Labshake search
Citations for Thermo Fisher :
5701 - 5750 of 10000+ citations for rno mir 542 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Physiology 2020Quote: ... Gene expression analysis was then performed by quantitative PCR on a Quantstudio 6 Flex Real-time PCR System (Applied Biosystems, Foster City, CA) using the mouse primers shown in Table 1 ...
-
bioRxiv - Cell Biology 2022Quote: ... Quantitative RT-PCR were performed with TaqMan Gene Expression Assays (Applied Biosystem, Waltham, MA) on a StepOnePlus Real-Time PCR System (Applied Biosystems, Waltham, MA). Relative target gene expression levels were calculated using the delta-delta CT method(Livak & Schmittgen ...
-
bioRxiv - Cell Biology 2022Quote: ... Real-time PCR was done in triplicate with the QuantStudio 5 Real-time PCR system and Fast SYBR® Green Master Mix (Thermo Fisher Scientific) with 500 nM primers ...
-
bioRxiv - Genomics 2022Quote: ... The product was diluted 1:2 in RNase-free water and subjected to quantitative real-time PCR (qRT-PCR) using gene-specific TaqMan assays (Thermo Fisher Scientific 4331182) for mouse TTR (Mm00443267_m1) ...
-
bioRxiv - Systems Biology 2022Quote: ... The bacterial load present in the purified samples was measured by quantitative PCR (StepOnePlus Real-Time PCR System, Thermo Fisher Scientific, the Netherlands), using a universal primer set targeting the 16S rRNA gene (forward Eub34lF ...
-
bioRxiv - Cell Biology 2024Quote: ... DNA was purified using a QIAquick PCR Purification kit and qPCR was performed with Power Up SyBr Green PCR master mix using the ABI 7900HT Real Time PCR system (Applied Biosystems, Waltham, MA). PCR reactions contained primers for murine Vcan Prom400 (mVCAN Prom 400 ...
-
bioRxiv - Biochemistry 2024Quote: ... Real-Time Quantitative PCR (qPCR) was performed with the standard protocols in ABI 7500 Fast Real-Time PCR System (Applied Biosystems™ 7500, ThermoFisher, USA) using SYBR Green Master Mix ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... qRT-PCR was performed using the PowerUp SyBr Green Master Mix on the QuantStudio™ 3 Real-Time PCR System (Applied Biosystems, Thermo Scientific). Amplification was performed using the rapid cycling mode following the manufacturer’s specifications.The primers were designed based on nucleic acid sequences obtained from the National Center for Biotechnology Information and the primer sequences are displayed in Supplementary Table S1 ...
-
bioRxiv - Cancer Biology 2023Quote: ... Quantitative RT-PCR was performed with TaqMan Gene Expression Assays (Applied Biosystem, Waltham, MA) on an StepOnePlus Real-Time PCR System (Applied Biosystems, Waltham, MA). Relative target gene expression levels were calculated using the delta-delta CT method ...
-
bioRxiv - Bioengineering 2022Quote: ... vRNA levels were determined by quantitative real-time PCR (qRT-PCR) using Applied Biosystems™ TaqMan™ Fast Virus 1-Step Master Mix (Thermofisher #4444432) and a primer-probe combination recognizing a conserved region of gag (GAG5f ...
-
bioRxiv - Molecular Biology 2023Quote: ... Real-time polymerase chain reaction (PCR) was performed using the Vazyme System (Vazyme, Nanjing, China) on a 7900HT FAST Real-time PCR System (Life Technologies, Carlsbad, CA). The level of genes was calculated based on the cycle threshold (Ct ...
-
bioRxiv - Neuroscience 2023Quote: ... Transcript levels of candidate genes were measured by qRT-PCR using cDNA with the QuantStudio 6 Flex Real-Time PCR System (Life Technologies, Darmstadt, Germany) according to the company’s guidelines.
-
bioRxiv - Genetics 2024Quote: ... and TaqMan Assay probes were loaded in technical quadruplicates for qRT-PCR on a QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher Scientific, 4485691). The ΔΔCt method was used to provide gene expression values after normalizing to the known reference gene Gapdh ...
-
bioRxiv - Neuroscience 2024Quote: ... The resulting cDNA was used to quantify the SARS-CoV-2 RNA levels by real-time quantitative PCR using Power SYBR green PCR master mix (Applied Biosystems, Waltham, MA). Average values from duplicates of each sample were used to calculate the viral RNA level relative to the GAPDH gene and presented as 2−ΔCT ...
