the cells was collected and centrifuged for 15 min at 5000 g at 4°C to remove cell debris ...
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bioRxiv - Cell Biology 2022Quote: ... and penicillin/streptomycin solution (Thermo). mIMCD3 Flp-In cells (gift from D. Mick124) were cultured in DMEM/F-12 (Gibco) media supplemented with 2mM stable glutamine (Biowest) ...
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bioRxiv - Bioengineering 2022Quote: ... of cell extracts was separated by NuPAGE gel electrophoresis (4-12% Midi 1.0 mm Bis-Tris precast gels, Life technologies) and electrotransferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Biochemistry 2022Quote: ... Pulldown eluates and input controls were loaded in a 4-12% Bis-Tris gel and ran in NuPAGE MOPS SDS running buffer (Invitrogen) at 200V for 50 min in a XCell SureLock electrophoresis system (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... 10-30 μg of samples was loaded onto NuPAGE 4–12% Bis–Tris Midi Gel (Thermo Fisher Scientific, Cat# WG1403BOX) and electrophoresed with the NuPAGE MOPS SDS running buffer (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... 10 ul of each sample and 3% input of each cell lysate was loaded on a 4-12% Bis-Tris pre-cast SDS-page gel (Invitrogen) and transferred to a PVDF membrane (BioRad) ...
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bioRxiv - Molecular Biology 2021Quote: ... the cells were stimulated with phorbol 12-myristate 13 acetate (PMA) and ionomycin (Cell stimulation cocktail, eBioscience, ThermoFisher Scientific, USA), and cell supernatants were collected after 24 hours.
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bioRxiv - Molecular Biology 2021Quote: ... Plasmid pCR8-pilA1-ORF was recombined with plasmid pAX1-GW(12) (Table 3) using the Gateway LR recombination protocol (Invitrogen). The resulting plasmid ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were run at an increasing voltage (up to 120 V) in NuPAGE 4-12% BisTris gels in 1x MOPS buffer (Invitrogen). Proteins were transferred overnight to a 0.45 µm nitrocellulose membrane at 30 V ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were boiled at 95°C for 5 min before running on a NuPAGE 4-12% Bis-Tris protein gradient mini gel (Invitrogen) at 200 V for 5 min in MOPS running buffer (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was isolated from Hela cells (n = 3) subjected to EBSS-induced autophagy and treatment with 5 μM UNC0638 for 12 hours using TRIzol reagent (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: Cells were lysed in RIPA buffer and 20 μg of protein lysate were separated on NuPAGE 4-12% Bis-Tris Midi Gels (Invitrogen). Proteins were transferred onto nitrocellulose membrane and stained with Ponceau Red to confirm complete transfer ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein samples were separated on a NuPAGE™ 4–12% Bis-Tris Protein Gel (Thermo Fisher Scientific, Bleiswijk, the Netherlands) and transferred to an Immobilon-FL PVDF membrane (Merck ...
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bioRxiv - Cell Biology 2022Quote: ... 1-1.6×106 cells were seeded on 0.1% gelatine-coated 10 cm plates in N2B27 medium composed of 50% Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F12) / 50% Neurobasal Medium (Life Technologies), supplemented with 1% B-27 supplement (Life Technologies) ...
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Endothelial transmigration hotspots limit vascular leakage through heterogeneous expression of ICAM1bioRxiv - Immunology 2022Quote: ... Samples were boiled at 95ᵒC for 3 minutes to denature proteins and separated on a 4-12% NuPage Bis-Tris gel (Invitrogen, NP0322BOX). Proteins were transferred using an iBlot Gel Transfer device (Invitrogen ...
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bioRxiv - Genomics 2020Quote: 20μl of each cellular fraction was used for SDS-PAGE in a NuPAGE 4-12% Bis-TRIS gel and MOPs running buffer (ThermoFisher). For subcellular fraction marker proteins ...
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bioRxiv - Developmental Biology 2020Quote: Single cells were dissociated from E12.5 embryonic forebrains and cultured in suspension in DMEM/F-12 (Life Technologies, 10565-042) containing N2 supplement (Life Technologies ...
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bioRxiv - Immunology 2020Quote: ... and 20 mg/ml Proteinase K at 56°C for 12 h followed by genomic DNA extraction with phenol/chloroform/isoamyl alcohol (Invitrogen), non-fluorescent pellet paint (Millipore ...
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bioRxiv - Genomics 2020Quote: ... the medium is replaced with 100 ml fresh medium plus 10 ml of a 12 mM MTT stock solution (Invitrogen), and cells are incubated at 37°C for 4 h ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... These cells were grown in a 1:1 mixture of Ham’s F-12 medium (cat# 11765-054, Invitrogen, Camarillo, CA) and 3T3 fibroblast preconditioned Dulbecco’s modified eagle medium supplemented with insulin (10 μg/ml ...
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bioRxiv - Molecular Biology 2020Quote: ... the samples were separated on a 4–12% gradient Bis-Tris gel (NuPAGE Bis-Tris gels, 1.0 mm, 10 well; Art. No. NP0321; Life Technologies) in 1X MOPS (NuPAGE 20X MOPS SDS running buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... Thirty μg of protein in each sample was resolved by electrophoresis using 4-12% bis-tris gels (Thermo Fisher Scientific) and transferred to nitrocellulose membrane (Bio-Rad ...
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bioRxiv - Biochemistry 2021Quote: ... Reaction products were separated by gel electrophoresis on 4-12% Bis-Tris gradient gels using MES SDS running buffer (Invitrogen) and stained with Flamingo fluorescent protein stain (Bio-Rad) ...
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bioRxiv - Biochemistry 2020Quote: Gel electrophoresis was performed using 4-12% NuPAGE Bis-Tris gels according to manufacturer’s protocols (NuPAGE system, Thermo Fisher Scientific). Protein gel bands were excised ...
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bioRxiv - Genomics 2020Quote: ... Cell lysates dissolved in SDS load buffer were heated at 90°C for 3 min and separated on a Novex WedgeWell 4-12% Tris-Glycine Gel (Invitrogen). Proteins were transferred to a PVDF Transfer Membrane (Thermo Scientific ...
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bioRxiv - Genetics 2020Quote: ... adhesion matrix (1:100 in DMEM/F:12) and cells were seeded at a density of 40,000 cells cm-2 in Stemflex media (Gibco, A3349401) containing Genetecin as selective antibiotic (Thermo Fisher ...
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bioRxiv - Immunology 2021Quote: ... cells were counted and plated in a 12 well plate (7.5 x 104-1.0 x 105 cells/well) coated with collagen (Thermo Fisher Scientific). Submerged MTEC cultures were incubated at 37 °C in a humidified incubator containing 95% air ...
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bioRxiv - Genomics 2020Quote: Total protein from equal amounts of cells was separated on a NuPage Gradient 4-12% Bis-Tris Mini Gel (Invitrogen), transferred to a PVDF membrane ...
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bioRxiv - Genomics 2021Quote: Fresh-frozen VP tissues from 12-week-old mice were sliced on ice with stainless steel disposable scalpels (Fisher Scientific) then homogenized in RIPA buffer (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Immunology 2021Quote: ... of WT and Adrb2-/- Cl4 mice (7-12 weeks old) with a negative isolation kit according to the manufacturer’s instructions (Invitrogen #11417D). Donor T cells were derived from CD90.1 congenic animals in order to track their frequency in CD90.2 hosts ...
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bioRxiv - Neuroscience 2021Quote: ... 25 µl of supernatant was mixed with loading buffer and resolved by SDS-PAGE in 4- 12% Bis–Tris gels (Invitrogen) under reducing conditions and electro-blotted onto nitrocellulose membranes ...
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bioRxiv - Microbiology 2021Quote: ... Samples were heated to 50°C for 5 minutes before being loaded on 4-12% Novex WedgeWell Tris-Glycine gels (ThermoFisher).
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bioRxiv - Microbiology 2021Quote: ... Samples were heated to 50°C for 5 minutes before being loaded on 12% Novex WedgeWell Tris-Glycine gels (ThermoFisher). Protein bands were stained with an InstantBlue Coomassie solution (Abcam ...
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bioRxiv - Biophysics 2021Quote: Purity of protein constructs was checked by running them on a 12% SDS-PAGE gel followed by staining with Coomassie blue (SimplyBlueStain, Invitrogen) and imaging on a ChemiDoc MP (BioRad).
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bioRxiv - Cell Biology 2020Quote: ... Laemmli sample buffer (0.1 mL of 4x) was added to lysate prior to loading 10 μL on a 4%–12% Bis-Tris NuPAGE gel (Invitrogen). Gels were transferred onto a Mini-size polyvinylidene difluoride membrane (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... WCE and bound proteins were separated by running in a 4-12% Bis-Tris gradient gel with MOPS SDS running buffer (Invitrogen). Mif2-TAF and Flag-Cse4 were detected by immunoblotting with anti-protein A (Sigma ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples (10-20 μg) were run on SDS/PAGE with NuPAGE precast 4-12% gradient gels (Invitrogen; Carlsbad, CA, USA) and blots were incubated overnight at 4°C with primary antibodies (Supplementary Table 1 ...