-
bioRxiv - Cell Biology 2024Quote: ... was performed on 12 ng cDNA with SybrGreen PCR mastermix (LifeTechnologies) and specific primers (supplementary Table 2) and run in an ABI? real-time PCR machine (Applied Biosystems, ThermoFisher Scientific). The raw data was analysed through -ΔCt as described in Supplementary Methods.
-
bioRxiv - Biochemistry 2024Quote: ... was performed on the cDNA (diluted 1 in 5 in nuclease-free water) using the Power SYBR Green PCR Master Mix and the StepOnePlus Real-Time PCR System (both Applied Biosystems, Warrington, UK). MALAT1 expression was normalised to RPL10 (60S ribosomal protein L10 ...
-
bioRxiv - Plant Biology 2024Quote: ... qRT-PCR was carried out using THUNDERBIRD SYBR qPCR Mix (TOYOBO, Osaka, Japan) on 7300 Real-Time PCR System (Applied Biosystems, Massachusetts, USA). For normalization ...
-
bioRxiv - Genomics 2024Quote: ... cDNA was diluted 10-fold and subjected to quantitative PCR using the PrimeTime™ Gene Expression Master Mix (Integrated DNA Technologies, Inc.) and QuantStudio 3 Real-Time PCR System (Thermo Fisher Scientific). Primers and probes (Integrated DNA Technologies ...
-
bioRxiv - Genomics 2021Quote: ... and the hrdB gene was used as the endogenous control (StepOne Plus real-time PCR system, Applied Biosystems). The sequences of optimized oligonucleotides used in this study are listed in Supplementary Table 2).
-
bioRxiv - Cancer Biology 2021Quote: ... Amplification was monitored using SYBR Green dye on a QuantStudio™ 3 Real-Time PCR System machine (ThermoFisher). All measurements were performed in triplicate and calculated using the ΔΔct method relative to GAPDH expression.
-
bioRxiv - Cell Biology 2020Quote: ... and specific primers on a 7,500 fast real-time PCR system (Applied Biosystems, Life Technologies, Waltham, MA, USA). The primer sequence for specific transcripts were as followed ...
-
bioRxiv - Cell Biology 2020Quote: ... and specific primers on a 7,500 fast real-time PCR system (Applied Biosystems, Life Technologies, Waltham, MA, USA). The primer sequence for specific transcripts were as followed ...
-
bioRxiv - Cell Biology 2020Quote: ... was used and qPCR was performed on a StepOnePlus Real time PCR System (Applied Biosystems, Foster City, CA). Primers for qPCR:
-
bioRxiv - Immunology 2021Quote: ... with 0.8 μM of forward and reverse primers on a QuantStudio 5 Real-Time PCR System (Applied Biosystems) with the following cycling conditions ...
-
bioRxiv - Genetics 2021Quote: ... Real-time quantitative PCR was then performed on resulting cDNAs in a Step-One Plus system (Applied Biosystems) using PerfeCTa SYBR Green FastMix ROX (Quanta BioSciences) ...
-
Differential polysaccharide utilization is the basis for a nanohaloarchaeon : haloarchaeon symbiosisbioRxiv - Microbiology 2019Quote: The qPCR was performed with SYBR Green on an ABI Prism 7300 Real-time PCR System (Applied Biosystems). All amplifications were checked for specificity with dsDNA melt curves and samples exhibiting multiple products were not considered in the analysis ...
-
bioRxiv - Developmental Biology 2021Quote: ... Real-time PCR was performed as triplicates on 2 ng of cDNA using SYBR Green master mix (ThermoFisher) on a ViiA 7 real-time PCR System (ThermoFisher) ...
-
bioRxiv - Molecular Biology 2022Quote: ... according to the manufacturer’s instructions in optical plates on a QuantStudio 5 Real-Time PCR system (ThermoFisher Scientific) equipped with 384-well block ...
-
bioRxiv - Pathology 2022Quote: ... on an Applied Biosystems StepOnePlus real-time PCR system (Life Technologies Ltd., Thermo Fisher Scientific, Waltham, MA, USA). From the qPCR ...
-
bioRxiv - Cancer Biology 2022Quote: ... qPCR reactions were performed on a Step One Plus Real-Time PCR System (Applied Biosystems, Thermo Fisher Scientific) using the THUNDERBIRD qPCR Mix (QPS-201 ...
-
bioRxiv - Cancer Biology 2022Quote: ... qPCR reactions were performed on a Step One Plus Real-Time PCR System (Applied Biosystems, Thermo Fisher Scientific) using the THUNDERBIRD qPCR Mix (QPS-201 ...
-
bioRxiv - Neuroscience 2021Quote: ... Real-time PCR was performed using TaqMan Gene Expression Assay for rat β-actin (Actb, Rn00667869_m1, Applied Biosystems), rat Taste receptor type 1 member 3 (Tas1r3 ...
-
bioRxiv - Neuroscience 2021Quote: ... in a 384 well plate on a QuantStudio™ 6 Flex Real-Time PCR System (Thermo Fisher, A28139). Each PCR cycle had the following conditions for denaturation ...
-
bioRxiv - Neuroscience 2021Quote: ... was performed using the Applied Biosystems QuantStudio 3 Real-Time PCR system and analyzed with the QuantStudio 3 Design and Analysis software (v1.5.1,ThermoFisher). Briefly ...
-
bioRxiv - Molecular Biology 2021Quote: ... Quantitative Real Time PCR was performed with Maxima SYBR Green/ROX qPCR Master Mix (Thermo Fisher cat# K0222) in a StepOnePlus RT-PCR System (Thermo Fisher).
-
bioRxiv - Molecular Biology 2021Quote: ... Occupancy of S2P RNAPII was monitored with qPCR (Thermo PikoReal 96 Real-Time PCR system; Thermo Fisher Scientific). Sequences of primers used for qPCR are listed in Table S1.
-
bioRxiv - Cell Biology 2022Quote: ... Relative mRNA expression levels were quantified using SYBR green-based quantitative real-time PCR (Applied Biosystems, Darmstadt, Germany) using the 7900GR fast real-time PCR system (Applied Biosystems) ...
-
bioRxiv - Microbiology 2019Quote: The qPCR assays were performed on a QuantStudio 3 Real-Time PCR System (ThermoFisher Scientific, Cat. No. A28567) in 20 µL reaction volume including ...
-
bioRxiv - Biophysics 2019Quote: ... The temperature was ramped from 4 to 95 °C in a ViiA7 real-time PCR machine (Applied Biosystems). The protein melting temperature ...
-
bioRxiv - Cell Biology 2019Quote: ... Real-time PCR were performed using SyBr® Green Master Mix (Life Technologies®, Grand Island, NY, USA). The primer sequences are detailed in Supplementary Table 3 ...
-
bioRxiv - Systems Biology 2019Quote: ... qPCR reactions and measurements were performed on a StepOne Real-Time PCR System (Thermo Fisher, cat. no. 4376357) according to the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2019Quote: ... FZD7 and ELP3 (all from reference 65) (Table S3) using a ViAA7 Real Time PCR System (ThermoFisher Scientific). Specificity of antibodies was validated using the isotype controls and samples were normalized to their corresponding input controls.
-
bioRxiv - Pathology 2019Quote: ... according to manufacturer’s instructions and assayed in 7500 Fast Real-Time PCR cycler (Applied Biosystems Inc, CA, USA) under the following thermal cycling conditions ...
-
bioRxiv - Cell Biology 2020Quote: ... Gene-specific primers were used to perform qPCR on the ABI 7500 Real-Time PCR System (Applied Biosystems) with MESA GREEN qPCR MasterMix Plus and SYBR® Assay Low ROX (Eurogentec ...
-
bioRxiv - Immunology 2019Quote: ... and 45 cycles at 55°C for 1 min on 7900HT Fast Real-Time PCR System (Applied Biosystems). Assay exclusivity of EV71-VP1 was confirmed by testing viral RNA extracted from dengue viruses (DENV-1 to DENV-4) ...
-
bioRxiv - Cancer Biology 2020Quote: ... qRT-PCR was performed using TaqMan assays (Col1a2 Mm00483888_m1, Acta2 Mm00725412_s1 and Gapdh Mm99999915_g1) and a StepOne Real Time PCR System (both Life Technologies). Levels of each gene was expressed as 2-δCT (relative to Gapdh).
-
bioRxiv - Evolutionary Biology 2020Quote: ... We ran 10 plates (384-well format) on QuantStudioTM 12K Flex Real-Time PCR System (Thermo Fisher Scientific). Each 10 μL reaction contained 200 nM of each primer ...
-
bioRxiv - Molecular Biology 2020Quote: ... Expression levels of mRNA were performed with either an ABI Prism 7900HT Real-Time PCR System (Applied Biosystems) or a QuantStudio 5 Real-Time PCR System (Applied Biosystems).
-
bioRxiv - Microbiology 2021Quote: ... mRNA expression of the indicated genes was analyzed by QuantStudio 7 Flex Real Time PCR System (Applied Biosystems) using SYBR green master mix ...
-
bioRxiv - Microbiology 2021Quote: ... Culture-independent quantification of bacteria was performed by SYBR Green Real-Time PCR (Thermo Fisher Scientific, Emeryville, CA) to amplify the 16S rRNA gene in samples from mosquitoes and water ...